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elisa  (Hycult Biotech)


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    Hycult Biotech elisa
    Elisa, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HK504-IGG/EndoCAb+%2C+Human%2C+ELISA+kit/pmc12990172-62-18-23
    Average 95 stars, based on 61 article reviews
    elisa - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Mesenteric ischemia and bacterial translocation precipitate the intoxication phase of yellow fever
    Article Snippet: Quantification of microbial translocation-related plasma markers was performed using a commercially available Enzyme-linked Immunosorbent Assay (ELISA).

    Article Title: Early Combination Antiretroviral Therapy Initiation During Primary HIV Infection Restricts HIV Reservoirs and Gut-Driven Inflammation but Fails to Rewire the Systemic Cytokine Landscape
    Article Snippet: sCD14, EndoCab IgG Human, human lipolysaccharide binding protein LBP, human 1,3-beta-D-glucan, I-FABP and E-cadherin markers were measured by ELISA (EndoCab IgG cod HYC-HK504-IGG, Hycult Biotech, Uden, The Netherlands; LBP cod E0360HU-96; 1,3-beta-D-glucan cod E4111hu-96, BT LAB, Shanghai, China; Human sCD14 Immunoassay cod DC140; Human I-FABP Immunoassay cod DFBP20 and Human E-cadherin Immunoassay cod DCADE0B, R&D Systems, Minneapolis, MN, USA), following the manufacturer's instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Secretory IgA Is a Key Marker Among Gut Barrier Dysfunction‐Related Immunoglobulins Predicting Outcomes in ACLF
    Article Snippet: .. Commercially available ELISA kits were applied according to the manufacturer's instructions to determine the concentration of various general mucosal immune activation‐related and target‐specific IgA and IgG type anti‐microbial and auto‐antibodies according to the following: Secretory IgA (Abnova; Taipei, Taiwan; cat. #: KA3980); Free kappa and lambda IgLC (Biovendor; Brno, Czech Republic; cat. #: RD194088100R); EndoCab IgA (Hycult Biotech; Uden, Netherlands; cat. #: HK504‐IgA); Anti‐Gliadin IgA and IgG (INOVA Diagnostics; San Diego, CA, USA; cat. #: 708 655 and 708 650); Anti‐F‐actin IgA and IgG (INOVA Diagnostics; San Diego, CA, USA; cat. #: 704 500 and 708 785); ASCA IgA and IgG (GA Generic Assays GmbH; Dahlewitz, Germany; cat. #: 4006 and 4007); Anti‐GP2 IgA and IgG (GA Generic Assays GmbH; Dahlewitz, Germany; cat. #: 3750 and 3850). sCD163 and sCD206 levels were determined by commercially available solid‐phase enzyme‐linked immunoassays (IQProducts, Groningen, Netherlands, Cat.: IQP‐383; and Ray Biotech, Norcross, GA, USA, Cat.: ELH‐MMR respectively) using an ETI‐MAX 3000 machine. .. Presepsin levels were measured by a chemiluminescent PATHFAST presepsin analyser (Mitsubishi Chemical Medience Corporation, Tokyo, Japan).

    Article Title: Immune cell infiltration correlates with intestinal permeability, inflammation, and gastrointestinal symptoms in type 1 diabetes
    Article Snippet: Serum LPS activity was measured using a Hycult LAL chromogenic endpoint assay (HIT302, Hycult Biotech, Uden, The Netherlands). .. EndoCAb IgG and IgM were measured by Hycult EndoCAb IgG and IgM Elisa kits (HK504‐IGG; HK504‐IGM; Hycult Biotech, Uden, The Netherlands). .. LBP was measured using a Hycult LBP Human Elisa kit (HK315‐02, Hycult Biotech, Uden, The Netherlands).

    Article Title: Immune cell infiltration correlates with intestinal permeability, inflammation, and gastrointestinal symptoms in type 1 diabetes.
    Article Snippet: Serum LPS activity was measured using a Hycult LAL chromogenic endpoint assay (HIT302, Hycult Biotech, Uden, The Netherlands). .. EndoCAb IgG and IgM were measured by Hycult EndoCAb IgG and IgM Elisa kits (HK504-IGG; HK504-IGM; Hycult Biotech, Uden, The Netherlands). .. LBP was measured using a Hycult LBP Human Elisa kit (HK315-02, Hycult Biotech, Uden, The Netherlands).

    Concentration Assay:

    Article Title: Secretory IgA Is a Key Marker Among Gut Barrier Dysfunction‐Related Immunoglobulins Predicting Outcomes in ACLF
    Article Snippet: .. Commercially available ELISA kits were applied according to the manufacturer's instructions to determine the concentration of various general mucosal immune activation‐related and target‐specific IgA and IgG type anti‐microbial and auto‐antibodies according to the following: Secretory IgA (Abnova; Taipei, Taiwan; cat. #: KA3980); Free kappa and lambda IgLC (Biovendor; Brno, Czech Republic; cat. #: RD194088100R); EndoCab IgA (Hycult Biotech; Uden, Netherlands; cat. #: HK504‐IgA); Anti‐Gliadin IgA and IgG (INOVA Diagnostics; San Diego, CA, USA; cat. #: 708 655 and 708 650); Anti‐F‐actin IgA and IgG (INOVA Diagnostics; San Diego, CA, USA; cat. #: 704 500 and 708 785); ASCA IgA and IgG (GA Generic Assays GmbH; Dahlewitz, Germany; cat. #: 4006 and 4007); Anti‐GP2 IgA and IgG (GA Generic Assays GmbH; Dahlewitz, Germany; cat. #: 3750 and 3850). sCD163 and sCD206 levels were determined by commercially available solid‐phase enzyme‐linked immunoassays (IQProducts, Groningen, Netherlands, Cat.: IQP‐383; and Ray Biotech, Norcross, GA, USA, Cat.: ELH‐MMR respectively) using an ETI‐MAX 3000 machine. .. Presepsin levels were measured by a chemiluminescent PATHFAST presepsin analyser (Mitsubishi Chemical Medience Corporation, Tokyo, Japan).

    Activation Assay:

    Article Title: Secretory IgA Is a Key Marker Among Gut Barrier Dysfunction‐Related Immunoglobulins Predicting Outcomes in ACLF
    Article Snippet: .. Commercially available ELISA kits were applied according to the manufacturer's instructions to determine the concentration of various general mucosal immune activation‐related and target‐specific IgA and IgG type anti‐microbial and auto‐antibodies according to the following: Secretory IgA (Abnova; Taipei, Taiwan; cat. #: KA3980); Free kappa and lambda IgLC (Biovendor; Brno, Czech Republic; cat. #: RD194088100R); EndoCab IgA (Hycult Biotech; Uden, Netherlands; cat. #: HK504‐IgA); Anti‐Gliadin IgA and IgG (INOVA Diagnostics; San Diego, CA, USA; cat. #: 708 655 and 708 650); Anti‐F‐actin IgA and IgG (INOVA Diagnostics; San Diego, CA, USA; cat. #: 704 500 and 708 785); ASCA IgA and IgG (GA Generic Assays GmbH; Dahlewitz, Germany; cat. #: 4006 and 4007); Anti‐GP2 IgA and IgG (GA Generic Assays GmbH; Dahlewitz, Germany; cat. #: 3750 and 3850). sCD163 and sCD206 levels were determined by commercially available solid‐phase enzyme‐linked immunoassays (IQProducts, Groningen, Netherlands, Cat.: IQP‐383; and Ray Biotech, Norcross, GA, USA, Cat.: ELH‐MMR respectively) using an ETI‐MAX 3000 machine. .. Presepsin levels were measured by a chemiluminescent PATHFAST presepsin analyser (Mitsubishi Chemical Medience Corporation, Tokyo, Japan).

    Binding Assay:

    Article Title: Endotoxin Markers Are Elevated in Adolescents With Obesity With and Without Metabolic Dysfunction-Associated Steatotic Liver Disease.
    Article Snippet: Serum insulin was measured with an enzyme- linked immunosorbent assay (ELISA) from Alpco (product number #80- INSHU- CH01, Salem, NH). .. EndoCab IgG (#HK504- IgG) and lipopolysaccharide- binding protein (LBP, #HK315) were measured using ELISAs from Hycult Biotech (Wayne, PA). ..



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    (A) Abdominal organs after cavity opening in a 19-year old male YF victim showing enlarged liver with congestion and steatosis (yellow outline); dilated stomach with hemorrhagic wall (red arrow); hemorrhagic necrosis of the pancreas, with steatonecrosis in the retroperitoneal fat tissue (green arrow); dilated intestinal loops, with hemorrhagic walls (brown arrows). The abdominal cavity was filled with hemorrhagic fluid, mainly in the retroperitoneal area. (B) Ascending colon of a 47-year-old male YF victim showing confluent hemorrhagic spots (red arrow) and superficial ulcers on mucosa, associated with haemorrhages and steatonecrosis over the mesentery. The intestinal contents were hemorrhagic. (C) Hematoxylin and eosin stained small intestine from a YF victim showing epithelial denudation. (D,E) Detection of Gram-negative bacteria via LPS immunohistochemistry (IHC, red) in the portal vein and liver parenchyma of YF victims. Note the highly abnormal histological background due to YFV infection, showing degenerated hepatocytes and mixed tissue inflammatory reaction associated with Gram-negative bacteria. PV; portal vein. (F-J) Concentrations of 16s ribosomal DNA (F), Lipopolysaccharide binding <t>protein</t> <t>(LBP)</t> (G), soluble (s)CD14 (H), Endotoxin Core Antibody <t>(EndoCAb)</t> (I), and intestinal fatty acid binding protein (IFAB) (J), in samples collected at the hospital admission from patients who died from YF (n=27) or survived YF (n=63). As control groups, samples from survivors in the convalescent phase (Conv, n=16), YF vaccine (Vax’d, 14 days after vaccination) recipients (n=10) and healthy controls without recent vaccination (n=22) were used. Note, for the quantification of 16S rDNA, the number of healthy control samples tested (n=7) was lower due to the availability of samples. For all graphs, statistical significance was determined using the Kruskal-Wallis test with Dunn’s correction for multiple comparisons (*: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001). Only medically relevant comparisons are shown. Error bars show the median ± interquartile range.
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    (A) Abdominal organs after cavity opening in a 19-year old male YF victim showing enlarged liver with congestion and steatosis (yellow outline); dilated stomach with hemorrhagic wall (red arrow); hemorrhagic necrosis of the pancreas, with steatonecrosis in the retroperitoneal fat tissue (green arrow); dilated intestinal loops, with hemorrhagic walls (brown arrows). The abdominal cavity was filled with hemorrhagic fluid, mainly in the retroperitoneal area. (B) Ascending colon of a 47-year-old male YF victim showing confluent hemorrhagic spots (red arrow) and superficial ulcers on mucosa, associated with haemorrhages and steatonecrosis over the mesentery. The intestinal contents were hemorrhagic. (C) Hematoxylin and eosin stained small intestine from a YF victim showing epithelial denudation. (D,E) Detection of Gram-negative bacteria via LPS immunohistochemistry (IHC, red) in the portal vein and liver parenchyma of YF victims. Note the highly abnormal histological background due to YFV infection, showing degenerated hepatocytes and mixed tissue inflammatory reaction associated with Gram-negative bacteria. PV; portal vein. (F-J) Concentrations of 16s ribosomal DNA (F), Lipopolysaccharide binding <t>protein</t> <t>(LBP)</t> (G), soluble (s)CD14 (H), Endotoxin Core Antibody <t>(EndoCAb)</t> (I), and intestinal fatty acid binding protein (IFAB) (J), in samples collected at the hospital admission from patients who died from YF (n=27) or survived YF (n=63). As control groups, samples from survivors in the convalescent phase (Conv, n=16), YF vaccine (Vax’d, 14 days after vaccination) recipients (n=10) and healthy controls without recent vaccination (n=22) were used. Note, for the quantification of 16S rDNA, the number of healthy control samples tested (n=7) was lower due to the availability of samples. For all graphs, statistical significance was determined using the Kruskal-Wallis test with Dunn’s correction for multiple comparisons (*: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001). Only medically relevant comparisons are shown. Error bars show the median ± interquartile range.
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    Image Search Results


    (A) Abdominal organs after cavity opening in a 19-year old male YF victim showing enlarged liver with congestion and steatosis (yellow outline); dilated stomach with hemorrhagic wall (red arrow); hemorrhagic necrosis of the pancreas, with steatonecrosis in the retroperitoneal fat tissue (green arrow); dilated intestinal loops, with hemorrhagic walls (brown arrows). The abdominal cavity was filled with hemorrhagic fluid, mainly in the retroperitoneal area. (B) Ascending colon of a 47-year-old male YF victim showing confluent hemorrhagic spots (red arrow) and superficial ulcers on mucosa, associated with haemorrhages and steatonecrosis over the mesentery. The intestinal contents were hemorrhagic. (C) Hematoxylin and eosin stained small intestine from a YF victim showing epithelial denudation. (D,E) Detection of Gram-negative bacteria via LPS immunohistochemistry (IHC, red) in the portal vein and liver parenchyma of YF victims. Note the highly abnormal histological background due to YFV infection, showing degenerated hepatocytes and mixed tissue inflammatory reaction associated with Gram-negative bacteria. PV; portal vein. (F-J) Concentrations of 16s ribosomal DNA (F), Lipopolysaccharide binding protein (LBP) (G), soluble (s)CD14 (H), Endotoxin Core Antibody (EndoCAb) (I), and intestinal fatty acid binding protein (IFAB) (J), in samples collected at the hospital admission from patients who died from YF (n=27) or survived YF (n=63). As control groups, samples from survivors in the convalescent phase (Conv, n=16), YF vaccine (Vax’d, 14 days after vaccination) recipients (n=10) and healthy controls without recent vaccination (n=22) were used. Note, for the quantification of 16S rDNA, the number of healthy control samples tested (n=7) was lower due to the availability of samples. For all graphs, statistical significance was determined using the Kruskal-Wallis test with Dunn’s correction for multiple comparisons (*: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001). Only medically relevant comparisons are shown. Error bars show the median ± interquartile range.

    Journal: bioRxiv

    Article Title: Mesenteric ischemia and bacterial translocation precipitate the intoxication phase of yellow fever

    doi: 10.1101/2025.03.05.641677

    Figure Lengend Snippet: (A) Abdominal organs after cavity opening in a 19-year old male YF victim showing enlarged liver with congestion and steatosis (yellow outline); dilated stomach with hemorrhagic wall (red arrow); hemorrhagic necrosis of the pancreas, with steatonecrosis in the retroperitoneal fat tissue (green arrow); dilated intestinal loops, with hemorrhagic walls (brown arrows). The abdominal cavity was filled with hemorrhagic fluid, mainly in the retroperitoneal area. (B) Ascending colon of a 47-year-old male YF victim showing confluent hemorrhagic spots (red arrow) and superficial ulcers on mucosa, associated with haemorrhages and steatonecrosis over the mesentery. The intestinal contents were hemorrhagic. (C) Hematoxylin and eosin stained small intestine from a YF victim showing epithelial denudation. (D,E) Detection of Gram-negative bacteria via LPS immunohistochemistry (IHC, red) in the portal vein and liver parenchyma of YF victims. Note the highly abnormal histological background due to YFV infection, showing degenerated hepatocytes and mixed tissue inflammatory reaction associated with Gram-negative bacteria. PV; portal vein. (F-J) Concentrations of 16s ribosomal DNA (F), Lipopolysaccharide binding protein (LBP) (G), soluble (s)CD14 (H), Endotoxin Core Antibody (EndoCAb) (I), and intestinal fatty acid binding protein (IFAB) (J), in samples collected at the hospital admission from patients who died from YF (n=27) or survived YF (n=63). As control groups, samples from survivors in the convalescent phase (Conv, n=16), YF vaccine (Vax’d, 14 days after vaccination) recipients (n=10) and healthy controls without recent vaccination (n=22) were used. Note, for the quantification of 16S rDNA, the number of healthy control samples tested (n=7) was lower due to the availability of samples. For all graphs, statistical significance was determined using the Kruskal-Wallis test with Dunn’s correction for multiple comparisons (*: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001). Only medically relevant comparisons are shown. Error bars show the median ± interquartile range.

    Article Snippet: The following markers were used: I-FABP (Human I-FABP, Hycult Biotech, HK406), to assess enterocyte death (intestinal tissue damage) and sCD14 (Human sCD14 Quantikine ELISA kit, R&D Systems, DC140), LBP (Human LBP, Hycult Biotech, HK315) and EndoCAb (EndoCab IgM, Hycult Biotech, HK504-IgM) as indirect markers of microbial translocation.

    Techniques: Staining, Bacteria, Immunohistochemistry, Infection, Binding Assay, Control