c3b, mouse, elisa kit  (Hycult Biotech)


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    Hycult Biotech c3b, mouse, elisa kit
    C3b, Mouse, Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c3b, mouse, elisa kit  (Hycult Biotech)


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    Hycult Biotech c3b, mouse, elisa kit
    C3b, Mouse, Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse c3b elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse c3b elisa kit
    Attenuation of IR-induced systemic complement activation products <t>C3b</t> and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by <t>ELISA.</t> Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).
    Mouse C3b Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    mouse c3b elisa kit - by Bioz Stars, 2024-05
    93/100 stars

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    1) Product Images from "A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia–reperfusion injury"

    Article Title: A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia–reperfusion injury

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-01423-y

    Attenuation of IR-induced systemic complement activation products C3b and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by ELISA. Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).
    Figure Legend Snippet: Attenuation of IR-induced systemic complement activation products C3b and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by ELISA. Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).

    Techniques Used: Activation Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    mouse c3b elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse c3b elisa kit
    Schematic representation of N- and C-terminal soluble recombinant truncation mutants of HuCR1. LHR domain boundaries are based on and from UniProt P17927 ( https://www.uniprot.org ). The green boxes denoted SP indicate the endogenous signal peptide, and red boxes denoted CP indicate the 19aa signal peptide from ceruloplasmin (GenBank accession number: NP_000087). Vertical bars with numbers above HuCR1(1971) denote the amino acid position of N-glycosylation sites. Amino acid numbering is based on Met + 1. Numbers below each construct indicate the amino acid position of the C-terminal end of the LHR domain boundaries, with the exception being at the N-terminal end of the mature sequence where the numbering denotes the start of the respective domain. Underneath the schematic is shown the known LHR domain(s) within CR1 responsible for both <t>C3b/C4b</t> ligand binding, decay acceleration activity, and cofactor activity, based on . HuCR1, human complement receptor 1; LHR, long homologous repeat.
    Mouse C3b Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A novel soluble complement receptor 1 fragment with enhanced therapeutic potential"

    Article Title: A novel soluble complement receptor 1 fragment with enhanced therapeutic potential

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA120.016127

    Schematic representation of N- and C-terminal soluble recombinant truncation mutants of HuCR1. LHR domain boundaries are based on and from UniProt P17927 ( https://www.uniprot.org ). The green boxes denoted SP indicate the endogenous signal peptide, and red boxes denoted CP indicate the 19aa signal peptide from ceruloplasmin (GenBank accession number: NP_000087). Vertical bars with numbers above HuCR1(1971) denote the amino acid position of N-glycosylation sites. Amino acid numbering is based on Met + 1. Numbers below each construct indicate the amino acid position of the C-terminal end of the LHR domain boundaries, with the exception being at the N-terminal end of the mature sequence where the numbering denotes the start of the respective domain. Underneath the schematic is shown the known LHR domain(s) within CR1 responsible for both C3b/C4b ligand binding, decay acceleration activity, and cofactor activity, based on . HuCR1, human complement receptor 1; LHR, long homologous repeat.
    Figure Legend Snippet: Schematic representation of N- and C-terminal soluble recombinant truncation mutants of HuCR1. LHR domain boundaries are based on and from UniProt P17927 ( https://www.uniprot.org ). The green boxes denoted SP indicate the endogenous signal peptide, and red boxes denoted CP indicate the 19aa signal peptide from ceruloplasmin (GenBank accession number: NP_000087). Vertical bars with numbers above HuCR1(1971) denote the amino acid position of N-glycosylation sites. Amino acid numbering is based on Met + 1. Numbers below each construct indicate the amino acid position of the C-terminal end of the LHR domain boundaries, with the exception being at the N-terminal end of the mature sequence where the numbering denotes the start of the respective domain. Underneath the schematic is shown the known LHR domain(s) within CR1 responsible for both C3b/C4b ligand binding, decay acceleration activity, and cofactor activity, based on . HuCR1, human complement receptor 1; LHR, long homologous repeat.

    Techniques Used: Recombinant, Construct, Sequencing, Ligand Binding Assay, Activity Assay

    Comparative affinity of CSL040 and CR1(1971) to human C3b and C4b. Biosensor data of plasma-derived human C3b binding to ( A ) CSL040 and ( B ) HuCR1(1971). Panels show double-referenced sensorgrams from a series of seven analyte concentrations (15.6, 31.3, 62.5, 125, 250, 500, and 1000 nM), injected for 150 s with dissociation monitored for 180 s. Each red line represents two overlaid sensorgrams. All C3b data were fit to a kinetic 1:1 model ( black lines ) including a term for mass transport. Calculated kinetic data are shown in <xref ref-type=Table 2 . Biosensor data of plasma-derived human C4b binding to ( C ) CSL040 and ( D ) HuCR1(1971). Panels show double-referenced sensorgrams from a series of five analyte concentrations (12.5, 25, 50, 100, and 200 nM) in twofold dilutions, injected for 120 s with dissociation monitored for 180 s. Each double red line represents two overlaid sensorgrams of same concentrations. C4b binding data cannot be fitted to a 1:1 model because of its biphasic binding nature. HuCR1, human complement receptor 1. " title="Comparative affinity of CSL040 and CR1(1971) to human C3b and C4b. Biosensor data of plasma-derived human C3b ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Comparative affinity of CSL040 and CR1(1971) to human C3b and C4b. Biosensor data of plasma-derived human C3b binding to ( A ) CSL040 and ( B ) HuCR1(1971). Panels show double-referenced sensorgrams from a series of seven analyte concentrations (15.6, 31.3, 62.5, 125, 250, 500, and 1000 nM), injected for 150 s with dissociation monitored for 180 s. Each red line represents two overlaid sensorgrams. All C3b data were fit to a kinetic 1:1 model ( black lines ) including a term for mass transport. Calculated kinetic data are shown in Table 2 . Biosensor data of plasma-derived human C4b binding to ( C ) CSL040 and ( D ) HuCR1(1971). Panels show double-referenced sensorgrams from a series of five analyte concentrations (12.5, 25, 50, 100, and 200 nM) in twofold dilutions, injected for 120 s with dissociation monitored for 180 s. Each double red line represents two overlaid sensorgrams of same concentrations. C4b binding data cannot be fitted to a 1:1 model because of its biphasic binding nature. HuCR1, human complement receptor 1.

    Techniques Used: Derivative Assay, Binding Assay, Injection

    Kinetic rate constants and affinity of plasma-derived human  C3b  and C4b binding to CSL040 or HuCR1(1971)
    Figure Legend Snippet: Kinetic rate constants and affinity of plasma-derived human C3b and C4b binding to CSL040 or HuCR1(1971)

    Techniques Used: Binding Assay

    The effect of CSL040 in an attenuated-passive anti-GBM glomerulonephritis mouse model. Glomerulonephritis was induced in C57BL/6 mice by i.v. injection of rabbit anti-GBM polyclonal antibody followed 6 days later by i.p. administration of MsαRb antibody. CSL040 ( filled circles ) or a PBS control ( open circles ) was given by i.p. administration 1 h prior to MsαRb. Naive C57BL/6 mice ( open triangles ) were used as an additional control. Urine was collected using metabolic cages, and mice were sacrificed after 24 h (for albuminuria and complement deposition) or 1 to 24 h (neutrophils staining in kidneys and measurement of plasma complement components). N = 6 to 8 per group. A , urine albumin levels in mice treated with PBS or CSL040 at 5, 20, or 60 mg/kg. B , intraglomerular infiltration of neutrophils 3 h after MsαRb administration was diminished by 60 mg/kg CSL040 as shown by the representative images of immunofluorescence staining of mouse kidneys (magnification 100×; the bar represents 100 μm). C , quantification of raw integrated density. D , infiltrating neutrophils in kidney were quantified by flow cytometry using the Ly6G and CD11b markers. The number next to the gate ( outlined with a box ) is neutrophils as a percentage of CD45 + cells in the plot of one representative mouse. E , neutrophil numbers from mice in each group were calculated from flow cytometry. F , effect of PBS or CSL040 (60 mg/kg) treatment on the plasma levels of complement activation fragment C3b/C3c/iC3b after induction of glomerulonephritis. Data shown are mean ± SD. Statistically significant differences in values between groups were calculated using ordinary one-way ANOVA with the Tukey's test for multiple comparisons. GBM, glomerular basement membrane; GN, glomerulonephritis.
    Figure Legend Snippet: The effect of CSL040 in an attenuated-passive anti-GBM glomerulonephritis mouse model. Glomerulonephritis was induced in C57BL/6 mice by i.v. injection of rabbit anti-GBM polyclonal antibody followed 6 days later by i.p. administration of MsαRb antibody. CSL040 ( filled circles ) or a PBS control ( open circles ) was given by i.p. administration 1 h prior to MsαRb. Naive C57BL/6 mice ( open triangles ) were used as an additional control. Urine was collected using metabolic cages, and mice were sacrificed after 24 h (for albuminuria and complement deposition) or 1 to 24 h (neutrophils staining in kidneys and measurement of plasma complement components). N = 6 to 8 per group. A , urine albumin levels in mice treated with PBS or CSL040 at 5, 20, or 60 mg/kg. B , intraglomerular infiltration of neutrophils 3 h after MsαRb administration was diminished by 60 mg/kg CSL040 as shown by the representative images of immunofluorescence staining of mouse kidneys (magnification 100×; the bar represents 100 μm). C , quantification of raw integrated density. D , infiltrating neutrophils in kidney were quantified by flow cytometry using the Ly6G and CD11b markers. The number next to the gate ( outlined with a box ) is neutrophils as a percentage of CD45 + cells in the plot of one representative mouse. E , neutrophil numbers from mice in each group were calculated from flow cytometry. F , effect of PBS or CSL040 (60 mg/kg) treatment on the plasma levels of complement activation fragment C3b/C3c/iC3b after induction of glomerulonephritis. Data shown are mean ± SD. Statistically significant differences in values between groups were calculated using ordinary one-way ANOVA with the Tukey's test for multiple comparisons. GBM, glomerular basement membrane; GN, glomerulonephritis.

    Techniques Used: Injection, Staining, Immunofluorescence, Flow Cytometry, Activation Assay

    Involvement of CR1/CSL040 in complement pathway inhibition. The three pathways of the complement systems are the classical, lectin, and alternative pathways, each activated by specific mechanisms. All pathways converge at the level of C3 before diverging to form the key complement end products: the C3a and C5a anaphylatoxins that mediate inflammatory processes; the opsonin C3b; and the lytic membrane attack complex. The activated fragments of C3 and C4, C3b and C4b, are key components in all three complement pathways, and CR1/CSL040 ( red boxes ) can bind both C3b and C4b, thereby inhibiting further complement activation. One mechanism is via CR1/CSL040 displacement of C2a or Bb in the C3 and C5 convertases (decay acceleration); the other is promotion of factor I-mediated cleavage of C3b/C4b into inactive fragments (cofactor activity). CR1, complement receptor 1.
    Figure Legend Snippet: Involvement of CR1/CSL040 in complement pathway inhibition. The three pathways of the complement systems are the classical, lectin, and alternative pathways, each activated by specific mechanisms. All pathways converge at the level of C3 before diverging to form the key complement end products: the C3a and C5a anaphylatoxins that mediate inflammatory processes; the opsonin C3b; and the lytic membrane attack complex. The activated fragments of C3 and C4, C3b and C4b, are key components in all three complement pathways, and CR1/CSL040 ( red boxes ) can bind both C3b and C4b, thereby inhibiting further complement activation. One mechanism is via CR1/CSL040 displacement of C2a or Bb in the C3 and C5 convertases (decay acceleration); the other is promotion of factor I-mediated cleavage of C3b/C4b into inactive fragments (cofactor activity). CR1, complement receptor 1.

    Techniques Used: Inhibition, Activation Assay, Activity Assay

    mouse c3b  (Hycult Biotech)


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    Hycult Biotech mouse c3b
    Mouse C3b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse c3b  (Hycult Biotech)


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    Hycult Biotech mouse c3b
    C5 blockade reduced renal IR-mediated complement activation and deposition. Twenty-four hours after reperfusion, kidney sections were analyzed for deposition of <t>complement</t> <t>C3b/c</t> (A) and C9 (C) using immunofluorescence staining/confocal microscopy, and fluorescence intensities were measured using Image J software (B and D). Nuclei were stained with DAPI. Tubular and glomerular epithelial and vascular endothelial C3b/c deposition (A) and tubular C9 deposition (C) were observed in isotype-treated mice. Treatment with BB5.1 reduced deposition of C3b/c (A and B) and C9 (C and D) compared with isotype control treatment. The data shown are mean ± SEM (n = 7–8). Scale bar, 50 μm. Statistical analysis was carried out using the Mann-Whitney U test ( ### P < 0.001 for sham vs isotype; ** P < 0.01 for isotype vs BB5.1). IR, ischemia-reperfusion; IRI, IR injury; RawIntDen, raw integrated density; SEM, standard error of the mean.
    Mouse C3b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Complement-mediated Damage to the Glycocalyx Plays a Role in Renal Ischemia-reperfusion Injury in Mice"

    Article Title: Complement-mediated Damage to the Glycocalyx Plays a Role in Renal Ischemia-reperfusion Injury in Mice

    Journal: Transplantation Direct

    doi: 10.1097/TXD.0000000000000881

    C5 blockade reduced renal IR-mediated complement activation and deposition. Twenty-four hours after reperfusion, kidney sections were analyzed for deposition of complement C3b/c (A) and C9 (C) using immunofluorescence staining/confocal microscopy, and fluorescence intensities were measured using Image J software (B and D). Nuclei were stained with DAPI. Tubular and glomerular epithelial and vascular endothelial C3b/c deposition (A) and tubular C9 deposition (C) were observed in isotype-treated mice. Treatment with BB5.1 reduced deposition of C3b/c (A and B) and C9 (C and D) compared with isotype control treatment. The data shown are mean ± SEM (n = 7–8). Scale bar, 50 μm. Statistical analysis was carried out using the Mann-Whitney U test ( ### P < 0.001 for sham vs isotype; ** P < 0.01 for isotype vs BB5.1). IR, ischemia-reperfusion; IRI, IR injury; RawIntDen, raw integrated density; SEM, standard error of the mean.
    Figure Legend Snippet: C5 blockade reduced renal IR-mediated complement activation and deposition. Twenty-four hours after reperfusion, kidney sections were analyzed for deposition of complement C3b/c (A) and C9 (C) using immunofluorescence staining/confocal microscopy, and fluorescence intensities were measured using Image J software (B and D). Nuclei were stained with DAPI. Tubular and glomerular epithelial and vascular endothelial C3b/c deposition (A) and tubular C9 deposition (C) were observed in isotype-treated mice. Treatment with BB5.1 reduced deposition of C3b/c (A and B) and C9 (C and D) compared with isotype control treatment. The data shown are mean ± SEM (n = 7–8). Scale bar, 50 μm. Statistical analysis was carried out using the Mann-Whitney U test ( ### P < 0.001 for sham vs isotype; ** P < 0.01 for isotype vs BB5.1). IR, ischemia-reperfusion; IRI, IR injury; RawIntDen, raw integrated density; SEM, standard error of the mean.

    Techniques Used: Activation Assay, Immunofluorescence, Staining, Confocal Microscopy, Fluorescence, Software, MANN-WHITNEY

    C5 blockade reduced the elevation of plasma C3b and C5a following renal IR. Twenty-four hours after reperfusion, plasma C3b (A) and C5a (B) were significantly higher in isotype-treated mice compared to sham-operated mice. Treatment with BB5.1 significantly attenuated this increase. The data shown are mean ± SEM (n = 7-8). Statistical analysis was carried out using the Mann-Whitney U test ( ## P < 0.01, ## P < 0.001 for sham vs isotype; ** P < 0.01 for isotype vs BB5.1). IR, ischemia-reperfusion; IRI, IR injury; SEM, standard error of the mean.
    Figure Legend Snippet: C5 blockade reduced the elevation of plasma C3b and C5a following renal IR. Twenty-four hours after reperfusion, plasma C3b (A) and C5a (B) were significantly higher in isotype-treated mice compared to sham-operated mice. Treatment with BB5.1 significantly attenuated this increase. The data shown are mean ± SEM (n = 7-8). Statistical analysis was carried out using the Mann-Whitney U test ( ## P < 0.01, ## P < 0.001 for sham vs isotype; ** P < 0.01 for isotype vs BB5.1). IR, ischemia-reperfusion; IRI, IR injury; SEM, standard error of the mean.

    Techniques Used: MANN-WHITNEY

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    Hycult Biotech c3b, mouse, elisa kit
    C3b, Mouse, Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech mouse c3b elisa kit
    Attenuation of IR-induced systemic complement activation products <t>C3b</t> and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by <t>ELISA.</t> Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).
    Mouse C3b Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse c3b elisa kit/product/Hycult Biotech
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    Hycult Biotech mouse c3b
    Attenuation of IR-induced systemic complement activation products <t>C3b</t> and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by <t>ELISA.</t> Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).
    Mouse C3b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Attenuation of IR-induced systemic complement activation products C3b and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by ELISA. Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).

    Journal: Scientific Reports

    Article Title: A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia–reperfusion injury

    doi: 10.1038/s41598-021-01423-y

    Figure Lengend Snippet: Attenuation of IR-induced systemic complement activation products C3b and C5a by CSL040 treatment. Plasma samples collected 24 h after reperfusion were analysed for ( A ) C3b, and ( B ) C5a by ELISA. Significance was tested using Mann–Whitney U test ( ### p < 0.001 for sham vs. IRI/vehicle), and One-way ANOVA Kruskal–Wallis and Dunn multiple comparisons test (**p < 0.01 for IRI/vehicle vs. all treatments). The data shown are mean ± SEM (n = 7–8 per group).

    Article Snippet: Plasma samples were analysed for C3b using the mouse C3b ELISA kit (HK216, Hycult Biotech) and for C5a using the Mouse Complement Activation Component C5a DuoSet ELISA kit (DY2150; R&D Systems, Minneapolis, MN) as per the manufacturer’s instructions.

    Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY