nimodipine  (Alomone Labs)


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    Structured Review

    Alomone Labs nimodipine
    <t>Nimodipine</t> alters GnRH induced calcium responses in gonadotropes of wild-type and Ctail mice. The analysis in Figure 8 - figure supplement 1 was repeated but with the L-type calcium channel blocker nimodipine (Nim) applied prior to and during the second GnRH pulse. Raster plots of GnRH induced calcium responses in the absence (left) and presence of nimodipine (right) in gonadotropes from a representative adult male (A) wild-type and (D) Ctail mouse. The two stimuli were separated by 60 min wash with Krebs-Ringer. Comparisons of AUC from a (B) wild-type (275 cells; 11.5 ± 4.7 vs. 7.1 ± 2.8 a.u. for GnRH and GnRH/Nim, respectively; p
    Nimodipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nimodipine/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nimodipine - by Bioz Stars, 2022-07
    93/100 stars

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    1) Product Images from "Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice"

    Article Title: Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice

    Journal: bioRxiv

    doi: 10.1101/2021.09.13.460073

    Nimodipine alters GnRH induced calcium responses in gonadotropes of wild-type and Ctail mice. The analysis in Figure 8 - figure supplement 1 was repeated but with the L-type calcium channel blocker nimodipine (Nim) applied prior to and during the second GnRH pulse. Raster plots of GnRH induced calcium responses in the absence (left) and presence of nimodipine (right) in gonadotropes from a representative adult male (A) wild-type and (D) Ctail mouse. The two stimuli were separated by 60 min wash with Krebs-Ringer. Comparisons of AUC from a (B) wild-type (275 cells; 11.5 ± 4.7 vs. 7.1 ± 2.8 a.u. for GnRH and GnRH/Nim, respectively; p
    Figure Legend Snippet: Nimodipine alters GnRH induced calcium responses in gonadotropes of wild-type and Ctail mice. The analysis in Figure 8 - figure supplement 1 was repeated but with the L-type calcium channel blocker nimodipine (Nim) applied prior to and during the second GnRH pulse. Raster plots of GnRH induced calcium responses in the absence (left) and presence of nimodipine (right) in gonadotropes from a representative adult male (A) wild-type and (D) Ctail mouse. The two stimuli were separated by 60 min wash with Krebs-Ringer. Comparisons of AUC from a (B) wild-type (275 cells; 11.5 ± 4.7 vs. 7.1 ± 2.8 a.u. for GnRH and GnRH/Nim, respectively; p

    Techniques Used: Mouse Assay

    GnRH induced ERK1/2 phosphorylation is Gα q/11 - and PKC-dependent, and calcium-independent in homologous LβT2 cells. (A) LβT2 cells were pretreated with 10 µM FR900359 (Gα q/11 inhibitor) or vehicle (DMSO) for 1 h, and then treated with vehicle (water) or 10 nM GnRH for 5- or 15-min. Whole cell protein lysates were collected and subjected to SDS-PAGE and western blotting with phospho- (top) or total (bottom) ERK1/2 antibodies. Blots from one of two replicate experiments are shown. (B) Data from independent duplicate experiments exemplified in panel A were quantified by normalizing the densitometry for the pERK1/2 bands to the total ERK1/2 bands. Data are presented as fold phospho-ERK1/2 relative to the control condition. Bar height reflects the group mean. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Vehicle vs. FR900359: 0 min GnRH p > 0.9999; 5 min GnRH p = 0.0106; 15 min GnRH p = 0.0085]. (C) LβT2 cells were pretreated for 20 min with vehicle (DMSO) or 5 µM Gö6983 for 20 min followed by treatment with vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (D) Data from 3 independent experiments in exemplified panel C were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control (vehicle) vs. GnRH (vehicle) p = 0.0048; Control (Gö6983) vs. GnRH (Gö6983) p = 0.3470. Control (vehicle) vs. Control (Gö6983) p = 0.9472; GnRH (vehicle) vs. GnRH (Gö6983) p = 0.0389. (E) LβT2 cells were pretreated for 20 min with vehicle (DMSO), 20 µM BAPTA-AM, 5 µM or 10 µM nimodipine, or 10 µM U0126 for 20 min followed by vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (F) Data from 3 independent experiments in panel E were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control vs. GnRH: Vehicle p = 0.0103; BAPTA-AM p = 0.0229; Nimodipine (5 µM) p = 0.0377; Nimodipine (10 µM) p = 0.0350; U1026 p > 0.9999.
    Figure Legend Snippet: GnRH induced ERK1/2 phosphorylation is Gα q/11 - and PKC-dependent, and calcium-independent in homologous LβT2 cells. (A) LβT2 cells were pretreated with 10 µM FR900359 (Gα q/11 inhibitor) or vehicle (DMSO) for 1 h, and then treated with vehicle (water) or 10 nM GnRH for 5- or 15-min. Whole cell protein lysates were collected and subjected to SDS-PAGE and western blotting with phospho- (top) or total (bottom) ERK1/2 antibodies. Blots from one of two replicate experiments are shown. (B) Data from independent duplicate experiments exemplified in panel A were quantified by normalizing the densitometry for the pERK1/2 bands to the total ERK1/2 bands. Data are presented as fold phospho-ERK1/2 relative to the control condition. Bar height reflects the group mean. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Vehicle vs. FR900359: 0 min GnRH p > 0.9999; 5 min GnRH p = 0.0106; 15 min GnRH p = 0.0085]. (C) LβT2 cells were pretreated for 20 min with vehicle (DMSO) or 5 µM Gö6983 for 20 min followed by treatment with vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (D) Data from 3 independent experiments in exemplified panel C were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control (vehicle) vs. GnRH (vehicle) p = 0.0048; Control (Gö6983) vs. GnRH (Gö6983) p = 0.3470. Control (vehicle) vs. Control (Gö6983) p = 0.9472; GnRH (vehicle) vs. GnRH (Gö6983) p = 0.0389. (E) LβT2 cells were pretreated for 20 min with vehicle (DMSO), 20 µM BAPTA-AM, 5 µM or 10 µM nimodipine, or 10 µM U0126 for 20 min followed by vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (F) Data from 3 independent experiments in panel E were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control vs. GnRH: Vehicle p = 0.0103; BAPTA-AM p = 0.0229; Nimodipine (5 µM) p = 0.0377; Nimodipine (10 µM) p = 0.0350; U1026 p > 0.9999.

    Techniques Used: SDS Page, Western Blot

    GnRH-induced Fshb and Lhb expression does not depend on calcium entry via L-type channels in homologous LβT2 cells. (A) Relative Fshb and (B) Lhb expression in LβT2 cells treated with vehicle (DMSO) or 10 µM nimodipine for 20 min followed by treatment with water (vehicle) or high frequency GnRH (10 nM) pulses. Gene expression was assessed by RT-qPCR and normalized to Rpl19 . Data reflect the means of 2 independent experiments. Data were analyzed with two-way ANOVAs, followed by post-hoc Tukey’s test for multiple comparisons. Bars with different letters differed significantly. Panel A: Control (vehicle) vs. GnRH (vehicle) p = 0.0178; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0232; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8545; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0235. Panel B: Control (vehicle) vs. GnRH (vehicle) p = 0.0061; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0043; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8770; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0178.
    Figure Legend Snippet: GnRH-induced Fshb and Lhb expression does not depend on calcium entry via L-type channels in homologous LβT2 cells. (A) Relative Fshb and (B) Lhb expression in LβT2 cells treated with vehicle (DMSO) or 10 µM nimodipine for 20 min followed by treatment with water (vehicle) or high frequency GnRH (10 nM) pulses. Gene expression was assessed by RT-qPCR and normalized to Rpl19 . Data reflect the means of 2 independent experiments. Data were analyzed with two-way ANOVAs, followed by post-hoc Tukey’s test for multiple comparisons. Bars with different letters differed significantly. Panel A: Control (vehicle) vs. GnRH (vehicle) p = 0.0178; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0232; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8545; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0235. Panel B: Control (vehicle) vs. GnRH (vehicle) p = 0.0061; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0043; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8770; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0178.

    Techniques Used: Expressing, Quantitative RT-PCR

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    Alomone Labs nimodipine
    <t>Nimodipine</t> alters GnRH induced calcium responses in gonadotropes of wild-type and Ctail mice. The analysis in Figure 8 - figure supplement 1 was repeated but with the L-type calcium channel blocker nimodipine (Nim) applied prior to and during the second GnRH pulse. Raster plots of GnRH induced calcium responses in the absence (left) and presence of nimodipine (right) in gonadotropes from a representative adult male (A) wild-type and (D) Ctail mouse. The two stimuli were separated by 60 min wash with Krebs-Ringer. Comparisons of AUC from a (B) wild-type (275 cells; 11.5 ± 4.7 vs. 7.1 ± 2.8 a.u. for GnRH and GnRH/Nim, respectively; p
    Nimodipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nimodipine/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nimodipine - by Bioz Stars, 2022-07
    93/100 stars
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    Nimodipine alters GnRH induced calcium responses in gonadotropes of wild-type and Ctail mice. The analysis in Figure 8 - figure supplement 1 was repeated but with the L-type calcium channel blocker nimodipine (Nim) applied prior to and during the second GnRH pulse. Raster plots of GnRH induced calcium responses in the absence (left) and presence of nimodipine (right) in gonadotropes from a representative adult male (A) wild-type and (D) Ctail mouse. The two stimuli were separated by 60 min wash with Krebs-Ringer. Comparisons of AUC from a (B) wild-type (275 cells; 11.5 ± 4.7 vs. 7.1 ± 2.8 a.u. for GnRH and GnRH/Nim, respectively; p

    Journal: bioRxiv

    Article Title: Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice

    doi: 10.1101/2021.09.13.460073

    Figure Lengend Snippet: Nimodipine alters GnRH induced calcium responses in gonadotropes of wild-type and Ctail mice. The analysis in Figure 8 - figure supplement 1 was repeated but with the L-type calcium channel blocker nimodipine (Nim) applied prior to and during the second GnRH pulse. Raster plots of GnRH induced calcium responses in the absence (left) and presence of nimodipine (right) in gonadotropes from a representative adult male (A) wild-type and (D) Ctail mouse. The two stimuli were separated by 60 min wash with Krebs-Ringer. Comparisons of AUC from a (B) wild-type (275 cells; 11.5 ± 4.7 vs. 7.1 ± 2.8 a.u. for GnRH and GnRH/Nim, respectively; p

    Article Snippet: To evaluate the contribution of voltage-gated calcium channels, after 1 h of recovery, tissue was incubated for 30 s with 20 μM nimodipine (ALOMONE LABS N-150 N150SM0250; Jerusalem, Israel) followed by a second application of 10 nM GnRH alone ( ) or in combination with 20 μM nimodipine ( ) for 30 s. Finally, to determine cell viability, high potassium solution (50 KCl mM, 120 NaCl mM, 10 HEPES mM, 2 CaCl2 mM, pH 7.4) was applied for 30 s. For each condition, the numbers of animals and cells analyzed are indicated in the figure legends.

    Techniques: Mouse Assay

    GnRH induced ERK1/2 phosphorylation is Gα q/11 - and PKC-dependent, and calcium-independent in homologous LβT2 cells. (A) LβT2 cells were pretreated with 10 µM FR900359 (Gα q/11 inhibitor) or vehicle (DMSO) for 1 h, and then treated with vehicle (water) or 10 nM GnRH for 5- or 15-min. Whole cell protein lysates were collected and subjected to SDS-PAGE and western blotting with phospho- (top) or total (bottom) ERK1/2 antibodies. Blots from one of two replicate experiments are shown. (B) Data from independent duplicate experiments exemplified in panel A were quantified by normalizing the densitometry for the pERK1/2 bands to the total ERK1/2 bands. Data are presented as fold phospho-ERK1/2 relative to the control condition. Bar height reflects the group mean. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Vehicle vs. FR900359: 0 min GnRH p > 0.9999; 5 min GnRH p = 0.0106; 15 min GnRH p = 0.0085]. (C) LβT2 cells were pretreated for 20 min with vehicle (DMSO) or 5 µM Gö6983 for 20 min followed by treatment with vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (D) Data from 3 independent experiments in exemplified panel C were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control (vehicle) vs. GnRH (vehicle) p = 0.0048; Control (Gö6983) vs. GnRH (Gö6983) p = 0.3470. Control (vehicle) vs. Control (Gö6983) p = 0.9472; GnRH (vehicle) vs. GnRH (Gö6983) p = 0.0389. (E) LβT2 cells were pretreated for 20 min with vehicle (DMSO), 20 µM BAPTA-AM, 5 µM or 10 µM nimodipine, or 10 µM U0126 for 20 min followed by vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (F) Data from 3 independent experiments in panel E were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control vs. GnRH: Vehicle p = 0.0103; BAPTA-AM p = 0.0229; Nimodipine (5 µM) p = 0.0377; Nimodipine (10 µM) p = 0.0350; U1026 p > 0.9999.

    Journal: bioRxiv

    Article Title: Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice

    doi: 10.1101/2021.09.13.460073

    Figure Lengend Snippet: GnRH induced ERK1/2 phosphorylation is Gα q/11 - and PKC-dependent, and calcium-independent in homologous LβT2 cells. (A) LβT2 cells were pretreated with 10 µM FR900359 (Gα q/11 inhibitor) or vehicle (DMSO) for 1 h, and then treated with vehicle (water) or 10 nM GnRH for 5- or 15-min. Whole cell protein lysates were collected and subjected to SDS-PAGE and western blotting with phospho- (top) or total (bottom) ERK1/2 antibodies. Blots from one of two replicate experiments are shown. (B) Data from independent duplicate experiments exemplified in panel A were quantified by normalizing the densitometry for the pERK1/2 bands to the total ERK1/2 bands. Data are presented as fold phospho-ERK1/2 relative to the control condition. Bar height reflects the group mean. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Vehicle vs. FR900359: 0 min GnRH p > 0.9999; 5 min GnRH p = 0.0106; 15 min GnRH p = 0.0085]. (C) LβT2 cells were pretreated for 20 min with vehicle (DMSO) or 5 µM Gö6983 for 20 min followed by treatment with vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (D) Data from 3 independent experiments in exemplified panel C were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control (vehicle) vs. GnRH (vehicle) p = 0.0048; Control (Gö6983) vs. GnRH (Gö6983) p = 0.3470. Control (vehicle) vs. Control (Gö6983) p = 0.9472; GnRH (vehicle) vs. GnRH (Gö6983) p = 0.0389. (E) LβT2 cells were pretreated for 20 min with vehicle (DMSO), 20 µM BAPTA-AM, 5 µM or 10 µM nimodipine, or 10 µM U0126 for 20 min followed by vehicle (water) or 10 nM GnRH for 5 min. Western blots were performed as in panel A. (F) Data from 3 independent experiments in panel E were presented and quantified as in panel B. Data were analyzed by two-way ANOVA, followed by Sidak’s post-hoc comparison tests. Bars with different letters differed significantly. Control vs. GnRH: Vehicle p = 0.0103; BAPTA-AM p = 0.0229; Nimodipine (5 µM) p = 0.0377; Nimodipine (10 µM) p = 0.0350; U1026 p > 0.9999.

    Article Snippet: To evaluate the contribution of voltage-gated calcium channels, after 1 h of recovery, tissue was incubated for 30 s with 20 μM nimodipine (ALOMONE LABS N-150 N150SM0250; Jerusalem, Israel) followed by a second application of 10 nM GnRH alone ( ) or in combination with 20 μM nimodipine ( ) for 30 s. Finally, to determine cell viability, high potassium solution (50 KCl mM, 120 NaCl mM, 10 HEPES mM, 2 CaCl2 mM, pH 7.4) was applied for 30 s. For each condition, the numbers of animals and cells analyzed are indicated in the figure legends.

    Techniques: SDS Page, Western Blot

    GnRH-induced Fshb and Lhb expression does not depend on calcium entry via L-type channels in homologous LβT2 cells. (A) Relative Fshb and (B) Lhb expression in LβT2 cells treated with vehicle (DMSO) or 10 µM nimodipine for 20 min followed by treatment with water (vehicle) or high frequency GnRH (10 nM) pulses. Gene expression was assessed by RT-qPCR and normalized to Rpl19 . Data reflect the means of 2 independent experiments. Data were analyzed with two-way ANOVAs, followed by post-hoc Tukey’s test for multiple comparisons. Bars with different letters differed significantly. Panel A: Control (vehicle) vs. GnRH (vehicle) p = 0.0178; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0232; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8545; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0235. Panel B: Control (vehicle) vs. GnRH (vehicle) p = 0.0061; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0043; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8770; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0178.

    Journal: bioRxiv

    Article Title: Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice

    doi: 10.1101/2021.09.13.460073

    Figure Lengend Snippet: GnRH-induced Fshb and Lhb expression does not depend on calcium entry via L-type channels in homologous LβT2 cells. (A) Relative Fshb and (B) Lhb expression in LβT2 cells treated with vehicle (DMSO) or 10 µM nimodipine for 20 min followed by treatment with water (vehicle) or high frequency GnRH (10 nM) pulses. Gene expression was assessed by RT-qPCR and normalized to Rpl19 . Data reflect the means of 2 independent experiments. Data were analyzed with two-way ANOVAs, followed by post-hoc Tukey’s test for multiple comparisons. Bars with different letters differed significantly. Panel A: Control (vehicle) vs. GnRH (vehicle) p = 0.0178; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0232; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8545; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0235. Panel B: Control (vehicle) vs. GnRH (vehicle) p = 0.0061; Control (vehicle) vs. GnRH (Nimodipine) p = 0.0043; GnRH (vehicle) vs. GnRH (Nimodipine) p = 0.8770; Control (Nimodipine) vs. GnRH (Nimodipine) p = 0.0178.

    Article Snippet: To evaluate the contribution of voltage-gated calcium channels, after 1 h of recovery, tissue was incubated for 30 s with 20 μM nimodipine (ALOMONE LABS N-150 N150SM0250; Jerusalem, Israel) followed by a second application of 10 nM GnRH alone ( ) or in combination with 20 μM nimodipine ( ) for 30 s. Finally, to determine cell viability, high potassium solution (50 KCl mM, 120 NaCl mM, 10 HEPES mM, 2 CaCl2 mM, pH 7.4) was applied for 30 s. For each condition, the numbers of animals and cells analyzed are indicated in the figure legends.

    Techniques: Expressing, Quantitative RT-PCR