gst tagged hdac proteins (Worthington Biochemical)


Name:
Nuclease Micrococcal
Description:
Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically SDS PAGE A lyophilized powder
Catalog Number:
ls004796
Price:
Inquire
Size:
Bulk
Source:
Staphylococcus aureus (Strain Foggi)
Cas Number:
9013.53.0
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Structured Review

Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically SDS PAGE A lyophilized powder
https://www.bioz.com/result/gst tagged hdac proteins/product/Worthington Biochemical
Average 88 stars, based on 160 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "Telomere-binding protein regulates the chromosome ends through the interaction with histone deacetylases in Arabidopsis thaliana"
Article Title: Telomere-binding protein regulates the chromosome ends through the interaction with histone deacetylases in Arabidopsis thaliana
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkw067

Figure Legend Snippet: AtTRB2 also interacts with HDA6, a RPD3-type histone deacetylase. ( A ) Western blot analysis showing the different patterns of interaction between AtTRB proteins and various HDAC proteins. PAGE shows the purified HDAC proteins used in this assay (first panel). ( B ) Scheme of various HDA6 truncated proteins fused to GST. HDA6 protein comprises three predicted regions: a conserved HDAC domain in the N-terminal region, a glutamate-rich region in the center of the protein and an aspartate-rich region in the C-terminal region. ( C ) Pull-down assay to map the regions of HDA6 that interact with AtTRB2. PAGE shows the purified HDA6-truncated proteins shown in (B). ( D ) Schematic map indicating the region of HDA6 involved in binding to AtTRB2. ( E ) Western blots demonstrating the interaction between AtTRB2 and HDA6 in vivo . Empty control and IP with IgG were used as negative controls. ( F ) HDA6-associated telomeric repeat signal was detected in an AtTRB2-dependent manner. ( G ) Quantification of multiple independent T-ChIP experiments as shown in (F). T-ChiP analysis was performed and values were calculated as described in the legend to Figure 4 .
Techniques Used: Histone Deacetylase Assay, Western Blot, Polyacrylamide Gel Electrophoresis, Purification, Pull Down Assay, Binding Assay, In Vivo, Chromatin Immunoprecipitation
2) Product Images from "The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins"
Article Title: The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins
Journal: Frontiers in Cell and Developmental Biology
doi: 10.3389/fcell.2020.00331

Figure Legend Snippet: Visualization of nuclei at different steps of the protocol using a phase-contrast microscope. Isolated nuclei at Step 6 (A) MNase-digested nuclei at Step 9 (B) TE-resuspended nuclei at Step 11 (C) .
Techniques Used: Microscopy, Isolation
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