gdnf  (Alomone Labs)


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  • 94
    Name:
    G 240 human GDNF
    Description:
    Human Glial Derived Neurotrophic Factor Recombinant E coli
    Catalog Number:
    G-240
    Price:
    63.0
    Category:
    Protein
    Source:
    Recombinant, E. coli
    Applications:
    0
    Purity:
    >98% (HPLC)
    Size:
    5 mcg
    Format:
    Lyophilized from a 0.2 µm filtered solution.
    Molecular Weight:
    30.1 kDa.
    Molecule Name:
    GDNF, Glial-Derived Neurotrophic Factor
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    Structured Review

    Alomone Labs gdnf
    G 240 human GDNF
    Human Glial Derived Neurotrophic Factor Recombinant E coli
    https://www.bioz.com/result/gdnf/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdnf - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Targeting the Sigma-1 Receptor via Pridopidine Ameliorates Central Features of ALS Pathology in a SOD1G93A Model"

    Article Title: Targeting the Sigma-1 Receptor via Pridopidine Ameliorates Central Features of ALS Pathology in a SOD1G93A Model

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-019-1451-2

    Model. a Pridopidine triggers Neuroprotective pathways and promotes survival of SOD1 G93A MNs. b S1R targeting by pridopidine modulates axonal transport of BDNF, GDNF and mitochondria in SOD1 G93A MNs. c Pridopidine activates ERK signaling in MNs via S1R. d Pridopidine chaperones mSOD1 aggregates. e Pridopidine minimizes NMJ disruption and the subsequent muscle atrophy in SOD1 G93A models. Pridopidine also increases neurotrophic factor release from muscles
    Figure Legend Snippet: Model. a Pridopidine triggers Neuroprotective pathways and promotes survival of SOD1 G93A MNs. b S1R targeting by pridopidine modulates axonal transport of BDNF, GDNF and mitochondria in SOD1 G93A MNs. c Pridopidine activates ERK signaling in MNs via S1R. d Pridopidine chaperones mSOD1 aggregates. e Pridopidine minimizes NMJ disruption and the subsequent muscle atrophy in SOD1 G93A models. Pridopidine also increases neurotrophic factor release from muscles

    Techniques Used:

    Locally administered Pridopidine regulates the axonal transport of BDNF, GDNF, and mitochondria in co-culture of SOD1 G93A . a Schematic illustration of the experimental procedure. Primary skeletal myoblasts expressing BDNF-mCherry and GDNF-GFP are co-cultured with ventral spinal cord explants from WT or SOD1 G93A E12.5 mouse embryos. Pridopidine is exclusively applied at this phase to the distal compartment. At 7 days in co-culture, the axonal transport of muscle-derived BDNF-mCherry and GDNF-GFP, as well as the transport of mitochondria is imaged. b Representative confocal images of myocyte in the distal compartment expressing both BDNF-mCherry (red) and GDNF-GFP (green) and stained with MitoTracker (cyan). The white dashed line denotes an axon innervating a muscle containing BDNF-mCherry and GDNF-GFP particles as well as mitochondria. Scale bar: 20 µm. Lower panel includes magnified images of the axon. Yellow arrowheads denote the BDNF, GDNF, and mitochondria within the axon. Scale bar: 5 µm. c Representative time-lapse images show the retrograde axonal transport of muscle-derived BDNF-mCheery (red), GDNF-GFP (green), as well as the retrograde transport of mitochondria (cyan). White arrows denote co-transported BDNF-mCherry and GDNF-GFP. The arrowhead denotes co-transported BDNF-mCherry and mitochondria. Scale bar: 5 µm. Lower panel includes a kymograph of the entire time lapse movie demonstrating that most of the BDNF and GDNF, as well as mitochondria are retrogradely transported. Scale bars – horizontal: 5 µm vertical: 100 s. d–f Quantitative analysis of the instantaneous velocity of D) BDNF-mCherry, E) GDNF-GFP, and F) mitochondria from axons in co-culture of WT or SOD1 G93A reveals accelerated axonal transport of all of the above in SOD1 G93A compared to WT ( n = number of particle steps). Application of 0.1 µM pridopidine exclusively to the distal compartment is sufficient to restore the axonal transport properties of BDNF-mCherry and GDNF-GFP. However, the retrograde mitochondrial transport is further accelerated in the pridopidine-treated cultures. g–i Stacked distribution plots of the average velocity of G) BDNF-mCherry, H) GDNF-GFP, and I) mitochondria display higher transport velocities for all of the above in SOD1 G93A co-cultures. dashed lines highlight the changes in transporting populations of GDNF-GFP, BDNF-mCherry and mitochondria. Whereas 0.1 µM pridopidine moderates the velocities of BDNF-mCherry and GDNF-GFP, a specific population of mitochondria is transported even faster in response to the pridopidine treatment. Data are shown as the mean ± SEM. *** p value
    Figure Legend Snippet: Locally administered Pridopidine regulates the axonal transport of BDNF, GDNF, and mitochondria in co-culture of SOD1 G93A . a Schematic illustration of the experimental procedure. Primary skeletal myoblasts expressing BDNF-mCherry and GDNF-GFP are co-cultured with ventral spinal cord explants from WT or SOD1 G93A E12.5 mouse embryos. Pridopidine is exclusively applied at this phase to the distal compartment. At 7 days in co-culture, the axonal transport of muscle-derived BDNF-mCherry and GDNF-GFP, as well as the transport of mitochondria is imaged. b Representative confocal images of myocyte in the distal compartment expressing both BDNF-mCherry (red) and GDNF-GFP (green) and stained with MitoTracker (cyan). The white dashed line denotes an axon innervating a muscle containing BDNF-mCherry and GDNF-GFP particles as well as mitochondria. Scale bar: 20 µm. Lower panel includes magnified images of the axon. Yellow arrowheads denote the BDNF, GDNF, and mitochondria within the axon. Scale bar: 5 µm. c Representative time-lapse images show the retrograde axonal transport of muscle-derived BDNF-mCheery (red), GDNF-GFP (green), as well as the retrograde transport of mitochondria (cyan). White arrows denote co-transported BDNF-mCherry and GDNF-GFP. The arrowhead denotes co-transported BDNF-mCherry and mitochondria. Scale bar: 5 µm. Lower panel includes a kymograph of the entire time lapse movie demonstrating that most of the BDNF and GDNF, as well as mitochondria are retrogradely transported. Scale bars – horizontal: 5 µm vertical: 100 s. d–f Quantitative analysis of the instantaneous velocity of D) BDNF-mCherry, E) GDNF-GFP, and F) mitochondria from axons in co-culture of WT or SOD1 G93A reveals accelerated axonal transport of all of the above in SOD1 G93A compared to WT ( n = number of particle steps). Application of 0.1 µM pridopidine exclusively to the distal compartment is sufficient to restore the axonal transport properties of BDNF-mCherry and GDNF-GFP. However, the retrograde mitochondrial transport is further accelerated in the pridopidine-treated cultures. g–i Stacked distribution plots of the average velocity of G) BDNF-mCherry, H) GDNF-GFP, and I) mitochondria display higher transport velocities for all of the above in SOD1 G93A co-cultures. dashed lines highlight the changes in transporting populations of GDNF-GFP, BDNF-mCherry and mitochondria. Whereas 0.1 µM pridopidine moderates the velocities of BDNF-mCherry and GDNF-GFP, a specific population of mitochondria is transported even faster in response to the pridopidine treatment. Data are shown as the mean ± SEM. *** p value

    Techniques Used: Co-Culture Assay, Expressing, Cell Culture, Derivative Assay, Staining

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    Alomone Labs gdnf
    Model. a Pridopidine triggers Neuroprotective pathways and promotes survival of SOD1 G93A MNs. b S1R targeting by pridopidine modulates axonal transport of <t>BDNF,</t> <t>GDNF</t> and mitochondria in SOD1 G93A MNs. c Pridopidine activates ERK signaling in MNs via S1R. d Pridopidine chaperones mSOD1 aggregates. e Pridopidine minimizes NMJ disruption and the subsequent muscle atrophy in SOD1 G93A models. Pridopidine also increases neurotrophic factor release from muscles
    Gdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdnf/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdnf - by Bioz Stars, 2021-09
    94/100 stars
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    Alomone Labs cardiotropin 1
    Model. a Pridopidine triggers Neuroprotective pathways and promotes survival of SOD1 G93A MNs. b S1R targeting by pridopidine modulates axonal transport of <t>BDNF,</t> <t>GDNF</t> and mitochondria in SOD1 G93A MNs. c Pridopidine activates ERK signaling in MNs via S1R. d Pridopidine chaperones mSOD1 aggregates. e Pridopidine minimizes NMJ disruption and the subsequent muscle atrophy in SOD1 G93A models. Pridopidine also increases neurotrophic factor release from muscles
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    Image Search Results


    Model. a Pridopidine triggers Neuroprotective pathways and promotes survival of SOD1 G93A MNs. b S1R targeting by pridopidine modulates axonal transport of BDNF, GDNF and mitochondria in SOD1 G93A MNs. c Pridopidine activates ERK signaling in MNs via S1R. d Pridopidine chaperones mSOD1 aggregates. e Pridopidine minimizes NMJ disruption and the subsequent muscle atrophy in SOD1 G93A models. Pridopidine also increases neurotrophic factor release from muscles

    Journal: Cell Death & Disease

    Article Title: Targeting the Sigma-1 Receptor via Pridopidine Ameliorates Central Features of ALS Pathology in a SOD1G93A Model

    doi: 10.1038/s41419-019-1451-2

    Figure Lengend Snippet: Model. a Pridopidine triggers Neuroprotective pathways and promotes survival of SOD1 G93A MNs. b S1R targeting by pridopidine modulates axonal transport of BDNF, GDNF and mitochondria in SOD1 G93A MNs. c Pridopidine activates ERK signaling in MNs via S1R. d Pridopidine chaperones mSOD1 aggregates. e Pridopidine minimizes NMJ disruption and the subsequent muscle atrophy in SOD1 G93A models. Pridopidine also increases neurotrophic factor release from muscles

    Article Snippet: Cells were centrifuged through a 4% BSA cushion and then resuspended in Complete Neurobasal Medium (CNB) containing 2% Horse serum (Biological Industries, Ltd.), 2% B27 Supplement, 1% Glutamax, 1% Penicillin-Streptomycin, 0.5% 2-mercaptoethanol, 1 ng/mL BDNF (Alamone labs, B-250), 1 ng/mL GDNF (Alamone labs; G-240), and 0.25 ng/mL CNTF (Alamone labs; C-240).

    Techniques:

    Locally administered Pridopidine regulates the axonal transport of BDNF, GDNF, and mitochondria in co-culture of SOD1 G93A . a Schematic illustration of the experimental procedure. Primary skeletal myoblasts expressing BDNF-mCherry and GDNF-GFP are co-cultured with ventral spinal cord explants from WT or SOD1 G93A E12.5 mouse embryos. Pridopidine is exclusively applied at this phase to the distal compartment. At 7 days in co-culture, the axonal transport of muscle-derived BDNF-mCherry and GDNF-GFP, as well as the transport of mitochondria is imaged. b Representative confocal images of myocyte in the distal compartment expressing both BDNF-mCherry (red) and GDNF-GFP (green) and stained with MitoTracker (cyan). The white dashed line denotes an axon innervating a muscle containing BDNF-mCherry and GDNF-GFP particles as well as mitochondria. Scale bar: 20 µm. Lower panel includes magnified images of the axon. Yellow arrowheads denote the BDNF, GDNF, and mitochondria within the axon. Scale bar: 5 µm. c Representative time-lapse images show the retrograde axonal transport of muscle-derived BDNF-mCheery (red), GDNF-GFP (green), as well as the retrograde transport of mitochondria (cyan). White arrows denote co-transported BDNF-mCherry and GDNF-GFP. The arrowhead denotes co-transported BDNF-mCherry and mitochondria. Scale bar: 5 µm. Lower panel includes a kymograph of the entire time lapse movie demonstrating that most of the BDNF and GDNF, as well as mitochondria are retrogradely transported. Scale bars – horizontal: 5 µm vertical: 100 s. d–f Quantitative analysis of the instantaneous velocity of D) BDNF-mCherry, E) GDNF-GFP, and F) mitochondria from axons in co-culture of WT or SOD1 G93A reveals accelerated axonal transport of all of the above in SOD1 G93A compared to WT ( n = number of particle steps). Application of 0.1 µM pridopidine exclusively to the distal compartment is sufficient to restore the axonal transport properties of BDNF-mCherry and GDNF-GFP. However, the retrograde mitochondrial transport is further accelerated in the pridopidine-treated cultures. g–i Stacked distribution plots of the average velocity of G) BDNF-mCherry, H) GDNF-GFP, and I) mitochondria display higher transport velocities for all of the above in SOD1 G93A co-cultures. dashed lines highlight the changes in transporting populations of GDNF-GFP, BDNF-mCherry and mitochondria. Whereas 0.1 µM pridopidine moderates the velocities of BDNF-mCherry and GDNF-GFP, a specific population of mitochondria is transported even faster in response to the pridopidine treatment. Data are shown as the mean ± SEM. *** p value

    Journal: Cell Death & Disease

    Article Title: Targeting the Sigma-1 Receptor via Pridopidine Ameliorates Central Features of ALS Pathology in a SOD1G93A Model

    doi: 10.1038/s41419-019-1451-2

    Figure Lengend Snippet: Locally administered Pridopidine regulates the axonal transport of BDNF, GDNF, and mitochondria in co-culture of SOD1 G93A . a Schematic illustration of the experimental procedure. Primary skeletal myoblasts expressing BDNF-mCherry and GDNF-GFP are co-cultured with ventral spinal cord explants from WT or SOD1 G93A E12.5 mouse embryos. Pridopidine is exclusively applied at this phase to the distal compartment. At 7 days in co-culture, the axonal transport of muscle-derived BDNF-mCherry and GDNF-GFP, as well as the transport of mitochondria is imaged. b Representative confocal images of myocyte in the distal compartment expressing both BDNF-mCherry (red) and GDNF-GFP (green) and stained with MitoTracker (cyan). The white dashed line denotes an axon innervating a muscle containing BDNF-mCherry and GDNF-GFP particles as well as mitochondria. Scale bar: 20 µm. Lower panel includes magnified images of the axon. Yellow arrowheads denote the BDNF, GDNF, and mitochondria within the axon. Scale bar: 5 µm. c Representative time-lapse images show the retrograde axonal transport of muscle-derived BDNF-mCheery (red), GDNF-GFP (green), as well as the retrograde transport of mitochondria (cyan). White arrows denote co-transported BDNF-mCherry and GDNF-GFP. The arrowhead denotes co-transported BDNF-mCherry and mitochondria. Scale bar: 5 µm. Lower panel includes a kymograph of the entire time lapse movie demonstrating that most of the BDNF and GDNF, as well as mitochondria are retrogradely transported. Scale bars – horizontal: 5 µm vertical: 100 s. d–f Quantitative analysis of the instantaneous velocity of D) BDNF-mCherry, E) GDNF-GFP, and F) mitochondria from axons in co-culture of WT or SOD1 G93A reveals accelerated axonal transport of all of the above in SOD1 G93A compared to WT ( n = number of particle steps). Application of 0.1 µM pridopidine exclusively to the distal compartment is sufficient to restore the axonal transport properties of BDNF-mCherry and GDNF-GFP. However, the retrograde mitochondrial transport is further accelerated in the pridopidine-treated cultures. g–i Stacked distribution plots of the average velocity of G) BDNF-mCherry, H) GDNF-GFP, and I) mitochondria display higher transport velocities for all of the above in SOD1 G93A co-cultures. dashed lines highlight the changes in transporting populations of GDNF-GFP, BDNF-mCherry and mitochondria. Whereas 0.1 µM pridopidine moderates the velocities of BDNF-mCherry and GDNF-GFP, a specific population of mitochondria is transported even faster in response to the pridopidine treatment. Data are shown as the mean ± SEM. *** p value

    Article Snippet: Cells were centrifuged through a 4% BSA cushion and then resuspended in Complete Neurobasal Medium (CNB) containing 2% Horse serum (Biological Industries, Ltd.), 2% B27 Supplement, 1% Glutamax, 1% Penicillin-Streptomycin, 0.5% 2-mercaptoethanol, 1 ng/mL BDNF (Alamone labs, B-250), 1 ng/mL GDNF (Alamone labs; G-240), and 0.25 ng/mL CNTF (Alamone labs; C-240).

    Techniques: Co-Culture Assay, Expressing, Cell Culture, Derivative Assay, Staining