Journal: Transplant International
Article Title: Activation of the Innate Immune System in Brain-Dead Donors Can Be Reduced by Luminal Intestinal Preservation During Organ Procurement Surgery - A Porcine Model
doi: 10.3389/ti.2024.13569
Figure Lengend Snippet: Procurement surgery-induced release of lipopolysaccharide binding protein in plasma was reduced by intestinal PEG intervention. Procurement surgery led to a significant increase in lipopolysaccharide binding protein (LBP) in all groups except the PEG intervention group (A) . At the end of organ procurement, lipopolysaccharide binding protein was significantly lower in the brain dead + PEG-group compared to all other groups (B) . Control: control group, Brain dead: brain dead without luminal intestinal intervention, Brain dead + PEG: brain dead with luminal intestinal intervention using polyethylene glycol, Brain dead + UW: brain dead with luminal intestinal intervention using University of Wisconsin solution, LBP: Lipopolysachharide binding protein. Values presented as mean ± 95% Confidence Interval. Mixed model. * p < 0.05.
Article Snippet: EDTA Plasma samples were analyzed for lipopolysaccharide binding protein (LBP) (Hycult Biotech, HK503, Uden, Netherlands), IL-8 (Merck & Co., PCYTMAG-23K, Rahway, NJ, United States), and TNF (R&D Systems, PTA00, Minneapolis, MN, United States) according to the manufacturer’s instructions.
Techniques: Binding Assay, Control