rt cdna synthesis kit (Solis BioDyne)
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Structured Review
Rt Cdna Synthesis Kit, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The Antimicrobial Activity of Pechuel-Loeschea leubnitziae Leaf Extract and its Effect on The Expression Level of Methicillin Resistant Staphylococcus Aureus and Candida albicans Virulence-Associated Genes"
Article Title: The Antimicrobial Activity of Pechuel-Loeschea leubnitziae Leaf Extract and its Effect on The Expression Level of Methicillin Resistant Staphylococcus Aureus and Candida albicans Virulence-Associated Genes
Journal: bioRxiv
doi: 10.1101/2022.01.11.475881
Figure Legend Snippet: Statistical variation of the mean CT values of 18S rRNA in control, EtOH-E treatment, and MeOH-E treatment from RT-qPCR. Key: ns-not significant, error bars-mean of triplicates ±SD (n=3), EtOH-E (ethanol extract), and MeOH-E (methanol extract) .
Techniques Used: Quantitative RT-PCR
Figure Legend Snippet: Statistical variation of the mean CT values of 16S rRNA in control, EtOH-E treatment, and MeOH-E treatment from RT-qPCR. Key: ns-not significant, error bars-mean of triplicates ±SD (n=3), EtOH-E (ethanol extract), and MeOH-E (methanol extract) .
Techniques Used: Quantitative RT-PCR
2) Product Images from "Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells"
Article Title: Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells
Journal: Heliyon
doi: 10.1016/j.heliyon.2021.e06041
Figure Legend Snippet: BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by cDNA synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P
Techniques Used: Expressing, Real-time Polymerase Chain Reaction
3) Product Images from "The Enzymatic Core of the Parkinson’s Disease-Associated Protein LRRK2 Impairs Mitochondrial Biogenesis in Aging Yeast"
Article Title: The Enzymatic Core of the Parkinson’s Disease-Associated Protein LRRK2 Impairs Mitochondrial Biogenesis in Aging Yeast
Journal: Frontiers in Molecular Neuroscience
doi: 10.3389/fnmol.2018.00205
Figure Legend Snippet: LRRK2 RCK compromises mitochondrial biogenesis and complex IV assembly. (A) Flow cytometric quantification of wild type and ATG11 deletion strains harboring Om45-GFP and expressing LacZ, LRRK2 RCK or R1398L RCK on day 1 and 2 of aging. Dead cells were excluded via PI counterstaining. Values were normalized to control cells on day 1. Means ± SEM; n = 4. (B) Flow cytometric quantification of PI-stained wild type and ATG1 , ATG11 and ATG32 deletion strains expressing LacZ or LRRK2 RCK on day 3 of aging. Means ± SEM; n = 4. (C) q-RT-PCR to determine mRNA levels of indicated mitochondria-related transcripts in cells expressing LacZ, LRRK2 RCK or R1398L RCK . Normalization was performed using mRNA levels of UBC6 . Means ± SEM; n = 4. (D) Immunoblot analysis of mitochondria isolated from cells expressing LacZ or LRRK2 RCK on day 1. Blots were probed with antibodies directed against the respiratory chain complex IV components Cox1, Cox2, Cox4 and Cox6, as well as Sdh1 (subunit of complex II), Tom70 (outer mitochondrial membrane) and Tim44 (inner mitochondrial membrane). (E) Immunoblots of Blue-Native-PAGE with samples described in (D) . Blots were probed with antibodies against Cox6 (complex III/IV), Atp14 (complex V) and Tom22 (translocase of outer membrane (TOM) complex). (F) In-gel ATPase activity assay of samples described in (D) . (G) Measurement of mitochondrial transmembrane potential (ΔΨ m ) in mitochondria isolated from cells described in (D) . Values for ΔΨ m were normalized to mitochondria from control cells. Means ± SEM; n = 3. *** p
Techniques Used: Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Isolation, Western Blot, Blue Native PAGE, Activity Assay
4) Product Images from "Groundnut Bud Necrosis Virus Modulates the Expression of Innate Immune, Endocytosis, and Cuticle Development-Associated Genes to Circulate and Propagate in Its Vector, Thrips palmi"
Article Title: Groundnut Bud Necrosis Virus Modulates the Expression of Innate Immune, Endocytosis, and Cuticle Development-Associated Genes to Circulate and Propagate in Its Vector, Thrips palmi
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2022.773238
Figure Legend Snippet: Expression of Thrips palmi putative genes in response to GBNV infection in RNA-Seq and reverse transcriptase-quantitative real-time PCR (RT-qPCR). The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values. Pearson’s correlation coefficient value was 0.81 as calculated using the CORREL function in MS Excel.
Techniques Used: Expressing, Infection, RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR
5) Product Images from "Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector"
Article Title: Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2022.890807
Figure Legend Snippet: Expression of B. tabaci Asia II 1 putative genes in response to ChiLCV infection in RNA-Seq and RT-qPCR. The values of log 2 -fold changes calculated in RNA-Seq analysis were in accordance with the RT-qPCR fold change values.
Techniques Used: Expressing, Infection, RNA Sequencing Assay, Quantitative RT-PCR
6) Product Images from "RNA-protein interactome at the Hepatitis E virus internal ribosome entry site"
Article Title: RNA-protein interactome at the Hepatitis E virus internal ribosome entry site
Journal: bioRxiv
doi: 10.1101/2022.04.11.487827
Figure Legend Snippet: Identification of HEV IRESl RNA-binding proteins by RaPID and Y3H assay. (A) Venny analysis of the HEV IRESl RNA-binding proteins identified by the RaPID assay. (B) Summary of the HEV IRESl RNA-binding proteins identified by the RaPID and the Y3H assay. (C) Y3H assay mediated confirmation of the interaction between HEV IRESl RNA and the host proteins identified by screening of the human liver cDNA library. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
Techniques Used: RNA Binding Assay, cDNA Library Assay, Transformation Assay
Figure Legend Snippet: Assessment of the ability of the HEV IRESl-binding host proteins (identified by the human liver cDNA library screening) to interact with the HCV IRES and the FMDV IRES. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
Techniques Used: Binding Assay, cDNA Library Assay, Transformation Assay
7) Product Images from "RNA-protein interactome at the Hepatitis E virus internal ribosome entry site"
Article Title: RNA-protein interactome at the Hepatitis E virus internal ribosome entry site
Journal: bioRxiv
doi: 10.1101/2022.04.11.487827
Figure Legend Snippet: Identification of HEV IRESl RNA-binding proteins by RaPID and Y3H assay. (A) Venny analysis of the HEV IRESl RNA-binding proteins identified by the RaPID assay. (B) Summary of the HEV IRESl RNA-binding proteins identified by the RaPID and the Y3H assay. (C) Y3H assay mediated confirmation of the interaction between HEV IRESl RNA and the host proteins identified by screening of the human liver cDNA library. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
Techniques Used: RNA Binding Assay, cDNA Library Assay, Transformation Assay
Figure Legend Snippet: Assessment of the ability of the HEV IRESl-binding host proteins (identified by the human liver cDNA library screening) to interact with the HCV IRES and the FMDV IRES. YBZ1 strain was transformed in the indicated combinations and plated on media lacking Leucine and Uracil (LU - ). Four random colonies from each cotransformant plate were replica plated onto media lacking Leucine, Uracil, Histidine (LUH - ) and supplemented with 5mM or 10mM 3-amino 1, 2, 4 Triazole (3-AT). Same colonies were used in liquid β-galactosidase assay. Relative β-galactosidase units are plotted as mean (± SEM).
Techniques Used: Binding Assay, cDNA Library Assay, Transformation Assay
8) Product Images from "First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA"
Article Title: First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA
Journal: Insects
doi: 10.3390/insects11110769
Figure Legend Snippet: RT-PCR results showing presence of dsRNA (dsGFP applied at both 2.5 and 5 µg/µL) on bud tissue at 1 h, and 1, 2 and 3 d post dsRNA-application.
Techniques Used: Reverse Transcription Polymerase Chain Reaction
9) Product Images from "Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1"
Article Title: Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1
Journal: Cells
doi: 10.3390/cells11050833
Figure Legend Snippet: Stability and persistent efficacy of hsp70 dsRNA. ( a ) Detection of dsRNA in RT-PCR. M: 100 bp plus DNA ladder, L1–2: untreated control, RT-PCR amplicons of dsRNA in leaf tissue 1 h (L3–4), 3 h (L5–6), 6 h (L7–8), 24 h (L9–10), and 48 h (L11–12) post-application. ( b ) Mean percent mortality of adult B. tabaci exposed to hsp70 dsRNA-treated chilli plants. A total of 30 B. tabaci adults per plant were released at 24 h intervals, and mean percent mortality was calculated by normalizing the mortality in the control set. Values below each dot and bars on the dots indicate mean percent mortality of three replications and standard error of the mean (SEM), respectively. Each replicate contained five plants. Red arrows indicate the dates of spraying.
Techniques Used: Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: Designing and synthesis of dsRNA targeting Bemisia tabaci hsp70 and fas2 mRNA. ( a ) A conserved region of 128 (2416 to 2543) nt of B. tabaci hsp70 was selected for designing dsRNA. The putative siRNA is marked within the red box. ( b ) A conserved region of 150 (1085 to 1234) nt of B. tabaci fas2 was selected for designing dsRNA. The putative siRNA is marked within the red box. ( c ) Total RNA isolated from recombinant E. coli HT115 cells (L1–2); hsp70 dsRNA purified from total RNA using DNase I and RNase A (L3–6); and fas2 dsRNA purified from total RNA using DNase I and RNase A (L7–9) on 2% agarose gel stained with GoodView, M = 100 bp plus ladder.
Techniques Used: Isolation, Recombinant, Purification, Agarose Gel Electrophoresis, Staining
10) Product Images from "Expression of Oncolytic Adenovirus-Encoded RNAi Molecules Is Most Effective in a pri-miRNA Precursor Format"
Article Title: Expression of Oncolytic Adenovirus-Encoded RNAi Molecules Is Most Effective in a pri-miRNA Precursor Format
Journal: Molecular Therapy Oncolytics
doi: 10.1016/j.omto.2020.10.012
Figure Legend Snippet: Analysis of Oncolytic Adenoviruses Expressing Different MicroRNA Precursor Molecules (A) Dose-response cytotoxicity analysis of oncolytic adenoviruses expressing different miR-1 precursor molecules on A549 lung cancer cells. Data shown are means of two independent experiments in triplicate. (B) Quantitative RT-PCR analysis of mature miR-1-3p expression in HCT116 parental and Drosha knockout (KO) colorectal carcinoma cells infected with oncolytic adenoviruses expressing different miR-1 precursor molecules. (C) Expression of mature miR-1-3p in different cancer cell lines infected with AdΔ24E3-U6 or AdΔ24E3-U6.pri-miR-1. (D) Time course analysis of mature miR-1-3p expression in A549 cells infected with oncolytic adenoviruses expressing different miR-1 precursor molecules. (E) Comparison of mature miR-1-3p expression in HCT116 cells infected with AdΔ24E3-U6 or AdΔ24E3-U6.pri-miR-1, before and after 6 weeks of virus propagation. Data shown in (B)–(E) are means of three independent experiments with cells infected at 100 IU/cell in duplicate and are given relative to uninfected cells.
Techniques Used: Expressing, Quantitative RT-PCR, Knock-Out, Infection