la muestra con una concentración alta de calprotectina se obtuvo agregándole calprotectina recombinante humana  (Hycult Biotech)

Journal: Advances in Laboratory Medicine

Article Title: Verification of fCAL turbo Buhlmann reagent for determining serum calprotectin

doi: 10.1515/almed-2024-0201

Figure Lengend Snippet: Bland-Altman plot for method comparison shows absolute bias for comparison of the Buhlmann fCAL turbo and GCAL – Gentian Serum Calprotectin. The blue solid line represents absolute bias with a 95 % confidence interval (Cl) as a green error bar.

Article Snippet: The sample with a high calprotectin concentration was obtained by spiking it with human recombinant calprotectin (ref. no. HC2120, HycultBiotech Inc., Wayne, USA).

Techniques: Comparison

Journal: Advances in Laboratory Medicine

Article Title: Verification of fCAL turbo Buhlmann reagent for determining serum calprotectin

doi: 10.1515/almed-2024-0201

Figure Lengend Snippet: Passing-Bablok regression of two measurements by reagents Buhlmann fCAL turbo and GCAL – Gentian Serum Calprotectin. On the x-axis are the measurements obtained by Buhlmann reagent, and on the y-axis are the measurements obtained by Gentian reagent. The blue solid line is a regression line with 95 %Cl, which is shown as red dashed lines.

Article Snippet: The sample with a high calprotectin concentration was obtained by spiking it with human recombinant calprotectin (ref. no. HC2120, HycultBiotech Inc., Wayne, USA).

Techniques:

Journal: Advances in Laboratory Medicine

Article Title: Verification of fCAL turbo Buhlmann reagent for determining serum calprotectin

doi: 10.1515/almed-2024-0201

Figure Lengend Snippet: Hook effect display in graphical form. The y-axis shows the concentration of calprotectin, and the x-axis shows the number of sample dilutions.

Article Snippet: The sample with a high calprotectin concentration was obtained by spiking it with human recombinant calprotectin (ref. no. HC2120, HycultBiotech Inc., Wayne, USA).

Techniques: Concentration Assay

Journal: Advances in Laboratory Medicine

Article Title: Verification of fCAL turbo Buhlmann reagent for determining serum calprotectin

doi: 10.1515/almed-2024-0201

Figure Lengend Snippet: Bland-Altman plot for method comparison shows absolute bias for comparison of the Buhlmann fCAL turbo and GCAL – Gentian Serum Calprotectin. The blue solid line represents absolute bias with a 95 % confidence interval (Cl) as a green error bar.

Article Snippet: The sample with a high calprotectin concentration was obtained by spiking it with human recombinant calprotectin (ref. no. HC2120, HycultBiotech Inc., Wayne, USA).

Techniques: Comparison

Journal: Advances in Laboratory Medicine

Article Title: Verification of fCAL turbo Buhlmann reagent for determining serum calprotectin

doi: 10.1515/almed-2024-0201

Figure Lengend Snippet: Passing-Bablok regression of two measurements by reagents Buhlmann fCAL turbo and GCAL – Gentian Serum Calprotectin. On the x-axis are the measurements obtained by Buhlmann reagent, and on the y-axis are the measurements obtained by Gentian reagent. The blue solid line is a regression line with 95 %Cl, which is shown as red dashed lines.

Article Snippet: The sample with a high calprotectin concentration was obtained by spiking it with human recombinant calprotectin (ref. no. HC2120, HycultBiotech Inc., Wayne, USA).

Techniques:

Journal: Advances in Laboratory Medicine

Article Title: Verification of fCAL turbo Buhlmann reagent for determining serum calprotectin

doi: 10.1515/almed-2024-0201

Figure Lengend Snippet: Hook effect display in graphical form. The y-axis shows the concentration of calprotectin, and the x-axis shows the number of sample dilutions.

Article Snippet: The sample with a high calprotectin concentration was obtained by spiking it with human recombinant calprotectin (ref. no. HC2120, HycultBiotech Inc., Wayne, USA).

Techniques: Concentration Assay

Journal: Journal of Inflammation (London, England)

Article Title: Association of intestinal and systemic inflammatory biomarkers with immune reconstitution in HIV+ patients on ART

doi: 10.1186/s12950-020-00262-4

Figure Lengend Snippet: Systemic immune activation, inflammation, microbial translocation, proinflammatory cytokines, intestinal damage and inflammation biomarkers

Article Snippet: Also, fecal levels of secretory IgA (sIgA), calprotectin and lactoferrin were determined by ELISA, employing the following kit: Secretory IgA ELISA, sIgA (ALPCO, Salem, NH, USA), Human Lactoferrin and Human Calprotectin (both Hycult Biotech, Uden, the Netherlands) according to manufacturer instructions.

Techniques: Activation Assay, Translocation Assay, Expressing

Journal: Journal of Inflammation (London, England)

Article Title: Association of intestinal and systemic inflammatory biomarkers with immune reconstitution in HIV+ patients on ART

doi: 10.1186/s12950-020-00262-4

Figure Lengend Snippet: Determination of gut damage and inflammation. a I-FABP, b sST2, c Fecal lactoferrin, d Fecal calprotectin, e sIgA, f Association between I-FABP levels and Lipopolysaccharide concentration, g Association between fecal calprotectin levels and absolute CD4 + T-cells count, h Association between fecal calprotectin levels and duration of HIV infection and i Association between fecal calprotectin levels and duration of antiretroviral treatment. Kruskall-Wallis test with Bonferroni correction. Data show as median (IQR); * p < 0.05 . Spearman correlation test

Article Snippet: Also, fecal levels of secretory IgA (sIgA), calprotectin and lactoferrin were determined by ELISA, employing the following kit: Secretory IgA ELISA, sIgA (ALPCO, Salem, NH, USA), Human Lactoferrin and Human Calprotectin (both Hycult Biotech, Uden, the Netherlands) according to manufacturer instructions.

Techniques: Concentration Assay, Infection