Journal: PLoS ONE
Article Title: Detection of treponemes in digital dermatitis lesions of captive European bison (Bison bonasus)
Figure Lengend Snippet: Histological appearance of interdigital skin of European bison ( Bison bonasus ) with digital dermatitis. Left hind foot of animal no. 7. A: The dermatitis is characterized by epithelial acanthosis, ballooning degeneration of keratinocytes and elongated, dermal papillae containing mixed cellular infiltrate (arrows) protruding to the surface. Large amounts of basophilic bacteria are infiltrating the superficial epidermis (arrowhead). H E, bar 100 μm. Rectangle shows selected area for in situ hybridization on a parallel section. B: Bacteria visualized as bright green organisms (*) infiltrating the superficial epidermis. Fluorescent in situ hybridization. Bar 25 μm. C: Treponema organisms (arrows) infiltrating deep into the epidermis whereas other bacteria (arrowheads) are seen superficially. Double fluorescent in situ hybridization with probe for genus Treponema (Cy3 labelled) and for domain Bacterium (fluorescein labelled). Bar 25 μm.
Article Snippet: Specific nested PCR assays for T . medium , T . phagedenis , and T . pedis , were performed on swab and pooled biopsy samples according to Evans et al. [ ] using FIREPol® Master Mix Ready to load with 12.5 mM MgCl2 (Solis Biodyne, Tartu, Estonia).
Techniques: In Situ Hybridization