c1q, human, elisa kit  (Hycult Biotech)

Journal: Muscle & nerve

Article Title: Visualization and characterization of complement activation in acetylcholine receptor antibody seropositive myasthenia gravis.

doi: 10.1002/mus.28227

Figure Lengend Snippet: FIGURE 1 Quantification of plasma and serum complement components in healthy controls (HC) and myasthenia gravis (MG) patients. Concentrations of complement components were assessed by ELISA and MBSI. Complement activation products were shown in (A) and (B); component components were shown in (C)–(H). All values were presented as median with 95% confidence interval. The bright triangles indicate seronegative patients. ***p < .001, ****p < .0001. MG, myasthenia gravis patients. Statistical significance was defined by the Benjamini–Hochberg method with threshold p values (.00625 for plasma C3a, .0125 for plasma sC5b-9, .01875 for plasma properdin, .025 for plasma Factor I, .03125 for plasma C5, and .00625 for serum Factor I). ELISA, enzyme-linked immunosorbent assay; MBSI, magnetic bead-based sandwich immunoassay.

Article Snippet: C1q concentrations in individual samples from MG patients and HCs were measured by a commercial enzyme-linked immunosorbent assay (ELISA) kit (HK356, Hycult Biotech).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: iScience

Article Title: sMR and PTX3 levels associate with COVID-19 outcome and survival but not with Long COVID

doi: 10.1016/j.isci.2024.110162

Figure Lengend Snippet:

Article Snippet: Q1q, Human, ELISA Kit , Hycult Biotech , Cat# HK356.

Techniques: Enzyme-linked Immunosorbent Assay, Software, Luminex

Journal: iScience

Article Title: sMR and PTX3 levels associate with COVID-19 outcome and survival but not with Long COVID

doi: 10.1016/j.isci.2024.110162

Figure Lengend Snippet: Descriptive statistics of the patient and control cohorts

Article Snippet: Plasma concentrations for the markers PTX3, C1q, C1-INH, C1s/C1-INH complex and sMR were measured in EDTA-plasma using commercially available ELISA kits (Cat#: HK347 (PTX3), HK356 (C1q), HK396 (C1-INH), HK399 (C1s/C1-INH), and HK381 (sMR), Hycult Biotech, Uden, The Netherlands).

Techniques: Control, Sampling

Journal: iScience

Article Title: sMR and PTX3 levels associate with COVID-19 outcome and survival but not with Long COVID

doi: 10.1016/j.isci.2024.110162

Figure Lengend Snippet: Biomarker levels in moderate vs. severe COVID-19 cases and in severe non-ICU vs. ICU patients

Article Snippet: Plasma concentrations for the markers PTX3, C1q, C1-INH, C1s/C1-INH complex and sMR were measured in EDTA-plasma using commercially available ELISA kits (Cat#: HK347 (PTX3), HK356 (C1q), HK396 (C1-INH), HK399 (C1s/C1-INH), and HK381 (sMR), Hycult Biotech, Uden, The Netherlands).

Techniques: Biomarker Assay

Journal: iScience

Article Title: sMR and PTX3 levels associate with COVID-19 outcome and survival but not with Long COVID

doi: 10.1016/j.isci.2024.110162

Figure Lengend Snippet: Biomarkers characteristics, cut-off determination and ranking according to the Youden’s index (J)

Article Snippet: Plasma concentrations for the markers PTX3, C1q, C1-INH, C1s/C1-INH complex and sMR were measured in EDTA-plasma using commercially available ELISA kits (Cat#: HK347 (PTX3), HK356 (C1q), HK396 (C1-INH), HK399 (C1s/C1-INH), and HK381 (sMR), Hycult Biotech, Uden, The Netherlands).

Techniques: Comparison

Journal: iScience

Article Title: sMR and PTX3 levels associate with COVID-19 outcome and survival but not with Long COVID

doi: 10.1016/j.isci.2024.110162

Figure Lengend Snippet:

Article Snippet: Plasma concentrations for the markers PTX3, C1q, C1-INH, C1s/C1-INH complex and sMR were measured in EDTA-plasma using commercially available ELISA kits (Cat#: HK347 (PTX3), HK356 (C1q), HK396 (C1-INH), HK399 (C1s/C1-INH), and HK381 (sMR), Hycult Biotech, Uden, The Netherlands).

Techniques: Enzyme-linked Immunosorbent Assay, Software, Luminex

Journal: Muscle & nerve

Article Title: Activation of the classical complement pathway in myasthenia gravis with acetylcholine receptor antibodies.

doi: 10.1002/mus.27973

Figure Lengend Snippet: FIGURE 1 Serum levels of C1q, C5, C5a, soluble C5b-9 (sC5b-9), Ba, and complement factor H (CFH) in patients with myasthenia gravis (MG) and control subjects (CON). *Statistically significant after multiple-comparison adjustment (corrected significant p value using Bonferroni correction 0.05 / 6 = 0.008).

Article Snippet: Serum C1q, C5, and C5a levels were determined using human enzyme-linked immunosorbent assay kits for C1q (Catalog No. HK356), C5 (Catalog No. HK390), and C5a (Catalog No. HK349), respectively (Hycult Biotech, Uden, The Netherlands).

Techniques: Control, Comparison

Journal: Muscle & nerve

Article Title: Activation of the classical complement pathway in myasthenia gravis with acetylcholine receptor antibodies.

doi: 10.1002/mus.27973

Figure Lengend Snippet: FIGURE 2 Changes in baseline anti–acetylcholine receptor (AChR) antibody titers, scores of Myasthenia Gravis Activities of Daily Living (MGADL) score, and levels of C1q, C5, C5a, sC5b-9, Ba, and complement factor H (CFH) levels in 10 patients with myasthenia gravis about 6 months (range, 172 to 209 days) after immunotherapy. *Statistically significant after multiple-comparison adjustment (corrected significant p value using Bonferroni correction 0.05 / 8 = 0.006).

Article Snippet: Serum C1q, C5, and C5a levels were determined using human enzyme-linked immunosorbent assay kits for C1q (Catalog No. HK356), C5 (Catalog No. HK390), and C5a (Catalog No. HK349), respectively (Hycult Biotech, Uden, The Netherlands).

Techniques: Comparison