vu0529331 (Alomone Labs)


Structured Review

Vu0529331, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vu0529331/product/Alomone Labs
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)"
Article Title: A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)
Journal: bioRxiv
doi: 10.1101/866202

Figure Legend Snippet: The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
Techniques Used: Mutagenesis
2) Product Images from "A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)"
Article Title: A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)
Journal: bioRxiv
doi: 10.1101/866202

Figure Legend Snippet: The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
Techniques Used: Mutagenesis
3) Product Images from "Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels"
Article Title: Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels
Journal: iScience
doi: 10.1016/j.isci.2021.103018

Figure Legend Snippet: Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).
Techniques Used: Activity Assay, Activation Assay, Expressing