Journal: Frontiers in Microbiology
Article Title: Disruption of Protein Mannosylation Affects Candida guilliermondii Cell Wall, Immune Sensing, and Virulence
Figure Lengend Snippet: C. guilliermondii pmr1 Δ null mutant shows increased susceptibility to cell wall perturbing agents . The C. guilliermondii strains ATCC 6260, KU141 (Parental), HMY134 ( pmr1 Δ), and HMY138 ( pmr1 Δ + PMR1 ) were incubated with different concentrations of either Calcofluor White, Congo Red, Tunicamycin, or Hygromycin B, and growth was determined after incubation for 24 h at 30°C. Growth data were normalized as percentage of those generated with the same strain without treatment. For data normalization, growth results are shown as percentage of those obtained with the same strain growing in the absence of any perturbing agent. Data are means ± SD of three independent experiments performed in duplicates. For all the agents tested, the null mutant sensitivity was significantly different to that of the control strains ( P
Article Snippet: The maximum concentrations tested for each agent were: 400 μg/mL Calcofluor White (CFW, Sigma), 200 μg/mL Congo Red (Sigma), 300 μg/mL hygromycin B (GoldBio); 50 μg/mL tunicamycin (Sigma) and 0.1% (w/v) SDS (Invitrogen).
Techniques: Mutagenesis, Incubation, Generated