affinity nickel nta agarose resin  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc affinity nickel nta agarose resin
    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from <t>NTA-His</t> column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
    Affinity Nickel Nta Agarose Resin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity nickel nta agarose resin/product/Gold Biotechnology Inc
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    affinity nickel nta agarose resin - by Bioz Stars, 2022-09
    94/100 stars

    Images

    1) Product Images from "Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection"

    Article Title: Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection

    Journal: bioRxiv

    doi: 10.1101/2022.08.16.502937

    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
    Figure Legend Snippet: Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.

    Techniques Used: Expressing, SDS Page, Protein Purification, Marker, Purification, Inhibition, Activity Assay

    2) Product Images from "A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection"

    Article Title: A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection

    Journal: bioRxiv

    doi: 10.1101/2022.07.28.501919

    ELISA assay for detecting His-tagged proteins in TXTL. His Tag Elisa kit (LS-F55748) was purchased from LifeSpan Bioscience, inc. The cell-free preps were diluted to one-fifth with water, and the ELISA assay was performed following the manufacturer’s protocol. The standard curve was prepared using the standard sample prepared by the manufacturer. We confirmed the significant decrease of His-tagged protein in the Hina HI extract (After Ni-NTA treatment) compared to the Hina HI extract (Before Ni-NTA treatment. Error bars indicate SEM, n=3.
    Figure Legend Snippet: ELISA assay for detecting His-tagged proteins in TXTL. His Tag Elisa kit (LS-F55748) was purchased from LifeSpan Bioscience, inc. The cell-free preps were diluted to one-fifth with water, and the ELISA assay was performed following the manufacturer’s protocol. The standard curve was prepared using the standard sample prepared by the manufacturer. We confirmed the significant decrease of His-tagged protein in the Hina HI extract (After Ni-NTA treatment) compared to the Hina HI extract (Before Ni-NTA treatment. Error bars indicate SEM, n=3.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    3) Product Images from "A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection"

    Article Title: A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection

    Journal: bioRxiv

    doi: 10.1101/2022.07.28.501919

    ELISA assay for detecting His-tagged proteins in TXTL. His Tag Elisa kit (LS-F55748) was purchased from LifeSpan Bioscience, inc. The cell-free preps were diluted to one-fifth with water, and the ELISA assay was performed following the manufacturer’s protocol. The standard curve was prepared using the standard sample prepared by the manufacturer. We confirmed the significant decrease of His-tagged protein in the Hina HI extract (After Ni-NTA treatment) compared to the Hina HI extract (Before Ni-NTA treatment. Error bars indicate SEM, n=3.
    Figure Legend Snippet: ELISA assay for detecting His-tagged proteins in TXTL. His Tag Elisa kit (LS-F55748) was purchased from LifeSpan Bioscience, inc. The cell-free preps were diluted to one-fifth with water, and the ELISA assay was performed following the manufacturer’s protocol. The standard curve was prepared using the standard sample prepared by the manufacturer. We confirmed the significant decrease of His-tagged protein in the Hina HI extract (After Ni-NTA treatment) compared to the Hina HI extract (Before Ni-NTA treatment. Error bars indicate SEM, n=3.

    Techniques Used: Enzyme-linked Immunosorbent Assay

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  • News
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  • Team
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  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Gold Biotechnology Inc affinity nickel nta agarose resin
    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from <t>NTA-His</t> column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
    Affinity Nickel Nta Agarose Resin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity nickel nta agarose resin/product/Gold Biotechnology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity nickel nta agarose resin - by Bioz Stars, 2022-09
    94/100 stars
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    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.

    Journal: bioRxiv

    Article Title: Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection

    doi: 10.1101/2022.08.16.502937

    Figure Lengend Snippet: Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.

    Article Snippet: Protein was purified in the same buffer with lower ionic strength (70 mM NaCl instead), through an affinity Nickel-NTA Agarose Resin (Gold Biotechnology, Missouri, United States) column by gravity.

    Techniques: Expressing, SDS Page, Protein Purification, Marker, Purification, Inhibition, Activity Assay

    ELISA assay for detecting His-tagged proteins in TXTL. His Tag Elisa kit (LS-F55748) was purchased from LifeSpan Bioscience, inc. The cell-free preps were diluted to one-fifth with water, and the ELISA assay was performed following the manufacturer’s protocol. The standard curve was prepared using the standard sample prepared by the manufacturer. We confirmed the significant decrease of His-tagged protein in the Hina HI extract (After Ni-NTA treatment) compared to the Hina HI extract (Before Ni-NTA treatment. Error bars indicate SEM, n=3.

    Journal: bioRxiv

    Article Title: A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection

    doi: 10.1101/2022.07.28.501919

    Figure Lengend Snippet: ELISA assay for detecting His-tagged proteins in TXTL. His Tag Elisa kit (LS-F55748) was purchased from LifeSpan Bioscience, inc. The cell-free preps were diluted to one-fifth with water, and the ELISA assay was performed following the manufacturer’s protocol. The standard curve was prepared using the standard sample prepared by the manufacturer. We confirmed the significant decrease of His-tagged protein in the Hina HI extract (After Ni-NTA treatment) compared to the Hina HI extract (Before Ni-NTA treatment. Error bars indicate SEM, n=3.

    Article Snippet: 100 μl of Ni-NTA agarose beads was washed twice with 500 μl of water, then washed twice with wash buffer A (10 mM Tris-acetate pH 8.2, 14 mM magnesium acetate, 60 mM potassium acetate, 2 mM DTT).

    Techniques: Enzyme-linked Immunosorbent Assay