hk (Worthington Biochemical)

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Worthington Biochemical hk
Hk, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hk/product/Worthington Biochemical
Average 88 stars, based on 3 article reviews
Price from $9.99 to $1999.99

hk - by Bioz Stars, 2020-08

88/100 stars

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Isolation:

Article Title: Voltage-Dependent Regulation of Complex II Energized Mitochondrial Oxygen Flux
Article Snippet: .. Use of the 2DOG ATP energy clamp to quantify ATP production in isolated mitochondria and simultaneous assessment of H2 O2 production Mitochondria (0.1 mg/ml) were added to individual wells of black polystyrene 96-well round bottom plates in a total volume of 60 μl and incubated at 37°C in respiratory buffer plus 5 units/ml HK (Worthington Biochemical) and 5 mM 2DOG in the presence of selected concentrations of ADP. .. After incubation for 20 min with gentle rocking, the contents of the microplate wells were removed to tubes on ice containing 1 μl of 120 μM oligomycin to inhibit ATP synthase.

Incubation:

Article Title: Dietary fat, fatty acid saturation and mitochondrial bioenergetics
Article Snippet: .. Mitochondria (0.5 mg/ml for liver or 0.1 mg/ml for heart) were added to individual wells of 96-well plates in a total volume of 60 μl and incubated at 37°C in respiratory buffer plus 5 units/ml HK (Worthington Biochemical) and 5 mM 2DOG in the presence of selected concentrations of ADP and fueled by the combined substrates, 5 mM succinate + 5 mM glutamate + 1 mM malate. .. After incubation for 20 min, the contents of the microplate wells were removed to tubes on ice containing 1μl of 120 μM oligomycin to inhibit ATP synthase.

other:

Article Title: The role of the transcription factor Ets1 in lupus and other autoimmune diseases
Article Snippet: Okada Y, Wu D, Trynka G, Raj T, Terao C, Ikari K, Kochi Y, Ohmura K, Suzuki A, Yoshida S, Graham RR, Manoharan A, Ortmann W, Bhangale T, Denny JC, Carroll RJ, Eyler AE, Greenberg JD, Kremer JM, Pappas DA, Jiang L, Yin J, Ye L, Su DF, Yang J, Xie G, Keystone E, Westra HJ, Esko T, Metspalu A, Zhou X, Gupta N, Mirel D, Stahl EA, Diogo D, Cui J, Liao K, Guo MH, Myouzen K, Kawaguchi T, Coenen MJ, van Riel PL, van de Laar MA, Guchelaar HJ, Huizinga TW, Dieude P, Mariette X, Bridges SL, Jr, Zhernakova A, Toes RE, Tak PP, Miceli-Richard C, Bang SY, Lee HS, Martin J, Gonzalez-Gay MA, Rodriguez-Rodriguez L, Rantapaa-Dahlqvist S, Arlestig L, Choi HK, Kamatani Y, Galan P, Lathrop M, Eyre S, Bowes J, Barton A, de Vries N, Moreland LW, Criswell LA, Karlson EW, Taniguchi A, Yamada R, Kubo M, Liu JS, Bae SC, Worthington J, Padyukov L, Klareskog L, Gregersen PK, Raychaudhuri S, Stranger BE, De Jager PL, Franke L, Visscher PM, Brown MA, Yamanaka H, Mimori T, Takahashi A, Xu H, Behrens TW, Siminovitch KA, Momohara S, Matsuda F, Yamamoto K, Plenge RM.

Article Title: Integrated analysis of human genetic association study and mouse transcriptome suggests LBH and SHF genes as novel susceptible genes for amyloid-β accumulation in Alzheimer’s disease
Article Snippet: [ ] [ ] [ ] Okada Y, Wu D, Trynka G, Raj T, Terao C, Ikari K, Kochi Y, Ohmura K, Suzuki A, Yoshida S, Graham RR, Manoharan A, Ortmann W, Bhangale T, Denny JC, Carroll RJ, Eyler AE, Greenberg JD, Kremer JM, Pappas DA, Jiang L, Yin J, Ye L, Su DF, Yang J, Xie G, Keystone E, Westra HJ, Esko T, Metspalu A, Zhou X, Gupta N, Mirel D, Stahl EA, Diogo D, Cui J, Liao K, Guo MH, Myouzen K, Kawaguchi T, Coenen MJ, van Riel PL, van de Laar MA, Guchelaar HJ, Huizinga TW, Dieude P, Mariette X, Bridges SL, Jr, Zhernakova A, Toes RE, Tak PP, Miceli-Richard C, Bang SY, Lee HS, Martin J, Gonzalez-Gay MA, Rodriguez-Rodriguez L, Rantapaa-Dahlqvist S, Arlestig L, Choi HK, Kamatani Y, Galan P, Lathrop M, consortium R, consortium G, Eyre S, Bowes J, Barton A, de Vries N, Moreland LW, Criswell LA, Karlson EW, Taniguchi A, Yamada R, Kubo M, Liu JS, Bae SC, Worthington J, Padyukov L, Klareskog L, Gregersen PK, Raychaudhuri S, Stranger BE, De Jager PL, Franke L, Visscher PM, Brown MA, Yamanaka H, Mimori T, Takahashi A, Xu H, Behrens TW, Siminovitch KA, Momohara S, Matsuda F, Yamamoto K, Plenge RM.

Activity Assay:

Article Title: Poly(ADP-ribose) polymerase-dependent energy depletion occurs through inhibition of glycolysis
Article Snippet: .. HK activity was measured according to the Worthington protocol (Worthington Biochemical Corp.) with slight modifications. .. Antibodies against PAR polymer were generated using the Human Combinatorial Antibody Library (HuCAL) platform (Bio-Rad AbD Serotec, Germany).