ngf  (Alomone Labs)


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  • 80
    Name:
    NGF Blocking Peptide
    Description:
    A Blocking Peptide for Anti NGF Antibody
    Catalog Number:
    BLP-N240
    Price:
    245.0
    Category:
    Control reagent Negative Control
    Source:
    Natural protein
    Applications:
    Blocking
    Purity:
    >95% (HPLC)
    Size:
    50 mcg
    Format:
    Lyophilized powder
    Buy from Supplier


    Structured Review

    Alomone Labs ngf
    NGF Blocking Peptide
    A Blocking Peptide for Anti NGF Antibody
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2021-09
    80/100 stars

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    Related Articles

    Transfection:

    Article Title: Interaction between the transmembrane domains of neurotrophin receptors p75 and TrkA mediates their reciprocal activation
    Article Snippet: .. About 48 h after transfection, the cells were starved with serum-free medium for 2 h and stimulated with NGF (Alomone) at the indicated concentrations and time intervals. ..

    Cell Culture:

    Article Title: Cinnamaldehyde elicits itch behavior via TRPV1 and TRPV4 but not TRPA1
    Article Snippet: .. NGF at 100 ng/mL (Alomone Labs, Tel Aviv, Israel) was added and cells were cultured for 16–24 hours. ..

    Article Title: Extracellular vesicles from neuronal cells promote neural induction of mESCs through cyclinD1
    Article Snippet: .. Differentiated PC12 cells were cultured at a density of 5.5-6.5× 104 /cm2 , in DMEM medium supplemented with NGF (50ng/ml, Alomone Labs, Jerusalem, Israel), 1% FBS. ..

    Article Title: Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1
    Article Snippet: .. Differentiated PC12 cells were cultured at a density of 5.5–6.5 × 104 /cm2 in DMEM medium supplemented with NGF (50 ng/ml; Alomone Labs) and 1% FBS. ..

    other:

    Article Title: DDHD1, but Not DDHD2, Suppresses Neurite Outgrowth in SH-SY5Y and PC12 Cells by Regulating Protein Transport From Recycling Endosomes
    Article Snippet: The following reagents were used: NGF (Alomone Labs); RA, unlabeled-Tfn, deferoxamine, R59949, FITC-phalloidin and TRITC-phalloidin (Sigma-Aldrich); CAY1059 and CAY10594 (Cayman Chemical); Alexa488-Tfn and TRITC-Tfn (Thermo Fisher Scientific); and EGF (Invitrogen).

    Positive Control:

    Article Title: Human adipose-derived mesenchymal stem cell-conditioned medium ameliorates polyneuropathy and foot ulceration in diabetic BKS db/db mice
    Article Snippet: .. As positive control for neuritogenesis, samples were incubated with 10 ng/ml NGF (Alamone Labs, Israel). ..

    Incubation:

    Article Title: Human adipose-derived mesenchymal stem cell-conditioned medium ameliorates polyneuropathy and foot ulceration in diabetic BKS db/db mice
    Article Snippet: .. As positive control for neuritogenesis, samples were incubated with 10 ng/ml NGF (Alamone Labs, Israel). ..

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  • 93
    Alomone Labs kv2 1 antibody
    Postnatal development of the <t>Kv2.1</t> and Kv2.2 mRNA levels in dorsal root ganglion ( DRG ) cultures. Electrophoretic analysis of the RT ‐ PCR products obtained from cultured DRG neurons of 1 week, 2 weeks, 3 weeks, and 4 weeks old mice. The relative densitometric values ( RDV s) (presented in panel C) were determined by normalizing the densitometric value of the 450 bp fragment, which corresponds to Kv2.1 (A) and Kv2.2 (B), to the densitometric value of G3 PDH , which corresponds to the 250 bp fragment in both panels. (C) The RDV of Kv2.2 (white) decreased significantly determined in DRG cultures from 3 and 4 weeks old mice compared to the RDV determined in DRG cultures from 1‐week‐old mice (* P
    Kv2 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv2 1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kv2 1 antibody - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    86
    Alomone Labs kt 5823
    Effects of BPA on [Ca 2+ ] i oscillations through a PKG-mediated mechanism. ( A ) Low-glucose–induced [Ca 2+ ] i oscillations blocked by 1 nM BPA. ( B ) Frequency of [Ca 2+ ] i oscillations were not reduced by BPA in an islet from the same preparation and maintained in the same conditions but pretreated with and exposed to the PKG inhibitor <t>KT-5823</t> (1 μM). ( C ) Mean frequency values of 0.5 mM glucose before application of either BPA (G1) or E 2 (G2), in the presence of 1 nM BPA or 1 nM E 2 , as in ( A ) , or in the presence of 1 μM KT-5823 plus 0.5 mM glucose (KT); KT plus 1 nM BPA (BPA + KT), and KT plus 1 nM 17β-E 2 (E 2 + KT) as in ( B ). Results are representative of at least 12 cells from nine different islets, expressed as mean ± SE. * p
    Kt 5823, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kt 5823/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kt 5823 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    93
    Alomone Labs rabbit polyclonal anti p2x1
    Effects of BPA on [Ca 2+ ] i oscillations through a PKG-mediated mechanism. ( A ) Low-glucose–induced [Ca 2+ ] i oscillations blocked by 1 nM BPA. ( B ) Frequency of [Ca 2+ ] i oscillations were not reduced by BPA in an islet from the same preparation and maintained in the same conditions but pretreated with and exposed to the PKG inhibitor <t>KT-5823</t> (1 μM). ( C ) Mean frequency values of 0.5 mM glucose before application of either BPA (G1) or E 2 (G2), in the presence of 1 nM BPA or 1 nM E 2 , as in ( A ) , or in the presence of 1 μM KT-5823 plus 0.5 mM glucose (KT); KT plus 1 nM BPA (BPA + KT), and KT plus 1 nM 17β-E 2 (E 2 + KT) as in ( B ). Results are representative of at least 12 cells from nine different islets, expressed as mean ± SE. * p
    Rabbit Polyclonal Anti P2x1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti p2x1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti p2x1 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Postnatal development of the Kv2.1 and Kv2.2 mRNA levels in dorsal root ganglion ( DRG ) cultures. Electrophoretic analysis of the RT ‐ PCR products obtained from cultured DRG neurons of 1 week, 2 weeks, 3 weeks, and 4 weeks old mice. The relative densitometric values ( RDV s) (presented in panel C) were determined by normalizing the densitometric value of the 450 bp fragment, which corresponds to Kv2.1 (A) and Kv2.2 (B), to the densitometric value of G3 PDH , which corresponds to the 250 bp fragment in both panels. (C) The RDV of Kv2.2 (white) decreased significantly determined in DRG cultures from 3 and 4 weeks old mice compared to the RDV determined in DRG cultures from 1‐week‐old mice (* P

    Journal: Physiological Reports

    Article Title: The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons. The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons

    doi: 10.14814/phy2.12731

    Figure Lengend Snippet: Postnatal development of the Kv2.1 and Kv2.2 mRNA levels in dorsal root ganglion ( DRG ) cultures. Electrophoretic analysis of the RT ‐ PCR products obtained from cultured DRG neurons of 1 week, 2 weeks, 3 weeks, and 4 weeks old mice. The relative densitometric values ( RDV s) (presented in panel C) were determined by normalizing the densitometric value of the 450 bp fragment, which corresponds to Kv2.1 (A) and Kv2.2 (B), to the densitometric value of G3 PDH , which corresponds to the 250 bp fragment in both panels. (C) The RDV of Kv2.2 (white) decreased significantly determined in DRG cultures from 3 and 4 weeks old mice compared to the RDV determined in DRG cultures from 1‐week‐old mice (* P

    Article Snippet: For the anti‐Kv2.1 current recordings, patch pipettes were dipped in normal intracellular solution and back filled with the anti‐Kv2.1‐containing solution obtained by dissolving 10 μ g/mL Kv2.1 antibody (Alomone Labs) in the intracellular solution.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Mouse Assay

    Postnatal development of anti‐Kv2.1‐sensitive current in dorsal root ganglion ( DRG ) neurons at 0 mV . (A) Representative current recordings of the total outward K + (left), anti‐Kv2.1‐insensitive (right) and anti‐Kv2.1‐sensitive (bottom) currents in DRG neurons obtained from 1 week (gray) and 4 weeks (black) old mice elicited by a 500 msec depolarizing pulse to 0 mV from a holding potential of −70 mV . The anti‐Kv2.1 sensitive current was obtained by subtracting the current after intracellular diffusion of Kv2.1 antibodies (i.e., anti‐Kv2.1‐insensitive current) from the total outward K + current. The scale bar applies to all current recordings. (B) Current densities of the anti‐Kv2.1‐sensitive component in the different age groups. The anti‐Kv2.1‐sensitive current density rose gradually, although not significantly, during postnatal development. (C) The fraction of the anti‐Kv2.1‐sensitive current relative to I K at the different developmental stages obtained as described in the Results section remained unchanged. The numbers above each bar indicate the number of cells analyzed.

    Journal: Physiological Reports

    Article Title: The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons. The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons

    doi: 10.14814/phy2.12731

    Figure Lengend Snippet: Postnatal development of anti‐Kv2.1‐sensitive current in dorsal root ganglion ( DRG ) neurons at 0 mV . (A) Representative current recordings of the total outward K + (left), anti‐Kv2.1‐insensitive (right) and anti‐Kv2.1‐sensitive (bottom) currents in DRG neurons obtained from 1 week (gray) and 4 weeks (black) old mice elicited by a 500 msec depolarizing pulse to 0 mV from a holding potential of −70 mV . The anti‐Kv2.1 sensitive current was obtained by subtracting the current after intracellular diffusion of Kv2.1 antibodies (i.e., anti‐Kv2.1‐insensitive current) from the total outward K + current. The scale bar applies to all current recordings. (B) Current densities of the anti‐Kv2.1‐sensitive component in the different age groups. The anti‐Kv2.1‐sensitive current density rose gradually, although not significantly, during postnatal development. (C) The fraction of the anti‐Kv2.1‐sensitive current relative to I K at the different developmental stages obtained as described in the Results section remained unchanged. The numbers above each bar indicate the number of cells analyzed.

    Article Snippet: For the anti‐Kv2.1 current recordings, patch pipettes were dipped in normal intracellular solution and back filled with the anti‐Kv2.1‐containing solution obtained by dissolving 10 μ g/mL Kv2.1 antibody (Alomone Labs) in the intracellular solution.

    Techniques: Mouse Assay, Diffusion-based Assay

    Postnatal development of the ScTx‐ and anti‐Kv2.1‐sensitive current at +20 and +40 mV . The fraction of ScTx‐sensitive current (left) and anti‐Kv2.1‐sensitive current (right) relative to I K of the different postnatal age groups at the end of a 500 msec depolarizing pulse at +20 mV (A) and +40 mV (B). (A) At +20 mV , the fraction of ScTx‐sensitive current in dorsal root ganglion neurons from 1 week old mice was significantly larger compared to that from 4 week old mice (* P

    Journal: Physiological Reports

    Article Title: The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons. The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons

    doi: 10.14814/phy2.12731

    Figure Lengend Snippet: Postnatal development of the ScTx‐ and anti‐Kv2.1‐sensitive current at +20 and +40 mV . The fraction of ScTx‐sensitive current (left) and anti‐Kv2.1‐sensitive current (right) relative to I K of the different postnatal age groups at the end of a 500 msec depolarizing pulse at +20 mV (A) and +40 mV (B). (A) At +20 mV , the fraction of ScTx‐sensitive current in dorsal root ganglion neurons from 1 week old mice was significantly larger compared to that from 4 week old mice (* P

    Article Snippet: For the anti‐Kv2.1 current recordings, patch pipettes were dipped in normal intracellular solution and back filled with the anti‐Kv2.1‐containing solution obtained by dissolving 10 μ g/mL Kv2.1 antibody (Alomone Labs) in the intracellular solution.

    Techniques: Mouse Assay

    Effects of BPA on [Ca 2+ ] i oscillations through a PKG-mediated mechanism. ( A ) Low-glucose–induced [Ca 2+ ] i oscillations blocked by 1 nM BPA. ( B ) Frequency of [Ca 2+ ] i oscillations were not reduced by BPA in an islet from the same preparation and maintained in the same conditions but pretreated with and exposed to the PKG inhibitor KT-5823 (1 μM). ( C ) Mean frequency values of 0.5 mM glucose before application of either BPA (G1) or E 2 (G2), in the presence of 1 nM BPA or 1 nM E 2 , as in ( A ) , or in the presence of 1 μM KT-5823 plus 0.5 mM glucose (KT); KT plus 1 nM BPA (BPA + KT), and KT plus 1 nM 17β-E 2 (E 2 + KT) as in ( B ). Results are representative of at least 12 cells from nine different islets, expressed as mean ± SE. * p

    Journal: Environmental Health Perspectives

    Article Title: Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca2+ Signals in Pancreatic ?-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans

    doi: 10.1289/ehp.8002

    Figure Lengend Snippet: Effects of BPA on [Ca 2+ ] i oscillations through a PKG-mediated mechanism. ( A ) Low-glucose–induced [Ca 2+ ] i oscillations blocked by 1 nM BPA. ( B ) Frequency of [Ca 2+ ] i oscillations were not reduced by BPA in an islet from the same preparation and maintained in the same conditions but pretreated with and exposed to the PKG inhibitor KT-5823 (1 μM). ( C ) Mean frequency values of 0.5 mM glucose before application of either BPA (G1) or E 2 (G2), in the presence of 1 nM BPA or 1 nM E 2 , as in ( A ) , or in the presence of 1 μM KT-5823 plus 0.5 mM glucose (KT); KT plus 1 nM BPA (BPA + KT), and KT plus 1 nM 17β-E 2 (E 2 + KT) as in ( B ). Results are representative of at least 12 cells from nine different islets, expressed as mean ± SE. * p

    Article Snippet: Thus, KT-5823 does not alter [Ca2+ ]i oscillations but prevents the suppression of low-glucose–induced [Ca2+ ]i oscillations by BPA, indicating that BPA’s effect is exerted by a cGMP/PKG-mediated mechanism, as has been demonstrated for the natural hormone 17β-E2 ( ).

    Techniques:

    Effects of 8Br-cGMP on EDCs and E 2 via PKG. ( A ) Exposure to 10 μM 8Br-cGMP dramatically reduces the frequency of low-glucose–induced [Ca 2+ ] i oscillations. ( B ) After incubation with the specific PKG inhibitor KT-5823 (1 μM), 8Br-cGMP fails to evoke the marked reduction in [Ca 2+ ] i oscillations shown in ( A ). ( C ) Mean frequency values collected in the presence of 0.5 mM glucose (G), 8Br-cGMP plus 0.5 mM glucose (8Br-cGMP), KT-5823 (KT), and 8Br-cGMP plus KT-5823 (8Br-cGMP + KT). Results are representative of at least five cells in four different islets, expressed as mean ± SE.

    Journal: Environmental Health Perspectives

    Article Title: Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca2+ Signals in Pancreatic ?-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans

    doi: 10.1289/ehp.8002

    Figure Lengend Snippet: Effects of 8Br-cGMP on EDCs and E 2 via PKG. ( A ) Exposure to 10 μM 8Br-cGMP dramatically reduces the frequency of low-glucose–induced [Ca 2+ ] i oscillations. ( B ) After incubation with the specific PKG inhibitor KT-5823 (1 μM), 8Br-cGMP fails to evoke the marked reduction in [Ca 2+ ] i oscillations shown in ( A ). ( C ) Mean frequency values collected in the presence of 0.5 mM glucose (G), 8Br-cGMP plus 0.5 mM glucose (8Br-cGMP), KT-5823 (KT), and 8Br-cGMP plus KT-5823 (8Br-cGMP + KT). Results are representative of at least five cells in four different islets, expressed as mean ± SE.

    Article Snippet: Thus, KT-5823 does not alter [Ca2+ ]i oscillations but prevents the suppression of low-glucose–induced [Ca2+ ]i oscillations by BPA, indicating that BPA’s effect is exerted by a cGMP/PKG-mediated mechanism, as has been demonstrated for the natural hormone 17β-E2 ( ).

    Techniques: Incubation