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c3 elisa kit  (Hycult Biotech)


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    Structured Review

    Hycult Biotech c3 elisa kit
    C3 Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    c3 elisa kit - by Bioz Stars, 2025-02
    94/100 stars

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    Hycult Biotech c3 elisa
    Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) <t>C3</t> mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); <t>ELISA</t> control standard curves and protein concentrations in the basal supernatants are shown in the .
    C3 Elisa, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); ELISA control standard curves and protein concentrations in the basal supernatants are shown in the .

    Journal: Antioxidants

    Article Title: Oxidative Stress Increases Endogenous Complement-Dependent Inflammatory and Angiogenic Responses in Retinal Pigment Epithelial Cells Independently of Exogenous Complement Sources

    doi: 10.3390/antiox8110548

    Figure Lengend Snippet: Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); ELISA control standard curves and protein concentrations in the basal supernatants are shown in the .

    Article Snippet: C3 quantification was performed using a newly developed C3 ELISA (cat# HK366-01; Hycult Biotech, Uden, Netherlands) according to the manufacturer’s protocol.

    Techniques: Immunohistochemistry, Protein Concentration, Standard Deviation, Enzyme-linked Immunosorbent Assay