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hk337 madcam 1 elisa kit  (Hycult Biotech)


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    Structured Review

    Hycult Biotech hk337 madcam 1 elisa kit
    Endocervical and vaginal tissues were collected 5 weeks after the initiation of the hormones treatment and RT-qPCR for <t>MAdCAM-1,</t> CCL21, CCR5 and CCL4 was performed using a SybrGreen relative quantification assay. The expression of each gene was calculated relative to one animal chosen for each tissue. The graphs show the fold increase compared to the mean expression of the of the control group (bars represent mean±SEM). p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.
    Hk337 Madcam 1 Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hk337 madcam 1 elisa kit/product/Hycult Biotech
    Average 91 stars, based on 4 article reviews
    hk337 madcam 1 elisa kit - by Bioz Stars, 2026-02
    91/100 stars

    Images

    1) Product Images from "Sex Hormones Selectively Impact the Endocervical Mucosal Microenvironment: Implications for HIV Transmission"

    Article Title: Sex Hormones Selectively Impact the Endocervical Mucosal Microenvironment: Implications for HIV Transmission

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0097767

    Endocervical and vaginal tissues were collected 5 weeks after the initiation of the hormones treatment and RT-qPCR for MAdCAM-1, CCL21, CCR5 and CCL4 was performed using a SybrGreen relative quantification assay. The expression of each gene was calculated relative to one animal chosen for each tissue. The graphs show the fold increase compared to the mean expression of the of the control group (bars represent mean±SEM). p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.
    Figure Legend Snippet: Endocervical and vaginal tissues were collected 5 weeks after the initiation of the hormones treatment and RT-qPCR for MAdCAM-1, CCL21, CCR5 and CCL4 was performed using a SybrGreen relative quantification assay. The expression of each gene was calculated relative to one animal chosen for each tissue. The graphs show the fold increase compared to the mean expression of the of the control group (bars represent mean±SEM). p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.

    Techniques Used: Quantitative RT-PCR, Expressing

    The concentration of various cytokines and chemokines was measured in plasma of the differently treated (or untreated) animals by 29-Plex Luminex technology and by ELISA (MadCAM-1). Because of technical errors generated by the Luminex machine, we could not measure: CCL5 in 2 DMPA-, 1 E2- and 1 untreated animals; IFNγ in 1 DMPA-treated and 2 untreated animals. Bars represent mean±SEM. p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.
    Figure Legend Snippet: The concentration of various cytokines and chemokines was measured in plasma of the differently treated (or untreated) animals by 29-Plex Luminex technology and by ELISA (MadCAM-1). Because of technical errors generated by the Luminex machine, we could not measure: CCL5 in 2 DMPA-, 1 E2- and 1 untreated animals; IFNγ in 1 DMPA-treated and 2 untreated animals. Bars represent mean±SEM. p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.

    Techniques Used: Concentration Assay, Luminex, Enzyme-linked Immunosorbent Assay, Generated



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    Image Search Results


    Endocervical and vaginal tissues were collected 5 weeks after the initiation of the hormones treatment and RT-qPCR for MAdCAM-1, CCL21, CCR5 and CCL4 was performed using a SybrGreen relative quantification assay. The expression of each gene was calculated relative to one animal chosen for each tissue. The graphs show the fold increase compared to the mean expression of the of the control group (bars represent mean±SEM). p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.

    Journal: PLoS ONE

    Article Title: Sex Hormones Selectively Impact the Endocervical Mucosal Microenvironment: Implications for HIV Transmission

    doi: 10.1371/journal.pone.0097767

    Figure Lengend Snippet: Endocervical and vaginal tissues were collected 5 weeks after the initiation of the hormones treatment and RT-qPCR for MAdCAM-1, CCL21, CCR5 and CCL4 was performed using a SybrGreen relative quantification assay. The expression of each gene was calculated relative to one animal chosen for each tissue. The graphs show the fold increase compared to the mean expression of the of the control group (bars represent mean±SEM). p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.

    Article Snippet: The levels of sMAdCAM-1 were measured using the HK337 MAdCAM-1 ELISA kit (Hycult Biotech; detection level 0.4 ng/ml), in plasma (dilution 1∶10) and clarified vaginal swabs (dilution 1∶5) according to the manufacturer's protocol.

    Techniques: Quantitative RT-PCR, Expressing

    The concentration of various cytokines and chemokines was measured in plasma of the differently treated (or untreated) animals by 29-Plex Luminex technology and by ELISA (MadCAM-1). Because of technical errors generated by the Luminex machine, we could not measure: CCL5 in 2 DMPA-, 1 E2- and 1 untreated animals; IFNγ in 1 DMPA-treated and 2 untreated animals. Bars represent mean±SEM. p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.

    Journal: PLoS ONE

    Article Title: Sex Hormones Selectively Impact the Endocervical Mucosal Microenvironment: Implications for HIV Transmission

    doi: 10.1371/journal.pone.0097767

    Figure Lengend Snippet: The concentration of various cytokines and chemokines was measured in plasma of the differently treated (or untreated) animals by 29-Plex Luminex technology and by ELISA (MadCAM-1). Because of technical errors generated by the Luminex machine, we could not measure: CCL5 in 2 DMPA-, 1 E2- and 1 untreated animals; IFNγ in 1 DMPA-treated and 2 untreated animals. Bars represent mean±SEM. p<0.05 is considered significant. All the results with p<0.125 are also shown to indicate a tendency toward a significant difference.

    Article Snippet: The levels of sMAdCAM-1 were measured using the HK337 MAdCAM-1 ELISA kit (Hycult Biotech; detection level 0.4 ng/ml), in plasma (dilution 1∶10) and clarified vaginal swabs (dilution 1∶5) according to the manufacturer's protocol.

    Techniques: Concentration Assay, Luminex, Enzyme-linked Immunosorbent Assay, Generated