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mouse viperin  (Hycult Biotech)


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    Structured Review

    Hycult Biotech mouse viperin
    (A) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification <t>of</t> <t>GAPDH</t> mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of GAPDH protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (** P<0.01; *** P<0,001). (B) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of HPRT1 mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of HPRT1 protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001); n.d.–not detected. (C) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at the indicated time intervals. Relative qPCR quantification of <t>viperin</t> mRNA using Δ-c t method with normalisation to the cell number was performed. Data are summary of three independent experiments and values are expressed as mean with standard error of mean (SEM). Lower panel: Immunodetection of viperin protein in TBEV-infected DAOY cells at indicated intervals p.i. (MOI 5). As a positive control, cells transfected with a c-myc-tagged viperin expression plasmid (wt vip) and cells treated with IFN-β (12 hours; 50 ng/ml) were used.
    Mouse Viperin, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse viperin/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    mouse viperin - by Bioz Stars, 2025-02
    90/100 stars

    Images

    1) Product Images from "Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin"

    Article Title: Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0007745

    (A) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of GAPDH mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of GAPDH protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (** P<0.01; *** P<0,001). (B) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of HPRT1 mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of HPRT1 protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001); n.d.–not detected. (C) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at the indicated time intervals. Relative qPCR quantification of viperin mRNA using Δ-c t method with normalisation to the cell number was performed. Data are summary of three independent experiments and values are expressed as mean with standard error of mean (SEM). Lower panel: Immunodetection of viperin protein in TBEV-infected DAOY cells at indicated intervals p.i. (MOI 5). As a positive control, cells transfected with a c-myc-tagged viperin expression plasmid (wt vip) and cells treated with IFN-β (12 hours; 50 ng/ml) were used.
    Figure Legend Snippet: (A) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of GAPDH mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of GAPDH protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (** P<0.01; *** P<0,001). (B) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of HPRT1 mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of HPRT1 protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001); n.d.–not detected. (C) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at the indicated time intervals. Relative qPCR quantification of viperin mRNA using Δ-c t method with normalisation to the cell number was performed. Data are summary of three independent experiments and values are expressed as mean with standard error of mean (SEM). Lower panel: Immunodetection of viperin protein in TBEV-infected DAOY cells at indicated intervals p.i. (MOI 5). As a positive control, cells transfected with a c-myc-tagged viperin expression plasmid (wt vip) and cells treated with IFN-β (12 hours; 50 ng/ml) were used.

    Techniques Used: Infection, Isolation, Western Blot, Software, Immunodetection, Positive Control, Transfection, Expressing, Plasmid Preparation



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    Hycult Biotech mouse viperin
    (A) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification <t>of</t> <t>GAPDH</t> mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of GAPDH protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (** P<0.01; *** P<0,001). (B) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of HPRT1 mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of HPRT1 protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001); n.d.–not detected. (C) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at the indicated time intervals. Relative qPCR quantification of <t>viperin</t> mRNA using Δ-c t method with normalisation to the cell number was performed. Data are summary of three independent experiments and values are expressed as mean with standard error of mean (SEM). Lower panel: Immunodetection of viperin protein in TBEV-infected DAOY cells at indicated intervals p.i. (MOI 5). As a positive control, cells transfected with a c-myc-tagged viperin expression plasmid (wt vip) and cells treated with IFN-β (12 hours; 50 ng/ml) were used.
    Mouse Viperin, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse viperin/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    mouse viperin - by Bioz Stars, 2025-02
    90/100 stars
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    (A) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of GAPDH mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of GAPDH protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (** P<0.01; *** P<0,001). (B) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of HPRT1 mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of HPRT1 protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001); n.d.–not detected. (C) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at the indicated time intervals. Relative qPCR quantification of viperin mRNA using Δ-c t method with normalisation to the cell number was performed. Data are summary of three independent experiments and values are expressed as mean with standard error of mean (SEM). Lower panel: Immunodetection of viperin protein in TBEV-infected DAOY cells at indicated intervals p.i. (MOI 5). As a positive control, cells transfected with a c-myc-tagged viperin expression plasmid (wt vip) and cells treated with IFN-β (12 hours; 50 ng/ml) were used.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin

    doi: 10.1371/journal.pntd.0007745

    Figure Lengend Snippet: (A) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of GAPDH mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of GAPDH protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (** P<0.01; *** P<0,001). (B) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at indicated time intervals. Relative qPCR quantification of HPRT1 mRNA using Δ-c t method with normalisation to the cell number was performed. Lower panel: DAOY cells were infected with either Neudoerfl or Hypr TBEV strain (5 MOI) and lysed at 48 hours p.i. Western blot analysis of HPRT1 protein levels was performed using protein-specific antibodies with undiluted and 10-times diluted samples. Relative chemiluminescent signal was quantified using Fiji software and compared to mock-infected cells. Values were further normalised to the cell number. Data are summary of three independent experiments and values in graphs are expressed as mean with SEM. Significant difference from mock-infected cells was calculated using one-sample Student’s t-test (* P<0.05; ** P<0.01; **** P<0,0001); n.d.–not detected. (C) Upper panel: DAOY cells were infected with either TBEV Neudoerfl or Hypr strain (MOI 5) and total RNA was isolated at the indicated time intervals. Relative qPCR quantification of viperin mRNA using Δ-c t method with normalisation to the cell number was performed. Data are summary of three independent experiments and values are expressed as mean with standard error of mean (SEM). Lower panel: Immunodetection of viperin protein in TBEV-infected DAOY cells at indicated intervals p.i. (MOI 5). As a positive control, cells transfected with a c-myc-tagged viperin expression plasmid (wt vip) and cells treated with IFN-β (12 hours; 50 ng/ml) were used.

    Article Snippet: The following primary antibodies were used: anti-TBEV C polyclonal antibody (produced in-house), anti-TBEV NS3 polyclonal antibody (a kind gift from Dr. M. Bloom, NIAID, USA), anti-HPRT1 Polyclonal Antibody (Thermo Fisher Scientific; #PA5-22281), anti-GAPDH Antibody [EPR16891] (Abcam; #ab181602), Monoclonal Antibody to Mouse Viperin (Hycult Biotech; #HM1016), anti-NPM1 Monoclonal Antibody FC-61991 (Thermo Fisher Scientific; #MA1-1560), and anti-POLR1A Antibody (Abcam; #ab222065).

    Techniques: Infection, Isolation, Western Blot, Software, Immunodetection, Positive Control, Transfection, Expressing, Plasmid Preparation