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anti mouse rbc monoclonal antibody  (Hycult Biotech)


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    Hycult Biotech anti mouse rbc monoclonal antibody
    Anti Mouse Rbc Monoclonal Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse rbc monoclonal antibody/product/Hycult Biotech
    Average 93 stars, based on 5 article reviews
    anti mouse rbc monoclonal antibody - by Bioz Stars, 2025-11
    93/100 stars

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    Altered intracellular survival of L. donovani in Atp6v0d2 -knockdown BMDMs with or without opsonized erythrocyte supplementation. BMDMs were transfected with either si-control or si-Atp6v0d2 siRNA for 24 hours before L. donovani infection. The number of intracellular amastigotes was counted in over 100 BMDMs. The infection index refers to the ratio of the number of intracellular amastigotes per BMDM in each group to that in sole L. donovani infection group. (A) Infection indexes of si-control or si-Atp6v0d2-treated BMDMs without opsonized RBC supplementation at 6 hours and 72 hours post initial infection. (B) BMDMs were incubated with opsonized RBCs for 2 hours before L. donovani infection. One group of cells was infected and supplemented with opsonized RBCs as the other groups while being additionally treated with DFP. Counting of intracellular amastigotes were performed at 72 hours of infection and infection indexes were calculated as described earlier. (C) BMDMs were incubated with opsonized RBCs for 2 hours before L. donovani infection. The proportion of cells with 2 or more nuclei in over 100 cells at 72 hours post infection is shown. Means + SD of three independent experiments are shown. * P < 0.05, ** P <0.01, *** P <0.001 by one-way ANOVA followed by Tukey’s multiple comparisons test.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Upregulation of ATP6V0D2 benefits intracellular survival of Leishmania donovani in erythrocytes-engulfing macrophages

    doi: 10.3389/fcimb.2024.1332381

    Figure Lengend Snippet: Altered intracellular survival of L. donovani in Atp6v0d2 -knockdown BMDMs with or without opsonized erythrocyte supplementation. BMDMs were transfected with either si-control or si-Atp6v0d2 siRNA for 24 hours before L. donovani infection. The number of intracellular amastigotes was counted in over 100 BMDMs. The infection index refers to the ratio of the number of intracellular amastigotes per BMDM in each group to that in sole L. donovani infection group. (A) Infection indexes of si-control or si-Atp6v0d2-treated BMDMs without opsonized RBC supplementation at 6 hours and 72 hours post initial infection. (B) BMDMs were incubated with opsonized RBCs for 2 hours before L. donovani infection. One group of cells was infected and supplemented with opsonized RBCs as the other groups while being additionally treated with DFP. Counting of intracellular amastigotes were performed at 72 hours of infection and infection indexes were calculated as described earlier. (C) BMDMs were incubated with opsonized RBCs for 2 hours before L. donovani infection. The proportion of cells with 2 or more nuclei in over 100 cells at 72 hours post infection is shown. Means + SD of three independent experiments are shown. * P < 0.05, ** P <0.01, *** P <0.001 by one-way ANOVA followed by Tukey’s multiple comparisons test.

    Article Snippet: Preparation of the opsonized RBCs were performed by incubating 2 × 10 7 murine erythrocytes with 0.5 μg of monoclonal anti-mouse red blood cell (RBC) antibodies (HM1120-FS, Hycult Biotechnology B.V., Netherlands) in DMEM for 1 hour followed by washing for 3 times.

    Techniques: Knockdown, Transfection, Control, Infection, Incubation