cao m  (Worthington Biochemical)


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  • 88
    Name:
    Pepsin A
    Description:
    Two times crystallized from dilute alcohol A lyophilized powder
    Catalog Number:
    ls003317
    Price:
    412
    Size:
    10 gm
    Source:
    Porcine Stomach
    Cas Number:
    9001.75.6
    Buy from Supplier


    Structured Review

    Worthington Biochemical cao m
    Two times crystallized from dilute alcohol A lyophilized powder
    https://www.bioz.com/result/cao m/product/Worthington Biochemical
    Average 88 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
    cao m - by Bioz Stars, 2021-01
    88/100 stars

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    Related Articles

    Acetylene Reduction Assay:

    Article Title: Evaluating Disulfide Crosslinking and pH-induced Aggregation of Arachis hypogea 1 as Components of Peanut Allergy
    Article Snippet: .. Ara h 1 (WT or IAA-modified) was incubated in 0.2 M hydrochloric acid (HCl)-potassium chloride (KCl) pH 2 at 4 °C for 1 hour, followed by the addition of pepsin A (Worthington Biochemical Corp., Lakewood, NJ) to give 1/200 pepsin units/μg Ara h 1. ..

    Picogreen Assay:

    Article Title: The Effects of Hydroxyapatite Nanoparticles Embedded in a MMP-sensitive Photoclickable PEG Hydrogel on Encapsulated MC3T3-E1 Pre-Osteoblasts
    Article Snippet: .. Hydrogel lysates were assessed for total DNA content with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) and measured at an excitation of 485 nm and emission of 520 nm, alkaline phosphatase activity by enzyme cleavage of p-nitrophenol phosphate and measuring absorbance at 450 nm and total collagen content by digesting the samples with pepsin powder (Worthington) in 0.5M acetic acid overnight and incubated with Sirius Red (Sigma) and measuring absorbance at 544 nm. ..

    Purification:

    Article Title: Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
    Article Snippet: .. Protease treatments Purified enzyme (1.5 µg) was incubated with the same amount of pepsin A (Worthington) (1.5 µg) in 0.1 M Gly-HCl (pH 2.0) or 1:1 mixture of the trypsin (Sigma-Aldrich) and chymotrypsin (Sigma-Aldrich) (1.5 µg) based on mass concentration in 0.1 M Tris-HCl (pH 7.6) at 37 °C for 0, 10, 40 and 60 min. After incubation, protease inhibitors were added and acid conditions were neutralized by addition of 1 M Tris-HCl (pH 7.6). ..

    Article Title: Residues of acidic chitinase cause chitinolytic activity degrading chitosan in porcine pepsin preparations
    Article Snippet: .. This being a contra-intuitive hypothesis, we first explored the entity of this activity by exposing chitosan (degree of deacetylation, DD 80%) as well as α- or β-crystalline chitin (DD 2% or 10%, respectively) to a pepsin preparation (Sigma-Aldrich, P7012) or purified pepsin A (Pep A, Worthington) at pH 2.0 or pH 4.0. .. The resulting oligosaccharides were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE) as described in Methods.

    Concentration Assay:

    Article Title: Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
    Article Snippet: .. Protease treatments Purified enzyme (1.5 µg) was incubated with the same amount of pepsin A (Worthington) (1.5 µg) in 0.1 M Gly-HCl (pH 2.0) or 1:1 mixture of the trypsin (Sigma-Aldrich) and chymotrypsin (Sigma-Aldrich) (1.5 µg) based on mass concentration in 0.1 M Tris-HCl (pH 7.6) at 37 °C for 0, 10, 40 and 60 min. After incubation, protease inhibitors were added and acid conditions were neutralized by addition of 1 M Tris-HCl (pH 7.6). ..

    Incubation:

    Article Title: The Effects of Hydroxyapatite Nanoparticles Embedded in a MMP-sensitive Photoclickable PEG Hydrogel on Encapsulated MC3T3-E1 Pre-Osteoblasts
    Article Snippet: .. Hydrogel lysates were assessed for total DNA content with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) and measured at an excitation of 485 nm and emission of 520 nm, alkaline phosphatase activity by enzyme cleavage of p-nitrophenol phosphate and measuring absorbance at 450 nm and total collagen content by digesting the samples with pepsin powder (Worthington) in 0.5M acetic acid overnight and incubated with Sirius Red (Sigma) and measuring absorbance at 544 nm. ..

    Article Title: Structural and dynamic properties of allergen and non-allergen forms of tropomyosin
    Article Snippet: .. Full length tropomyosin (shrimp or pig) was incubated for one hour at 37 °C in acidic buffer [0.2 M HCl−potassium chloride (KCl), pH 2], followed by the addition of pepsin A (Worthington Biochemical Corp., Lakewood, NJ) to give 0.33 Units of pepsin activity/μg tropomyosin substrate. ..

    Article Title: Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
    Article Snippet: .. Protease treatments Purified enzyme (1.5 µg) was incubated with the same amount of pepsin A (Worthington) (1.5 µg) in 0.1 M Gly-HCl (pH 2.0) or 1:1 mixture of the trypsin (Sigma-Aldrich) and chymotrypsin (Sigma-Aldrich) (1.5 µg) based on mass concentration in 0.1 M Tris-HCl (pH 7.6) at 37 °C for 0, 10, 40 and 60 min. After incubation, protease inhibitors were added and acid conditions were neutralized by addition of 1 M Tris-HCl (pH 7.6). ..

    Article Title: Evaluating Disulfide Crosslinking and pH-induced Aggregation of Arachis hypogea 1 as Components of Peanut Allergy
    Article Snippet: .. Ara h 1 (WT or IAA-modified) was incubated in 0.2 M hydrochloric acid (HCl)-potassium chloride (KCl) pH 2 at 4 °C for 1 hour, followed by the addition of pepsin A (Worthington Biochemical Corp., Lakewood, NJ) to give 1/200 pepsin units/μg Ara h 1. ..

    Activity Assay:

    Article Title: Evaluating Potential Risks of Food Allergy and Toxicity of Soy Leghemoglobin Expressed in Pichia pastoris
    Article Snippet: .. Pepsin A (Worthington Biochemicals, #3319) with a certificate of analysis of 2810 activity units per mg solid was used in all assays. .. Novex® 10–20% tris‐glycine polyacrylamide gels (Invitrogen) were used to separate digested materials.

    Article Title: The Effects of Hydroxyapatite Nanoparticles Embedded in a MMP-sensitive Photoclickable PEG Hydrogel on Encapsulated MC3T3-E1 Pre-Osteoblasts
    Article Snippet: .. Hydrogel lysates were assessed for total DNA content with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) and measured at an excitation of 485 nm and emission of 520 nm, alkaline phosphatase activity by enzyme cleavage of p-nitrophenol phosphate and measuring absorbance at 450 nm and total collagen content by digesting the samples with pepsin powder (Worthington) in 0.5M acetic acid overnight and incubated with Sirius Red (Sigma) and measuring absorbance at 544 nm. ..

    Article Title: Structural and dynamic properties of allergen and non-allergen forms of tropomyosin
    Article Snippet: .. Full length tropomyosin (shrimp or pig) was incubated for one hour at 37 °C in acidic buffer [0.2 M HCl−potassium chloride (KCl), pH 2], followed by the addition of pepsin A (Worthington Biochemical Corp., Lakewood, NJ) to give 0.33 Units of pepsin activity/μg tropomyosin substrate. ..

    Article Title: Residues of acidic chitinase cause chitinolytic activity degrading chitosan in porcine pepsin preparations
    Article Snippet: .. This being a contra-intuitive hypothesis, we first explored the entity of this activity by exposing chitosan (degree of deacetylation, DD 80%) as well as α- or β-crystalline chitin (DD 2% or 10%, respectively) to a pepsin preparation (Sigma-Aldrich, P7012) or purified pepsin A (Pep A, Worthington) at pH 2.0 or pH 4.0. .. The resulting oligosaccharides were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE) as described in Methods.

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