human immune virus reverse transcriptase hivrt (Worthington Biochemical)

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Worthington Biochemical human immune virus reverse transcriptase hivrt
Human Immune Virus Reverse Transcriptase Hivrt, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human immune virus reverse transcriptase hivrt/product/Worthington Biochemical
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99

human immune virus reverse transcriptase hivrt - by Bioz Stars, 2021-01

94/100 stars

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Concentration Assay:

Article Title: RNase H sequence preferences influence antisense oligonucleotide efficiency
Article Snippet: .. Reactions with HIV-1 Reverse Transcriptase from Worthington Biochemical Corporations (cat. LS05003) were performed as human RNase H1, with the final concentration of the enzyme 0.1 U/μl. .. Control hydrolysis reactions labeled ‘EDTA’ were performed using identical conditions, but in the presence of 10 mM EDTA, whereas control reactions performed in ‘cold’ conditions were incubated for 30 min at 16°C.

Incubation:

Article Title: Transcriptome-wide Mapping of Internal N7-methylguanosine Methylome in Mammalian Messenger RNA
Article Snippet: .. The reactions were then prepared with RNA mixture, 2 μL 10 mM dNTP (Deoxynucleotide (dNTP) Solution Mix, NEB), 1X AMV Reverse Transcriptase Reaction Buffer (NEB), 1–2 μL Recombinant HIV reverse transcriptase (Worthington), and 1 μL RNaseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific) and then incubated at 37 °C for 1 hour. .. The cDNAs were purified with Oligo Clean and Concentrator (Zymo Research).

Recombinant:

Article Title: 3-O-Methylfunicone, a Selective Inhibitor of Mammalian Y-Family DNA Polymerases from an Australian Sea Salt Fungal Strain
Article Snippet: .. HIV-1 reverse transcriptase (recombinant) and the Klenow fragment of pol I from E. coli were purchased from Worthington Biochemical Corp. (Freehold, NJ, USA). .. T4 pol, Taq pol, T7 RNA polymerase and T4 polynucleotide kinase were purchased from Takara Bio (Tokyo, Japan).

Article Title: Sequence, Distance, and Accessibility are Determinants of 5? End-Directed Cleavages by Retroviral RNases H *
Article Snippet: .. Enzymes and reagents —Recombinant HIV-1 reverse transcriptase was obtained from Worthington Biochemicals. .. Recombinant wild-type M-MuLV reverse transcriptase, T7 DNA polymerase, and calf intestinal alkaline phosphatase were purchased from Amersham Pharmacia Biotech.

Activity Assay:

Article Title: Evaluation of the Broad-Range PCR-Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) System and Virus Microarrays for Virus Detection
Article Snippet: .. The number of particles in the original stock was determined as the average of the calculated particles at each dilution based upon a standard curve using HIV-1 RT enzyme (Worthington Biochemical Corporation Lakewood, NJ, USA, cat. No. LS05006; lot No. X1H2839), which was determined to have 262 pU of RT activity per particle [ ]. .. The STF-PERT one-step assay [ ] was modified into a two-step assay that no longer requires the use of AmpliWax® PCR Gem 50, a product that has been discontinued.