dnase i (Worthington Biochemical)


Name:
Deoxyribonuclease I
Description:
Chromatographically purified A lyophilized powder with glycine as a stabilizer
Catalog Number:
ls002004
Price:
33
Size:
5 mg
Source:
Bovine Pancreas
Cas Number:
9003.98.9
|
Buy from Supplier |
Structured Review

Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnase i/product/Worthington Biochemical
Average 99 stars, based on 1006 article reviews
Price from $9.99 to $1999.99
Related Products / Commonly Used Together
Images
1) Product Images from "Further Unraveling the Regulatory Twist by Elucidating Metabolic Coinducer-Mediated CbbR-cbbI Promoter Interactions in Rhodopseudomonas palustris CGA010"
Article Title: Further Unraveling the Regulatory Twist by Elucidating Metabolic Coinducer-Mediated CbbR-cbbI Promoter Interactions in Rhodopseudomonas palustris CGA010
Journal: Journal of Bacteriology
doi: 10.1128/JB.06418-11

Figure Legend Snippet: Summary of DNase I protection results in the absence (A) or presence (B to E) of coinducers, including (B) RuBP, (C) NADPH, (D) ATP, and (E) FBP, each at 500 μM. Results are compared to a BSA control. Putative CbbR binding sites are indicated
Techniques Used: Binding Assay

Figure Legend Snippet: DNase I footprinting of the CbbR binding sites on the cbbLS region in the presence of coinducer. The DNase I-digested reactions were prepared and analyzed by capillary electrophoresis with a 3730 DNA analyzer (Applied Biosystems). The differences in the
Techniques Used: Footprinting, Binding Assay, Electrophoresis
2) Product Images from "Lysyl Oxidase Binds Transforming Growth Factor-? and Regulates Its Signaling via Amine Oxidase Activity *"
Article Title: Lysyl Oxidase Binds Transforming Growth Factor-? and Regulates Its Signaling via Amine Oxidase Activity *
Journal:
doi: 10.1074/jbc.M803142200

Figure Legend Snippet: Effect of exogenous addition of LOX-V5 on TGF-β signaling in MC cells. Without rhTGF-β1, Smad3 phosphorylation was not induced in MC cells ( lane 1 ) and those treated with LOX-V5 ( lane 2 ) or BAPN ( lane 3 ). The phosphorylation was induced
Techniques Used:
3) Product Images from "Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci"
Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci
Journal: Molecular cell
doi: 10.1016/j.molcel.2017.01.017

Figure Legend Snippet: PARP-1 stabilizes Sox2 binding to nucleosome in vitro A) In vitro nucleosome binding assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Ten nM of biotin-labeled 601 NPE DNA (207 bp) with or without a Sox motif (Sox+ or Sox−, respectively), or the same DNA assembled into a mononucleosome (Nuc), was immobilized on streptavidin beads and incubated ± recombinant Sox2 (10 nM) and PARP-1 (10 nM), as indicated. After washing, the bound proteins were analyzed by Western blotting. B) In vitro DNase I footprinting assays show PARP-1-dependent binding of Sox2 to nucleosomes, but not naked DNA. Left, Footprinting assay with naked 3x 601 NPE DNA containing a Sox motif. Right, Footprinting assay with a trinucleosome containing a Sox motif located at the dyad axis of the middle nucleosome. Addition of 10 nM WT PARP-1, but not a DNA binding domain mutant PARP-1 (DBDmut), enhances Sox2 (25 nM) binding, as indicated. PARP-1 binding to the nucleosome is also evident. C) Quantification of the DNase I footprinting assays shown in panel C. The bands marked with a red asterisk in panel B were quantified using a phosphorimager and ImageJ software. Each bar represents the mean plus the SEM, n = 3. Asterisks indicate significant differences versus the control (−Sox2) (Student’s t test, ** p
Techniques Used: Binding Assay, In Vitro, Labeling, Incubation, Recombinant, Western Blot, Footprinting, Mutagenesis, Software

Figure Legend Snippet: PARP-1 is required for maintaining transcriptional program in embryonic stem cells A) Western blots showing the relative levels of three pluripotency factors (Sox2, Oct4, and Nanog) and PARP-1 in WT and Parp1 −/− mESCs. Actin is used as an internal loading control. B) Effect of Parp1 knockout on gene expression in mESCs as determined by RNA-seq. The heatmap shows the relative expression levels of genes whose expression significantly (FDR
Techniques Used: Western Blot, Knock-Out, Expressing, RNA Sequencing Assay

Figure Legend Snippet: PARP-1 is required for the binding of Sox2 to a subset of its genomic sites in mESCs A) Genome browser tracks of Sox2 ChIP-seq data around the Nanog and Pou5f1 (encoding Oct4) genes in WT and Parp1 −/− mESCs. B) Left and middle , Heatmaps of Sox2 ChIP-seq signals in WT and Parp1 −/− mESCs centered on the Sox2 binding sites (± 2 kb) and ordered top to bottom by signal intensity. Right , PARP-1 ChIP-seq signals in WT mESCs associated with the corresponding Sox2 binding sites. PARP-1-dependent Sox2 binding sites are defined as those sites whose ChIP-seq signals are significantly decreased upon PARP-1 knockout (p-value
Techniques Used: Binding Assay, Chromatin Immunoprecipitation, Knock-Out, Significance Assay

Figure Legend Snippet: PARP-1 colocalizes with Sox2 genome-wide A) Top, Summary of the known roles of PARP-1 protein and PARylation activity in embryonic stem cells. Bottom, Summary of known changes in Sox2 protein and PARylation levels, total cellular PARylation levels during embryonic stem cell differentiation. B) The level of PARP-1-mediated PARylation is very low in undifferentiated mESCs and increases upon differentiation. Western blots of poly(ADP-ribose) (PAR) and PARP-1 showing their relative levels in mESCs during a 9 day time course of differentiation upon LIF removal. C) Distribution of significant peaks of PARP-1 binding from ChIP-seq across genomic features in mESCs. D) Distribution of significant peaks of PARP-1 binding relative to the TSSs of all RefSeq genes. E) PARP-1 colocalizes with Sox2 genome-wide. Correlation matrix of genome-wide enrichment for chromatin- and transcription-related factors from ChIP-seq data in mESCs, organized and ordered using hierarchical clustering. F) Left , Heatmap of high confidence Sox2 peaks in mESCs from ChIP-seq data (n = 16,042; p-value
Techniques Used: Genome Wide, Activity Assay, Cell Differentiation, Western Blot, Binding Assay, Chromatin Immunoprecipitation
4) Product Images from "Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity"
Article Title: Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity
Journal: Immunity
doi: 10.1016/j.immuni.2013.10.006

Figure Legend Snippet: PLA2 induces a Th2 response through cleavage of membrane phospholipids to produce lysophospholipids
Techniques Used:
Related Articles
In Vitro:Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis Article Snippet: .. Treatment with Positive Control:Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells Article Snippet: .. Concentration ranges for Immunoprecipitation:Article Title: Association of Herpes Simplex Virus Type 1 ICP8 and ICP27 Proteins with Cellular RNA Polymerase II Holoenzyme Article Snippet: .. To investigate this hypothesis, we treated lysates of wt HSV-1 KOS strain-infected HEp-2 cells with a cocktail of RNase A and RNase T1 or with Footprinting:Article Title: Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock Article Snippet: .. To map the C.Kpn2I binding site we used Blocking Assay:Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis Article Snippet: .. Treatment with Concentration Assay:Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells Article Snippet: .. Concentration ranges for Incubation:Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of Binding Assay:Article Title: Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock Article Snippet: .. To map the C.Kpn2I binding site we used |