quantitative human gc1qr elisa kit  (Hycult Biotech)


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    Hycult Biotech quantitative human gc1qr elisa kit
    Mesothelioma MSTO-211H cells expresses <t>gC1qR.</t> (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).
    Quantitative Human Gc1qr Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative human gc1qr elisa kit/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative human gc1qr elisa kit - by Bioz Stars, 2024-09
    90/100 stars

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    1) Product Images from "gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma"

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.01413

    Mesothelioma MSTO-211H cells expresses gC1qR. (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).
    Figure Legend Snippet: Mesothelioma MSTO-211H cells expresses gC1qR. (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).

    Techniques Used: Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing

    Mesothelioma cells shed gC1qR into the extracellular milieu. MSTO-211H cells were cultured in complete medium, and supernatants were sampled at 24-h intervals. Relative concentrations of gC1qR in the supernatants at each time point were determined via a direct ELISA. Error bars represent mean substrate absorbance ± standard deviation. n = 2 separate experiments performed in duplicate. *Significantly different results at p < 0.05.
    Figure Legend Snippet: Mesothelioma cells shed gC1qR into the extracellular milieu. MSTO-211H cells were cultured in complete medium, and supernatants were sampled at 24-h intervals. Relative concentrations of gC1qR in the supernatants at each time point were determined via a direct ELISA. Error bars represent mean substrate absorbance ± standard deviation. n = 2 separate experiments performed in duplicate. *Significantly different results at p < 0.05.

    Techniques Used: Cell Culture, Direct ELISA, Standard Deviation

    Extracellular gC1qR enhances MSTO-211H cell growth in culture. Tissue culture wells were coated with gC1qR or coating buffer (control) and seeded with MSTO-211H cells. Cells were supplemented with recombinant gC1qR (5 μg/ml) as noted. Cell cultures were imaged at 24-h intervals via compound light microscopy (10× magnification). Representative images are shown. Increased cell growth is evident at 72 and 96 h in the presence of soluble or immobilized extracellular gC1qR.
    Figure Legend Snippet: Extracellular gC1qR enhances MSTO-211H cell growth in culture. Tissue culture wells were coated with gC1qR or coating buffer (control) and seeded with MSTO-211H cells. Cells were supplemented with recombinant gC1qR (5 μg/ml) as noted. Cell cultures were imaged at 24-h intervals via compound light microscopy (10× magnification). Representative images are shown. Increased cell growth is evident at 72 and 96 h in the presence of soluble or immobilized extracellular gC1qR.

    Techniques Used: Recombinant, Light Microscopy

    Antibodies directed against the C1q binding site of gC1qR decrease mesothelioma cell proliferation. MSTO-211H cells was treated with 10 μg/mL monoclonal anti gC1qR antibody mAb 60.11 directed against the C1q binding region (aa 76–93) or mAb 74.5.2 directed against the high molecular weight kininogen binding domain (aa 204–218). Cell proliferation was determined via hemocytometer cell counts of viable cells after 96 h incubation. Error bars represent mean cell population ± standard deviation. n = 2 separate experiments performed in duplicate. *Significant difference from control at p = 1.33 × 10 −5 .
    Figure Legend Snippet: Antibodies directed against the C1q binding site of gC1qR decrease mesothelioma cell proliferation. MSTO-211H cells was treated with 10 μg/mL monoclonal anti gC1qR antibody mAb 60.11 directed against the C1q binding region (aa 76–93) or mAb 74.5.2 directed against the high molecular weight kininogen binding domain (aa 204–218). Cell proliferation was determined via hemocytometer cell counts of viable cells after 96 h incubation. Error bars represent mean cell population ± standard deviation. n = 2 separate experiments performed in duplicate. *Significant difference from control at p = 1.33 × 10 −5 .

    Techniques Used: Binding Assay, Molecular Weight, Incubation, Standard Deviation

    Targeted  gC1qR  (60.11) treatment reduces MSTO-211H tumor cell growth in an orthotopic murine xenotransplant model.
    Figure Legend Snippet: Targeted gC1qR (60.11) treatment reduces MSTO-211H tumor cell growth in an orthotopic murine xenotransplant model.

    Techniques Used:

    quantitative human gc1qr elisa kit  (Hycult Biotech)


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    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
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    Hycult Biotech quantitative human gc1qr elisa kit
    Mesothelioma MSTO-211H cells expresses <t>gC1qR.</t> (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).
    Quantitative Human Gc1qr Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative human gc1qr elisa kit/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative human gc1qr elisa kit - by Bioz Stars, 2024-09
    90/100 stars

    Images

    1) Product Images from "gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma"

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.01413

    Mesothelioma MSTO-211H cells expresses gC1qR. (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).
    Figure Legend Snippet: Mesothelioma MSTO-211H cells expresses gC1qR. (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).

    Techniques Used: Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing

    Mesothelioma cells shed gC1qR into the extracellular milieu. MSTO-211H cells were cultured in complete medium, and supernatants were sampled at 24-h intervals. Relative concentrations of gC1qR in the supernatants at each time point were determined via a direct ELISA. Error bars represent mean substrate absorbance ± standard deviation. n = 2 separate experiments performed in duplicate. *Significantly different results at p < 0.05.
    Figure Legend Snippet: Mesothelioma cells shed gC1qR into the extracellular milieu. MSTO-211H cells were cultured in complete medium, and supernatants were sampled at 24-h intervals. Relative concentrations of gC1qR in the supernatants at each time point were determined via a direct ELISA. Error bars represent mean substrate absorbance ± standard deviation. n = 2 separate experiments performed in duplicate. *Significantly different results at p < 0.05.

    Techniques Used: Cell Culture, Direct ELISA, Standard Deviation

    Extracellular gC1qR enhances MSTO-211H cell growth in culture. Tissue culture wells were coated with gC1qR or coating buffer (control) and seeded with MSTO-211H cells. Cells were supplemented with recombinant gC1qR (5 μg/ml) as noted. Cell cultures were imaged at 24-h intervals via compound light microscopy (10× magnification). Representative images are shown. Increased cell growth is evident at 72 and 96 h in the presence of soluble or immobilized extracellular gC1qR.
    Figure Legend Snippet: Extracellular gC1qR enhances MSTO-211H cell growth in culture. Tissue culture wells were coated with gC1qR or coating buffer (control) and seeded with MSTO-211H cells. Cells were supplemented with recombinant gC1qR (5 μg/ml) as noted. Cell cultures were imaged at 24-h intervals via compound light microscopy (10× magnification). Representative images are shown. Increased cell growth is evident at 72 and 96 h in the presence of soluble or immobilized extracellular gC1qR.

    Techniques Used: Recombinant, Light Microscopy

    Antibodies directed against the C1q binding site of gC1qR decrease mesothelioma cell proliferation. MSTO-211H cells was treated with 10 μg/mL monoclonal anti gC1qR antibody mAb 60.11 directed against the C1q binding region (aa 76–93) or mAb 74.5.2 directed against the high molecular weight kininogen binding domain (aa 204–218). Cell proliferation was determined via hemocytometer cell counts of viable cells after 96 h incubation. Error bars represent mean cell population ± standard deviation. n = 2 separate experiments performed in duplicate. *Significant difference from control at p = 1.33 × 10 −5 .
    Figure Legend Snippet: Antibodies directed against the C1q binding site of gC1qR decrease mesothelioma cell proliferation. MSTO-211H cells was treated with 10 μg/mL monoclonal anti gC1qR antibody mAb 60.11 directed against the C1q binding region (aa 76–93) or mAb 74.5.2 directed against the high molecular weight kininogen binding domain (aa 204–218). Cell proliferation was determined via hemocytometer cell counts of viable cells after 96 h incubation. Error bars represent mean cell population ± standard deviation. n = 2 separate experiments performed in duplicate. *Significant difference from control at p = 1.33 × 10 −5 .

    Techniques Used: Binding Assay, Molecular Weight, Incubation, Standard Deviation

    Targeted  gC1qR  (60.11) treatment reduces MSTO-211H tumor cell growth in an orthotopic murine xenotransplant model.
    Figure Legend Snippet: Targeted gC1qR (60.11) treatment reduces MSTO-211H tumor cell growth in an orthotopic murine xenotransplant model.

    Techniques Used:

    quantitative human gc1qr elisa kit  (Hycult Biotech)


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    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
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    Hycult Biotech quantitative human gc1qr elisa kit
    Quantitative Human Gc1qr Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative human gc1qr elisa kit/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative human gc1qr elisa kit - by Bioz Stars, 2024-09
    90/100 stars

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    Hycult Biotech quantitative human gc1qr elisa kit
    Mesothelioma MSTO-211H cells expresses <t>gC1qR.</t> (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).
    Quantitative Human Gc1qr Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative human gc1qr elisa kit/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative human gc1qr elisa kit - by Bioz Stars, 2024-09
    90/100 stars
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    Mesothelioma MSTO-211H cells expresses gC1qR. (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).

    Journal: Frontiers in Oncology

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    doi: 10.3389/fonc.2020.01413

    Figure Lengend Snippet: Mesothelioma MSTO-211H cells expresses gC1qR. (A) Immunofluorescence photomicrographs of stained MSTO-211H cells in culture. Results of a typical experiment. Non-permeabilized cell monolayers were stained with non-immune rabbit IgG (NIRG) or 60.11 anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488 (green), as well as DAPI nuclear stain (blue). Cells were imaged with a fluorescence microscope at 10× magnification. The presence of gC1qR is shown by green staining in the right image. (B) Flow cytometric analysis of MSTO-211H cell surface gC1qR expression. Non-permeabilized MSTO-211H cells in suspension were stained with non-immune mouse IgG (MOPC) or anti gC1qR antibody (āgC1qR) and appropriate secondary antibodies conjugated to AlexaFluor 488. Stained and unstained cells were analyzed. Expression of cell surface gC1qR in a typical experiment is shown by an increase in fluorescence of cells treated with anti gC1qR antibody (blue curve).

    Article Snippet: Soluble gC1qR in pleural fluid from patients with advanced malignant pleural mesothelioma was quantified using a commercial, quantitative human gC1qR ELISA kit (Hycult, Netherlands) ( ).

    Techniques: Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing

    Mesothelioma cells shed gC1qR into the extracellular milieu. MSTO-211H cells were cultured in complete medium, and supernatants were sampled at 24-h intervals. Relative concentrations of gC1qR in the supernatants at each time point were determined via a direct ELISA. Error bars represent mean substrate absorbance ± standard deviation. n = 2 separate experiments performed in duplicate. *Significantly different results at p < 0.05.

    Journal: Frontiers in Oncology

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    doi: 10.3389/fonc.2020.01413

    Figure Lengend Snippet: Mesothelioma cells shed gC1qR into the extracellular milieu. MSTO-211H cells were cultured in complete medium, and supernatants were sampled at 24-h intervals. Relative concentrations of gC1qR in the supernatants at each time point were determined via a direct ELISA. Error bars represent mean substrate absorbance ± standard deviation. n = 2 separate experiments performed in duplicate. *Significantly different results at p < 0.05.

    Article Snippet: Soluble gC1qR in pleural fluid from patients with advanced malignant pleural mesothelioma was quantified using a commercial, quantitative human gC1qR ELISA kit (Hycult, Netherlands) ( ).

    Techniques: Cell Culture, Direct ELISA, Standard Deviation

    Extracellular gC1qR enhances MSTO-211H cell growth in culture. Tissue culture wells were coated with gC1qR or coating buffer (control) and seeded with MSTO-211H cells. Cells were supplemented with recombinant gC1qR (5 μg/ml) as noted. Cell cultures were imaged at 24-h intervals via compound light microscopy (10× magnification). Representative images are shown. Increased cell growth is evident at 72 and 96 h in the presence of soluble or immobilized extracellular gC1qR.

    Journal: Frontiers in Oncology

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    doi: 10.3389/fonc.2020.01413

    Figure Lengend Snippet: Extracellular gC1qR enhances MSTO-211H cell growth in culture. Tissue culture wells were coated with gC1qR or coating buffer (control) and seeded with MSTO-211H cells. Cells were supplemented with recombinant gC1qR (5 μg/ml) as noted. Cell cultures were imaged at 24-h intervals via compound light microscopy (10× magnification). Representative images are shown. Increased cell growth is evident at 72 and 96 h in the presence of soluble or immobilized extracellular gC1qR.

    Article Snippet: Soluble gC1qR in pleural fluid from patients with advanced malignant pleural mesothelioma was quantified using a commercial, quantitative human gC1qR ELISA kit (Hycult, Netherlands) ( ).

    Techniques: Recombinant, Light Microscopy

    Antibodies directed against the C1q binding site of gC1qR decrease mesothelioma cell proliferation. MSTO-211H cells was treated with 10 μg/mL monoclonal anti gC1qR antibody mAb 60.11 directed against the C1q binding region (aa 76–93) or mAb 74.5.2 directed against the high molecular weight kininogen binding domain (aa 204–218). Cell proliferation was determined via hemocytometer cell counts of viable cells after 96 h incubation. Error bars represent mean cell population ± standard deviation. n = 2 separate experiments performed in duplicate. *Significant difference from control at p = 1.33 × 10 −5 .

    Journal: Frontiers in Oncology

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    doi: 10.3389/fonc.2020.01413

    Figure Lengend Snippet: Antibodies directed against the C1q binding site of gC1qR decrease mesothelioma cell proliferation. MSTO-211H cells was treated with 10 μg/mL monoclonal anti gC1qR antibody mAb 60.11 directed against the C1q binding region (aa 76–93) or mAb 74.5.2 directed against the high molecular weight kininogen binding domain (aa 204–218). Cell proliferation was determined via hemocytometer cell counts of viable cells after 96 h incubation. Error bars represent mean cell population ± standard deviation. n = 2 separate experiments performed in duplicate. *Significant difference from control at p = 1.33 × 10 −5 .

    Article Snippet: Soluble gC1qR in pleural fluid from patients with advanced malignant pleural mesothelioma was quantified using a commercial, quantitative human gC1qR ELISA kit (Hycult, Netherlands) ( ).

    Techniques: Binding Assay, Molecular Weight, Incubation, Standard Deviation

    Targeted  gC1qR  (60.11) treatment reduces MSTO-211H tumor cell growth in an orthotopic murine xenotransplant model.

    Journal: Frontiers in Oncology

    Article Title: gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma

    doi: 10.3389/fonc.2020.01413

    Figure Lengend Snippet: Targeted gC1qR (60.11) treatment reduces MSTO-211H tumor cell growth in an orthotopic murine xenotransplant model.

    Article Snippet: Soluble gC1qR in pleural fluid from patients with advanced malignant pleural mesothelioma was quantified using a commercial, quantitative human gC1qR ELISA kit (Hycult, Netherlands) ( ).

    Techniques: