dnase i  (Worthington Biochemical)


Bioz Verified Symbol Worthington Biochemical is a verified supplier
Bioz Manufacturer Symbol Worthington Biochemical manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    ls002004
    Price:
    33
    Size:
    5 mg
    Source:
    Bovine Pancreas
    Cas Number:
    9003.98.9
    Buy from Supplier


    Structured Review

    Worthington Biochemical dnase i
    Summary of <t>DNase</t> I protection results in the absence (A) or presence (B to E) of coinducers, including (B) RuBP, (C) NADPH, (D) ATP, and (E) FBP, each at 500 μM. Results are compared to a BSA control. Putative CbbR binding sites are indicated
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/dnase i/product/Worthington Biochemical
    Average 99 stars, based on 1007 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Further Unraveling the Regulatory Twist by Elucidating Metabolic Coinducer-Mediated CbbR-cbbI Promoter Interactions in Rhodopseudomonas palustris CGA010"

    Article Title: Further Unraveling the Regulatory Twist by Elucidating Metabolic Coinducer-Mediated CbbR-cbbI Promoter Interactions in Rhodopseudomonas palustris CGA010

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.06418-11

    Summary of DNase I protection results in the absence (A) or presence (B to E) of coinducers, including (B) RuBP, (C) NADPH, (D) ATP, and (E) FBP, each at 500 μM. Results are compared to a BSA control. Putative CbbR binding sites are indicated
    Figure Legend Snippet: Summary of DNase I protection results in the absence (A) or presence (B to E) of coinducers, including (B) RuBP, (C) NADPH, (D) ATP, and (E) FBP, each at 500 μM. Results are compared to a BSA control. Putative CbbR binding sites are indicated

    Techniques Used: Binding Assay

    DNase I footprinting of the CbbR binding sites on the cbbLS region in the presence of coinducer. The DNase I-digested reactions were prepared and analyzed by capillary electrophoresis with a 3730 DNA analyzer (Applied Biosystems). The differences in the
    Figure Legend Snippet: DNase I footprinting of the CbbR binding sites on the cbbLS region in the presence of coinducer. The DNase I-digested reactions were prepared and analyzed by capillary electrophoresis with a 3730 DNA analyzer (Applied Biosystems). The differences in the

    Techniques Used: Footprinting, Binding Assay, Electrophoresis

    2) Product Images from "Lysyl Oxidase Binds Transforming Growth Factor-? and Regulates Its Signaling via Amine Oxidase Activity *"

    Article Title: Lysyl Oxidase Binds Transforming Growth Factor-? and Regulates Its Signaling via Amine Oxidase Activity *

    Journal:

    doi: 10.1074/jbc.M803142200

    Effect of exogenous addition of LOX-V5 on TGF-β signaling in MC cells. Without rhTGF-β1, Smad3 phosphorylation was not induced in MC cells ( lane 1 ) and those treated with LOX-V5 ( lane 2 ) or BAPN ( lane 3 ). The phosphorylation was induced
    Figure Legend Snippet: Effect of exogenous addition of LOX-V5 on TGF-β signaling in MC cells. Without rhTGF-β1, Smad3 phosphorylation was not induced in MC cells ( lane 1 ) and those treated with LOX-V5 ( lane 2 ) or BAPN ( lane 3 ). The phosphorylation was induced

    Techniques Used:

    3) Product Images from "Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci"

    Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2017.01.017

    PARP-1 stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p
    Figure Legend Snippet: PARP-1 stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p

    Techniques Used: Binding Assay, In Vitro, Labeling, Incubation, Recombinant, Western Blot, Footprinting, Mutagenesis, Software

    PARP-1 is required for maintaining transcriptional program in embryonic stem cells A) Western blots showing the relative levels of three pluripotency factors (Sox2, Oct4, and Nanog) and PARP-1 in WT and Parp1 −/− mESCs. Actin is used as an internal loading control. B) Effect of Parp1 knockout on gene expression in mESCs as determined by RNA-seq. The heatmap shows the relative expression levels of genes whose expression significantly (FDR
    Figure Legend Snippet: PARP-1 is required for maintaining transcriptional program in embryonic stem cells A) Western blots showing the relative levels of three pluripotency factors (Sox2, Oct4, and Nanog) and PARP-1 in WT and Parp1 −/− mESCs. Actin is used as an internal loading control. B) Effect of Parp1 knockout on gene expression in mESCs as determined by RNA-seq. The heatmap shows the relative expression levels of genes whose expression significantly (FDR

    Techniques Used: Western Blot, Knock-Out, Expressing, RNA Sequencing Assay

    PARP-1 is required for the binding of Sox2 to a subset of its genomic sites in mESCs A) Genome browser tracks of Sox2 ChIP-seq data around the Nanog and Pou5f1 (encoding Oct4) genes in WT and Parp1 −/− mESCs. B) Left and middle , Heatmaps of Sox2 ChIP-seq signals in WT and Parp1 −/− mESCs centered on the Sox2 binding sites (± 2 kb) and ordered top to bottom by signal intensity. Right , PARP-1 ChIP-seq signals in WT mESCs associated with the corresponding Sox2 binding sites. PARP-1-dependent Sox2 binding sites are defined as those sites whose ChIP-seq signals are significantly decreased upon PARP-1 knockout (p-value
    Figure Legend Snippet: PARP-1 is required for the binding of Sox2 to a subset of its genomic sites in mESCs A) Genome browser tracks of Sox2 ChIP-seq data around the Nanog and Pou5f1 (encoding Oct4) genes in WT and Parp1 −/− mESCs. B) Left and middle , Heatmaps of Sox2 ChIP-seq signals in WT and Parp1 −/− mESCs centered on the Sox2 binding sites (± 2 kb) and ordered top to bottom by signal intensity. Right , PARP-1 ChIP-seq signals in WT mESCs associated with the corresponding Sox2 binding sites. PARP-1-dependent Sox2 binding sites are defined as those sites whose ChIP-seq signals are significantly decreased upon PARP-1 knockout (p-value

    Techniques Used: Binding Assay, Chromatin Immunoprecipitation, Knock-Out, Significance Assay

    PARP-1 colocalizes with Sox2 genome-wide A) Top, Summary of the known roles of PARP-1 protein and PARylation activity in embryonic stem cells. Bottom, Summary of known changes in Sox2 protein and PARylation levels, total cellular PARylation levels during embryonic stem cell differentiation. B) The level of PARP-1-mediated PARylation is very low in undifferentiated mESCs and increases upon differentiation. Western blots of poly(ADP-ribose) (PAR) and PARP-1 showing their relative levels in mESCs during a 9 day time course of differentiation upon LIF removal. C) Distribution of significant peaks of PARP-1 binding from ChIP-seq across genomic features in mESCs. D) Distribution of significant peaks of PARP-1 binding relative to the TSSs of all RefSeq genes. E) PARP-1 colocalizes with Sox2 genome-wide. Correlation matrix of genome-wide enrichment for chromatin- and transcription-related factors from ChIP-seq data in mESCs, organized and ordered using hierarchical clustering. F) Left , Heatmap of high confidence Sox2 peaks in mESCs from ChIP-seq data (n = 16,042; p-value
    Figure Legend Snippet: PARP-1 colocalizes with Sox2 genome-wide A) Top, Summary of the known roles of PARP-1 protein and PARylation activity in embryonic stem cells. Bottom, Summary of known changes in Sox2 protein and PARylation levels, total cellular PARylation levels during embryonic stem cell differentiation. B) The level of PARP-1-mediated PARylation is very low in undifferentiated mESCs and increases upon differentiation. Western blots of poly(ADP-ribose) (PAR) and PARP-1 showing their relative levels in mESCs during a 9 day time course of differentiation upon LIF removal. C) Distribution of significant peaks of PARP-1 binding from ChIP-seq across genomic features in mESCs. D) Distribution of significant peaks of PARP-1 binding relative to the TSSs of all RefSeq genes. E) PARP-1 colocalizes with Sox2 genome-wide. Correlation matrix of genome-wide enrichment for chromatin- and transcription-related factors from ChIP-seq data in mESCs, organized and ordered using hierarchical clustering. F) Left , Heatmap of high confidence Sox2 peaks in mESCs from ChIP-seq data (n = 16,042; p-value

    Techniques Used: Genome Wide, Activity Assay, Cell Differentiation, Western Blot, Binding Assay, Chromatin Immunoprecipitation

    4) Product Images from "Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity"

    Article Title: Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

    Journal: Immunity

    doi: 10.1016/j.immuni.2013.10.006

    PLA2 induces a Th2 response through cleavage of membrane phospholipids to produce lysophospholipids
    Figure Legend Snippet: PLA2 induces a Th2 response through cleavage of membrane phospholipids to produce lysophospholipids

    Techniques Used:

    Related Articles

    In Vitro:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Blocking Assay:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Produced:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Concentration Assay:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Incubation:

    Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
    Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of DNase I (DPRF; Worthington Biochemical Corporation, Lakewood, NJ) for 10 min at room temperature with gentle agitation. ..

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    other:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: The region is dA+dT rich, and protection seen from positions +126 to +129 is subtle because DNase I does not cleave DNA efficiently in this region.

    Article Title: Identification of Viral Peptide Fragments for Vaccine Development
    Article Snippet: In the random digestion step, the use of DNase I in the presence of MnCl2 is critical as this protocol will generate DNA fragments of relatively uniform sizes, which facilitates the reassembly step ( 17 , also see Note ).

    Activity Assay:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Expressing:

    Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
    Article Snippet: .. Analysis of nuclease sensitivity ( ) of the genomic histone HIST2H4 locus to DNase I reveals changes in chromatin structure that accompany increased histone gene expression in hES cells. ..

    Recombinant:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Worthington Biochemical his tagged human parp 1
    <t>PARP-1</t> stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p
    His Tagged Human Parp 1, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his tagged human parp 1/product/Worthington Biochemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    his tagged human parp 1 - by Bioz Stars, 2020-10
    91/100 stars
      Buy from Supplier

    91
    Worthington Biochemical shprh myc his
    <t>PARP-1</t> stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p
    Shprh Myc His, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shprh myc his/product/Worthington Biochemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shprh myc his - by Bioz Stars, 2020-10
    91/100 stars
      Buy from Supplier

    84
    Worthington Biochemical human intestinal enteroids hies
    <t>PARP-1</t> stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p
    Human Intestinal Enteroids Hies, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human intestinal enteroids hies/product/Worthington Biochemical
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human intestinal enteroids hies - by Bioz Stars, 2020-10
    84/100 stars
      Buy from Supplier

    Image Search Results


    PARP-1 stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p

    Journal: Molecular cell

    Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci

    doi: 10.1016/j.molcel.2017.01.017

    Figure Lengend Snippet: PARP-1 stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p

    Article Snippet: For these assays (see ), mononucleosomes corresponding to 375 ng of DNA were incubated with 300 ng of Sox2 protein with or without 600 ng of His-tagged human PARP-1 in 50 μL of binding buffer (25 mM HEPES pH 7.5, 100 mM KCl, 20% glycerol, 0.1% NP-40, 10 μM ZnSO4 , 1 mM DTT, 1x complete protease inhibitor cocktail) for 1 hour at 30°C, followed by the addition of 5 U of DNaseI (Worthington; DPFF grade, diluted in 50 μL of digestion buffer containing 10 mM MgCl2 and 5 mM CaCl2 ) and incubated at 25°C for 5 min.

    Techniques: Binding Assay, In Vitro, Labeling, Incubation, Recombinant, Western Blot, Footprinting, Mutagenesis, Software

    PARP-1 is required for maintaining transcriptional program in embryonic stem cells A) Western blots showing the relative levels of three pluripotency factors (Sox2, Oct4, and Nanog) and PARP-1 in WT and Parp1 −/− mESCs. Actin is used as an internal loading control. B) Effect of Parp1 knockout on gene expression in mESCs as determined by RNA-seq. The heatmap shows the relative expression levels of genes whose expression significantly (FDR

    Journal: Molecular cell

    Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci

    doi: 10.1016/j.molcel.2017.01.017

    Figure Lengend Snippet: PARP-1 is required for maintaining transcriptional program in embryonic stem cells A) Western blots showing the relative levels of three pluripotency factors (Sox2, Oct4, and Nanog) and PARP-1 in WT and Parp1 −/− mESCs. Actin is used as an internal loading control. B) Effect of Parp1 knockout on gene expression in mESCs as determined by RNA-seq. The heatmap shows the relative expression levels of genes whose expression significantly (FDR

    Article Snippet: For these assays (see ), mononucleosomes corresponding to 375 ng of DNA were incubated with 300 ng of Sox2 protein with or without 600 ng of His-tagged human PARP-1 in 50 μL of binding buffer (25 mM HEPES pH 7.5, 100 mM KCl, 20% glycerol, 0.1% NP-40, 10 μM ZnSO4 , 1 mM DTT, 1x complete protease inhibitor cocktail) for 1 hour at 30°C, followed by the addition of 5 U of DNaseI (Worthington; DPFF grade, diluted in 50 μL of digestion buffer containing 10 mM MgCl2 and 5 mM CaCl2 ) and incubated at 25°C for 5 min.

    Techniques: Western Blot, Knock-Out, Expressing, RNA Sequencing Assay

    PARP-1 is required for the binding of Sox2 to a subset of its genomic sites in mESCs A) Genome browser tracks of Sox2 ChIP-seq data around the Nanog and Pou5f1 (encoding Oct4) genes in WT and Parp1 −/− mESCs. B) Left and middle , Heatmaps of Sox2 ChIP-seq signals in WT and Parp1 −/− mESCs centered on the Sox2 binding sites (± 2 kb) and ordered top to bottom by signal intensity. Right , PARP-1 ChIP-seq signals in WT mESCs associated with the corresponding Sox2 binding sites. PARP-1-dependent Sox2 binding sites are defined as those sites whose ChIP-seq signals are significantly decreased upon PARP-1 knockout (p-value

    Journal: Molecular cell

    Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci

    doi: 10.1016/j.molcel.2017.01.017

    Figure Lengend Snippet: PARP-1 is required for the binding of Sox2 to a subset of its genomic sites in mESCs A) Genome browser tracks of Sox2 ChIP-seq data around the Nanog and Pou5f1 (encoding Oct4) genes in WT and Parp1 −/− mESCs. B) Left and middle , Heatmaps of Sox2 ChIP-seq signals in WT and Parp1 −/− mESCs centered on the Sox2 binding sites (± 2 kb) and ordered top to bottom by signal intensity. Right , PARP-1 ChIP-seq signals in WT mESCs associated with the corresponding Sox2 binding sites. PARP-1-dependent Sox2 binding sites are defined as those sites whose ChIP-seq signals are significantly decreased upon PARP-1 knockout (p-value

    Article Snippet: For these assays (see ), mononucleosomes corresponding to 375 ng of DNA were incubated with 300 ng of Sox2 protein with or without 600 ng of His-tagged human PARP-1 in 50 μL of binding buffer (25 mM HEPES pH 7.5, 100 mM KCl, 20% glycerol, 0.1% NP-40, 10 μM ZnSO4 , 1 mM DTT, 1x complete protease inhibitor cocktail) for 1 hour at 30°C, followed by the addition of 5 U of DNaseI (Worthington; DPFF grade, diluted in 50 μL of digestion buffer containing 10 mM MgCl2 and 5 mM CaCl2 ) and incubated at 25°C for 5 min.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Knock-Out, Significance Assay

    PARP-1 colocalizes with Sox2 genome-wide A) Top, Summary of the known roles of PARP-1 protein and PARylation activity in embryonic stem cells. Bottom, Summary of known changes in Sox2 protein and PARylation levels, total cellular PARylation levels during embryonic stem cell differentiation. B) The level of PARP-1-mediated PARylation is very low in undifferentiated mESCs and increases upon differentiation. Western blots of poly(ADP-ribose) (PAR) and PARP-1 showing their relative levels in mESCs during a 9 day time course of differentiation upon LIF removal. C) Distribution of significant peaks of PARP-1 binding from ChIP-seq across genomic features in mESCs. D) Distribution of significant peaks of PARP-1 binding relative to the TSSs of all RefSeq genes. E) PARP-1 colocalizes with Sox2 genome-wide. Correlation matrix of genome-wide enrichment for chromatin- and transcription-related factors from ChIP-seq data in mESCs, organized and ordered using hierarchical clustering. F) Left , Heatmap of high confidence Sox2 peaks in mESCs from ChIP-seq data (n = 16,042; p-value

    Journal: Molecular cell

    Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci

    doi: 10.1016/j.molcel.2017.01.017

    Figure Lengend Snippet: PARP-1 colocalizes with Sox2 genome-wide A) Top, Summary of the known roles of PARP-1 protein and PARylation activity in embryonic stem cells. Bottom, Summary of known changes in Sox2 protein and PARylation levels, total cellular PARylation levels during embryonic stem cell differentiation. B) The level of PARP-1-mediated PARylation is very low in undifferentiated mESCs and increases upon differentiation. Western blots of poly(ADP-ribose) (PAR) and PARP-1 showing their relative levels in mESCs during a 9 day time course of differentiation upon LIF removal. C) Distribution of significant peaks of PARP-1 binding from ChIP-seq across genomic features in mESCs. D) Distribution of significant peaks of PARP-1 binding relative to the TSSs of all RefSeq genes. E) PARP-1 colocalizes with Sox2 genome-wide. Correlation matrix of genome-wide enrichment for chromatin- and transcription-related factors from ChIP-seq data in mESCs, organized and ordered using hierarchical clustering. F) Left , Heatmap of high confidence Sox2 peaks in mESCs from ChIP-seq data (n = 16,042; p-value

    Article Snippet: For these assays (see ), mononucleosomes corresponding to 375 ng of DNA were incubated with 300 ng of Sox2 protein with or without 600 ng of His-tagged human PARP-1 in 50 μL of binding buffer (25 mM HEPES pH 7.5, 100 mM KCl, 20% glycerol, 0.1% NP-40, 10 μM ZnSO4 , 1 mM DTT, 1x complete protease inhibitor cocktail) for 1 hour at 30°C, followed by the addition of 5 U of DNaseI (Worthington; DPFF grade, diluted in 50 μL of digestion buffer containing 10 mM MgCl2 and 5 mM CaCl2 ) and incubated at 25°C for 5 min.

    Techniques: Genome Wide, Activity Assay, Cell Differentiation, Western Blot, Binding Assay, Chromatin Immunoprecipitation