rt cdna synthesis mix  (Solis BioDyne)


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    Solis BioDyne rt cdna synthesis mix
    Generation of hsd11b2 mutant zebrafish line. ( A ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. ( B ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. ( C ) Representative picture of hsd11b2 +/+ , hsd11b2 +/− and hsd11b2 −/− genotyping in 2% agarose gel. ( D ) <t>RT-qPCR</t> of hsd11b2 transcripts in hsd11b2 +/+ and hsd11b2 −/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t -test. * p
    Rt Cdna Synthesis Mix, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt cdna synthesis mix/product/Solis BioDyne
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt cdna synthesis mix - by Bioz Stars, 2022-07
    96/100 stars

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    1) Product Images from "Knockout of the hsd11b2 Gene Extends the Cortisol Stress Response in Both Zebrafish Larvae and Adults"

    Article Title: Knockout of the hsd11b2 Gene Extends the Cortisol Stress Response in Both Zebrafish Larvae and Adults

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms222212525

    Generation of hsd11b2 mutant zebrafish line. ( A ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. ( B ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. ( C ) Representative picture of hsd11b2 +/+ , hsd11b2 +/− and hsd11b2 −/− genotyping in 2% agarose gel. ( D ) RT-qPCR of hsd11b2 transcripts in hsd11b2 +/+ and hsd11b2 −/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t -test. * p
    Figure Legend Snippet: Generation of hsd11b2 mutant zebrafish line. ( A ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. ( B ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. ( C ) Representative picture of hsd11b2 +/+ , hsd11b2 +/− and hsd11b2 −/− genotyping in 2% agarose gel. ( D ) RT-qPCR of hsd11b2 transcripts in hsd11b2 +/+ and hsd11b2 −/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t -test. * p

    Techniques Used: Mutagenesis, Sequencing, Genotyping Assay, Agarose Gel Electrophoresis, Quantitative RT-PCR

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    Solis BioDyne rt cdna synthesis mix
    Generation of hsd11b2 mutant zebrafish line. ( A ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. ( B ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. ( C ) Representative picture of hsd11b2 +/+ , hsd11b2 +/− and hsd11b2 −/− genotyping in 2% agarose gel. ( D ) <t>RT-qPCR</t> of hsd11b2 transcripts in hsd11b2 +/+ and hsd11b2 −/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t -test. * p
    Rt Cdna Synthesis Mix, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt cdna synthesis mix/product/Solis BioDyne
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt cdna synthesis mix - by Bioz Stars, 2022-07
    96/100 stars
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    Generation of hsd11b2 mutant zebrafish line. ( A ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. ( B ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. ( C ) Representative picture of hsd11b2 +/+ , hsd11b2 +/− and hsd11b2 −/− genotyping in 2% agarose gel. ( D ) RT-qPCR of hsd11b2 transcripts in hsd11b2 +/+ and hsd11b2 −/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t -test. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Knockout of the hsd11b2 Gene Extends the Cortisol Stress Response in Both Zebrafish Larvae and Adults

    doi: 10.3390/ijms222212525

    Figure Lengend Snippet: Generation of hsd11b2 mutant zebrafish line. ( A ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. ( B ) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. ( C ) Representative picture of hsd11b2 +/+ , hsd11b2 +/− and hsd11b2 −/− genotyping in 2% agarose gel. ( D ) RT-qPCR of hsd11b2 transcripts in hsd11b2 +/+ and hsd11b2 −/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t -test. * p

    Article Snippet: After treatment with RQ1 RNase-free DNase (Promega, M6101, Madison, WI, USA) to eliminate DNA genomic contamination, total RNA was retrotranscribed with FIREscript RT cDNA synthesis mix (Solis Biodyne, 06-13-0000S) according to the manufacturer’s protocol.

    Techniques: Mutagenesis, Sequencing, Genotyping Assay, Agarose Gel Electrophoresis, Quantitative RT-PCR