human calprotectin  (Hycult Biotech)

Journal: Journal of Inflammation (London, England)

Article Title: Association of intestinal and systemic inflammatory biomarkers with immune reconstitution in HIV+ patients on ART

doi: 10.1186/s12950-020-00262-4

Figure Lengend Snippet: Systemic immune activation, inflammation, microbial translocation, proinflammatory cytokines, intestinal damage and inflammation biomarkers

Article Snippet: Also, fecal levels of secretory IgA (sIgA), calprotectin and lactoferrin were determined by ELISA, employing the following kit: Secretory IgA ELISA, sIgA (ALPCO, Salem, NH, USA), Human Lactoferrin and Human Calprotectin (both Hycult Biotech, Uden, the Netherlands) according to manufacturer instructions.

Techniques: Activation Assay, Translocation Assay, Expressing

Journal: Journal of Inflammation (London, England)

Article Title: Association of intestinal and systemic inflammatory biomarkers with immune reconstitution in HIV+ patients on ART

doi: 10.1186/s12950-020-00262-4

Figure Lengend Snippet: Determination of gut damage and inflammation. a I-FABP, b sST2, c Fecal lactoferrin, d Fecal calprotectin, e sIgA, f Association between I-FABP levels and Lipopolysaccharide concentration, g Association between fecal calprotectin levels and absolute CD4 + T-cells count, h Association between fecal calprotectin levels and duration of HIV infection and i Association between fecal calprotectin levels and duration of antiretroviral treatment. Kruskall-Wallis test with Bonferroni correction. Data show as median (IQR); * p < 0.05 . Spearman correlation test

Article Snippet: Also, fecal levels of secretory IgA (sIgA), calprotectin and lactoferrin were determined by ELISA, employing the following kit: Secretory IgA ELISA, sIgA (ALPCO, Salem, NH, USA), Human Lactoferrin and Human Calprotectin (both Hycult Biotech, Uden, the Netherlands) according to manufacturer instructions.

Techniques: Concentration Assay, Infection

Journal: Journal of Innate Immunity

Article Title: Induction of Myeloid-Derived Suppressor Cells in Cryopyrin-Associated Periodic Syndromes

doi: 10.1159/000446615

Figure Lengend Snippet: Inflammasome-dependent IL-1 family cytokines and alarmins induce MDSCs in vitro. a IL-1 family members IL-1β, Il-1α, and IL-18 are able to induce MDSCs. PBMCs were isolated from heparinized fresh blood from healthy donors using Ficoll density gradient sedimentation. MDSCs were induced by incubating PBMCs (5 × 105/ml) with complete medium only (medium control), 10 ng/ml GM-CSF (positive control), or different concentrations (1 ng/ml to 1 µg/ml) of the respective IL-1 family cytokines. MDSCs were determined as SSChighCD33+CD14- cells. The number of MDSCs as a percentage of all cells in medium-only cultures (mean 1.69%; median 1.3%) were set to 1-fold for every single experiment. The MDSC induction due to specific stimuli is presented as x-fold compared to medium control (mean ± SEM), and differences compared to controls were analyzed by a one-sample t test. b S100 proteins are able to slightly induce MDSCs. General culture conditions were as described under a. S100 proteins S100A7, S100A8, S100A9, or S100A12 were added in different concentrations ranging from 1 ng/ml to 1 µg/ml. The x-fold induction of MDSCs compared to medium control conditions is depicted as means ± SEM, and differences were analyzed by a one-sample t test. c Calprotectin is able to induce MDSCs. General culture conditions were as described under a. Calprotectin was added in different concentrations ranging from 1 ng/ml to 2 µg/ml. The x-fold induction of MDSCs compared to medium control conditions is depicted as means ± SEM, and differences were analyzed by a one-sample t test. d SAA is able to induce MDSCs. General culture conditions were as described in a. Recombinant hybrid SAA was added in different concentrations ranging from 0.1 ng/ml to 2 µg/ml. The x-fold induction of MDSCs compared to medium control conditions is depicted as means ± SEM, and differences were analyzed by a one-sample t test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Isolated human PBMCs from healthy controls were cultured in 12-well flat bottom plates (Corning) or 25-cm 2 flasks (Greiner Bio-One) at 5 × 10 5 cells/ml in RPMI 1640 supplemented with 10% heat-inactivated FCS (PAA Laboratories), 2 m M glutamine (Sigma-Aldrich), 100 IU/ml penicillin, and 100 mg/ml streptomycin (Biochrom) (subsequently referred to as ‘complete medium’) for 6 days, and granulocyte-macrophage colony-stimulating factor (GM-CSF, 10 ng/ml; Genzyme), IL-1β, IL-1α (both PeproTech), IL-18 (MBL International), IL-33 (GenWay Biotech), S100 proteins (ProSpec), calprotectin (Hycult Biotech), and hybrid serum amyloid A (SAA; PeproTech) were added at different concentrations as indicated in the respective figures.

Techniques: In Vitro, Isolation, Sedimentation, Positive Control, Recombinant