strem-1, human, elisa kit  (Hycult Biotech)

Journal: Scientific Reports

Article Title: Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay

doi: 10.1038/srep15381

Figure Lengend Snippet: Comparison of curve progression of rh-sTREM-1 standard in sTREM-1 Radsak ( A ), R&D ( B ), R&D + pcb ( C ) and Hycult Biotech ( D ) ELISA. 2,000 pg/mL of appropriate rh-sTREM-1 was employed and further twofold serially diluted for each ELISA. The OD was measured and depicted as the mean value of duplicates. One representative experiment out of three repeats is shown. The curves follow a four-parametric nonlinear curve fitting.

Article Snippet: Human sTREM-1 ELISA (HK348) was performed according to manufacturer’s instruction using pre-coated plates (Hycult Biotech, Uden, Netherlands).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay

doi: 10.1038/srep15381

Figure Lengend Snippet: Radsak ( A ), R&D ( B ), R&D + pcb ( C ) and Hycult Biotech ( D ) sTREM-1 ELISA were investigated for recovery of 2,000 pg/mL rh-sTREM-1 and twofold dilution series. Data are expressed as mean and SD conducted in duplicates and three independent experiments. Values ranging beyond the respective reference curve were set to zero for graph creation and excluded of further calculations or marked OVER.

Article Snippet: Human sTREM-1 ELISA (HK348) was performed according to manufacturer’s instruction using pre-coated plates (Hycult Biotech, Uden, Netherlands).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay

doi: 10.1038/srep15381

Figure Lengend Snippet: NHI and HI sera were diluted 1:4 and 2,000 pg/mL rh-sTREM-1 was added. Twofold serially dilution was performed and rh-sTREM-1 concentration was measured by the indicated ELISA. Mean and SD for three independent experiments each conducted in duplicates are shown. Values ranging beyond the designed standard curve were set to zero for graph creation and excluded of further calculations. Data were statistical tested for significant differences of rh-sTREM-1 of indicated concentrations in NHI and HI serum by two-tailed Wilcoxon matched-pairs test, followed by Bonferroni correction for multiple testing. *p < 0.05, **p < 0.01.

Article Snippet: Human sTREM-1 ELISA (HK348) was performed according to manufacturer’s instruction using pre-coated plates (Hycult Biotech, Uden, Netherlands).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Scientific Reports

Article Title: Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay

doi: 10.1038/srep15381

Figure Lengend Snippet: NHI and HI sera were 1:4 diluted and 4 U of reconstituted guinea pig complement (C’) was added to 240 μL-diluted sera. HI serum after addition of C’ was again heat-inactivated at 56 °C for 30 min to inactivate the complement. 2,000 pg/mL rh-sTREM-1 was added to each preparation and twofold serially dilution was performed. Shown are the results of extrapolated rh-sTREM-1 at 1,000 pg/mL tested by Radsak sTREM-1 ELISA. Mean and SD for three independent experiments each conducted in duplicates are shown. Statistical significance for indicated samples was calculated by two-tailed Wilcoxon matched-pairs test followed by Bonferroni correction for multiple testing. **p < 0.01.

Article Snippet: Human sTREM-1 ELISA (HK348) was performed according to manufacturer’s instruction using pre-coated plates (Hycult Biotech, Uden, Netherlands).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test