firepol  (Solis BioDyne)


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    Name:
    FIREPol DNA Polymerase
    Description:
    Genetically modified thermostable Taq DNA Polymerase that provides robust and reproducible results
    Catalog Number:
    01-01-00500
    Price:
    None
    Category:
    Endpoint PCR
    Applications:
    PCR, sanger sequencing, cloning
    Size:
    500 U | 100 µl
    Buy from Supplier


    Structured Review

    Solis BioDyne firepol
    FIREPol DNA Polymerase
    Genetically modified thermostable Taq DNA Polymerase that provides robust and reproducible results
    https://www.bioz.com/result/firepol/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    firepol - by Bioz Stars, 2021-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea
    Article Snippet: .. Polymerase chain reaction (PCR) was performed in a 20 μl reaction mixture containing 0.1 μl FIREPol DNA Polymerase (0.5 units) (Solisbiodyne, Tartu, Estonia), 1 μl each primer (10 μM), 0.2 μl dNTP mix (200 μM), 2 μl 10× buffer, 1 μl template (approximately 100 ng), 4 μl 5× Q-solution, and 10.7 μl sterilized dH2 O. Amplifications were performed with an initial denaturation step at 94°C for 3 min followed by 35 cycles of 15 s at 94°C, 15 s at 53°C and 60 s at 72°C for ITS region, 35 cycles of 15 s at 94°C, 15 s at 57°C and 60 s at 72°C for GAPDH , 35 cycles of 15 s at 94°C, 15 s at 56°C and 2 min at 72°C for RPB2 , 35 cycles of 15 s at 94°C, 15 s at 67°C and 60 s at 72°C for HIS3, and final extension at 72°C for 7 min. Amplified fragments were loaded on an agarose gel (0.8% w/v or 1.5% w/v) stained with RedSafe Nucleic Acid Staining Solution (20,000×) (Intronbio, Seongnam, Korea) and visualized under UV light. ..

    Article Title: Frequency of beta S globin gene haplotypes among sickle cell patients in Nigeria
    Article Snippet: .. Subsequently, SCD polymorphisms were investigated using polymerase chain reaction (PCR) amplification using commercially available PCR kit FIREPol DNA polymerase (Solis BioDyne Inc, Tartu, Estonia) with seven different primers Xmn1 (650 base pair [bp]), HindIIIG (780 bp), HindIIIA (760 bp), HincIIΨβ (701 bp), HincIIa (590 bp), HinfI (380 bp) and HpaI (620 bp) (Creative Biogene, Shirley, NY, USA; Jena Bioscience, Jena, Germany). ..

    Article Title: Basic β-1,3-Glucanase from Drosera binata Exhibits Antifungal Potential in Transgenic Tobacco Plants
    Article Snippet: .. The PCR reaction mixture of 50 µL contained 200 ng DNA template, 20 pmol of each primer, 0.2 mM dNTPs, 1 × PCR buffer, 2.5 mM MgCl2, and 2U FirePol DNA polymerase (Solis BioDyne, Tartu, Estonia). ..

    Article Title: Securing genetic integrity in freshwater pearl mussel propagation and captive breeding
    Article Snippet: .. Polymerase chain reactions (PCRs) were performed in a total volume of 12.5 µL containing 25 ng genomic DNA, 0.2 µM of each primer, 0.2 mM of each dNTP, 3 mM MgCl2 for eight Loci (2 mM MgCl2 for Locus 5280), 1 × FirePol® PCR buffer BD (0.8 M Tris–HCl, 0.2 M (NH4 )SO4 and 0.5 U FirePol® Taq DNA polymerase (Solis Biodyne, Tartu, Estonia) under the cycling conditions described in Geist et al. and Geist & Kuehn . ..

    Article Title: Global analysis of putative phospholipases in the malaria parasite Plasmodium falciparum reveals critical factors for parasite proliferation
    Article Snippet: .. Successful integration was confirmed by diagnostic PCR using FIREpol DNA polymerase (Solis BioDyne). ..

    Article Title: Basic β-1,3-Glucanase from Drosera binata Exhibits Antifungal Potential in Transgenic Tobacco Plants
    Article Snippet: .. The 25 µL PCR reaction mixture contained 100–200 ng DNA template, 10 pmol of each primer, 0.2 mM dNTPs, PCR buffer, 2.5 mM MgCl2 , and 1 U FIREPol Taq DNA polymerase (Solis BioDyne, Tartu, Estonia). ..

    Amplification:

    Article Title: Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea
    Article Snippet: .. Polymerase chain reaction (PCR) was performed in a 20 μl reaction mixture containing 0.1 μl FIREPol DNA Polymerase (0.5 units) (Solisbiodyne, Tartu, Estonia), 1 μl each primer (10 μM), 0.2 μl dNTP mix (200 μM), 2 μl 10× buffer, 1 μl template (approximately 100 ng), 4 μl 5× Q-solution, and 10.7 μl sterilized dH2 O. Amplifications were performed with an initial denaturation step at 94°C for 3 min followed by 35 cycles of 15 s at 94°C, 15 s at 53°C and 60 s at 72°C for ITS region, 35 cycles of 15 s at 94°C, 15 s at 57°C and 60 s at 72°C for GAPDH , 35 cycles of 15 s at 94°C, 15 s at 56°C and 2 min at 72°C for RPB2 , 35 cycles of 15 s at 94°C, 15 s at 67°C and 60 s at 72°C for HIS3, and final extension at 72°C for 7 min. Amplified fragments were loaded on an agarose gel (0.8% w/v or 1.5% w/v) stained with RedSafe Nucleic Acid Staining Solution (20,000×) (Intronbio, Seongnam, Korea) and visualized under UV light. ..

    Article Title: Frequency of beta S globin gene haplotypes among sickle cell patients in Nigeria
    Article Snippet: .. Subsequently, SCD polymorphisms were investigated using polymerase chain reaction (PCR) amplification using commercially available PCR kit FIREPol DNA polymerase (Solis BioDyne Inc, Tartu, Estonia) with seven different primers Xmn1 (650 base pair [bp]), HindIIIG (780 bp), HindIIIA (760 bp), HincIIΨβ (701 bp), HincIIa (590 bp), HinfI (380 bp) and HpaI (620 bp) (Creative Biogene, Shirley, NY, USA; Jena Bioscience, Jena, Germany). ..

    Agarose Gel Electrophoresis:

    Article Title: Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea
    Article Snippet: .. Polymerase chain reaction (PCR) was performed in a 20 μl reaction mixture containing 0.1 μl FIREPol DNA Polymerase (0.5 units) (Solisbiodyne, Tartu, Estonia), 1 μl each primer (10 μM), 0.2 μl dNTP mix (200 μM), 2 μl 10× buffer, 1 μl template (approximately 100 ng), 4 μl 5× Q-solution, and 10.7 μl sterilized dH2 O. Amplifications were performed with an initial denaturation step at 94°C for 3 min followed by 35 cycles of 15 s at 94°C, 15 s at 53°C and 60 s at 72°C for ITS region, 35 cycles of 15 s at 94°C, 15 s at 57°C and 60 s at 72°C for GAPDH , 35 cycles of 15 s at 94°C, 15 s at 56°C and 2 min at 72°C for RPB2 , 35 cycles of 15 s at 94°C, 15 s at 67°C and 60 s at 72°C for HIS3, and final extension at 72°C for 7 min. Amplified fragments were loaded on an agarose gel (0.8% w/v or 1.5% w/v) stained with RedSafe Nucleic Acid Staining Solution (20,000×) (Intronbio, Seongnam, Korea) and visualized under UV light. ..

    Staining:

    Article Title: Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea
    Article Snippet: .. Polymerase chain reaction (PCR) was performed in a 20 μl reaction mixture containing 0.1 μl FIREPol DNA Polymerase (0.5 units) (Solisbiodyne, Tartu, Estonia), 1 μl each primer (10 μM), 0.2 μl dNTP mix (200 μM), 2 μl 10× buffer, 1 μl template (approximately 100 ng), 4 μl 5× Q-solution, and 10.7 μl sterilized dH2 O. Amplifications were performed with an initial denaturation step at 94°C for 3 min followed by 35 cycles of 15 s at 94°C, 15 s at 53°C and 60 s at 72°C for ITS region, 35 cycles of 15 s at 94°C, 15 s at 57°C and 60 s at 72°C for GAPDH , 35 cycles of 15 s at 94°C, 15 s at 56°C and 2 min at 72°C for RPB2 , 35 cycles of 15 s at 94°C, 15 s at 67°C and 60 s at 72°C for HIS3, and final extension at 72°C for 7 min. Amplified fragments were loaded on an agarose gel (0.8% w/v or 1.5% w/v) stained with RedSafe Nucleic Acid Staining Solution (20,000×) (Intronbio, Seongnam, Korea) and visualized under UV light. ..

    Diagnostic Assay:

    Article Title: Global analysis of putative phospholipases in the malaria parasite Plasmodium falciparum reveals critical factors for parasite proliferation
    Article Snippet: .. Successful integration was confirmed by diagnostic PCR using FIREpol DNA polymerase (Solis BioDyne). ..

    Plasmid Preparation:

    Article Title: Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domains
    Article Snippet: .. Plasmid mixes and bacterial colonies were tested for possible contamination at various steps using FIREPol DNA Polymerase (Solis BioDyne): antitoxins were tested with the combination of pK223_rev_CPEC and STEC_panA_ctrl2, VH_panA_ctrl1 or Bsup_panA_ctrl1 primers, and toxins with the combination of pBAD_fwd and STEC_TOX_ctrl1, VH_TOX_ctrl1, Bsup_TOX_ctrl1 ( Table S2). ..

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  • 99
    Solis BioDyne fire pol dna polymerase
    Results of 27 <t>LR-PCR</t> amplifications for one patient. Line 1 - ladder λ <t>DNA</t> Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.
    Fire Pol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fire pol dna polymerase/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fire pol dna polymerase - by Bioz Stars, 2021-10
    99/100 stars
      Buy from Supplier

    99
    Solis BioDyne hot firepol dna polymerase
    Results of 27 <t>LR-PCR</t> amplifications for one patient. Line 1 - ladder λ <t>DNA</t> Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.
    Hot Firepol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol dna polymerase/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot firepol dna polymerase - by Bioz Stars, 2021-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Results of 27 LR-PCR amplifications for one patient. Line 1 - ladder λ DNA Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.

    Journal: Scientific Reports

    Article Title: Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia

    doi: 10.1038/s41598-017-15657-2

    Figure Lengend Snippet: Results of 27 LR-PCR amplifications for one patient. Line 1 - ladder λ DNA Hind III; 2 - GABRA1 (17533 bp), 3 - ABCB1 (16809 bp); 4 - ABCB1 (17000 bp); 5 - NQO1 (11767 bp); 6 - ABCB1 (14483 bp); 7- ABCB1 (15827 bp); 8 - GABRA1 (10134 bp); 9 - ALB (11245 bp); 10 - CYP2C9 (17670 bp); 11 - CYP2C9 (10723 bp); 12 – ladder 100 bp; 13 - UGT1A9 (972 bp); 14 - ADRA1A (1183 bp); 15 - UGT1A9 (2303 bp); 16 - SULT1A1 (2760 bp); 17 - CYP2C9 (2761 bp); 18 - CYP2B6 (3312 bp); 19 - SULT1A1 (3219 bp); 20 - ABCB1 (3518 bp); 21 - CYP2B6 (3113 bp); 22 - ADRA1A (6818 bp); 23 - CYP2B6 (9280 bp); 24 - GABRA1 (9190 bp); 25 - ABCB1 (5967 bp); 26 - ALB (8571 bp); 27 - NQO1 (8998 bp); 28 - UGT1A9 (6700 bp); 29 - SULT1A1 (6497 bp); 30 – ladder 1 kbp;. Lines 1–11, 0.5% agarose gel; lines 12–14, 1.5% agarose gel; lines 15–30, 1.0% agarose gel. The gel images were obtained by trimming and colour adjusting of the full-length gels in the IrfanView 4.44 program.

    Article Snippet: After optimization, LR-PCR reactions were carried out on an Applied Biosystems 2720 Thermal Cycler (Applied Biosystems, Foster City, CA) with the use of one of three polymerase sets: GoTaq® Long PCR Master Mix (Promega, Madison, USA), Long & High Fidelity PCR Enzyme Mix (BiotechRabbit, Hennigsdorf, Germany), Fire Pol® DNA Polymerase (Solis BioDyne, Tartu, Estonia) on the total volume of 30 μL, according to the manufacturer’s instructions (Table ).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis