hk  (Worthington Biochemical)


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  • 88

    Structured Review

    Worthington Biochemical hk
    Hk, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hk/product/Worthington Biochemical
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hk - by Bioz Stars, 2020-05
    88/100 stars

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    Isolation:

    Article Title: Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux
    Article Snippet: .. Use of the 2DOG ATP energy clamp to quantify ATP production in isolated mitochondria and simultaneous assessment of H2 O2 production Mitochondria (0.1 mg/ml) were added to individual wells of black polystyrene 96-well round bottom plates in a total volume of 60 μl and incubated at 37°C in respiratory buffer plus 5 units/ml HK (Worthington Biochemical) and 5 mM 2DOG in the presence of selected concentrations of ADP. .. After incubation for 20 min with gentle rocking, the contents of the microplate wells were removed to tubes on ice containing 1 μl of 120 μM oligomycin to inhibit ATP synthase.

    Incubation:

    Article Title: Mitochondrial Function in Diabetes: Novel Methodology and New Insight
    Article Snippet: .. Mitochondria (0.1 mg/mL) were added to individual wells of 96-well plates in a total volume of 60 µL and incubated at 37°C in respiratory buffer plus 5 units/mL HK (Worthington Biochemical) and 5 mmol/L 2DOG or, in some experiments, [6-13 C]2DOG (Cambridge Isotope Laboratories, Andover MA) in the presence of ADP or ATP ranging from 0 to 1,000 μmol/L, depending on the particular experiment. .. After incubation for the desired time, the contents of the microplate wells were removed to tubes on ice containing 1 μL of 120 µmol/L oligomycin to inhibit ATP synthase.

    Article Title: Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux
    Article Snippet: .. Use of the 2DOG ATP energy clamp to quantify ATP production in isolated mitochondria and simultaneous assessment of H2 O2 production Mitochondria (0.1 mg/ml) were added to individual wells of black polystyrene 96-well round bottom plates in a total volume of 60 μl and incubated at 37°C in respiratory buffer plus 5 units/ml HK (Worthington Biochemical) and 5 mM 2DOG in the presence of selected concentrations of ADP. .. After incubation for 20 min with gentle rocking, the contents of the microplate wells were removed to tubes on ice containing 1 μl of 120 μM oligomycin to inhibit ATP synthase.

    Article Title: Dietary fat, fatty acid saturation and mitochondrial bioenergetics
    Article Snippet: .. Mitochondria (0.5 mg/ml for liver or 0.1 mg/ml for heart) were added to individual wells of 96-well plates in a total volume of 60 μl and incubated at 37°C in respiratory buffer plus 5 units/ml HK (Worthington Biochemical) and 5 mM 2DOG in the presence of selected concentrations of ADP and fueled by the combined substrates, 5 mM succinate + 5 mM glutamate + 1 mM malate. .. After incubation for 20 min, the contents of the microplate wells were removed to tubes on ice containing 1μl of 120 μM oligomycin to inhibit ATP synthase.

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  • 91
    Worthington Biochemical choi hk
    Choi Hk, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Worthington Biochemical hk
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    https://www.bioz.com/result/hk/product/Worthington Biochemical
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    Worthington Biochemical hk activity
    Hk Activity, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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