Journal: Oncotarget
Article Title: A novel multiplex detection array revealed systemic complement activation in oral squamous cell carcinoma
doi: 10.18632/oncotarget.22963
Figure Lengend Snippet: ( A ) Serial dilutions of native, human C3a-desArg (black circle), recombinant, human C5a-desArg (black square) and native human sC5b-C9 (black triangle) depicted different assay performances for the simultaneous analysis of complement activation markers. The target antigens were only detected by respective coupled magnetic beads and not by control beads (grey). Limit of detection (orange) and assay cutoffs (red square) for each analyte are shown. ( B ) The concentration range of complement activation markers determined with the novel multiplex assay from a human serum pool (black square with dot) corresponded to previously reported ranges for C3a, C5a and sC5b-C9 single detection either from plasma (grey), serum (black) or unknown sample matrix (white), respectively [ – ].
Article Snippet: A mixture of serial 1:3 diluted human native C3a-desArg (197.5–0.001 ng/mL, cat. HC2127, Hycult Biotech, Beutelsbach, Germany), human recombinant C5a-desArg (28.1–0.004 ng/mL, Hycult Biotech, Beutelsbach, Germany) and human native sC5b-9 (66.6–0.0004 µg/mL, cat. A127, Complement Technology, Tyler, TX, USA) were used as standard curve.
Techniques: Recombinant, Activation Assay, Magnetic Beads, Concentration Assay, Multiplex Assay