gst tagged hdac proteins  (Worthington Biochemical)


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    Name:
    Nuclease Micrococcal
    Description:
    Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically SDS PAGE A lyophilized powder
    Catalog Number:
    ls004796
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    Source:
    Staphylococcus aureus (Strain Foggi)
    Cas Number:
    9013.53.0
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    Structured Review

    Worthington Biochemical gst tagged hdac proteins
    AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various <t>HDAC</t> proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to <t>GST.</t> HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .
    Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically SDS PAGE A lyophilized powder
    https://www.bioz.com/result/gst tagged hdac proteins/product/Worthington Biochemical
    Average 90 stars, based on 160 article reviews
    Price from $9.99 to $1999.99
    gst tagged hdac proteins - by Bioz Stars, 2020-10
    90/100 stars

    Images

    1) Product Images from "Telomere-binding protein regulates the chromosome ends through the interaction with histone deacetylases in Arabidopsis thaliana"

    Article Title: Telomere-binding protein regulates the chromosome ends through the interaction with histone deacetylases in Arabidopsis thaliana

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw067

    AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various HDAC proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to GST. HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .
    Figure Legend Snippet: AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various HDAC proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to GST. HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .

    Techniques Used: Histone Deacetylase Assay, Western Blot, Polyacrylamide Gel Electrophoresis, Purification, Pull Down Assay, Binding Assay, In Vivo, Chromatin Immunoprecipitation

    2) Product Images from "The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins"

    Article Title: The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2020.00331

    Visualization of nuclei at different steps of the protocol using a phase-contrast microscope. Isolated nuclei at Step 6 (A) MNase-digested nuclei at Step 9 (B) TE-resuspended nuclei at Step 11 (C) .
    Figure Legend Snippet: Visualization of nuclei at different steps of the protocol using a phase-contrast microscope. Isolated nuclei at Step 6 (A) MNase-digested nuclei at Step 9 (B) TE-resuspended nuclei at Step 11 (C) .

    Techniques Used: Microscopy, Isolation

    Related Articles

    DNA Extraction:

    Article Title: Natural Single-Nucleosome Epi-Polymorphisms in Yeast
    Article Snippet: .. We followed the protocol of Liu et al. for both nucleosomal DNA isolation and ChIP, except that incubation time with micrococcal nuclease (Worthington Biochemical) prior to immunopurification was increased to 30 min at 37°C to obtain mononucleosomes. .. ChIP was performed using 3 µl of anti-H3K14Ac polyclonal antibody (Upstate, 07–353).

    Amplification:

    Article Title: Genome-Wide Analysis of Nucleosome Positions, Occupancy, and Accessibility in Yeast: Nucleosome Mapping, High-Resolution Histone ChIP, and NCAM
    Article Snippet: .. Overnight saturated culture of S. cerevisiae strain 37% Formaldehyde 2.5 M Glycine Spheroblast solution (see recipe) β-mercaptoethanol (14.3 M) 100T zymolyase (AMSBIO) Micrococcal Nuclease (Worthington): Stored −80°C, 20U/μL in 10 mM Tris pH 7.4 Exonuclease III (NEB) MNase Digestion Buffer (See Recipe) MNase Stop Buffer (See Recipe) Proteinase K (20 mg/ml) 37°C incubator/water bath 65°C incubator Phenol:Chloroform:Isoamyl Alcohol (25:24:1) Ethanol (100%) 3M Sodium Acetate pH 5.2 Glycogen (20 mg/ml) NEB Buffer 2 RNase A (DNase-Free, 10 mg/ml) PCR Clean-up Kit (Qiagen) Alkaline Phosphatase (NEB) NEB Buffer 3 Low-melt Agarose (GeneMate) Gel Extraction Kit (Qiagen) TruSeq Sample Prep Kit (Illumina) [ or other desired library preparation kit ] MinElute PCR Purification Kit (Qiagen) Thermal Cycler for PCR Amplification ..

    Sample Prep:

    Article Title: Genome-Wide Analysis of Nucleosome Positions, Occupancy, and Accessibility in Yeast: Nucleosome Mapping, High-Resolution Histone ChIP, and NCAM
    Article Snippet: .. Overnight saturated culture of S. cerevisiae strain 37% Formaldehyde 2.5 M Glycine Spheroblast solution (see recipe) β-mercaptoethanol (14.3 M) 100T zymolyase (AMSBIO) Micrococcal Nuclease (Worthington): Stored −80°C, 20U/μL in 10 mM Tris pH 7.4 Exonuclease III (NEB) MNase Digestion Buffer (See Recipe) MNase Stop Buffer (See Recipe) Proteinase K (20 mg/ml) 37°C incubator/water bath 65°C incubator Phenol:Chloroform:Isoamyl Alcohol (25:24:1) Ethanol (100%) 3M Sodium Acetate pH 5.2 Glycogen (20 mg/ml) NEB Buffer 2 RNase A (DNase-Free, 10 mg/ml) PCR Clean-up Kit (Qiagen) Alkaline Phosphatase (NEB) NEB Buffer 3 Low-melt Agarose (GeneMate) Gel Extraction Kit (Qiagen) TruSeq Sample Prep Kit (Illumina) [ or other desired library preparation kit ] MinElute PCR Purification Kit (Qiagen) Thermal Cycler for PCR Amplification ..

    Isolation:

    Article Title: Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching
    Article Snippet: .. Nuclei were isolated, digested with micrococcal nuclease or DNaseI (Worthington), and DNA was purified as described ( ; ) with modifications detailed in . .. Naked DNA controls were obtained by digesting a PCR product.

    Purification:

    Article Title: Genome-Wide Analysis of Nucleosome Positions, Occupancy, and Accessibility in Yeast: Nucleosome Mapping, High-Resolution Histone ChIP, and NCAM
    Article Snippet: .. Overnight saturated culture of S. cerevisiae strain 37% Formaldehyde 2.5 M Glycine Spheroblast solution (see recipe) β-mercaptoethanol (14.3 M) 100T zymolyase (AMSBIO) Micrococcal Nuclease (Worthington): Stored −80°C, 20U/μL in 10 mM Tris pH 7.4 Exonuclease III (NEB) MNase Digestion Buffer (See Recipe) MNase Stop Buffer (See Recipe) Proteinase K (20 mg/ml) 37°C incubator/water bath 65°C incubator Phenol:Chloroform:Isoamyl Alcohol (25:24:1) Ethanol (100%) 3M Sodium Acetate pH 5.2 Glycogen (20 mg/ml) NEB Buffer 2 RNase A (DNase-Free, 10 mg/ml) PCR Clean-up Kit (Qiagen) Alkaline Phosphatase (NEB) NEB Buffer 3 Low-melt Agarose (GeneMate) Gel Extraction Kit (Qiagen) TruSeq Sample Prep Kit (Illumina) [ or other desired library preparation kit ] MinElute PCR Purification Kit (Qiagen) Thermal Cycler for PCR Amplification ..

    Article Title: Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching
    Article Snippet: .. Nuclei were isolated, digested with micrococcal nuclease or DNaseI (Worthington), and DNA was purified as described ( ; ) with modifications detailed in . .. Naked DNA controls were obtained by digesting a PCR product.

    Concentration Assay:

    Article Title: Histone hypoacetylation-activated genes are repressed by acetyl-CoA- and chromatin-mediated mechanism
    Article Snippet: .. Micrococcal nuclease (MNase; Worthington) was added immediately to a final concentration of 0, 1, 2.5, 10, 20, 50 U/ml. .. The samples were incubated for 5 min at 28°C with occasional gentle tapping of the microfuge tube.

    Incubation:

    Article Title: Ribosomal Pausing at a Frameshifter RNA Pseudoknot Is Sensitive to Reading Phase but Shows Little Correlation with Frameshift Efficiency
    Article Snippet: .. Unprotected mRNA was degraded by incubation with micrococcal nuclease (1 or 2 U/μl; Worthington) and RNase V1 (0.02 U/μl; Pharmacia) at 26°C for 30 min in the presence of 3.5 mM Mg(OAc)2 and 3 mM CaCl2 in a final reaction volume of 40 μl. .. Following addition of 60 μl of buffer T (20 mM HEPES, 150 mM KOAc, 10 mM Mg(OAc)2 , 5 mM EGTA, 2 mM dithiothreitol), the reaction mixture was overlayered onto a 60-μl cushion of 0.25 M sucrose in buffer T and subsequently centrifuged at 30 lb/in2 for 30 min in an A-110 rotor in a Beckman airfuge.

    Article Title: Natural Single-Nucleosome Epi-Polymorphisms in Yeast
    Article Snippet: .. We followed the protocol of Liu et al. for both nucleosomal DNA isolation and ChIP, except that incubation time with micrococcal nuclease (Worthington Biochemical) prior to immunopurification was increased to 30 min at 37°C to obtain mononucleosomes. .. ChIP was performed using 3 µl of anti-H3K14Ac polyclonal antibody (Upstate, 07–353).

    Polymerase Chain Reaction:

    Article Title: Genome-Wide Analysis of Nucleosome Positions, Occupancy, and Accessibility in Yeast: Nucleosome Mapping, High-Resolution Histone ChIP, and NCAM
    Article Snippet: .. Overnight saturated culture of S. cerevisiae strain 37% Formaldehyde 2.5 M Glycine Spheroblast solution (see recipe) β-mercaptoethanol (14.3 M) 100T zymolyase (AMSBIO) Micrococcal Nuclease (Worthington): Stored −80°C, 20U/μL in 10 mM Tris pH 7.4 Exonuclease III (NEB) MNase Digestion Buffer (See Recipe) MNase Stop Buffer (See Recipe) Proteinase K (20 mg/ml) 37°C incubator/water bath 65°C incubator Phenol:Chloroform:Isoamyl Alcohol (25:24:1) Ethanol (100%) 3M Sodium Acetate pH 5.2 Glycogen (20 mg/ml) NEB Buffer 2 RNase A (DNase-Free, 10 mg/ml) PCR Clean-up Kit (Qiagen) Alkaline Phosphatase (NEB) NEB Buffer 3 Low-melt Agarose (GeneMate) Gel Extraction Kit (Qiagen) TruSeq Sample Prep Kit (Illumina) [ or other desired library preparation kit ] MinElute PCR Purification Kit (Qiagen) Thermal Cycler for PCR Amplification ..

    Gel Extraction:

    Article Title: Genome-Wide Analysis of Nucleosome Positions, Occupancy, and Accessibility in Yeast: Nucleosome Mapping, High-Resolution Histone ChIP, and NCAM
    Article Snippet: .. Overnight saturated culture of S. cerevisiae strain 37% Formaldehyde 2.5 M Glycine Spheroblast solution (see recipe) β-mercaptoethanol (14.3 M) 100T zymolyase (AMSBIO) Micrococcal Nuclease (Worthington): Stored −80°C, 20U/μL in 10 mM Tris pH 7.4 Exonuclease III (NEB) MNase Digestion Buffer (See Recipe) MNase Stop Buffer (See Recipe) Proteinase K (20 mg/ml) 37°C incubator/water bath 65°C incubator Phenol:Chloroform:Isoamyl Alcohol (25:24:1) Ethanol (100%) 3M Sodium Acetate pH 5.2 Glycogen (20 mg/ml) NEB Buffer 2 RNase A (DNase-Free, 10 mg/ml) PCR Clean-up Kit (Qiagen) Alkaline Phosphatase (NEB) NEB Buffer 3 Low-melt Agarose (GeneMate) Gel Extraction Kit (Qiagen) TruSeq Sample Prep Kit (Illumina) [ or other desired library preparation kit ] MinElute PCR Purification Kit (Qiagen) Thermal Cycler for PCR Amplification ..

    Chromatin Immunoprecipitation:

    Article Title: Natural Single-Nucleosome Epi-Polymorphisms in Yeast
    Article Snippet: .. We followed the protocol of Liu et al. for both nucleosomal DNA isolation and ChIP, except that incubation time with micrococcal nuclease (Worthington Biochemical) prior to immunopurification was increased to 30 min at 37°C to obtain mononucleosomes. .. ChIP was performed using 3 µl of anti-H3K14Ac polyclonal antibody (Upstate, 07–353).

    Immu-Puri:

    Article Title: Natural Single-Nucleosome Epi-Polymorphisms in Yeast
    Article Snippet: .. We followed the protocol of Liu et al. for both nucleosomal DNA isolation and ChIP, except that incubation time with micrococcal nuclease (Worthington Biochemical) prior to immunopurification was increased to 30 min at 37°C to obtain mononucleosomes. .. ChIP was performed using 3 µl of anti-H3K14Ac polyclonal antibody (Upstate, 07–353).

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    Worthington Biochemical gst tagged hdac proteins
    AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various <t>HDAC</t> proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to <t>GST.</t> HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .
    Gst Tagged Hdac Proteins, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gst tagged hdac proteins/product/Worthington Biochemical
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gst tagged hdac proteins - by Bioz Stars, 2020-10
    90/100 stars
      Buy from Supplier

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    AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various HDAC proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to GST. HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .

    Journal: Nucleic Acids Research

    Article Title: Telomere-binding protein regulates the chromosome ends through the interaction with histone deacetylases in Arabidopsis thaliana

    doi: 10.1093/nar/gkw067

    Figure Lengend Snippet: AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various HDAC proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to GST. HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .

    Article Snippet: In vitro histone binding assay A total of 1–5 μg of GST and GST-tagged HDAC proteins were incubated with 1–5 μg of calf thymus total histones (Worthington) in binding buffer (50 mM Tris–HCl, pH 7.5, 1M NaCl, 1% NP-40, 0.5 mM EDTA, 1 mM phenylmethyl sulphonyl fluoride (PMSF) with protease inhibitors (Roche)) at 4°C for 3 h, followed by an additional incubation for 1 h with glutathione beads (GE Healthcare), as described previously ( ).

    Techniques: Histone Deacetylase Assay, Western Blot, Polyacrylamide Gel Electrophoresis, Purification, Pull Down Assay, Binding Assay, In Vivo, Chromatin Immunoprecipitation