Journal: Nature nanotechnology
Article Title: Vitamin Lipid Nanoparticles Enable Adoptive Macrophage Transfer for the Treatment of Multidrug-Resistant Bacterial Sepsis
Figure Lengend Snippet: Screening, optimization, and characterization of vitamin lipid nanoparticles (VLNPs). a , mRNA delivery efficiency of VLNPs in RAW264.7 cells. b , Expression kinetics of mRNA delivered by V C LNPs in RAW264.7 cells. c , The first round of optimization: four levels and impact trend of each V C LNP’s component. d , Formulation table for the validation of the predicted formulation, and the second round of optimization: the mass ratio of V C -Lipid:mRNA. e , Fold changes of luminescence intensity in the two rounds of optimization. f , Characterization of the optimal V C LNP formulation, including size, polydispersity index (PDI), encapsulation efficiency, zeta potential, and Cryo-TEM image (Scale bar = 50 nm). g , Confocal imagines of the RAW264.7 cells incubated with V C LNPs encapsulating eGFP-CatB mRNA (Scale bar = 10 μm). Data in g are representative images from n = 3 independent experiments. h , Intracellular survival of MDR Staphylococcus aureus (MDRSA) in the RAW264.7 cells. Data in a , b , c , e , and h are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance was analyzed by the two-tailed Student’s t -test. * P
Article Snippet: The morphology of VC LNPs was examined on Thermo Scientific™ Glacios™ Cryo-TEM using the methods described before .
Techniques: Expressing, Transmission Electron Microscopy, Incubation, Two Tailed Test