Journal: Conservation Physiology

Article Title: Cortisol in fish scales remains stable during extended periods of storage

doi: 10.1093/conphys/coae065

Figure Lengend Snippet: Summary results from the GCMS analysis of cortisol concentrations in scales from an individual farmed adult Atlantic salmon (contemporary scales) that were exposed to different storage conditions for a period of 12 weeks and from 120 scales from wild-caught adult Atlantic salmon that were stored dry in paper envelopes at room temperature for 3–32 years (archived scales). The contemporary scales were stored in a freezer (−20°C), at room temperature (20°C) or in the oven (50°C) for the period indicated. Confidence interval is abbreviated to CI. Values below the LOD of 3.88 ng.g −1 cortisol are excluded from the calculations

Article Snippet: One hundred milligrammes of an internal standard containing 155.4–157.3 ng.g −1 cortisol-d4 (Cerilliant) was added dropwise by weight to each sample and standard followed by 8 ml of ROMIL Methanol 215 SpS (>99.9% assay).

Techniques:

Journal: Conservation Physiology

Article Title: Cortisol in fish scales remains stable during extended periods of storage

doi: 10.1093/conphys/coae065

Figure Lengend Snippet: Cortisol concentration (ng.g −1 ) in scales from an individual farmed salmon held in an oven at 50°C for between 0 and 12 weeks. (a) shows the relationship between scale cortisol and number of weeks in the oven (R 2 = 0.1, P = 0.28). (b) shows the relationship between scale cortisol and storage temperature for scales that were held in the freezer, at room temperature or in the oven for the full 12-week period (R 2 = 0.009, P = 0.86). The dashed lines are the linear regression fits, shading indicates the 95% confidence limits of the model predictions.

Article Snippet: One hundred milligrammes of an internal standard containing 155.4–157.3 ng.g −1 cortisol-d4 (Cerilliant) was added dropwise by weight to each sample and standard followed by 8 ml of ROMIL Methanol 215 SpS (>99.9% assay).

Techniques: Concentration Assay

Journal: Conservation Physiology

Article Title: Cortisol in fish scales remains stable during extended periods of storage

doi: 10.1093/conphys/coae065

Figure Lengend Snippet: Cortisol concentration (ng.g −1 ) 0.5 in scales of 107 wild-caught adult salmon sampled between 1989 and 2018 and analysed in 2021. Lightly shaded points indicate the individual measurements, dark shaded points indicate the mean values for each year. Whiskers indicate the 95% confidence intervals of the mean values. The dashed line is a linear regression of the relationship between cortisol concentration and years in storage (R 2 = 0.003, P = 0.58); shading indicates the 95% confidence limits of the model predictions. The square root transform was applied to normalize the distribution of the data for regression analysis. Thirteen cortisol measurements that were below the limit of detection of 3.88 ng.g −1 were excluded from the plot.

Article Snippet: One hundred milligrammes of an internal standard containing 155.4–157.3 ng.g −1 cortisol-d4 (Cerilliant) was added dropwise by weight to each sample and standard followed by 8 ml of ROMIL Methanol 215 SpS (>99.9% assay).

Techniques: Concentration Assay