hk325 human calprotectin elisa kit  (Hycult Biotech)

Journal: ERJ Open Research

Article Title: Neutrophil extracellular traps are associated with airways inflammation and increased severity of lung disease in cystic fibrosis

doi: 10.1183/23120541.00312-2024

Figure Lengend Snippet: Sputum properties in cystic fibrosis (CF). a) Sputum haematoxylin and eosin staining in healthy controls (HCs) demonstrates the predominance of macrophages (solid white arrows). Neutrophils are also present (solid black arrows). Other small populations are epithelial cells (open black arrows) and squamous cells (open white arrows). b) Neutrophils are the predominant cell in CF sputum (solid black arrows), with only occasional macrophages (solid white arrows) being observed. The presence of multiple vacuoles was noted in CF neutrophils. c) CF sputum contains greater numbers of neutrophils per g of sputum, suggesting that not only is there more sputum produced in CF, but it contains more neutrophils than healthy sputum, CF 2.9×10 7 (7×10 6 –7.7×10 7 ⋅g −1 ) and HCs 6.88×10 5 (2.32×10 5 –1.26×10 6 ⋅g −1 ) (median (95% CI), p<0.0001). d) The percentage of neutrophils containing vacuoles in CF is higher than HCs (p<0.0001). e) Sputum double-stranded DNA (dsDNA) is increased in CF sputum. f) Sputum calprotectin is significantly elevated in CF. ****: p<0.0001.

Article Snippet: Calprotectin detection antibody (biotinylated tracer antibody from HK379 Human Calprotectin ELISA kit, Hycult, Germany) was used in line with the manufacturer's instructions, including TMB reagent and Stop Solution.

Techniques: Staining, Produced

Journal: ERJ Open Research

Article Title: Neutrophil extracellular traps are associated with airways inflammation and increased severity of lung disease in cystic fibrosis

doi: 10.1183/23120541.00312-2024

Figure Lengend Snippet: Neutrophil extracellular traps (NETs) in CF. a) Double-stranded DNA (dsDNA) and calprotectin are highly correlated in cystic fibrosis (CF) sputum (Pearson r=0.7201, p<0.0001). b) Sputum NETs measured by histone/calprotectin ELISA are elevated in CF sputum compared with healthy controls (HCs) (p<0.0001). c) Sputum NET levels are not related to severity of disease measured by lung function. d) NETs were higher in people with CF not chronically colonised with Pseudomonas aeruginosa (PA) (p<0.05). e) Colonisation with Aspergillus (Asp) was not associated with the abundance of NETs. f) Frequent (>3 per year) and infrequent (<3 per year) exacerbators had no overall difference in sputum NET measurements. g) Waterfall plot of organisms recorded by clinical microbiology (standard culture) at the time of NET sample (on day or within 2 weeks). Generally, non-PA patients were segregated at the bottom of the graph with more NETs. Multi-resistant PA was not associated with greater NETs. SA: Staphylococcus aureus ; HI: Haemophilus influenzae ; PA: Pseudomonas aeruginosa ; SAL: Streptococcus agalactiae ; AX: Achromobacter xylosoxidans ; BM: Burkholderia multivorans ; BC: Burkholderia cepacia ; BCC: Burkholderia cenocepacia ; SEM: Serratia marcescens ; SM: Stenotrophomonas maltophilia ; PPn: Pandoraea pnomenusa ; Pr: proteus ; SP: Streptococcus pneumoniae ; E. coli : Escherichia coli ; FEV 1 : forced expiratory volume in 1 s. *: <0.05; ****: <0.0001; NS: not significant.

Article Snippet: Calprotectin detection antibody (biotinylated tracer antibody from HK379 Human Calprotectin ELISA kit, Hycult, Germany) was used in line with the manufacturer's instructions, including TMB reagent and Stop Solution.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: ERJ Open Research

Article Title: Neutrophil extracellular traps are associated with airways inflammation and increased severity of lung disease in cystic fibrosis

doi: 10.1183/23120541.00312-2024

Figure Lengend Snippet: Association of neutrophil extracellular traps (NETs) and inflammation. a) Correlation matrix of NETs and other sputum markers of inflammation. Calprotectin (Spearman r=0.548, p=0.001), interleukin (IL)-8 (Spearman r=0.419, p=0.004), myeloperoxidase (MPO) (Spearman r=0.428, p=0.003) and human neutrophil elastase (HNE) (Spearman r=0.338, p=0.02) activity were statistically correlated with NET levels. Please also see for additional mediator data. b) Patients taking azithromycin (Azithro) as an immunomodulator treatment do not have different levels of sputum NETs. c) DNase treatment is associated with fewer NETs (p<0.05). dsDNA: double-stranded DNA; TNF: tumour necrosis factor; FEV 1 : forced expiratory volume in 1 s. *: p<0.05; NS: not significant.

Article Snippet: Calprotectin detection antibody (biotinylated tracer antibody from HK379 Human Calprotectin ELISA kit, Hycult, Germany) was used in line with the manufacturer's instructions, including TMB reagent and Stop Solution.

Techniques: Activity Assay

Journal: Clinical and Translational Gastroenterology

Article Title: Noninvasive Markers of Inflammation and Protein Loss Augment Diagnosis of Pediatric Celiac Disease

doi: 10.14309/ctg.0000000000000695

Figure Lengend Snippet: Comparison of fecal and plasma markers in cases vs controls. P values from the Mann-Whitney test. ( a ) Plasma lipocalin-2 results, ( b ) fecal lipocalin-2 results, ( c ) fecal calprotectin results, ( d ) fecal A1AT, and ( e ) TTG IgA results. Horizontal lines represent medians. A1AT, alpha-1 antitrypsin; Ig, immunoglobulin; TTG, tissue transglutaminase.

Article Snippet: Fecal calprotectin concentrations were determined using a commercially available kit to perform a sandwich ELISA for human calprotectin (Human Calprotectin HycultBiotech, Wayne, PA).

Techniques: Comparison, MANN-WHITNEY

Journal: Clinical and Translational Gastroenterology

Article Title: Noninvasive Markers of Inflammation and Protein Loss Augment Diagnosis of Pediatric Celiac Disease

doi: 10.14309/ctg.0000000000000695

Figure Lengend Snippet: Comparison of fecal and plasma markers vs modified Marsh score. P values from the Kruskal-Wallis test. ( a ) Plasma lipocalin results, ( b ) fecal lipocalin-2 results, ( c ) fecal calprotectin results, and ( d ) fecal A1AT results. Horizontal lines represent medians. A1AT, alpha-1 antitrypsin.

Article Snippet: Fecal calprotectin concentrations were determined using a commercially available kit to perform a sandwich ELISA for human calprotectin (Human Calprotectin HycultBiotech, Wayne, PA).

Techniques: Comparison, Modification

Journal: Clinical and Translational Gastroenterology

Article Title: Noninvasive Markers of Inflammation and Protein Loss Augment Diagnosis of Pediatric Celiac Disease

doi: 10.14309/ctg.0000000000000695

Figure Lengend Snippet: Comparison of fecal and plasma markers vs normalized TTG. P values from linear regression. ( a ) Plasma lipocalin-2 results, ( b ) fecal lipocalin-2 results, ( c ) fecal calprotectin results, and ( d ) fecal A1AT results. A1AT, alpha-1 antitrypsin; TTG, tissue transglutaminase; ULN, upper limit of normal.

Article Snippet: Fecal calprotectin concentrations were determined using a commercially available kit to perform a sandwich ELISA for human calprotectin (Human Calprotectin HycultBiotech, Wayne, PA).

Techniques: Comparison