glacios cryotem  (Thermo Fisher)


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    Thermo Fisher glacios cryotem
    Glacios Cryotem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glacios cryotem/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glacios cryotem - by Bioz Stars, 2022-01
    94/100 stars

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    Thermo Fisher thermo scientific glacios cryo tem
    Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and <t>Cryo-TEM</t> image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P
    Thermo Scientific Glacios Cryo Tem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo scientific glacios cryo tem/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermo scientific glacios cryo tem - by Bioz Stars, 2022-01
    94/100 stars
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    Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and Cryo-TEM image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P

    Journal: Nature nanotechnology

    Article Title: Vitamin Lipid Nanoparticles Enable Adoptive Macrophage Transfer for the Treatment of Multidrug-Resistant Bacterial Sepsis

    doi: 10.1038/s41565-019-0600-1

    Figure Lengend Snippet: Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and Cryo-TEM image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P

    Article Snippet: The morphology of VC LNPs was examined on Thermo Scientific™ Glacios™ Cryo-TEM using the methods described before .

    Techniques: Expressing, Transmission Electron Microscopy, Incubation, Two Tailed Test

    Characterization of Pt@C-MOF thin films fabricated by MiCS. a Cryo-TEM image of Pt@Cu 3 (HHTP) 2 under low magnification. b High-resolution Cryo-TEM image of Pt@Cu 3 (HHTP) 2 without Pt particles in the MOF pores. c High-resolution Cryo-TEM image of Pt particles immobilized in the MOF pores. d Integrated intensity of Pt@Cu 3 (HHTP) 2 plotted over six unit cells along the [100] direction, indicating a pattern when Pt particle is introduced into the pores. e Thickness control of Pt@Cu 3 (HHTP) 2 thin film via controlling the shearing speeds. f UV–Vis spectrum of different thicknesses of Pt@Cu 3 (HHTP) 2 thin film. g PXRD data of Cu 3 (HHTP) 2 and Pt@Cu 3 (HHTP) 2 . @ means –embedded.

    Journal: Nature Communications

    Article Title: Large-area synthesis of nanoscopic catalyst-decorated conductive MOF film using microfluidic-based solution shearing

    doi: 10.1038/s41467-021-24571-1

    Figure Lengend Snippet: Characterization of Pt@C-MOF thin films fabricated by MiCS. a Cryo-TEM image of Pt@Cu 3 (HHTP) 2 under low magnification. b High-resolution Cryo-TEM image of Pt@Cu 3 (HHTP) 2 without Pt particles in the MOF pores. c High-resolution Cryo-TEM image of Pt particles immobilized in the MOF pores. d Integrated intensity of Pt@Cu 3 (HHTP) 2 plotted over six unit cells along the [100] direction, indicating a pattern when Pt particle is introduced into the pores. e Thickness control of Pt@Cu 3 (HHTP) 2 thin film via controlling the shearing speeds. f UV–Vis spectrum of different thicknesses of Pt@Cu 3 (HHTP) 2 thin film. g PXRD data of Cu 3 (HHTP) 2 and Pt@Cu 3 (HHTP) 2 . @ means –embedded.

    Article Snippet: Cryo Field Emission TEM (Glacios, Thermo Fisher) at 200 kV, field-emission TEM (Tecnai G2 S-Twin, FEI) at 300 kV, and spherical aberration-corrected TEM (JEMARM200F, JEOL) at 200 kV were conducted to investigate the microstructure of the samples.

    Techniques: Transmission Electron Microscopy

    Biomimetic phospholipid- and glycolipid-derived nanoparticles for mRNA delivery. a Luminescence intensity of phospholipid- (PL) and glycolipid-derived (GL) nanoparticles delivering firefly luciferase (Fluc) mRNA to E.G7 cells. b Cryo-TEM image of PL1-mRNA nanoparticles (a representative image from one of five independent fields of view in a single experiment). Scale bar = 50 nm. c PL1 nanoparticles delivered GFP mRNA to E.G7 cells. d PL1-CD137 induced CD137 expression in E.G7 cells. e PL1-OX40 induced OX40 expression in EG.7 cells. f Scheme of GFP expression in B16F10 tumors after a single injection of free GFP mRNA or PL1-GFP. g GFP expression in CD4+, CD8+ T cells, after a single intratumoral injection with GFP mRNA ( n = 4) or PL1-GFP mRNA ( n = 5) in B16F10 tumors. Data in c , d and e are representative of three independent experiments. All data are presented as the mean ± S.E.M. Statistical significance in c , d , e was analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test, and data in g was analyzed using the two-tailed Student’s t -test. * P

    Journal: Nature Communications

    Article Title: Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy

    doi: 10.1038/s41467-021-27434-x

    Figure Lengend Snippet: Biomimetic phospholipid- and glycolipid-derived nanoparticles for mRNA delivery. a Luminescence intensity of phospholipid- (PL) and glycolipid-derived (GL) nanoparticles delivering firefly luciferase (Fluc) mRNA to E.G7 cells. b Cryo-TEM image of PL1-mRNA nanoparticles (a representative image from one of five independent fields of view in a single experiment). Scale bar = 50 nm. c PL1 nanoparticles delivered GFP mRNA to E.G7 cells. d PL1-CD137 induced CD137 expression in E.G7 cells. e PL1-OX40 induced OX40 expression in EG.7 cells. f Scheme of GFP expression in B16F10 tumors after a single injection of free GFP mRNA or PL1-GFP. g GFP expression in CD4+, CD8+ T cells, after a single intratumoral injection with GFP mRNA ( n = 4) or PL1-GFP mRNA ( n = 5) in B16F10 tumors. Data in c , d and e are representative of three independent experiments. All data are presented as the mean ± S.E.M. Statistical significance in c , d , e was analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test, and data in g was analyzed using the two-tailed Student’s t -test. * P

    Article Snippet: The morphology of nanoparticles was examined on a Thermo Scientific™ Glacios™ cryo-TEM.

    Techniques: Derivative Assay, Luciferase, Transmission Electron Microscopy, Expressing, Injection, Two Tailed Test

    Characteristics of Ects present in the Ect -enriched and Ect -depleted plasma fractions. Representative wide-field and magnified cryo-TEM images of Ects isolated from a control subject (A-B) and a patient with diabetes (glucose level: 12.02 mM; HbA1C level: 9.2%) (E-F) . Representative plots of NTA size distribution of EVs in Ect -enriched and Ect -depleted plasma fractions from the control group (C-D) and diabetic patients (G-H) . Samples were analyzed in 1000 × dilutions.

    Journal: Theranostics

    Article Title: Circulating ectosomes: Determination of angiogenic microRNAs in type 2 diabetes

    doi: 10.7150/thno.23334

    Figure Lengend Snippet: Characteristics of Ects present in the Ect -enriched and Ect -depleted plasma fractions. Representative wide-field and magnified cryo-TEM images of Ects isolated from a control subject (A-B) and a patient with diabetes (glucose level: 12.02 mM; HbA1C level: 9.2%) (E-F) . Representative plots of NTA size distribution of EVs in Ect -enriched and Ect -depleted plasma fractions from the control group (C-D) and diabetic patients (G-H) . Samples were analyzed in 1000 × dilutions.

    Article Snippet: Cryogenic transmission electron microscopy Cryogenic transmission electron microscopy (Cryo-TEM) of the Ect -enriched plasma fraction was performed using a Tecnai F20 TWIN microscope (FEI Company, USA) equipped with a field emission gun and operated at an acceleration voltage of 200 kV.

    Techniques: Transmission Electron Microscopy, Isolation