proteinase 3, human, elisa kit –  (Hycult Biotech)

Journal: Molecular Medicine

Article Title: Increased proteinase 3 and neutrophil elastase plasma concentrations are associated with non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes

doi: 10.1186/s10020-019-0084-3

Figure Lengend Snippet: PR3, NE, AAT and hsCRP plasma concentrations in patients with liver steatosis and type 2 diabetes versus lean and obese controls. a PR3 plasma concentrations in patients with liver steatosis and type 2 diabetes versus lean and obese controls. b NE plasma concentrations in patients with liver steatosis and type 2 diabetes versus lean and obese controls. c AAT plasma concentrations in patients with liver steatosis and type 2 diabetes versus lean and obese controls. d PR3 to AAT ratio in patients with liver steatosis and type 2 diabetes versus lean and obese controls. e NE to AAT ratio in patients with liver steatosis and type 2 diabetes versus lean and obese controls. f hsCRP concentrations in patients with liver steatosis and type 2 diabetes versus lean and obese controls. Data is represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, NS = p > 0.05

Article Snippet: PR3, NE and AAT plasma concentrations were quantified using sandwich ELISAs (enzyme-linked immunosorbent assay; cat# HK384, HK319 and HK387 respectively, Hycult Biotech, Uden, The Netherlands) according to manufacturer’s instructions.

Techniques:

Journal: Molecular Medicine

Article Title: Increased proteinase 3 and neutrophil elastase plasma concentrations are associated with non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes

doi: 10.1186/s10020-019-0084-3

Figure Lengend Snippet: PR3, NE and AAT plasma concentrations in patients with type 2 diabetes. a NE plasma concentrations in patients that didn't use insulin drugs versus patients that also used insulin. b NE plasma concentrations between patients with well controlled glycaemia versus patients with a poor control of glycaemia. c PR3 plasma concentrations in patients that didn't use insulin versus patients that also used insulin. d PR3 plasma concentrations between patients with well controlled glycaemia versus patients with a poor control of glycaemia. e AAT plasma concentrations in patients that didn't use insulin versus patients that also used insulin. f AAT plasma concentrations between patients with well controlled glycaemia versus patients with a poor control of glycaemia. Data is represented as mean ± SEM. ** p < 0.01, NS= p > 0.05

Article Snippet: PR3, NE and AAT plasma concentrations were quantified using sandwich ELISAs (enzyme-linked immunosorbent assay; cat# HK384, HK319 and HK387 respectively, Hycult Biotech, Uden, The Netherlands) according to manufacturer’s instructions.

Techniques:

Journal: The Journal of infectious diseases

Article Title: Circulating Neutrophil Extracellular Traps and Neutrophil Activation Are Increased in Proportion to Disease Severity in Human Malaria

doi: 10.1093/infdis/jiy661

Figure Lengend Snippet: Neutrophil activation in human malaria. Markers for neutrophil activation were quantified by enzyme linked immunosorbent assay in frozen plasma from malaria patients and controls. Markers included neutrophil elastase (A) and proteinase-3 (B), determined in 23 controls, 8 patients with severe Plasmodium falciparum (Pf), 46 uncomplicated malaria (UM) patients with Pf, 13 UM with Plasmodium malariae (Pm), and 36 UM with Plasmodium vivax (Pv). A third marker, myeloperoxidase (C), was measured in 23 controls, 7 patients with severe Pf, 47 with UM Pf, 14 with UM Pm, and 37 with UM Pv. Plots show median and individual data points in each group. The Kruskal-Wallis with Dunn’s multiple comparisons test was used for statistical comparisons to controls. Significant differences in all plots are signified by asterisks (***, P < .0005; ****, P < .0001). The Mann-Whitney test was used for comparison between UM and severe Pf (#, P < .0005; ##, P < .0001). Data are presented in Supplementary Table S2.

Article Snippet: Neutrophil Activation and Parasite Biomass ( Plasmodium falciparum Histidine-Rich Protein-2) Neutrophil elastase (NE) and proteinase-3 (PR3) concentrations were determined in platelet-free plasma using the Human Polymorphonuclear NE Enzyme Linked Immunosorbent Assay (ELISA) kit (Abcam, Cambridge, UK) and the PR3 Human ELISA kit (HycultBiotech, Uden, Netherlands), respectively.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Marker, MANN-WHITNEY

Journal: The Journal of infectious diseases

Article Title: Circulating Neutrophil Extracellular Traps and Neutrophil Activation Are Increased in Proportion to Disease Severity in Human Malaria

doi: 10.1093/infdis/jiy661

Figure Lengend Snippet: Neutrophil activation in malaria inpatients on admission and discharge from hospital. Paired data (connected dots) were available for 9 Plasmodium falciparum (Pf) and 3 Plasmodium vivax (Pv) patients who were admitted and had neutrophil elastase (A) and proteinase-3 (B) concentrations tested again before being discharged from hospital. The Wilcoxon test was used for statistical comparisons. Significant differences are signified by asterisks (*, P < .05; **, P < .005).

Article Snippet: Neutrophil Activation and Parasite Biomass ( Plasmodium falciparum Histidine-Rich Protein-2) Neutrophil elastase (NE) and proteinase-3 (PR3) concentrations were determined in platelet-free plasma using the Human Polymorphonuclear NE Enzyme Linked Immunosorbent Assay (ELISA) kit (Abcam, Cambridge, UK) and the PR3 Human ELISA kit (HycultBiotech, Uden, Netherlands), respectively.

Techniques: Activation Assay