h 2 o 2  (Worthington Biochemical)


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    Worthington Biochemical h 2 o 2
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    H 2 O 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction"

    Article Title: In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0055548

    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    Figure Legend Snippet: Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.

    Techniques Used:


    Figure Legend Snippet: Measurement of the diameters of the blue holes.

    Techniques Used:

    The distribution can perfectly be fitted with the exponential equation y = 0.673× 5.611 , where x is the diameter of blue hole and y the H 2 O 2 concentration. The inset showed that in the range of 0.5 mM≤H 2 O 2 ≤20 mM, the change in diameter of blue hole was a function of logarithm of H 2 O 2 concentration with linear fits under the equation y = 1.772x–1.890, where x is the diameter of blue hole and y the logarithm of H 2 O 2 concentration.
    Figure Legend Snippet: The distribution can perfectly be fitted with the exponential equation y = 0.673× 5.611 , where x is the diameter of blue hole and y the H 2 O 2 concentration. The inset showed that in the range of 0.5 mM≤H 2 O 2 ≤20 mM, the change in diameter of blue hole was a function of logarithm of H 2 O 2 concentration with linear fits under the equation y = 1.772x–1.890, where x is the diameter of blue hole and y the logarithm of H 2 O 2 concentration.

    Techniques Used: Concentration Assay

    On the basis of the diameters of the formed Prussian blue, the concentrations of H 2 O 2 produced by LAAO activities were calculated by using the equations in . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Prussian blue agar assay for detection of LAAO activity. The diameters of the blue holes were indicated under the holes.
    Figure Legend Snippet: On the basis of the diameters of the formed Prussian blue, the concentrations of H 2 O 2 produced by LAAO activities were calculated by using the equations in . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Prussian blue agar assay for detection of LAAO activity. The diameters of the blue holes were indicated under the holes.

    Techniques Used: Produced, Activity Assay

    For Prussian blue agar assay, standard H 2 O 2 solutions with different concentrations ranging from 0.5 mM to 20 mM were used as the positive controls; as the negative controls, CK1 and CK2 were the R3 culture supernatant without any substrate for oxidization. 2, 4-dinitrophenylhydrazine (DNP) method was applied to detecting α-keto acids derived from their corresponding L-amino acids by LAAO activity.
    Figure Legend Snippet: For Prussian blue agar assay, standard H 2 O 2 solutions with different concentrations ranging from 0.5 mM to 20 mM were used as the positive controls; as the negative controls, CK1 and CK2 were the R3 culture supernatant without any substrate for oxidization. 2, 4-dinitrophenylhydrazine (DNP) method was applied to detecting α-keto acids derived from their corresponding L-amino acids by LAAO activity.

    Techniques Used: Derivative Assay, Activity Assay

    h 2 o 2  (Worthington Biochemical)


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    Worthington Biochemical h 2 o 2
    Effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were incubated for 24 h with 0 μ M, 20 μ M, 100 μ M, or 500 μ M of Vit C and then exposed to 100 μ M of H 2 O 2 for 12 h or 24 h. Viability, apoptosis, and intracellular ROS were measured by using MTT assays, TUNEL assays, and DCFH-DA, respectively, as shown in . Normal ARPE-19 cells which did not undergo treatment were expressed as “control.” Data were expressed as a percentage of cell survival (a), apoptosis (b), and relative fluorescence intensity (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.
    H 2 O 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    h 2 o 2 - by Bioz Stars, 2023-03
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    1) Product Images from "Vitamin C Protected Human Retinal Pigmented Epithelium from Oxidant Injury Depending on Regulating SIRT1"

    Article Title: Vitamin C Protected Human Retinal Pigmented Epithelium from Oxidant Injury Depending on Regulating SIRT1

    Journal: The Scientific World Journal

    doi: 10.1155/2014/750634

    Effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were incubated for 24 h with 0 μ M, 20 μ M, 100 μ M, or 500 μ M of Vit C and then exposed to 100 μ M of H 2 O 2 for 12 h or 24 h. Viability, apoptosis, and intracellular ROS were measured by using MTT assays, TUNEL assays, and DCFH-DA, respectively, as shown in . Normal ARPE-19 cells which did not undergo treatment were expressed as “control.” Data were expressed as a percentage of cell survival (a), apoptosis (b), and relative fluorescence intensity (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.
    Figure Legend Snippet: Effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were incubated for 24 h with 0 μ M, 20 μ M, 100 μ M, or 500 μ M of Vit C and then exposed to 100 μ M of H 2 O 2 for 12 h or 24 h. Viability, apoptosis, and intracellular ROS were measured by using MTT assays, TUNEL assays, and DCFH-DA, respectively, as shown in . Normal ARPE-19 cells which did not undergo treatment were expressed as “control.” Data were expressed as a percentage of cell survival (a), apoptosis (b), and relative fluorescence intensity (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.

    Techniques Used: Incubation, TUNEL Assay, Fluorescence

    SIRT1 changed the effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were preincubated with RSV (10 mM) or NA (5 mM) for 4 h. Then, cells were exposed to H 2 O 2 for 12 h after treatments of indicated concentration of Vit C. Viability (a), apoptosis (b), and intracellular ROS (c) were measured and compared among different groups. N1, N2, N3, and N4 were standing for the cells treated with H 2 O 2 and without Vit C preincubation, cells treated with H 2 O 2 and 20 μ M Vit C, cells treated with H 2 O 2 and 100 μ M Vit C, and cells treated with H 2 O 2 and 500 μ M Vit C, respectively. Single treatment was performed in triplicate. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.
    Figure Legend Snippet: SIRT1 changed the effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were preincubated with RSV (10 mM) or NA (5 mM) for 4 h. Then, cells were exposed to H 2 O 2 for 12 h after treatments of indicated concentration of Vit C. Viability (a), apoptosis (b), and intracellular ROS (c) were measured and compared among different groups. N1, N2, N3, and N4 were standing for the cells treated with H 2 O 2 and without Vit C preincubation, cells treated with H 2 O 2 and 20 μ M Vit C, cells treated with H 2 O 2 and 100 μ M Vit C, and cells treated with H 2 O 2 and 500 μ M Vit C, respectively. Single treatment was performed in triplicate. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.

    Techniques Used: Concentration Assay

    Regulation of FoxO3 and p53 was involved in the expression of SIRT1 rather than Vit C. ARPE-19 cells underwent SIRT1 knockdown and overexpression of SIRT1 by using siRNA (SIRT1si group), pRC/CMV-SRIT1 (SIRT1up group), and its control (C-SIRT1si and C-SIRT1up groups, resp.). Then, cells were exposed to 100 μ M of H 2 O 2 for 12 h after being incubated for 24 h with 100 μ M of Vit C. The expression of p53 (a) and FOXO3 (b) mRNA was detected by qRT-PCR and was normalized by 2 −ΔΔCt method as relative quantification. The expression of SIRT1, p53, and FOXO3 proteins was assayed by western blot in ARPE-19 cells with indicated treatments (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statisticalsignificance was expressed as * P < 0.05 and ** P < 0.01.
    Figure Legend Snippet: Regulation of FoxO3 and p53 was involved in the expression of SIRT1 rather than Vit C. ARPE-19 cells underwent SIRT1 knockdown and overexpression of SIRT1 by using siRNA (SIRT1si group), pRC/CMV-SRIT1 (SIRT1up group), and its control (C-SIRT1si and C-SIRT1up groups, resp.). Then, cells were exposed to 100 μ M of H 2 O 2 for 12 h after being incubated for 24 h with 100 μ M of Vit C. The expression of p53 (a) and FOXO3 (b) mRNA was detected by qRT-PCR and was normalized by 2 −ΔΔCt method as relative quantification. The expression of SIRT1, p53, and FOXO3 proteins was assayed by western blot in ARPE-19 cells with indicated treatments (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statisticalsignificance was expressed as * P < 0.05 and ** P < 0.01.

    Techniques Used: Expressing, Over Expression, Incubation, Quantitative RT-PCR, Western Blot

    h 2 o 2  (Worthington Biochemical)


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    Worthington Biochemical h 2 o 2
    Effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were incubated for 24 h with 0 μ M, 20 μ M, 100 μ M, or 500 μ M of Vit C and then exposed to 100 μ M of H 2 O 2 for 12 h or 24 h. Viability, apoptosis, and intracellular ROS were measured by using MTT assays, TUNEL assays, and DCFH-DA, respectively, as shown in . Normal ARPE-19 cells which did not undergo treatment were expressed as “control.” Data were expressed as a percentage of cell survival (a), apoptosis (b), and relative fluorescence intensity (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.
    H 2 O 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h 2 o 2/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h 2 o 2 - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "Vitamin C Protected Human Retinal Pigmented Epithelium from Oxidant Injury Depending on Regulating SIRT1"

    Article Title: Vitamin C Protected Human Retinal Pigmented Epithelium from Oxidant Injury Depending on Regulating SIRT1

    Journal: The Scientific World Journal

    doi: 10.1155/2014/750634

    Effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were incubated for 24 h with 0 μ M, 20 μ M, 100 μ M, or 500 μ M of Vit C and then exposed to 100 μ M of H 2 O 2 for 12 h or 24 h. Viability, apoptosis, and intracellular ROS were measured by using MTT assays, TUNEL assays, and DCFH-DA, respectively, as shown in . Normal ARPE-19 cells which did not undergo treatment were expressed as “control.” Data were expressed as a percentage of cell survival (a), apoptosis (b), and relative fluorescence intensity (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.
    Figure Legend Snippet: Effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were incubated for 24 h with 0 μ M, 20 μ M, 100 μ M, or 500 μ M of Vit C and then exposed to 100 μ M of H 2 O 2 for 12 h or 24 h. Viability, apoptosis, and intracellular ROS were measured by using MTT assays, TUNEL assays, and DCFH-DA, respectively, as shown in . Normal ARPE-19 cells which did not undergo treatment were expressed as “control.” Data were expressed as a percentage of cell survival (a), apoptosis (b), and relative fluorescence intensity (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.

    Techniques Used: Incubation, TUNEL Assay, Fluorescence

    SIRT1 changed the effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were preincubated with RSV (10 mM) or NA (5 mM) for 4 h. Then, cells were exposed to H 2 O 2 for 12 h after treatments of indicated concentration of Vit C. Viability (a), apoptosis (b), and intracellular ROS (c) were measured and compared among different groups. N1, N2, N3, and N4 were standing for the cells treated with H 2 O 2 and without Vit C preincubation, cells treated with H 2 O 2 and 20 μ M Vit C, cells treated with H 2 O 2 and 100 μ M Vit C, and cells treated with H 2 O 2 and 500 μ M Vit C, respectively. Single treatment was performed in triplicate. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.
    Figure Legend Snippet: SIRT1 changed the effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H 2 O 2 . ARPE-19 cells were preincubated with RSV (10 mM) or NA (5 mM) for 4 h. Then, cells were exposed to H 2 O 2 for 12 h after treatments of indicated concentration of Vit C. Viability (a), apoptosis (b), and intracellular ROS (c) were measured and compared among different groups. N1, N2, N3, and N4 were standing for the cells treated with H 2 O 2 and without Vit C preincubation, cells treated with H 2 O 2 and 20 μ M Vit C, cells treated with H 2 O 2 and 100 μ M Vit C, and cells treated with H 2 O 2 and 500 μ M Vit C, respectively. Single treatment was performed in triplicate. Statistical significance was expressed as * P < 0.05 and ** P < 0.01.

    Techniques Used: Concentration Assay

    Regulation of FoxO3 and p53 was involved in the expression of SIRT1 rather than Vit C. ARPE-19 cells underwent SIRT1 knockdown and overexpression of SIRT1 by using siRNA (SIRT1si group), pRC/CMV-SRIT1 (SIRT1up group), and its control (C-SIRT1si and C-SIRT1up groups, resp.). Then, cells were exposed to 100 μ M of H 2 O 2 for 12 h after being incubated for 24 h with 100 μ M of Vit C. The expression of p53 (a) and FOXO3 (b) mRNA was detected by qRT-PCR and was normalized by 2 −ΔΔCt method as relative quantification. The expression of SIRT1, p53, and FOXO3 proteins was assayed by western blot in ARPE-19 cells with indicated treatments (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statisticalsignificance was expressed as * P < 0.05 and ** P < 0.01.
    Figure Legend Snippet: Regulation of FoxO3 and p53 was involved in the expression of SIRT1 rather than Vit C. ARPE-19 cells underwent SIRT1 knockdown and overexpression of SIRT1 by using siRNA (SIRT1si group), pRC/CMV-SRIT1 (SIRT1up group), and its control (C-SIRT1si and C-SIRT1up groups, resp.). Then, cells were exposed to 100 μ M of H 2 O 2 for 12 h after being incubated for 24 h with 100 μ M of Vit C. The expression of p53 (a) and FOXO3 (b) mRNA was detected by qRT-PCR and was normalized by 2 −ΔΔCt method as relative quantification. The expression of SIRT1, p53, and FOXO3 proteins was assayed by western blot in ARPE-19 cells with indicated treatments (c). Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statisticalsignificance was expressed as * P < 0.05 and ** P < 0.01.

    Techniques Used: Expressing, Over Expression, Incubation, Quantitative RT-PCR, Western Blot

    h 2 o 2  (Worthington Biochemical)


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    Worthington Biochemical h 2 o 2
    E. faecalis MN1 and L. crispatus ATCC 33197 were grown overnight in KSFM tissue culture medium. Hydrogen peroxide production was measured using a H 2 O 2 colorimetric detection assay kit.
    H 2 O 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    h 2 o 2 - by Bioz Stars, 2023-03
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    1) Product Images from "Enterococcus faecalis Inhibits Superantigen Toxic Shock Syndrome Toxin-1-Induced Interleukin-8 from Human Vaginal Epithelial Cells through Tetramic Acids"

    Article Title: Enterococcus faecalis Inhibits Superantigen Toxic Shock Syndrome Toxin-1-Induced Interleukin-8 from Human Vaginal Epithelial Cells through Tetramic Acids

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0061255

    E. faecalis MN1 and L. crispatus ATCC 33197 were grown overnight in KSFM tissue culture medium. Hydrogen peroxide production was measured using a H 2 O 2 colorimetric detection assay kit.
    Figure Legend Snippet: E. faecalis MN1 and L. crispatus ATCC 33197 were grown overnight in KSFM tissue culture medium. Hydrogen peroxide production was measured using a H 2 O 2 colorimetric detection assay kit.

    Techniques Used: Detection Assay

    h b concentrate  (Worthington Biochemical)


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    Worthington Biochemical h b concentrate
    H B Concentrate, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    h o methanol  (Worthington Biochemical)


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    Worthington Biochemical h o methanol
    H O Methanol, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mem h  (Worthington Biochemical)


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    Worthington Biochemical mem h
    Mem H, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nah 2 po 4 h 2 o  (Worthington Biochemical)


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    Worthington Biochemical nah 2 po 4 h 2 o
    Nah 2 Po 4 H 2 O, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hbss h  (Worthington Biochemical)


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    Worthington Biochemical hbss h
    Hbss H, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    h 2 o 2  (Worthington Biochemical)


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    Worthington Biochemical h 2 o 2
    H 2 O 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    • h 2 o 2  (Worthington Biochemical)
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    Worthington Biochemical h 2 o 2
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    H 2 O 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h 2 o 2/product/Worthington Biochemical
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    h b concentrate  (Worthington Biochemical)
    86
    Worthington Biochemical h b concentrate
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    H B Concentrate, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h b concentrate/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    h o methanol  (Worthington Biochemical)
    86
    Worthington Biochemical h o methanol
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    H O Methanol, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h o methanol/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h o methanol - by Bioz Stars, 2023-03
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    mem h  (Worthington Biochemical)
    86
    Worthington Biochemical mem h
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    Mem H, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mem h/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mem h - by Bioz Stars, 2023-03
    86/100 stars
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    nah 2 po 4 h 2 o  (Worthington Biochemical)
    86
    Worthington Biochemical nah 2 po 4 h 2 o
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    Nah 2 Po 4 H 2 O, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nah 2 po 4 h 2 o/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nah 2 po 4 h 2 o - by Bioz Stars, 2023-03
    86/100 stars
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    hbss h  (Worthington Biochemical)
    86
    Worthington Biochemical hbss h
    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.
    Hbss H, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss h/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbss h - by Bioz Stars, 2023-03
    86/100 stars
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    Image Search Results


    Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.

    Journal: PLoS ONE

    Article Title: In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

    doi: 10.1371/journal.pone.0055548

    Figure Lengend Snippet: Row 1 from left to right: pH 1 up to 14 of background solutions prepared by a mixture of 6 N HCl with 6 N NaOH; row 2: individual standard 5 mM H 2 O 2 solutions with uniform pH of 7.5; row 3 from left to right: individual standard 5 mM H 2 O 2 solutions with different pH values ranging from 1 to 14 adjusted by either HCl or NaOH; row 4: the detection of oxidization reactions of L-Leu by LAAO harvested from strain R3 with uniform pH of 7.5; row 5 from left to right: same as row 4, except that the pH values of reaction solutions were adjusted to 1 to 14 after oxidation. Prussian blue agar medium contained peptone and yeast extract in the upper three rows, but no peptone and yeast extract in the lower two rows.

    Article Snippet: To confirm the method with another enzyme source using a purified enzyme, the commercial Crotalus adamanteus venom LAAO (caLAAO, Worthington Biochemical Corporation, USA) was applied to Prussian blue assay with L-Leu as substrate. showed that caLAAO gave a blue hole with 1.51 cm diameter which corresponds to around 6.11 mM H 2 O 2 based on the extracted equation.

    Techniques:

    Journal: PLoS ONE

    Article Title: In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

    doi: 10.1371/journal.pone.0055548

    Figure Lengend Snippet: Measurement of the diameters of the blue holes.

    Article Snippet: To confirm the method with another enzyme source using a purified enzyme, the commercial Crotalus adamanteus venom LAAO (caLAAO, Worthington Biochemical Corporation, USA) was applied to Prussian blue assay with L-Leu as substrate. showed that caLAAO gave a blue hole with 1.51 cm diameter which corresponds to around 6.11 mM H 2 O 2 based on the extracted equation.

    Techniques:

    The distribution can perfectly be fitted with the exponential equation y = 0.673× 5.611 , where x is the diameter of blue hole and y the H 2 O 2 concentration. The inset showed that in the range of 0.5 mM≤H 2 O 2 ≤20 mM, the change in diameter of blue hole was a function of logarithm of H 2 O 2 concentration with linear fits under the equation y = 1.772x–1.890, where x is the diameter of blue hole and y the logarithm of H 2 O 2 concentration.

    Journal: PLoS ONE

    Article Title: In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

    doi: 10.1371/journal.pone.0055548

    Figure Lengend Snippet: The distribution can perfectly be fitted with the exponential equation y = 0.673× 5.611 , where x is the diameter of blue hole and y the H 2 O 2 concentration. The inset showed that in the range of 0.5 mM≤H 2 O 2 ≤20 mM, the change in diameter of blue hole was a function of logarithm of H 2 O 2 concentration with linear fits under the equation y = 1.772x–1.890, where x is the diameter of blue hole and y the logarithm of H 2 O 2 concentration.

    Article Snippet: To confirm the method with another enzyme source using a purified enzyme, the commercial Crotalus adamanteus venom LAAO (caLAAO, Worthington Biochemical Corporation, USA) was applied to Prussian blue assay with L-Leu as substrate. showed that caLAAO gave a blue hole with 1.51 cm diameter which corresponds to around 6.11 mM H 2 O 2 based on the extracted equation.

    Techniques: Concentration Assay

    On the basis of the diameters of the formed Prussian blue, the concentrations of H 2 O 2 produced by LAAO activities were calculated by using the equations in . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Prussian blue agar assay for detection of LAAO activity. The diameters of the blue holes were indicated under the holes.

    Journal: PLoS ONE

    Article Title: In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

    doi: 10.1371/journal.pone.0055548

    Figure Lengend Snippet: On the basis of the diameters of the formed Prussian blue, the concentrations of H 2 O 2 produced by LAAO activities were calculated by using the equations in . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Prussian blue agar assay for detection of LAAO activity. The diameters of the blue holes were indicated under the holes.

    Article Snippet: To confirm the method with another enzyme source using a purified enzyme, the commercial Crotalus adamanteus venom LAAO (caLAAO, Worthington Biochemical Corporation, USA) was applied to Prussian blue assay with L-Leu as substrate. showed that caLAAO gave a blue hole with 1.51 cm diameter which corresponds to around 6.11 mM H 2 O 2 based on the extracted equation.

    Techniques: Produced, Activity Assay

    For Prussian blue agar assay, standard H 2 O 2 solutions with different concentrations ranging from 0.5 mM to 20 mM were used as the positive controls; as the negative controls, CK1 and CK2 were the R3 culture supernatant without any substrate for oxidization. 2, 4-dinitrophenylhydrazine (DNP) method was applied to detecting α-keto acids derived from their corresponding L-amino acids by LAAO activity.

    Journal: PLoS ONE

    Article Title: In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

    doi: 10.1371/journal.pone.0055548

    Figure Lengend Snippet: For Prussian blue agar assay, standard H 2 O 2 solutions with different concentrations ranging from 0.5 mM to 20 mM were used as the positive controls; as the negative controls, CK1 and CK2 were the R3 culture supernatant without any substrate for oxidization. 2, 4-dinitrophenylhydrazine (DNP) method was applied to detecting α-keto acids derived from their corresponding L-amino acids by LAAO activity.

    Article Snippet: To confirm the method with another enzyme source using a purified enzyme, the commercial Crotalus adamanteus venom LAAO (caLAAO, Worthington Biochemical Corporation, USA) was applied to Prussian blue assay with L-Leu as substrate. showed that caLAAO gave a blue hole with 1.51 cm diameter which corresponds to around 6.11 mM H 2 O 2 based on the extracted equation.

    Techniques: Derivative Assay, Activity Assay