par2 agonist  (Alomone Labs)

Journal: Cancer research

Article Title: Voltage-gated Na+ channel SCN5A is a key regulator of a gene transcriptional network that controls colon cancer invasion

doi: 10.1158/0008-5472.CAN-10-1169

Figure Lengend Snippet: Na v 1.5 contributes to invasive potential of colon cancer cells ( A ) The total number of invading SW620, SW480 and HT29 colon cancer cells was significantly reduced with 30 μM TTX compared to vehicle control. ( B ) siRNA-mediated knockdown of SCN5A

Article Snippet: Sections were incubated with anti-Nav1.5 polyclonal antibody (1:100) (Alomone labs) for three hours, followed by one hour with HRP labeled polymer conjugated anti-rabbit secondary antibody.

Techniques:

Journal: Cancer research

Article Title: Voltage-gated Na+ channel SCN5A is a key regulator of a gene transcriptional network that controls colon cancer invasion

doi: 10.1158/0008-5472.CAN-10-1169

Figure Lengend Snippet: SCN5A and predicted network genes involved in the invasive potential of HT29 cells. siRNA-mediated knockdown of individual genes proposed to be involved in the invasion network leads to a loss of invasion. ( A ) qRT-PCR was performed to validate mRNA knockdowns

Article Snippet: Sections were incubated with anti-Nav1.5 polyclonal antibody (1:100) (Alomone labs) for three hours, followed by one hour with HRP labeled polymer conjugated anti-rabbit secondary antibody.

Techniques: Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Telomerase increasing compound protects hippocampal neurons from amyloid beta toxicity by enhancing the expression of neurotrophins and plasticity related genes

doi: 10.1038/s41598-019-54741-7

Figure Lengend Snippet: AGS increases BDNF but not NGF gene expression in both Aβ treated and untreated cultures. Cultures were treated for 36 hr. with AGS-499 (200 nM) alone, Aβ (5 µM) alone, and Aβ (5 µM) in combination with AGS-499 (200 nM) or with its vehicle DMSO. AGS or DMSO treatments were renewed after 24 hr. Expression levels were quantified using RT-PCR and normalized to the untreated control (Control). ( a ) Relative BDNF gene expression in cultures (Mean ± SEM, n = 5 independent experiments), ANOVA test ***p

Article Snippet: The brain slices (5 μΜ) derived from vehicle (DMSO) and AGS-499 treated mice were subjected to immunofluorescence analysis using as first antibody: Guinea pig anti BDNF-antibody (1:100, # AGP-021, alomono labs, Israel) or anti-beta catenin antibody (1:100, β-Catenin (6B3) Rabbit mAb #9582, Cell Signaling technology, Danvers, MA, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Pain

Article Title: miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels

doi: 10.1097/j.pain.0000000000000971

Figure Lengend Snippet: Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

Article Snippet: Primary antibodies used for IF are Guinea pig anti-PGP9.5 (1:100 dilution, 14104, Neuromics, Edina, MN), Guinea pig anti-HCN1 (1:100, Alomone Labs, AGP203) rabbit anti-Cav2.3 antibody (1:80, Alomone Labs, ACC-006), Biotinylated-Isolectin B4 (1:100; B-1205, Vector, Burlingame, CA), Guinea pig Substance P (1:150; Neuromics GP14103), Anti-GFAP (1:500; NeuroMab clone N206A/8, UC Davis, Davis, CA) and Chicken anti-NF200 (1:500; Neuromics CH23015).

Techniques: Expressing, In Vivo, Mouse Assay