2dog atp energy clamp (Worthington Biochemical)


88
Structured Review
Worthington Biochemical
2dog atp energy clamp
![The 2-deoxyglucose <t>(2DOG)</t> energy clamp. (A) Graphic depiction. Saturating amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle <t>ATP</t> back to ADP by rapidly and irreversibly converting 2DOG into 2-deoxyglucose phosphate (2DOGP). The ADP concentration is clamped at levels determined by the amount of ADP added. IMM = inner mitochondrial membrane. (B and C) Oxygraph tracings of inner membrane potential (inversely related to electrode potential shown on y-axis after calculation using the Nernst equation) vs. time obtained by incubating mouse hindlimb muscle mitochondria (mito), 0.05 mg/ml, fueled by 5 mM succinate (panel B) or 5 mM succinate plus 5 μM rotenone (panel C). ADP was added in incremental amounts to generate the final recycling nucleotide phosphate concentrations shown. After each addition, plateau values were reached, consistent with recycling at a steady [ADP].](https://storage.googleapis.com/bioz_article_images/PMC4859540/pone.0154982.g001.jpg)
2dog Atp Energy Clamp, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2dog atp energy clamp/product/Worthington Biochemical
Average 88 stars, based on 78 article reviews
Price from $9.99 to $1999.99
![The 2-deoxyglucose <t>(2DOG)</t> energy clamp. (A) Graphic depiction. Saturating amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle <t>ATP</t> back to ADP by rapidly and irreversibly converting 2DOG into 2-deoxyglucose phosphate (2DOGP). The ADP concentration is clamped at levels determined by the amount of ADP added. IMM = inner mitochondrial membrane. (B and C) Oxygraph tracings of inner membrane potential (inversely related to electrode potential shown on y-axis after calculation using the Nernst equation) vs. time obtained by incubating mouse hindlimb muscle mitochondria (mito), 0.05 mg/ml, fueled by 5 mM succinate (panel B) or 5 mM succinate plus 5 μM rotenone (panel C). ADP was added in incremental amounts to generate the final recycling nucleotide phosphate concentrations shown. After each addition, plateau values were reached, consistent with recycling at a steady [ADP].](https://storage.googleapis.com/bioz_article_images/PMC4859540/pone.0154982.g001.jpg)
2dog Atp Energy Clamp, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2dog atp energy clamp/product/Worthington Biochemical
Average 88 stars, based on 78 article reviews
Price from $9.99 to $1999.99
2dog atp energy clamp - by Bioz Stars,
2021-01
88/100 stars
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Images
1) Product Images from "Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux"
Article Title: Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux
Journal: PLoS ONE
doi: 10.1371/journal.pone.0154982
![The 2-deoxyglucose (2DOG) energy clamp. (A) Graphic depiction. Saturating amounts of ... The 2-deoxyglucose (2DOG) energy clamp. (A) Graphic depiction. Saturating amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle ATP back to ADP by rapidly and irreversibly converting 2DOG into 2-deoxyglucose phosphate (2DOGP). The ADP concentration is clamped at levels determined by the amount of ADP added. IMM = inner mitochondrial membrane. (B and C) Oxygraph tracings of inner membrane potential (inversely related to electrode potential shown on y-axis after calculation using the Nernst equation) vs. time obtained by incubating mouse hindlimb muscle mitochondria (mito), 0.05 mg/ml, fueled by 5 mM succinate (panel B) or 5 mM succinate plus 5 μM rotenone (panel C). ADP was added in incremental amounts to generate the final recycling nucleotide phosphate concentrations shown. After each addition, plateau values were reached, consistent with recycling at a steady [ADP].](https://storage.googleapis.com/bioz_article_images/PMC4859540/pone.0154982.g001.jpg)
Figure Legend Snippet: The 2-deoxyglucose (2DOG) energy clamp. (A) Graphic depiction. Saturating amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle ATP back to ADP by rapidly and irreversibly converting 2DOG into 2-deoxyglucose phosphate (2DOGP). The ADP concentration is clamped at levels determined by the amount of ADP added. IMM = inner mitochondrial membrane. (B and C) Oxygraph tracings of inner membrane potential (inversely related to electrode potential shown on y-axis after calculation using the Nernst equation) vs. time obtained by incubating mouse hindlimb muscle mitochondria (mito), 0.05 mg/ml, fueled by 5 mM succinate (panel B) or 5 mM succinate plus 5 μM rotenone (panel C). ADP was added in incremental amounts to generate the final recycling nucleotide phosphate concentrations shown. After each addition, plateau values were reached, consistent with recycling at a steady [ADP].
Techniques Used: Concentration Assay
2) Product Images from "Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux"
Article Title: Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux
Journal: PLoS ONE
doi: 10.1371/journal.pone.0154982
![... amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle ATP back to ADP by rapidly and irreversibly converting ... The 2-deoxyglucose (2DOG) energy clamp. (A) Graphic depiction. Saturating amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle ATP back to ADP by rapidly and irreversibly converting 2DOG into 2-deoxyglucose phosphate (2DOGP). The ADP concentration is clamped at levels determined by the amount of ADP added. IMM = inner mitochondrial membrane. (B and C) Oxygraph tracings of inner membrane potential (inversely related to electrode potential shown on y-axis after calculation using the Nernst equation) vs. time obtained by incubating mouse hindlimb muscle mitochondria (mito), 0.05 mg/ml, fueled by 5 mM succinate (panel B) or 5 mM succinate plus 5 μM rotenone (panel C). ADP was added in incremental amounts to generate the final recycling nucleotide phosphate concentrations shown. After each addition, plateau values were reached, consistent with recycling at a steady [ADP].](https://storage.googleapis.com/bioz_article_images/PMC4859540/pone.0154982.g001.jpg)
Figure Legend Snippet: The 2-deoxyglucose (2DOG) energy clamp. (A) Graphic depiction. Saturating amounts of 2-deoxyglucose (2DOG) and hexokinase (HK) recycle ATP back to ADP by rapidly and irreversibly converting 2DOG into 2-deoxyglucose phosphate (2DOGP). The ADP concentration is clamped at levels determined by the amount of ADP added. IMM = inner mitochondrial membrane. (B and C) Oxygraph tracings of inner membrane potential (inversely related to electrode potential shown on y-axis after calculation using the Nernst equation) vs. time obtained by incubating mouse hindlimb muscle mitochondria (mito), 0.05 mg/ml, fueled by 5 mM succinate (panel B) or 5 mM succinate plus 5 μM rotenone (panel C). ADP was added in incremental amounts to generate the final recycling nucleotide phosphate concentrations shown. After each addition, plateau values were reached, consistent with recycling at a steady [ADP].
Techniques Used: Concentration Assay
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