Journal: Physiological Reports
Article Title: The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons. The contribution of Kv2.2‐mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons
Figure Lengend Snippet: Postnatal development of the Kv2.1 and Kv2.2 mRNA levels in dorsal root ganglion ( DRG ) cultures. Electrophoretic analysis of the RT ‐ PCR products obtained from cultured DRG neurons of 1 week, 2 weeks, 3 weeks, and 4 weeks old mice. The relative densitometric values ( RDV s) (presented in panel C) were determined by normalizing the densitometric value of the 450 bp fragment, which corresponds to Kv2.1 (A) and Kv2.2 (B), to the densitometric value of G3 PDH , which corresponds to the 250 bp fragment in both panels. (C) The RDV of Kv2.2 (white) decreased significantly determined in DRG cultures from 3 and 4 weeks old mice compared to the RDV determined in DRG cultures from 1‐week‐old mice (* P
Article Snippet: For the anti‐Kv2.1 current recordings, patch pipettes were dipped in normal intracellular solution and back filled with the anti‐Kv2.1‐containing solution obtained by dissolving 10 μ g/mL Kv2.1 antibody (Alomone Labs) in the intracellular solution.
Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Mouse Assay