gv 58  (Alomone Labs)


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    Structured Review

    Alomone Labs gv 58
    Enhanced presynaptic Ca 2+ influx rescues evoked release in BoNT/A-intoxicated cultures. ( A ) Representative whole-cell recordings of evoked neurotransmission in non-intoxicated cultures treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or <t>GV-58</t> (50 µM). ( B ) Mean EPSC amplitudes from non-intoxicated cultures. ( B ) Representative whole-cell recordings of evoked neurotransmission in BoNT/A-intoxicated neurons treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( D ) Mean EPSC amplitudes for BoNT/A-intoxicated cultures. EPSC amplitudes are presented as mean ± SEM and n ≥ 12 recordings for all conditions. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Arrows represent stimulation times. *Indicated p
    Gv 58, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gv 58/product/Alomone Labs
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    gv 58 - by Bioz Stars, 2022-12
    88/100 stars

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    1) Product Images from "Use-dependent potentiation of voltage-gated calcium channels rescues neurotransmission in nerve terminals intoxicated by botulinum neurotoxin serotype A"

    Article Title: Use-dependent potentiation of voltage-gated calcium channels rescues neurotransmission in nerve terminals intoxicated by botulinum neurotoxin serotype A

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16064-3

    Enhanced presynaptic Ca 2+ influx rescues evoked release in BoNT/A-intoxicated cultures. ( A ) Representative whole-cell recordings of evoked neurotransmission in non-intoxicated cultures treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( B ) Mean EPSC amplitudes from non-intoxicated cultures. ( B ) Representative whole-cell recordings of evoked neurotransmission in BoNT/A-intoxicated neurons treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( D ) Mean EPSC amplitudes for BoNT/A-intoxicated cultures. EPSC amplitudes are presented as mean ± SEM and n ≥ 12 recordings for all conditions. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Arrows represent stimulation times. *Indicated p
    Figure Legend Snippet: Enhanced presynaptic Ca 2+ influx rescues evoked release in BoNT/A-intoxicated cultures. ( A ) Representative whole-cell recordings of evoked neurotransmission in non-intoxicated cultures treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( B ) Mean EPSC amplitudes from non-intoxicated cultures. ( B ) Representative whole-cell recordings of evoked neurotransmission in BoNT/A-intoxicated neurons treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( D ) Mean EPSC amplitudes for BoNT/A-intoxicated cultures. EPSC amplitudes are presented as mean ± SEM and n ≥ 12 recordings for all conditions. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Arrows represent stimulation times. *Indicated p

    Techniques Used:

    Use-dependent L-type or N/P/Q-type VGCC agonists restore spontaneous synaptic activity to neuronal cultures fully intoxicated by BoNT/A in physiological Ca 2+ . ( A ) Representative whole-cell recordings and mean mEPSC frequencies in non-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). ( B ) Representative whole-cell recordings and mean mEPSC frequencies in BoNT/A-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). mEPSC frequencies are normalized to recordings from age-matched, non-intoxicated control cultures. Scale bars represent 5 s (x-axis) and 40 pA (y-axis). All data presented as mean ± SEM and n ≥ 12 neurons for all conditions. *Indicates p
    Figure Legend Snippet: Use-dependent L-type or N/P/Q-type VGCC agonists restore spontaneous synaptic activity to neuronal cultures fully intoxicated by BoNT/A in physiological Ca 2+ . ( A ) Representative whole-cell recordings and mean mEPSC frequencies in non-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). ( B ) Representative whole-cell recordings and mean mEPSC frequencies in BoNT/A-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). mEPSC frequencies are normalized to recordings from age-matched, non-intoxicated control cultures. Scale bars represent 5 s (x-axis) and 40 pA (y-axis). All data presented as mean ± SEM and n ≥ 12 neurons for all conditions. *Indicates p

    Techniques Used: Activity Assay

    3,4-DAP plus GV-58 restores evoked neurotransmission to baseline levels in cultured neurons intoxicated by BoNT/A, but not BoNT/D or BoNT/E. Representative EPSC and mean EPSC amplitudes from cultures intoxicated with: ( A ) vehicle; ( B ) BoNT/A; ( C ) BoNT/D; or ( D ) BoNT/E. Arrows represent stimulation times. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Mean EPSC amplitudes are presented as mean ± SEM; n ≥ 13 recordings for 5A-B and n ≥ 9 recordings for 5C–D. *Indicates p
    Figure Legend Snippet: 3,4-DAP plus GV-58 restores evoked neurotransmission to baseline levels in cultured neurons intoxicated by BoNT/A, but not BoNT/D or BoNT/E. Representative EPSC and mean EPSC amplitudes from cultures intoxicated with: ( A ) vehicle; ( B ) BoNT/A; ( C ) BoNT/D; or ( D ) BoNT/E. Arrows represent stimulation times. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Mean EPSC amplitudes are presented as mean ± SEM; n ≥ 13 recordings for 5A-B and n ≥ 9 recordings for 5C–D. *Indicates p

    Techniques Used: Cell Culture

    3,4-DAP plus GV-58 has superadditive effects in restoring robust EPP production in mouse diaphragm endplates fully intoxicated by BoNT/A. Representative evoked endplate recordings (top) and corresponding mean EPP amplitudes (in mV, bottom) from non-intoxicated and BoNT/A-intoxicated mouse phrenic nerve-hemidiaphragm preparations treated with vehicle, GV-58 (50 µM), 3,4-DAP (2 µM), or GV-58 plus 3,4-DAP (50 µM and 2 µM, respectively); and BoNT/E-intoxicated tissue treated with vehicle, or GV-58 plus 3,4-DAP. Representative traces are the average of six EPPs elicited from single endplates. Scale bars represent 25 ms (x-axis) and 5 mV (y-axis). Grouped data is presented as mean ± SEM (n = 4–6 diaphragm per condition; 5 endplates recorded per diaphragm). ***Indicates p
    Figure Legend Snippet: 3,4-DAP plus GV-58 has superadditive effects in restoring robust EPP production in mouse diaphragm endplates fully intoxicated by BoNT/A. Representative evoked endplate recordings (top) and corresponding mean EPP amplitudes (in mV, bottom) from non-intoxicated and BoNT/A-intoxicated mouse phrenic nerve-hemidiaphragm preparations treated with vehicle, GV-58 (50 µM), 3,4-DAP (2 µM), or GV-58 plus 3,4-DAP (50 µM and 2 µM, respectively); and BoNT/E-intoxicated tissue treated with vehicle, or GV-58 plus 3,4-DAP. Representative traces are the average of six EPPs elicited from single endplates. Scale bars represent 25 ms (x-axis) and 5 mV (y-axis). Grouped data is presented as mean ± SEM (n = 4–6 diaphragm per condition; 5 endplates recorded per diaphragm). ***Indicates p

    Techniques Used:

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    Alomone Labs gv 58
    Enhanced presynaptic Ca 2+ influx rescues evoked release in BoNT/A-intoxicated cultures. ( A ) Representative whole-cell recordings of evoked neurotransmission in non-intoxicated cultures treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or <t>GV-58</t> (50 µM). ( B ) Mean EPSC amplitudes from non-intoxicated cultures. ( B ) Representative whole-cell recordings of evoked neurotransmission in BoNT/A-intoxicated neurons treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( D ) Mean EPSC amplitudes for BoNT/A-intoxicated cultures. EPSC amplitudes are presented as mean ± SEM and n ≥ 12 recordings for all conditions. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Arrows represent stimulation times. *Indicated p
    Gv 58, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gv 58/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gv 58 - by Bioz Stars, 2022-12
    88/100 stars
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    Enhanced presynaptic Ca 2+ influx rescues evoked release in BoNT/A-intoxicated cultures. ( A ) Representative whole-cell recordings of evoked neurotransmission in non-intoxicated cultures treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( B ) Mean EPSC amplitudes from non-intoxicated cultures. ( B ) Representative whole-cell recordings of evoked neurotransmission in BoNT/A-intoxicated neurons treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( D ) Mean EPSC amplitudes for BoNT/A-intoxicated cultures. EPSC amplitudes are presented as mean ± SEM and n ≥ 12 recordings for all conditions. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Arrows represent stimulation times. *Indicated p

    Journal: Scientific Reports

    Article Title: Use-dependent potentiation of voltage-gated calcium channels rescues neurotransmission in nerve terminals intoxicated by botulinum neurotoxin serotype A

    doi: 10.1038/s41598-017-16064-3

    Figure Lengend Snippet: Enhanced presynaptic Ca 2+ influx rescues evoked release in BoNT/A-intoxicated cultures. ( A ) Representative whole-cell recordings of evoked neurotransmission in non-intoxicated cultures treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( B ) Mean EPSC amplitudes from non-intoxicated cultures. ( B ) Representative whole-cell recordings of evoked neurotransmission in BoNT/A-intoxicated neurons treated with vehicle, 16 mM Ca 2+ , FPL 64176 (10 µM), or GV-58 (50 µM). ( D ) Mean EPSC amplitudes for BoNT/A-intoxicated cultures. EPSC amplitudes are presented as mean ± SEM and n ≥ 12 recordings for all conditions. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Arrows represent stimulation times. *Indicated p

    Article Snippet: For BoNT/E, toxin was activated by a 60 min incubation at 37 °C with 0.3 mg/mL TPCK-treated trypsin in 0.05 M sodium phosphate buffer (pH 6.5) . ω-agatoxin IVA, ω-conotoxin MVIIC and GV-58 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques:

    Use-dependent L-type or N/P/Q-type VGCC agonists restore spontaneous synaptic activity to neuronal cultures fully intoxicated by BoNT/A in physiological Ca 2+ . ( A ) Representative whole-cell recordings and mean mEPSC frequencies in non-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). ( B ) Representative whole-cell recordings and mean mEPSC frequencies in BoNT/A-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). mEPSC frequencies are normalized to recordings from age-matched, non-intoxicated control cultures. Scale bars represent 5 s (x-axis) and 40 pA (y-axis). All data presented as mean ± SEM and n ≥ 12 neurons for all conditions. *Indicates p

    Journal: Scientific Reports

    Article Title: Use-dependent potentiation of voltage-gated calcium channels rescues neurotransmission in nerve terminals intoxicated by botulinum neurotoxin serotype A

    doi: 10.1038/s41598-017-16064-3

    Figure Lengend Snippet: Use-dependent L-type or N/P/Q-type VGCC agonists restore spontaneous synaptic activity to neuronal cultures fully intoxicated by BoNT/A in physiological Ca 2+ . ( A ) Representative whole-cell recordings and mean mEPSC frequencies in non-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). ( B ) Representative whole-cell recordings and mean mEPSC frequencies in BoNT/A-intoxicated cultures treated with vehicle, FPL 64176 (10 µM) or GV-58 (50 µM). mEPSC frequencies are normalized to recordings from age-matched, non-intoxicated control cultures. Scale bars represent 5 s (x-axis) and 40 pA (y-axis). All data presented as mean ± SEM and n ≥ 12 neurons for all conditions. *Indicates p

    Article Snippet: For BoNT/E, toxin was activated by a 60 min incubation at 37 °C with 0.3 mg/mL TPCK-treated trypsin in 0.05 M sodium phosphate buffer (pH 6.5) . ω-agatoxin IVA, ω-conotoxin MVIIC and GV-58 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay

    3,4-DAP plus GV-58 restores evoked neurotransmission to baseline levels in cultured neurons intoxicated by BoNT/A, but not BoNT/D or BoNT/E. Representative EPSC and mean EPSC amplitudes from cultures intoxicated with: ( A ) vehicle; ( B ) BoNT/A; ( C ) BoNT/D; or ( D ) BoNT/E. Arrows represent stimulation times. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Mean EPSC amplitudes are presented as mean ± SEM; n ≥ 13 recordings for 5A-B and n ≥ 9 recordings for 5C–D. *Indicates p

    Journal: Scientific Reports

    Article Title: Use-dependent potentiation of voltage-gated calcium channels rescues neurotransmission in nerve terminals intoxicated by botulinum neurotoxin serotype A

    doi: 10.1038/s41598-017-16064-3

    Figure Lengend Snippet: 3,4-DAP plus GV-58 restores evoked neurotransmission to baseline levels in cultured neurons intoxicated by BoNT/A, but not BoNT/D or BoNT/E. Representative EPSC and mean EPSC amplitudes from cultures intoxicated with: ( A ) vehicle; ( B ) BoNT/A; ( C ) BoNT/D; or ( D ) BoNT/E. Arrows represent stimulation times. Scale bars represent 400 ms (x-axis) and 100 pA (y-axis). Mean EPSC amplitudes are presented as mean ± SEM; n ≥ 13 recordings for 5A-B and n ≥ 9 recordings for 5C–D. *Indicates p

    Article Snippet: For BoNT/E, toxin was activated by a 60 min incubation at 37 °C with 0.3 mg/mL TPCK-treated trypsin in 0.05 M sodium phosphate buffer (pH 6.5) . ω-agatoxin IVA, ω-conotoxin MVIIC and GV-58 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Cell Culture

    3,4-DAP plus GV-58 has superadditive effects in restoring robust EPP production in mouse diaphragm endplates fully intoxicated by BoNT/A. Representative evoked endplate recordings (top) and corresponding mean EPP amplitudes (in mV, bottom) from non-intoxicated and BoNT/A-intoxicated mouse phrenic nerve-hemidiaphragm preparations treated with vehicle, GV-58 (50 µM), 3,4-DAP (2 µM), or GV-58 plus 3,4-DAP (50 µM and 2 µM, respectively); and BoNT/E-intoxicated tissue treated with vehicle, or GV-58 plus 3,4-DAP. Representative traces are the average of six EPPs elicited from single endplates. Scale bars represent 25 ms (x-axis) and 5 mV (y-axis). Grouped data is presented as mean ± SEM (n = 4–6 diaphragm per condition; 5 endplates recorded per diaphragm). ***Indicates p

    Journal: Scientific Reports

    Article Title: Use-dependent potentiation of voltage-gated calcium channels rescues neurotransmission in nerve terminals intoxicated by botulinum neurotoxin serotype A

    doi: 10.1038/s41598-017-16064-3

    Figure Lengend Snippet: 3,4-DAP plus GV-58 has superadditive effects in restoring robust EPP production in mouse diaphragm endplates fully intoxicated by BoNT/A. Representative evoked endplate recordings (top) and corresponding mean EPP amplitudes (in mV, bottom) from non-intoxicated and BoNT/A-intoxicated mouse phrenic nerve-hemidiaphragm preparations treated with vehicle, GV-58 (50 µM), 3,4-DAP (2 µM), or GV-58 plus 3,4-DAP (50 µM and 2 µM, respectively); and BoNT/E-intoxicated tissue treated with vehicle, or GV-58 plus 3,4-DAP. Representative traces are the average of six EPPs elicited from single endplates. Scale bars represent 25 ms (x-axis) and 5 mV (y-axis). Grouped data is presented as mean ± SEM (n = 4–6 diaphragm per condition; 5 endplates recorded per diaphragm). ***Indicates p

    Article Snippet: For BoNT/E, toxin was activated by a 60 min incubation at 37 °C with 0.3 mg/mL TPCK-treated trypsin in 0.05 M sodium phosphate buffer (pH 6.5) . ω-agatoxin IVA, ω-conotoxin MVIIC and GV-58 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: