c1q elisa kit  (Hycult Biotech)

Journal: Scientific Reports

Article Title: Angiotensin II type 1a receptor deficiency alleviates muscle atrophy after denervation

doi: 10.1038/s41598-023-27737-7

Figure Lengend Snippet: AT1a receptor loss alleviated denervation-induced muscle atrophy independently of C1q/Wnt/β-catenin signaling in gastrocnemius muscle. ( a , b ) Gene expressions of C1qa ( a ) and Axin2 ( b ) were evaluated by RT-qPCR. Expression levels of these transcripts in each sample were normalized to 18S rRNA expression levels and were shown as the fold increase or decrease in mRNA expression in the Den group compared with the Cont group. ( c ) Serum C1q concentration was measured by ELISA. The graphs show the fold increase or decrease in serum concentration levels in the Den group compared with the Cont group. AT1a +/+ -Cont group, n = 6; AT1a +/+ -Den group at 7 days postdenervation, n = 7; AT1a +/+ -Den group at 21 days postdenervation, n = 7; AT1a −/− -Cont group, n = 5; AT1a −/− -Den group at 7 days postdenervation, n = 6; AT1a −/− -Den group at 21 days postdenervation, n = 6. Values are means ± SE.

Article Snippet: Serum complement C1q concentration was measured using a C1q, mouse, ELISA kit (Hycult Biotech, Uden, The Netherlands), which was the same as the kit used in the previous study showing the beneficial effects of Ang II receptor blockade against muscle injury .

Techniques: Quantitative RT-PCR, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay