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human recombinant c5a  (Hycult Biotech)


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    Structured Review

    Hycult Biotech human recombinant c5a
    Human Recombinant C5a, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant c5a/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    human recombinant c5a - by Bioz Stars, 2025-02
    94/100 stars

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    Hycult Biotech recombinant human c3a
    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3 in red) and <t>complement</t> <t>activation</t> (C3d in green) in tissues obtained from (A) living donor kidneys (n=8), (B) acute tubular necrosis (ATN) (n=6), (C) Banff IA (n=6), (D) Banff IB (n=6), (E) Banff IIA (n=6), (F) Banff IIB (n=6), and (G) chronic rejection (CR) (n=6). Nuclei were counterstained with DAPI.
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    Hycult Biotech hc2101
    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3 in red) and <t>complement</t> <t>activation</t> (C3d in green) in tissues obtained from (A) living donor kidneys (n=8), (B) acute tubular necrosis (ATN) (n=6), (C) Banff IA (n=6), (D) Banff IB (n=6), (E) Banff IIA (n=6), (F) Banff IIB (n=6), and (G) chronic rejection (CR) (n=6). Nuclei were counterstained with DAPI.
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    Hycult Biotech recombinant human rh c5a
    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3 in red) and <t>complement</t> <t>activation</t> (C3d in green) in tissues obtained from (A) living donor kidneys (n=8), (B) acute tubular necrosis (ATN) (n=6), (C) Banff IA (n=6), (D) Banff IB (n=6), (E) Banff IIA (n=6), (F) Banff IIB (n=6), and (G) chronic rejection (CR) (n=6). Nuclei were counterstained with DAPI.
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    Hycult Biotech human c5a
    Zinc affects degranulation and the number of bacteria but does not alter phagocytosis. Neutrophils were incubated with or without 10 µM zinc and ( a ) primed with 25 ng/mL GM-CSF, incubated with 5 µg/mL cytochalasin B, 10 nM <t>C5a,</t> immunolabeled and analyzed by flow cytometry; ( b ) incubated with E. coli bioparticles and analysis of the percentage of cells phagocyting was analyzed by flow cytometry; ( c ) incubated with alive E. coli at the multiplicity of infection 10:1 ( E. coli : neutrophils) for 3 h and the number of colonies of survived bacteria from the collected supernatants were counted. One-way ANOVA with post hoc ( a ) Sidak’s test, ( b ) Holm–Sidak’s test, ( c ) Kruskal–Wallis test. The means + SEM are shown; ( a ) n = 3, ( b ) n = 6, ( c ) n = 4; * ( p ≤ 0.05).
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    Hycult Biotech c5a
    Zinc affects degranulation and the number of bacteria but does not alter phagocytosis. Neutrophils were incubated with or without 10 µM zinc and ( a ) primed with 25 ng/mL GM-CSF, incubated with 5 µg/mL cytochalasin B, 10 nM <t>C5a,</t> immunolabeled and analyzed by flow cytometry; ( b ) incubated with E. coli bioparticles and analysis of the percentage of cells phagocyting was analyzed by flow cytometry; ( c ) incubated with alive E. coli at the multiplicity of infection 10:1 ( E. coli : neutrophils) for 3 h and the number of colonies of survived bacteria from the collected supernatants were counted. One-way ANOVA with post hoc ( a ) Sidak’s test, ( b ) Holm–Sidak’s test, ( c ) Kruskal–Wallis test. The means + SEM are shown; ( a ) n = 3, ( b ) n = 6, ( c ) n = 4; * ( p ≤ 0.05).
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    https://www.bioz.com/result/c5a/product/Hycult Biotech
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    94/100 stars
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    Image Search Results


    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3 in red) and complement activation (C3d in green) in tissues obtained from (A) living donor kidneys (n=8), (B) acute tubular necrosis (ATN) (n=6), (C) Banff IA (n=6), (D) Banff IB (n=6), (E) Banff IIA (n=6), (F) Banff IIB (n=6), and (G) chronic rejection (CR) (n=6). Nuclei were counterstained with DAPI.

    Journal: medRxiv

    Article Title: Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation

    doi: 10.1101/2023.10.24.23297478

    Figure Lengend Snippet: Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3 in red) and complement activation (C3d in green) in tissues obtained from (A) living donor kidneys (n=8), (B) acute tubular necrosis (ATN) (n=6), (C) Banff IA (n=6), (D) Banff IB (n=6), (E) Banff IIA (n=6), (F) Banff IIB (n=6), and (G) chronic rejection (CR) (n=6). Nuclei were counterstained with DAPI.

    Article Snippet: In the experiments, we used recombinant human C3a (Hycult Biotech), recombinant human C5a (Hycult Biotech), and a combination of the two to stimulate the podocytes.

    Techniques: Double Staining, Activation Assay

    Different staining patterns of factor H-related protein 3 (FHR-3 in red) and complement activation (C3d in green) in transplanted kidneys. Nuclei were counterstained with DAPI. Representative images of (A) tubular FHR-3 staining (arrows) in acute tubular necrosis (ATN), (B) vascular FHR-3 staining (arrows) in T-cell-mediated rejection with severe tubulitis (Banff IB), and (C) low-intensity FHR-3 staining in Bowman’s capsule (arrows) and tubules (arrows) in chronic rejection (CR). Exemplary images of double staining for FHR-3 (red) and C3d (green) in transplanted kidneys revealing (C) predominant positive glomerular staining for FHR-3, (D) glomerular co-localization of FHR-3 and C3d, and (E) predominant positive glomerular staining for C3d.

    Journal: medRxiv

    Article Title: Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation

    doi: 10.1101/2023.10.24.23297478

    Figure Lengend Snippet: Different staining patterns of factor H-related protein 3 (FHR-3 in red) and complement activation (C3d in green) in transplanted kidneys. Nuclei were counterstained with DAPI. Representative images of (A) tubular FHR-3 staining (arrows) in acute tubular necrosis (ATN), (B) vascular FHR-3 staining (arrows) in T-cell-mediated rejection with severe tubulitis (Banff IB), and (C) low-intensity FHR-3 staining in Bowman’s capsule (arrows) and tubules (arrows) in chronic rejection (CR). Exemplary images of double staining for FHR-3 (red) and C3d (green) in transplanted kidneys revealing (C) predominant positive glomerular staining for FHR-3, (D) glomerular co-localization of FHR-3 and C3d, and (E) predominant positive glomerular staining for C3d.

    Article Snippet: In the experiments, we used recombinant human C3a (Hycult Biotech), recombinant human C5a (Hycult Biotech), and a combination of the two to stimulate the podocytes.

    Techniques: Staining, Activation Assay, Double Staining

    (A) Quantification of staining intensity of factor H-related protein 3 (FHR-3) and complement activation (C3d) by double immunofluorescent in living donor kidneys, acute tubular necrosis (ATN), Banff IA, Banff IB, Banff IIA, Banff IIB, and chronic rejection (CR). Each biopsy was scored by two independent observers in a blinded fashion. (B) The correlation between the staining intensity of FHR-3 and C3d using the Spearman Rank correlation coefficient (r represents the spearman’s rho). The dashed blue lines show the 95% confidence interval for the regression line (blue).

    Journal: medRxiv

    Article Title: Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation

    doi: 10.1101/2023.10.24.23297478

    Figure Lengend Snippet: (A) Quantification of staining intensity of factor H-related protein 3 (FHR-3) and complement activation (C3d) by double immunofluorescent in living donor kidneys, acute tubular necrosis (ATN), Banff IA, Banff IB, Banff IIA, Banff IIB, and chronic rejection (CR). Each biopsy was scored by two independent observers in a blinded fashion. (B) The correlation between the staining intensity of FHR-3 and C3d using the Spearman Rank correlation coefficient (r represents the spearman’s rho). The dashed blue lines show the 95% confidence interval for the regression line (blue).

    Article Snippet: In the experiments, we used recombinant human C3a (Hycult Biotech), recombinant human C5a (Hycult Biotech), and a combination of the two to stimulate the podocytes.

    Techniques: Staining, Activation Assay

    Zinc affects degranulation and the number of bacteria but does not alter phagocytosis. Neutrophils were incubated with or without 10 µM zinc and ( a ) primed with 25 ng/mL GM-CSF, incubated with 5 µg/mL cytochalasin B, 10 nM C5a, immunolabeled and analyzed by flow cytometry; ( b ) incubated with E. coli bioparticles and analysis of the percentage of cells phagocyting was analyzed by flow cytometry; ( c ) incubated with alive E. coli at the multiplicity of infection 10:1 ( E. coli : neutrophils) for 3 h and the number of colonies of survived bacteria from the collected supernatants were counted. One-way ANOVA with post hoc ( a ) Sidak’s test, ( b ) Holm–Sidak’s test, ( c ) Kruskal–Wallis test. The means + SEM are shown; ( a ) n = 3, ( b ) n = 6, ( c ) n = 4; * ( p ≤ 0.05).

    Journal: Nutrients

    Article Title: Zinc Supplementation Modulates NETs Release and Neutrophils’ Degranulation

    doi: 10.3390/nu13010051

    Figure Lengend Snippet: Zinc affects degranulation and the number of bacteria but does not alter phagocytosis. Neutrophils were incubated with or without 10 µM zinc and ( a ) primed with 25 ng/mL GM-CSF, incubated with 5 µg/mL cytochalasin B, 10 nM C5a, immunolabeled and analyzed by flow cytometry; ( b ) incubated with E. coli bioparticles and analysis of the percentage of cells phagocyting was analyzed by flow cytometry; ( c ) incubated with alive E. coli at the multiplicity of infection 10:1 ( E. coli : neutrophils) for 3 h and the number of colonies of survived bacteria from the collected supernatants were counted. One-way ANOVA with post hoc ( a ) Sidak’s test, ( b ) Holm–Sidak’s test, ( c ) Kruskal–Wallis test. The means + SEM are shown; ( a ) n = 3, ( b ) n = 6, ( c ) n = 4; * ( p ≤ 0.05).

    Article Snippet: To analyze neutrophils degranulation, 5 × 10 5 of human cells were primed with 25 ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF; Ref. no: 300-03, Peprotech, London, UK) for 15 min, incubated with 5 µg/mL cytochalasin B from Drechslera dematioidea (Ref. no: C6267, Sigma-Aldrich, St. Louis, MO, USA) for 5 min, followed by incubation with 10 nM of human C5a (Ref. no: HC2101, Hycult Biotech, Uden, The Netherlands) for 15 min. After this, the reaction was stopped with ice-cold PBS.

    Techniques: Incubation, Immunolabeling, Flow Cytometry, Infection

    Zinc inhibits NETs release in neutrophils isolated from mice. Mice were sacrificed, and bone marrow neutrophils were isolated. ( a , b ) Neutrophils were stimulated to release NETs with 4 µM CI, 10 µM PAF or 20 µg/mL LPS E. coli . NETs release was ( a ) measured fluorometrically and ( b ) analyzed by fluorescent microscopy. ( c ) Neutrophils were incubated with 4 µg/mL DHR 123 and stimulated with 100 nM PMA. ROS production was measured fluorometrically. ( d ) Cells were incubated with E. coli bioparticles, and the percentage of cells phagocyting bacteria was analyzed by flow cytometry. ( e ) Neutrophils were primed with GM-CSF, incubated with cytochalasin B, C5a and labeled with anti-CD63. Data are shown as means ( a , d , e ) + SEM; (c) ± SEM; ( a , e ) with individual values. One-way ANOVA with post hoc ( a ) Bonferroni’s test, ( d ) Holm–Sidak’s test; ( e ) Dunn’s test. Two-way ANOVA with post hoc ( c ) Dunnett’s test. ( a , b ) n = 6, ( c ) n = 4, ( d ) n = 7, ( e ) n = 5; * ( p ≤ 0.05), ** ( p ≤ 0.01), **** ( p ≤ 0.0001). NE: neutrophil elastase.

    Journal: Nutrients

    Article Title: Zinc Supplementation Modulates NETs Release and Neutrophils’ Degranulation

    doi: 10.3390/nu13010051

    Figure Lengend Snippet: Zinc inhibits NETs release in neutrophils isolated from mice. Mice were sacrificed, and bone marrow neutrophils were isolated. ( a , b ) Neutrophils were stimulated to release NETs with 4 µM CI, 10 µM PAF or 20 µg/mL LPS E. coli . NETs release was ( a ) measured fluorometrically and ( b ) analyzed by fluorescent microscopy. ( c ) Neutrophils were incubated with 4 µg/mL DHR 123 and stimulated with 100 nM PMA. ROS production was measured fluorometrically. ( d ) Cells were incubated with E. coli bioparticles, and the percentage of cells phagocyting bacteria was analyzed by flow cytometry. ( e ) Neutrophils were primed with GM-CSF, incubated with cytochalasin B, C5a and labeled with anti-CD63. Data are shown as means ( a , d , e ) + SEM; (c) ± SEM; ( a , e ) with individual values. One-way ANOVA with post hoc ( a ) Bonferroni’s test, ( d ) Holm–Sidak’s test; ( e ) Dunn’s test. Two-way ANOVA with post hoc ( c ) Dunnett’s test. ( a , b ) n = 6, ( c ) n = 4, ( d ) n = 7, ( e ) n = 5; * ( p ≤ 0.05), ** ( p ≤ 0.01), **** ( p ≤ 0.0001). NE: neutrophil elastase.

    Article Snippet: To analyze neutrophils degranulation, 5 × 10 5 of human cells were primed with 25 ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF; Ref. no: 300-03, Peprotech, London, UK) for 15 min, incubated with 5 µg/mL cytochalasin B from Drechslera dematioidea (Ref. no: C6267, Sigma-Aldrich, St. Louis, MO, USA) for 5 min, followed by incubation with 10 nM of human C5a (Ref. no: HC2101, Hycult Biotech, Uden, The Netherlands) for 15 min. After this, the reaction was stopped with ice-cold PBS.

    Techniques: Isolation, Microscopy, Incubation, Flow Cytometry, Labeling