cryo tem  (Thermo Fisher)


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    Name:
    Glacios Cryo TEM
    Description:
    The new Thermo Scientific Glacios Cryo Transmission Electron Microscope Cryo TEM delivers a complete and affordable Cryo EM solution to a broad range of scientists It features 200 kV XFEG optics the industry leading Autoloader cryogenic sample manipulation robot and the same innovative automation for ease of use as on the Krios G4 Cryo TEM The Glacios Cryo TEM bundles all this into a small footprint that simplifies installation The designed in connectivity ensures a robust and contamination free pathway throughout the entire workflow from sample preparation and optimization to image acquisition and data processing The Glacios Cryo TEM can be used in a single particle analysis SPA workflow for pre optimization of sample quality as well as for high resolution SPA data acquisition Furthermore the Glacios Cryo TEM can also be configured with a Direct Electron Detector and or Volta Phase Plate to be a complete standalone SPA data acquisition solution
    Catalog Number:
    GLACIOSTEM
    Price:
    None
    Category:
    Transmssion Electron Microscope TEM
    Applications:
    Cryo-tomography, single particle analysis, MicroED
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    Structured Review

    Thermo Fisher cryo tem
    The new Thermo Scientific Glacios Cryo Transmission Electron Microscope Cryo TEM delivers a complete and affordable Cryo EM solution to a broad range of scientists It features 200 kV XFEG optics the industry leading Autoloader cryogenic sample manipulation robot and the same innovative automation for ease of use as on the Krios G4 Cryo TEM The Glacios Cryo TEM bundles all this into a small footprint that simplifies installation The designed in connectivity ensures a robust and contamination free pathway throughout the entire workflow from sample preparation and optimization to image acquisition and data processing The Glacios Cryo TEM can be used in a single particle analysis SPA workflow for pre optimization of sample quality as well as for high resolution SPA data acquisition Furthermore the Glacios Cryo TEM can also be configured with a Direct Electron Detector and or Volta Phase Plate to be a complete standalone SPA data acquisition solution
    https://www.bioz.com/result/cryo tem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem - by Bioz Stars, 2021-10
    99/100 stars

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    Related Articles

    Tomography:

    Article Title: Structural Basis and Mode of Action for Two Broadly Neutralizing Antibodies Against SARS-CoV-2 Emerging Variants of Concern
    Article Snippet: .. Cryo-electron Tomography Data Collection Cryo-grids were imaged on a cryo-transmission electron microscope (Titan Krios, Thermo Fisher Scientific) operated at 300 kV, using a Gatan K3 direct electron detector in counting mode with a 20 eV energy slit. ..

    Microscopy:

    Article Title: Structural Basis and Mode of Action for Two Broadly Neutralizing Antibodies Against SARS-CoV-2 Emerging Variants of Concern
    Article Snippet: .. Cryo-electron Tomography Data Collection Cryo-grids were imaged on a cryo-transmission electron microscope (Titan Krios, Thermo Fisher Scientific) operated at 300 kV, using a Gatan K3 direct electron detector in counting mode with a 20 eV energy slit. ..

    Article Title: Stepwise maturation of the peptidyl transferase region of human mitoribosomes
    Article Snippet: .. Both datasets were collected on a Titan Krios cryo-transmission electron microscope (Thermo Fisher Scientific) operating at 300 kV. ..

    Article Title: Small Molecule Microcrystal Electron Diffraction for the Pharmaceutical Industry–Lessons Learned From Examining Over Fifty Samples
    Article Snippet: .. Electron microscopy was performed using a Thermo Fisher Scientific (Hillsboro, OR, United States) Glacios Cryo Transmission Electron Microscope operated at 200 kV with the cryostage at −192°C. ..

    Article Title: BacPROTACs mediate targeted protein degradation in bacteria
    Article Snippet: .. The quality of the grids was screened using a Thermo Scientific Glacios Cryo Transmission Electron Microscope equipped with a Falcon3 Direct Electron Detector. ..

    Article Title: Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants
    Article Snippet: .. Transmission electron microscopy (TEM) and 2D class averaging Electron microscopy was performed by NanoImaging Services (San Diego, CA) with a Thermo Fisher Scientific Glacios Cryo Transmission Electron Microscope, operated at 200kV equipped with a Falcon 3 direct electron detector. ..

    Article Title: Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription
    Article Snippet: .. Automated data collections of (i) the replication early-elongation state, (ii) the replication late-elongation state, (iii) the transcription initiation state and (iv) the transcription early-elongation state were performed on a 200 kV Glacios cryo-TEM microscope (Thermo Fischer Scientific) equipped with a K2 direct electron detector (Gatan) using SerialEM . ..

    Electron Microscopy:

    Article Title: Small Molecule Microcrystal Electron Diffraction for the Pharmaceutical Industry–Lessons Learned From Examining Over Fifty Samples
    Article Snippet: .. Electron microscopy was performed using a Thermo Fisher Scientific (Hillsboro, OR, United States) Glacios Cryo Transmission Electron Microscope operated at 200 kV with the cryostage at −192°C. ..

    Article Title: Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants
    Article Snippet: .. Transmission electron microscopy (TEM) and 2D class averaging Electron microscopy was performed by NanoImaging Services (San Diego, CA) with a Thermo Fisher Scientific Glacios Cryo Transmission Electron Microscope, operated at 200kV equipped with a Falcon 3 direct electron detector. ..

    Transmission Assay:

    Article Title: Small Molecule Microcrystal Electron Diffraction for the Pharmaceutical Industry–Lessons Learned From Examining Over Fifty Samples
    Article Snippet: .. Electron microscopy was performed using a Thermo Fisher Scientific (Hillsboro, OR, United States) Glacios Cryo Transmission Electron Microscope operated at 200 kV with the cryostage at −192°C. ..

    Article Title: BacPROTACs mediate targeted protein degradation in bacteria
    Article Snippet: .. The quality of the grids was screened using a Thermo Scientific Glacios Cryo Transmission Electron Microscope equipped with a Falcon3 Direct Electron Detector. ..

    Article Title: Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants
    Article Snippet: .. Transmission electron microscopy (TEM) and 2D class averaging Electron microscopy was performed by NanoImaging Services (San Diego, CA) with a Thermo Fisher Scientific Glacios Cryo Transmission Electron Microscope, operated at 200kV equipped with a Falcon 3 direct electron detector. ..

    Transmission Electron Microscopy:

    Article Title: Immunogenicity and In vivo protection of a variant nanoparticle vaccine that confers broad protection against emerging SARS-CoV-2 variants
    Article Snippet: .. Transmission electron microscopy (TEM) and 2D class averaging Electron microscopy was performed by NanoImaging Services (San Diego, CA) with a Thermo Fisher Scientific Glacios Cryo Transmission Electron Microscope, operated at 200kV equipped with a Falcon 3 direct electron detector. ..

    Article Title: Large-area synthesis of nanoscopic catalyst-decorated conductive MOF film using microfluidic-based solution shearing
    Article Snippet: .. Cryo Field Emission TEM (Glacios, Thermo Fisher) at 200 kV, field-emission TEM (Tecnai G2 S-Twin, FEI) at 300 kV, and spherical aberration-corrected TEM (JEMARM200F, JEOL) at 200 kV were conducted to investigate the microstructure of the samples. ..

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    Thermo Fisher thermo scientific glacios cryo tem
    Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and <t>Cryo-TEM</t> image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P
    Thermo Scientific Glacios Cryo Tem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo scientific glacios cryo tem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermo scientific glacios cryo tem - by Bioz Stars, 2021-10
    99/100 stars
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    Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and Cryo-TEM image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P

    Journal: Nature nanotechnology

    Article Title: Vitamin Lipid Nanoparticles Enable Adoptive Macrophage Transfer for the Treatment of Multidrug-Resistant Bacterial Sepsis

    doi: 10.1038/s41565-019-0600-1

    Figure Lengend Snippet: Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and Cryo-TEM image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P

    Article Snippet: The morphology of VC LNPs was examined on Thermo Scientific™ Glacios™ Cryo-TEM using the methods described before .

    Techniques: Expressing, Transmission Electron Microscopy, Incubation, Two Tailed Test

    Electron tomography of vitreously frozen Pichia pastoris cells prepared by cryo-FIB. Multiple yeast cells can be found in a FIB prepared lamellae, as shown in (a) . An alcohol-oxidase crystal lattice inside a peroxisome is hardly visible in an un-tilted projection image (b) . However in (c) , the lattice is clearly seen in a highly tilted 7.2-nm-thick tomographic slice, attesting to good markerless alignment of the tilt-series images. In a z-slice from another cryo-tomogram (d) , large protein complexes are seen. In the white circle, magnified in (e) , cytoplasmic ribosomes (white arrows) are seen. Some mitochondrial complexes, which are likely rows of ATP synthase, are also clearly resolved (black arrows). Within the black circle in (d) , shown magnified in (f) , is a crystalline array of alcohol oxidase.

    Journal: Journal of structural biology

    Article Title: Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM

    doi: 10.1016/j.jsb.2017.05.011

    Figure Lengend Snippet: Electron tomography of vitreously frozen Pichia pastoris cells prepared by cryo-FIB. Multiple yeast cells can be found in a FIB prepared lamellae, as shown in (a) . An alcohol-oxidase crystal lattice inside a peroxisome is hardly visible in an un-tilted projection image (b) . However in (c) , the lattice is clearly seen in a highly tilted 7.2-nm-thick tomographic slice, attesting to good markerless alignment of the tilt-series images. In a z-slice from another cryo-tomogram (d) , large protein complexes are seen. In the white circle, magnified in (e) , cytoplasmic ribosomes (white arrows) are seen. Some mitochondrial complexes, which are likely rows of ATP synthase, are also clearly resolved (black arrows). Within the black circle in (d) , shown magnified in (f) , is a crystalline array of alcohol oxidase.

    Article Snippet: Commercial cryosystems are available for cryo-FIB specimen preparation, however re-engineering and additional fixtures are often essential for reliable results with a particular cryo-FIB and cryo-transmission electron microscope (cryo-TEM).

    Techniques: Tomography

    Cryo-FIB specimen preparation. (a) The modified cryotransfer holder, holding a modified cartridge, mounted on the FIB-SEM coldstage, showing the relative orientations of the electron and ion beams. (b) An “electron-beam view” of two FIB lamellae (in dotted squares). (c) An “ion-beam view” (end-on) of a FIB lamella. (d) An electron-beam view at higher magnification of a FIB lamella, showing faint outlines of the yeast cells. (e) The TEM cryotransfer loading station. Under liquid nitrogen, the shutter of the modified FIB-SEM cryotransfer holder is opened and the cartridge is inserted into the TEM cryotransfer block, shown in (f). (f) A TEM cartridge inserted in the TEM cryo-transfer block.

    Journal: Journal of structural biology

    Article Title: Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM

    doi: 10.1016/j.jsb.2017.05.011

    Figure Lengend Snippet: Cryo-FIB specimen preparation. (a) The modified cryotransfer holder, holding a modified cartridge, mounted on the FIB-SEM coldstage, showing the relative orientations of the electron and ion beams. (b) An “electron-beam view” of two FIB lamellae (in dotted squares). (c) An “ion-beam view” (end-on) of a FIB lamella. (d) An electron-beam view at higher magnification of a FIB lamella, showing faint outlines of the yeast cells. (e) The TEM cryotransfer loading station. Under liquid nitrogen, the shutter of the modified FIB-SEM cryotransfer holder is opened and the cartridge is inserted into the TEM cryotransfer block, shown in (f). (f) A TEM cartridge inserted in the TEM cryo-transfer block.

    Article Snippet: Commercial cryosystems are available for cryo-FIB specimen preparation, however re-engineering and additional fixtures are often essential for reliable results with a particular cryo-FIB and cryo-transmission electron microscope (cryo-TEM).

    Techniques: Modification, Transmission Electron Microscopy, Blocking Assay

    Characterization of Pt@C-MOF thin films fabricated by MiCS. a Cryo-TEM image of Pt@Cu 3 (HHTP) 2 under low magnification. b High-resolution Cryo-TEM image of Pt@Cu 3 (HHTP) 2 without Pt particles in the MOF pores. c High-resolution Cryo-TEM image of Pt particles immobilized in the MOF pores. d Integrated intensity of Pt@Cu 3 (HHTP) 2 plotted over six unit cells along the [100] direction, indicating a pattern when Pt particle is introduced into the pores. e Thickness control of Pt@Cu 3 (HHTP) 2 thin film via controlling the shearing speeds. f UV–Vis spectrum of different thicknesses of Pt@Cu 3 (HHTP) 2 thin film. g PXRD data of Cu 3 (HHTP) 2 and Pt@Cu 3 (HHTP) 2 . @ means –embedded.

    Journal: Nature Communications

    Article Title: Large-area synthesis of nanoscopic catalyst-decorated conductive MOF film using microfluidic-based solution shearing

    doi: 10.1038/s41467-021-24571-1

    Figure Lengend Snippet: Characterization of Pt@C-MOF thin films fabricated by MiCS. a Cryo-TEM image of Pt@Cu 3 (HHTP) 2 under low magnification. b High-resolution Cryo-TEM image of Pt@Cu 3 (HHTP) 2 without Pt particles in the MOF pores. c High-resolution Cryo-TEM image of Pt particles immobilized in the MOF pores. d Integrated intensity of Pt@Cu 3 (HHTP) 2 plotted over six unit cells along the [100] direction, indicating a pattern when Pt particle is introduced into the pores. e Thickness control of Pt@Cu 3 (HHTP) 2 thin film via controlling the shearing speeds. f UV–Vis spectrum of different thicknesses of Pt@Cu 3 (HHTP) 2 thin film. g PXRD data of Cu 3 (HHTP) 2 and Pt@Cu 3 (HHTP) 2 . @ means –embedded.

    Article Snippet: Cryo Field Emission TEM (Glacios, Thermo Fisher) at 200 kV, field-emission TEM (Tecnai G2 S-Twin, FEI) at 300 kV, and spherical aberration-corrected TEM (JEMARM200F, JEOL) at 200 kV were conducted to investigate the microstructure of the samples.

    Techniques: Transmission Electron Microscopy

    Characteristics of Ects present in the Ect -enriched and Ect -depleted plasma fractions. Representative wide-field and magnified cryo-TEM images of Ects isolated from a control subject (A-B) and a patient with diabetes (glucose level: 12.02 mM; HbA1C level: 9.2%) (E-F) . Representative plots of NTA size distribution of EVs in Ect -enriched and Ect -depleted plasma fractions from the control group (C-D) and diabetic patients (G-H) . Samples were analyzed in 1000 × dilutions.

    Journal: Theranostics

    Article Title: Circulating ectosomes: Determination of angiogenic microRNAs in type 2 diabetes

    doi: 10.7150/thno.23334

    Figure Lengend Snippet: Characteristics of Ects present in the Ect -enriched and Ect -depleted plasma fractions. Representative wide-field and magnified cryo-TEM images of Ects isolated from a control subject (A-B) and a patient with diabetes (glucose level: 12.02 mM; HbA1C level: 9.2%) (E-F) . Representative plots of NTA size distribution of EVs in Ect -enriched and Ect -depleted plasma fractions from the control group (C-D) and diabetic patients (G-H) . Samples were analyzed in 1000 × dilutions.

    Article Snippet: Cryogenic transmission electron microscopy Cryogenic transmission electron microscopy (Cryo-TEM) of the Ect -enriched plasma fraction was performed using a Tecnai F20 TWIN microscope (FEI Company, USA) equipped with a field emission gun and operated at an acceleration voltage of 200 kV.

    Techniques: Transmission Electron Microscopy, Isolation