firescript rt cdna synthesis kit  (Solis BioDyne)


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    Solis BioDyne firescript rt cdna synthesis kit
    Expression of B. tabaci Asia II 1 putative genes in response to ChiLCV infection in RNA-Seq and <t>RT-qPCR.</t> The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values.
    Firescript Rt Cdna Synthesis Kit, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firescript rt cdna synthesis kit/product/Solis BioDyne
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    firescript rt cdna synthesis kit - by Bioz Stars, 2022-12
    96/100 stars

    Images

    1) Product Images from "Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector"

    Article Title: Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.890807

    Expression of B. tabaci Asia II 1 putative genes in response to ChiLCV infection in RNA-Seq and RT-qPCR. The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values.
    Figure Legend Snippet: Expression of B. tabaci Asia II 1 putative genes in response to ChiLCV infection in RNA-Seq and RT-qPCR. The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values.

    Techniques Used: Expressing, Infection, RNA Sequencing Assay, Quantitative RT-PCR

    2) Product Images from "RNA-protein interactome at the Hepatitis E virus internal ribosome entry site"

    Article Title: RNA-protein interactome at the Hepatitis E virus internal ribosome entry site

    Journal: bioRxiv

    doi: 10.1101/2022.04.11.487827

    Identification of HEV IRESl RNA-binding proteins by RaPID and Y3H assay. (A) Venny analysis of the HEV IRESl RNA-binding proteins identified by the RaPID assay. (B) Summary of the HEV IRESl RNA-binding proteins identified by the RaPID and the Y3H assay. (C) Y3H assay mediated confirmation of the interaction between HEV IRESl RNA and the host proteins identified by screening of the human liver cDNA library. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
    Figure Legend Snippet: Identification of HEV IRESl RNA-binding proteins by RaPID and Y3H assay. (A) Venny analysis of the HEV IRESl RNA-binding proteins identified by the RaPID assay. (B) Summary of the HEV IRESl RNA-binding proteins identified by the RaPID and the Y3H assay. (C) Y3H assay mediated confirmation of the interaction between HEV IRESl RNA and the host proteins identified by screening of the human liver cDNA library. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).

    Techniques Used: RNA Binding Assay, cDNA Library Assay, Transformation Assay

    Assessment of the ability of the HEV IRESl-binding host proteins (identified by the human liver cDNA library screening) to interact with the HCV IRES and the FMDV IRES. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
    Figure Legend Snippet: Assessment of the ability of the HEV IRESl-binding host proteins (identified by the human liver cDNA library screening) to interact with the HCV IRES and the FMDV IRES. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).

    Techniques Used: Binding Assay, cDNA Library Assay, Transformation Assay

    3) Product Images from "RNA-protein interactome at the Hepatitis E virus internal ribosome entry site"

    Article Title: RNA-protein interactome at the Hepatitis E virus internal ribosome entry site

    Journal: bioRxiv

    doi: 10.1101/2022.04.11.487827

    Identification of HEV IRESl RNA-binding proteins by RaPID and Y3H assay. (A) Venny analysis of the HEV IRESl RNA-binding proteins identified by the RaPID assay. (B) Summary of the HEV IRESl RNA-binding proteins identified by the RaPID and the Y3H assay. (C) Y3H assay mediated confirmation of the interaction between HEV IRESl RNA and the host proteins identified by screening of the human liver cDNA library. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
    Figure Legend Snippet: Identification of HEV IRESl RNA-binding proteins by RaPID and Y3H assay. (A) Venny analysis of the HEV IRESl RNA-binding proteins identified by the RaPID assay. (B) Summary of the HEV IRESl RNA-binding proteins identified by the RaPID and the Y3H assay. (C) Y3H assay mediated confirmation of the interaction between HEV IRESl RNA and the host proteins identified by screening of the human liver cDNA library. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).

    Techniques Used: RNA Binding Assay, cDNA Library Assay, Transformation Assay

    Assessment of the ability of the HEV IRESl-binding host proteins (identified by the human liver cDNA library screening) to interact with the HCV IRES and the FMDV IRES. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
    Figure Legend Snippet: Assessment of the ability of the HEV IRESl-binding host proteins (identified by the human liver cDNA library screening) to interact with the HCV IRES and the FMDV IRES. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).

    Techniques Used: Binding Assay, cDNA Library Assay, Transformation Assay

    4) Product Images from "Groundnut Bud Necrosis Virus Modulates the Expression of Innate Immune, Endocytosis, and Cuticle Development-Associated Genes to Circulate and Propagate in Its Vector, Thrips palmi"

    Article Title: Groundnut Bud Necrosis Virus Modulates the Expression of Innate Immune, Endocytosis, and Cuticle Development-Associated Genes to Circulate and Propagate in Its Vector, Thrips palmi

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.773238

    Expression of Thrips palmi putative genes in response to GBNV infection in RNA-Seq and reverse transcriptase-quantitative real-time PCR (RT-qPCR). The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values. Pearson’s correlation coefficient value was 0.81 as calculated using the CORREL function in MS Excel.
    Figure Legend Snippet: Expression of Thrips palmi putative genes in response to GBNV infection in RNA-Seq and reverse transcriptase-quantitative real-time PCR (RT-qPCR). The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values. Pearson’s correlation coefficient value was 0.81 as calculated using the CORREL function in MS Excel.

    Techniques Used: Expressing, Infection, RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    5) Product Images from "Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1"

    Article Title: Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

    Journal: Cells

    doi: 10.3390/cells11050833

    Stability and persistent efficacy of hsp70 dsRNA. ( a ) Detection of dsRNA in RT-PCR. M: 100 bp plus DNA ladder, L1–2: untreated control, RT-PCR amplicons of dsRNA in leaf tissue 1 h (L3–4), 3 h (L5–6), 6 h (L7–8), 24 h (L9–10), and 48 h (L11–12) post-application. ( b ) Mean percent mortality of adult B. tabaci exposed to hsp70 dsRNA-treated chilli plants. A total of 30 B. tabaci adults per plant were released at 24 h intervals, and mean percent mortality was calculated by normalizing the mortality in the control set. Values below each dot and bars on the dots indicate mean percent mortality of three replications and standard error of the mean (SEM), respectively. Each replicate contained five plants. Red arrows indicate the dates of spraying.
    Figure Legend Snippet: Stability and persistent efficacy of hsp70 dsRNA. ( a ) Detection of dsRNA in RT-PCR. M: 100 bp plus DNA ladder, L1–2: untreated control, RT-PCR amplicons of dsRNA in leaf tissue 1 h (L3–4), 3 h (L5–6), 6 h (L7–8), 24 h (L9–10), and 48 h (L11–12) post-application. ( b ) Mean percent mortality of adult B. tabaci exposed to hsp70 dsRNA-treated chilli plants. A total of 30 B. tabaci adults per plant were released at 24 h intervals, and mean percent mortality was calculated by normalizing the mortality in the control set. Values below each dot and bars on the dots indicate mean percent mortality of three replications and standard error of the mean (SEM), respectively. Each replicate contained five plants. Red arrows indicate the dates of spraying.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Designing and synthesis of dsRNA targeting Bemisia tabaci hsp70 and fas2 mRNA. ( a ) A conserved region of 128 (2416 to 2543) nt of B. tabaci hsp70 was selected for designing dsRNA. The putative siRNA is marked within the red box. ( b ) A conserved region of 150 (1085 to 1234) nt of B. tabaci fas2 was selected for designing dsRNA. The putative siRNA is marked within the red box. ( c ) Total RNA isolated from recombinant E. coli HT115 cells (L1–2); hsp70 dsRNA purified from total RNA using DNase I and RNase A (L3–6); and fas2 dsRNA purified from total RNA using DNase I and RNase A (L7–9) on 2% agarose gel stained with GoodView, M = 100 bp plus ladder.
    Figure Legend Snippet: Designing and synthesis of dsRNA targeting Bemisia tabaci hsp70 and fas2 mRNA. ( a ) A conserved region of 128 (2416 to 2543) nt of B. tabaci hsp70 was selected for designing dsRNA. The putative siRNA is marked within the red box. ( b ) A conserved region of 150 (1085 to 1234) nt of B. tabaci fas2 was selected for designing dsRNA. The putative siRNA is marked within the red box. ( c ) Total RNA isolated from recombinant E. coli HT115 cells (L1–2); hsp70 dsRNA purified from total RNA using DNase I and RNase A (L3–6); and fas2 dsRNA purified from total RNA using DNase I and RNase A (L7–9) on 2% agarose gel stained with GoodView, M = 100 bp plus ladder.

    Techniques Used: Isolation, Recombinant, Purification, Agarose Gel Electrophoresis, Staining

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    • firescript rt cdna synthesis kit  (Solis BioDyne)
    96
    Solis BioDyne firescript rt cdna synthesis kit
    Firescript Rt Cdna Synthesis Kit, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firescript rt cdna synthesis kit/product/Solis BioDyne
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    firescript rt cdna synthesis kit - by Bioz Stars, 2022-12
    96/100 stars
    		  Buy from Supplier

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