Journal: The Journal of Headache and Pain

Article Title: Estrogen receptors α, β and GPER in the CNS and trigeminal system - molecular and functional aspects

doi: 10.1186/s10194-020-01197-0

Figure Lengend Snippet: Details of primary and secondary antibodies

Article Snippet: GPER (G protein-coupled estrogen receptor 1) , 1:100 , rabbit , (C) ETFRDKLRLYVAQK, corresponding to amino acid residues 329–342 , Alomone Labs, Ltd., Jerusalem, Israel.

Techniques: Recombinant

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Evaluation of Long-Term Cryostorage of Brain Tissue Sections for Quantitative Histochemistry

doi: 10.1369/0022155416686934

Figure Lengend Snippet: Simple linear correlations between TTF and stereological cell counts. TTF in years versus stereological counts for the following antigens: (A) NeuN, a marker of neurons, counted in cortical area 17; (B) orexin-A, an excitatory neuropeptide hormone produced by a subpopulation of neurons, counted in hypothalamus; (C) parvalbumin, a calcium-binding protein used to label a subpopulation of GABAergic interneurons, counted in cortical area 17; (D) BrdU, a marker of newly generated cells, counted in dentate gyrus; (E) DCX, a marker of immature neurons and neuronal precursors, counted in dentate gyrus. Abbreviations: TTF, total time frozen; NeuN, neuronal nuclei; GABA, gamma-aminobutyric acid; BrdU, bromodeoxyuridine; DCX, doublecortin; PV, parvalbumin; DCX, doublecortin.

Article Snippet: Buffer washes (potassium phosphate–buffered saline [KPBS], PBS, PB, or TBS, pH 6.8–7.4) were interposed between all basic steps. table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Dataset Antigen Antibody Company NeuN (clone A60) Soluble nuclear protein in neurons, 46-kDa and 48-kDa isoforms Mouse monoclonal MAB377, lot LV1388597; Chemicon, Temecula, CA PV Calcium-binding protein found in GABAergic interneurons Mouse monoclonal PV235; Swant, Marly, Switzerland Orexin-A (C-19) Excitatory neuropeptide hormone Goat polyclonal SC-8070; Santa Cruz Biotechnology, Dallas, TX BrdU Synthetic analogue of thymidine; nuclear localization Mouse monoclonal Chemicon dMBP Amino acids 69 to 86 of guinea pig myelin basic protein Rabbit polyclonal AB5864, lot 2382586; Millipore, Billerica, MA DCX Microtubule-associated protein Rabbit polyclonal #4604; Cell Signaling Technology, Danvers, MA HA HA, the major glycosaminoglycan component of neural extracellular matrix NA (cow-derived HA-binding protein) #385911, lot D00155144; Millipore proBDNF Precursor of BDNF, a member of the neurotrophin family of growth receptors Rabbit polyclonal ANT-006, lot AN-05; Alomone Labs, Jerusalem, Israel Open in a separate window Abbreviations: NeuN, neuronal nuclei; PV, parvalbumin; GABA, gamma-aminobutyric acid; BrdU, bromodeoxyuridine; dMBP, damaged myelin basic protein; DCX, doublecortin; HA, hyaluronic acid; proBDNF, pro-brain-derived neurotrophic factor.

Techniques: Marker, Produced, Binding Assay, Generated