human immune virus reverse transcriptase hivrt (Worthington Biochemical)

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Name:

Reverse Transcriptase Recombinant HIV

Description:

Chromatographically purified dimeric form with M W of 66 kDa and 51 kDa A solution in 10 mM potassium phosphate pH 7 4 1 mM DTT and 20 glycerol

Catalog Number:

LS05000

Price:

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Source:

E. coli plasmid pRC-RT

Size:

Bulk

Cas Number:

9068.38.6

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Structured Review

Worthington Biochemical human immune virus reverse transcriptase hivrt
Chromatographically purified dimeric form with M W of 66 kDa and 51 kDa A solution in 10 mM potassium phosphate pH 7 4 1 mM DTT and 20 glycerol
https://www.bioz.com/result/human immune virus reverse transcriptase hivrt/product/Worthington Biochemical
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99

human immune virus reverse transcriptase hivrt - by Bioz Stars, 2021-04

92/100 stars

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Recombinant:

Article Title: Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized mice
Article Snippet: .. All samples were run simultaneously and concurrently with a recombinant HIV-1 reverse transcriptase standard curve (Worthington Biochemical Corporation, Lakewood, NJ). .. Statistics All descriptive statistics, one-way ANOVA to determine significance in CD4 surface down-modulation experiments to test Nef functionality, and Pearson correlation calculations to determine an association between p24+ cells and bioluminescent signal during longitudinal imaging of Hu-PBL mice were performed using GraphPad Prism software, version 7 (San Diego, CA).

Article Title: Sequence, Distance, and Accessibility are Determinants of 5? End-Directed Cleavages by Retroviral RNases H *
Article Snippet: .. Enzymes and reagents —Recombinant HIV-1 reverse transcriptase was obtained from Worthington Biochemicals. .. Recombinant wild-type M-MuLV reverse transcriptase, T7 DNA polymerase, and calf intestinal alkaline phosphatase were purchased from Amersham Pharmacia Biotech.

Article Title: Continuous Signal Enhancement for Sensitive Aptamer Affinity Probe Electrophoresis Assay Using Electrokinetic Concentration
Article Snippet: Human myeloma IgE was purchased from Athens Research and Technology, Inc. (Athens, GA). .. Recombinant HIV-1 reverse transcriptase (HIV-1 RT) was purchased from Worthington Biochemical Corporation (Lakewood, NJ). ..

Synthesized:

Article Title: Structural Insights into the HIV-1 Minus-strand Strong-stop DNA *
Article Snippet: .. Therefore, we synthesized the full-length ssDNA derived from the MAL isolate using HIV-1 reverse transcriptase. .. The 5′-end labeled ssDNA was synthesized and purified as described under “Experimental Procedures” section.

Derivative Assay:

Concentration Assay:

Article Title: RNase H sequence preferences influence antisense oligonucleotide efficiency
Article Snippet: Reactions with E. coli RNase H from Thermo Scientific (cat. EN0201) were performed at 37°C in a buffer composed of 50 mM Tris–HCl pH 8.3, 75 mM KCl, 3 mM MgCl2 , 10 mM dithiothreitol (DTT), 0.4 mg/ml bovine serum albumin and 0.1 mM EDTA using the same protocol as for the reactions with human-derived enzyme, but with a final enzyme concentration 0.5 mU/μl. .. Reactions with HIV-1 Reverse Transcriptase from Worthington Biochemical Corporations (cat. LS05003) were performed as human RNase H1, with the final concentration of the enzyme 0.1 U/μl. .. Control hydrolysis reactions labeled ‘EDTA’ were performed using identical conditions, but in the presence of 10 mM EDTA, whereas control reactions performed in ‘cold’ conditions were incubated for 30 min at 16°C.