forskolin  (Alomone Labs)


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  • 94
    Name:
    Forskolin
    Description:
    Forskolin increases cAMP levels by activating adenylate cyclase
    Catalog Number:
    F-500
    Price:
    55.0
    Category:
    Small Molecule
    Source:
    Coleus forskohlii.
    Applications:
    0
    Purity:
    >97% (HPLC)
    Size:
    5 mg
    Format:
    Lyophilized/solid.
    Formula:
    C22H34O7
    Molecular Weight:
    410.5
    Molecule Name:
    [3R-(3a,4ab,5b,6b,6aa,10a,10ab,10ba)]-5-(Acetyloxy)-3-e thenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pent amethyl-1H-naphtho[2,1-b]pyran-1-one.
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    Structured Review

    Alomone Labs forskolin
    Forskolin
    Forskolin increases cAMP levels by activating adenylate cyclase
    https://www.bioz.com/result/forskolin/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    forskolin - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Quantitative profiling of axonal guidance proteins during the differentiation of human neurospheres"

    Article Title: Quantitative profiling of axonal guidance proteins during the differentiation of human neurospheres

    Journal: bioRxiv

    doi: 10.1101/2021.02.21.432144

    Neurite outgrowth is regulated by cAMP signaling. A. Plated neurospheres were treated with 10 μM forskolin for 24 h. The number of neurites crossing a circular mask around the neurosphere was used for quantification. B. Micrographs of control and 10 μM forskolin-treated neurospheres immunostained for beta III tubulin (green). Nuclei counterstained with DAPI (blue). C. Number of neurites crossing the circle (**p
    Figure Legend Snippet: Neurite outgrowth is regulated by cAMP signaling. A. Plated neurospheres were treated with 10 μM forskolin for 24 h. The number of neurites crossing a circular mask around the neurosphere was used for quantification. B. Micrographs of control and 10 μM forskolin-treated neurospheres immunostained for beta III tubulin (green). Nuclei counterstained with DAPI (blue). C. Number of neurites crossing the circle (**p

    Techniques Used:

    2) Product Images from "Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction"

    Article Title: Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0117350

    At low depolarisation K v 7 channel inhibition reduces the response to forskolin. Strips pre-constricted with 20 mM KPSS were treated with either 0.1 μM isoprenaline (A) or 1 μM forskolin (B). Pre-treatment with K v 7 channel modulators revealed a reduction in response to isoprenaline and forskolin when blocking K v 7 channels by XE991 (10 μM) whereas retigabine (10μM) did not affect the response to isoprenaline. Pre-treatment with vehicle control (0.1% DMSO). ** P
    Figure Legend Snippet: At low depolarisation K v 7 channel inhibition reduces the response to forskolin. Strips pre-constricted with 20 mM KPSS were treated with either 0.1 μM isoprenaline (A) or 1 μM forskolin (B). Pre-treatment with K v 7 channel modulators revealed a reduction in response to isoprenaline and forskolin when blocking K v 7 channels by XE991 (10 μM) whereas retigabine (10μM) did not affect the response to isoprenaline. Pre-treatment with vehicle control (0.1% DMSO). ** P

    Techniques Used: Inhibition, Blocking Assay

    Modulation of K v 7 channels does not affect Gs mediated relaxation at 1 μM carbachol-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 1 μM carbachol. * P
    Figure Legend Snippet: Modulation of K v 7 channels does not affect Gs mediated relaxation at 1 μM carbachol-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 1 μM carbachol. * P

    Techniques Used: Produced, Incubation

    Modulation of K v 7 channels does not affect Gs mediated relaxation at 40 mM KPSS-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 40 mM KPSS. Neither of the K v 7 channel modulators affected the response to isoprenaline or forskolin.
    Figure Legend Snippet: Modulation of K v 7 channels does not affect Gs mediated relaxation at 40 mM KPSS-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 40 mM KPSS. Neither of the K v 7 channel modulators affected the response to isoprenaline or forskolin.

    Techniques Used: Produced, Incubation

    Effect of isoprenaline and forskolin in human DSM at 1 μM carbachol- and 40 mM KPSS-induced contractions. Cumulative concentration-responses were induced with isoprenaline and forskolin. Initial tone was achieved by pre-constriction with 1 μM carbachol (A and B) and 40 mM KPSS (C and D) bladder strips. * P
    Figure Legend Snippet: Effect of isoprenaline and forskolin in human DSM at 1 μM carbachol- and 40 mM KPSS-induced contractions. Cumulative concentration-responses were induced with isoprenaline and forskolin. Initial tone was achieved by pre-constriction with 1 μM carbachol (A and B) and 40 mM KPSS (C and D) bladder strips. * P

    Techniques Used: Concentration Assay

    3) Product Images from "Regulation of basal expression of hepatic PEPCK and G6Pase by AKT2"

    Article Title: Regulation of basal expression of hepatic PEPCK and G6Pase by AKT2

    Journal: The Biochemical journal

    doi: 10.1042/BCJ20190570

    CREB phosphorylation mediates AKT2-regulated induction of PEPCK. ( A ) CREB phosphorylation induced by PTEN loss is regulated by AKT2. Liver lysates from mice with different genotypes were analysis for p-CREB. PM, Pten deleted; DM, Pten and Akt2 deleted; AM, Akt2 deleted. Two biological replicas are included for each genotype group. Protein levels of p-AKT, PTEN and p-GSK3 are analyzed and showed up-regulation of AKT and GSK-3 with PTEN loss and inhibition when AKT2 is lost. Phosphorylation of CREB is only observed when PTEN is lost. AKT2 loss together with PTEN blocked this phosphorylation in the DM livers. ( B ) Akt2 +/+ and Akt2 − / − cell were treated with forskolin (100 μM) for 24 h to induce cAMP and CREB phosphorylation or vehicle as controls. Phosphorylation of CREB is detected and showed to be lower when AKT2 is lost. When forskolin is added to induce cAMP, protein expression of p-CREB is evaluated in both Akt2 +/+ and Akt2 − / − cells even though its levels remains lower in the Akt2 − / − cells. Vinculin is probed as loading control. ( C ) Introduction of CREB-133A and wtCREB to Akt2 +/+ and Akt2 −/− hepatocytes. Expression of PEPCK is analyzed using quantitative PCR analysis. Left panel, CREB-S133A is introduced to Akt2 +/+ cells. Middle panel, wtCREB is introduced to Akt2 −/− cells; Right panel, wtCREB is introduced to Akt2 +/+ cells. n = 3. Data presented as mean ± SEM. * P ≤ 0.05. Different from vector transfected cells of the same genotype. Experiments repeated at least three times.
    Figure Legend Snippet: CREB phosphorylation mediates AKT2-regulated induction of PEPCK. ( A ) CREB phosphorylation induced by PTEN loss is regulated by AKT2. Liver lysates from mice with different genotypes were analysis for p-CREB. PM, Pten deleted; DM, Pten and Akt2 deleted; AM, Akt2 deleted. Two biological replicas are included for each genotype group. Protein levels of p-AKT, PTEN and p-GSK3 are analyzed and showed up-regulation of AKT and GSK-3 with PTEN loss and inhibition when AKT2 is lost. Phosphorylation of CREB is only observed when PTEN is lost. AKT2 loss together with PTEN blocked this phosphorylation in the DM livers. ( B ) Akt2 +/+ and Akt2 − / − cell were treated with forskolin (100 μM) for 24 h to induce cAMP and CREB phosphorylation or vehicle as controls. Phosphorylation of CREB is detected and showed to be lower when AKT2 is lost. When forskolin is added to induce cAMP, protein expression of p-CREB is evaluated in both Akt2 +/+ and Akt2 − / − cells even though its levels remains lower in the Akt2 − / − cells. Vinculin is probed as loading control. ( C ) Introduction of CREB-133A and wtCREB to Akt2 +/+ and Akt2 −/− hepatocytes. Expression of PEPCK is analyzed using quantitative PCR analysis. Left panel, CREB-S133A is introduced to Akt2 +/+ cells. Middle panel, wtCREB is introduced to Akt2 −/− cells; Right panel, wtCREB is introduced to Akt2 +/+ cells. n = 3. Data presented as mean ± SEM. * P ≤ 0.05. Different from vector transfected cells of the same genotype. Experiments repeated at least three times.

    Techniques Used: Mouse Assay, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Transfection

    4) Product Images from "Caveolin-1 and Lipid Microdomains Regulate Gs Trafficking and Attenuate Gs/Adenylyl Cyclase Signaling"

    Article Title: Caveolin-1 and Lipid Microdomains Regulate Gs Trafficking and Attenuate Gs/Adenylyl Cyclase Signaling

    Journal: Molecular Pharmacology

    doi: 10.1124/mol.109.060160

    Caveolin knockdown increases forskolin- and fluoride-stimulated Gα s /adenylyl cyclase signaling. A, wild-type C6 cells (WT C6) or caveolin-1 stable knockdown cells (CAV-1 RNAi) were incubated with [2,8- 3 H]adenine for 18 h to label cellular ATP.
    Figure Legend Snippet: Caveolin knockdown increases forskolin- and fluoride-stimulated Gα s /adenylyl cyclase signaling. A, wild-type C6 cells (WT C6) or caveolin-1 stable knockdown cells (CAV-1 RNAi) were incubated with [2,8- 3 H]adenine for 18 h to label cellular ATP.

    Techniques Used: Incubation

    Caveolin-1 knockout increases fluoride and forskolin-stimulated adenylyl cyclase activity in mouse striatum. Ten- to 12-week-old wild-type or caveolin-1 knockout littermate mice were colony bred, brains were obtained, and striatum was microdissected.
    Figure Legend Snippet: Caveolin-1 knockout increases fluoride and forskolin-stimulated adenylyl cyclase activity in mouse striatum. Ten- to 12-week-old wild-type or caveolin-1 knockout littermate mice were colony bred, brains were obtained, and striatum was microdissected.

    Techniques Used: Knock-Out, Activity Assay, Mouse Assay

    Related Articles

    other:

    Article Title: Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction
    Article Snippet: Organ bath studies Retigabine and forskolin were purchased from Alamone Labs (Jerusalem, Israel) whereas XE991 was kindly provided by NeuroSearch (Ballerup, Denmark), and ML213 and ML277 were kindly provided by Vanderbilt University (Nashville, TN, USA (MLCPN probes)).

    Article Title: CFTR channel in oocytes from Xenopus laevis and its regulation by xShroom1 protein.
    Article Snippet: Shroom is a family of related proteins linked to the actin cytoskeleton. xShroom1 is constitutively expressed in Xenopus laevis oocytes, and it is required for the expression of the epithelial sodium channel (ENaC).. As there is a close relationship between ENaC and the cystic fibrosis transmembrane regulator (CFTR), we examined the action of xShroom1 on CFTR expression and activity.

    Incubation:

    Article Title: Regulation of basal expression of hepatic PEPCK and G6Pase by AKT2
    Article Snippet: .. The medium was replaced with fresh medium with 100 μM Forskolin (Alomone Labs Cat# F-500) or vehicle and incubated for 24 h before harvesting. ..

    Mouse Assay:

    Article Title: Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation.
    Article Snippet: .. Peripheral nerve myelination involves rapid production of tightly bound lipid layers requiring cholesterol biosynthesis and myelin protein expression, but also a collagen-containing extracellular matrix providing mechanical stability.. In previous studies, we showed a function of ascorbic acid in peripheral nerve myelination and extracellular matrix formation in adult mice. ..

    Immunocytochemistry:

    Article Title: Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation.
    Article Snippet: .. Peripheral nerve myelination involves rapid production of tightly bound lipid layers requiring cholesterol biosynthesis and myelin protein expression, but also a collagen-containing extracellular matrix providing mechanical stability.. In previous studies, we showed a function of ascorbic acid in peripheral nerve myelination and extracellular matrix formation in adult mice. ..

    Western Blot:

    Article Title: Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation.
    Article Snippet: .. Peripheral nerve myelination involves rapid production of tightly bound lipid layers requiring cholesterol biosynthesis and myelin protein expression, but also a collagen-containing extracellular matrix providing mechanical stability.. In previous studies, we showed a function of ascorbic acid in peripheral nerve myelination and extracellular matrix formation in adult mice. ..

    Cell Culture:

    Article Title: Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation.
    Article Snippet: .. Peripheral nerve myelination involves rapid production of tightly bound lipid layers requiring cholesterol biosynthesis and myelin protein expression, but also a collagen-containing extracellular matrix providing mechanical stability.. In previous studies, we showed a function of ascorbic acid in peripheral nerve myelination and extracellular matrix formation in adult mice. ..

    Concentration Assay:

    Article Title: Feedback inhibition of cAMP effector signaling by a chaperone-assisted ubiquitin system
    Article Snippet: .. Forskolin (Alomone Labs, Jerusalem, Israel) was prepared as a stock solution (50 mM, in DMSO) and stored at −80ο C until used at final concentration of 50 µM in the bath aCSF. ..

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  • 80
    Alomone Labs anti α actin
    Kv7.1 and Kv7.5 expression in optimal cutting temperature compound (OCT)-embedded malignant vascular tumors. Consecutive sections of vascular tumor samples were stained by hematoxylin-eosin (HE) and immunofluorescent labeling for Kv7.1 and Kv7.5. ( Aa – Ac ) Cutaneous angiosarcoma. ( Ba – Bc ) Epithelioid haemangioendothelioma. ( Ca – Cc ) Testicular teratoma-derived angiosarcoma. ( Aa , Ba , Ca ) HE was used to identify structures. BV: healthy blood vessels; *: tumoral blood vessels. Scale bars 150 μm. Representative immunolabeling images showing merged Kv7.1 ( Ab , Bb , Cb ) and Kv7.5 ( Ac , Bc , Cc ) and tumor biopsies. Red, channels; green, <t>α-actin</t> (muscle); blue, DAPI nuclear staining. Scale bars 150 μm. ( D ) Tumor-to-normal (TN) ratios, in arbitrary units, were calculated from the protein expression levels obtained in immunofluorescence assays and represented in a forest plot. The analysis was performed for Kv7.1 ( top ) and Kv7.5 ( bottom ) channels, plotted in horizontal bars. The reference value (1) is indicated by a dotted line. Squares represent the mean ± SEM of the TN ratio for each patient and tumor. Gray, cutaneous angiosarcoma. White, epithelioid haemangioendothelioma. Black, testicular teratoma-derived angiosarcoma. Mean ± SEM, only represented when multiple samples and regions of interest (ROIs) were available.
    Anti α Actin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti α actin/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti α actin - by Bioz Stars, 2021-09
    80/100 stars
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    94
    Alomone Labs forskolin
    Neurite outgrowth is regulated by cAMP signaling. A. Plated neurospheres were treated with 10 μM <t>forskolin</t> for 24 h. The number of neurites crossing a circular mask around the neurosphere was used for quantification. B. Micrographs of control and 10 μM forskolin-treated neurospheres immunostained for beta III tubulin (green). Nuclei counterstained with DAPI (blue). C. Number of neurites crossing the circle (**p
    Forskolin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/forskolin/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    forskolin - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Kv7.1 and Kv7.5 expression in optimal cutting temperature compound (OCT)-embedded malignant vascular tumors. Consecutive sections of vascular tumor samples were stained by hematoxylin-eosin (HE) and immunofluorescent labeling for Kv7.1 and Kv7.5. ( Aa – Ac ) Cutaneous angiosarcoma. ( Ba – Bc ) Epithelioid haemangioendothelioma. ( Ca – Cc ) Testicular teratoma-derived angiosarcoma. ( Aa , Ba , Ca ) HE was used to identify structures. BV: healthy blood vessels; *: tumoral blood vessels. Scale bars 150 μm. Representative immunolabeling images showing merged Kv7.1 ( Ab , Bb , Cb ) and Kv7.5 ( Ac , Bc , Cc ) and tumor biopsies. Red, channels; green, α-actin (muscle); blue, DAPI nuclear staining. Scale bars 150 μm. ( D ) Tumor-to-normal (TN) ratios, in arbitrary units, were calculated from the protein expression levels obtained in immunofluorescence assays and represented in a forest plot. The analysis was performed for Kv7.1 ( top ) and Kv7.5 ( bottom ) channels, plotted in horizontal bars. The reference value (1) is indicated by a dotted line. Squares represent the mean ± SEM of the TN ratio for each patient and tumor. Gray, cutaneous angiosarcoma. White, epithelioid haemangioendothelioma. Black, testicular teratoma-derived angiosarcoma. Mean ± SEM, only represented when multiple samples and regions of interest (ROIs) were available.

    Journal: International Journal of Molecular Sciences

    Article Title: Remodeling of Kv7.1 and Kv7.5 Expression in Vascular Tumors

    doi: 10.3390/ijms21176019

    Figure Lengend Snippet: Kv7.1 and Kv7.5 expression in optimal cutting temperature compound (OCT)-embedded malignant vascular tumors. Consecutive sections of vascular tumor samples were stained by hematoxylin-eosin (HE) and immunofluorescent labeling for Kv7.1 and Kv7.5. ( Aa – Ac ) Cutaneous angiosarcoma. ( Ba – Bc ) Epithelioid haemangioendothelioma. ( Ca – Cc ) Testicular teratoma-derived angiosarcoma. ( Aa , Ba , Ca ) HE was used to identify structures. BV: healthy blood vessels; *: tumoral blood vessels. Scale bars 150 μm. Representative immunolabeling images showing merged Kv7.1 ( Ab , Bb , Cb ) and Kv7.5 ( Ac , Bc , Cc ) and tumor biopsies. Red, channels; green, α-actin (muscle); blue, DAPI nuclear staining. Scale bars 150 μm. ( D ) Tumor-to-normal (TN) ratios, in arbitrary units, were calculated from the protein expression levels obtained in immunofluorescence assays and represented in a forest plot. The analysis was performed for Kv7.1 ( top ) and Kv7.5 ( bottom ) channels, plotted in horizontal bars. The reference value (1) is indicated by a dotted line. Squares represent the mean ± SEM of the TN ratio for each patient and tumor. Gray, cutaneous angiosarcoma. White, epithelioid haemangioendothelioma. Black, testicular teratoma-derived angiosarcoma. Mean ± SEM, only represented when multiple samples and regions of interest (ROIs) were available.

    Article Snippet: After blocking for 1 h (5% nonfat powder milk, 10% goat serum, and 0.3% Triton X-100 in PBS-G), samples were incubated with anti-Kv7.1 (1:200, Alomone) or anti-Kv7.5 (1:200, Alomone) and anti-α-actin (1:500, Alomone) antibodies.

    Techniques: Expressing, Staining, Labeling, Derivative Assay, Immunolabeling, Immunofluorescence

    Voltage-dependent potassium (Kv)7.1 and Kv7.5 are differentially expressed in human blood vessels. ( A ) Membrane protein extracts were obtained from several human blood vessels (aorta and carotid arteries and inferior cava and subclavian veins) and rat tissue samples (brain, skeletal muscle and heart). ( B and C ) Fresh human samples were obtained and processed as described in the Materials and Methods. Sections were stained with anti-Kv7.1 ( B ) or anti-Kv7.5 ( C ) antibodies. Then, α-actin was used to identify muscle structures. Red, Kv7.1 ( B ) and Kv7.5 ( C ) channels; green, α-actin; merge, superimposed channel, α-actin and nuclear DAPI staining (in blue). TI: tunica intima; TM: tunica media; TE: tunica externa; e: endothelial layer; se: subendothelial layer. Scale bars: 50 μm in cava and subclavian veins; 100 μm in carotid artery; 250 μm in aorta artery.

    Journal: International Journal of Molecular Sciences

    Article Title: Remodeling of Kv7.1 and Kv7.5 Expression in Vascular Tumors

    doi: 10.3390/ijms21176019

    Figure Lengend Snippet: Voltage-dependent potassium (Kv)7.1 and Kv7.5 are differentially expressed in human blood vessels. ( A ) Membrane protein extracts were obtained from several human blood vessels (aorta and carotid arteries and inferior cava and subclavian veins) and rat tissue samples (brain, skeletal muscle and heart). ( B and C ) Fresh human samples were obtained and processed as described in the Materials and Methods. Sections were stained with anti-Kv7.1 ( B ) or anti-Kv7.5 ( C ) antibodies. Then, α-actin was used to identify muscle structures. Red, Kv7.1 ( B ) and Kv7.5 ( C ) channels; green, α-actin; merge, superimposed channel, α-actin and nuclear DAPI staining (in blue). TI: tunica intima; TM: tunica media; TE: tunica externa; e: endothelial layer; se: subendothelial layer. Scale bars: 50 μm in cava and subclavian veins; 100 μm in carotid artery; 250 μm in aorta artery.

    Article Snippet: After blocking for 1 h (5% nonfat powder milk, 10% goat serum, and 0.3% Triton X-100 in PBS-G), samples were incubated with anti-Kv7.1 (1:200, Alomone) or anti-Kv7.5 (1:200, Alomone) and anti-α-actin (1:500, Alomone) antibodies.

    Techniques: Staining

    Kv7.1 and Kv7.5 expression in paraffin-embedded vascular tumors. Consecutive sections of vascular tumor samples were stained with hematoxylin-eosin (HE) and were immunofluorescently labeled for Kv7.1 and Kv7.5. ( Aa – Ae ) Cavernous angioma. ( Ba – Be ) Capillary hemangioma. ( Ca – Ce ) Kaposi’s sarcoma. ( Da – De ) Angiosarcoma. ( Ea – Ee ) Glomus tumor. HE was used to identify structures. BV: healthy blood vessels; *: tumoral blood vessels. Scale bars: 50 μm, capillary angioma; 100 μm, cavernous angioma, Kaposi’s sarcoma and angiosarcoma; 200 μm, glomus tumor. Representative immunolabeling images showing merged Kv7.1 in healthy ( Ab , Bb , Cb , Db , Eb ) and tumor ( Ac , Bc , Cc , Dc , Ec ) biopsies. Representative immunolabeling images showing merged Kv7.5 in healthy (Ad, Bd, Cd, Dd, Ed) and tumor ( Ae , Be , Ce , De , Ee ) biopsies. Red, channels; green, α-actin (muscle); blue, DAPI nuclear staining; gray, far red detection of red blood cells showing high autofluorescence Scale bars: 100 μm in cavernous and capillary angioma; 80 μm in Kaposi’s sarcoma, angiosarcoma and glomus tumor.

    Journal: International Journal of Molecular Sciences

    Article Title: Remodeling of Kv7.1 and Kv7.5 Expression in Vascular Tumors

    doi: 10.3390/ijms21176019

    Figure Lengend Snippet: Kv7.1 and Kv7.5 expression in paraffin-embedded vascular tumors. Consecutive sections of vascular tumor samples were stained with hematoxylin-eosin (HE) and were immunofluorescently labeled for Kv7.1 and Kv7.5. ( Aa – Ae ) Cavernous angioma. ( Ba – Be ) Capillary hemangioma. ( Ca – Ce ) Kaposi’s sarcoma. ( Da – De ) Angiosarcoma. ( Ea – Ee ) Glomus tumor. HE was used to identify structures. BV: healthy blood vessels; *: tumoral blood vessels. Scale bars: 50 μm, capillary angioma; 100 μm, cavernous angioma, Kaposi’s sarcoma and angiosarcoma; 200 μm, glomus tumor. Representative immunolabeling images showing merged Kv7.1 in healthy ( Ab , Bb , Cb , Db , Eb ) and tumor ( Ac , Bc , Cc , Dc , Ec ) biopsies. Representative immunolabeling images showing merged Kv7.5 in healthy (Ad, Bd, Cd, Dd, Ed) and tumor ( Ae , Be , Ce , De , Ee ) biopsies. Red, channels; green, α-actin (muscle); blue, DAPI nuclear staining; gray, far red detection of red blood cells showing high autofluorescence Scale bars: 100 μm in cavernous and capillary angioma; 80 μm in Kaposi’s sarcoma, angiosarcoma and glomus tumor.

    Article Snippet: After blocking for 1 h (5% nonfat powder milk, 10% goat serum, and 0.3% Triton X-100 in PBS-G), samples were incubated with anti-Kv7.1 (1:200, Alomone) or anti-Kv7.5 (1:200, Alomone) and anti-α-actin (1:500, Alomone) antibodies.

    Techniques: Expressing, Staining, Labeling, Immunolabeling

    Neurite outgrowth is regulated by cAMP signaling. A. Plated neurospheres were treated with 10 μM forskolin for 24 h. The number of neurites crossing a circular mask around the neurosphere was used for quantification. B. Micrographs of control and 10 μM forskolin-treated neurospheres immunostained for beta III tubulin (green). Nuclei counterstained with DAPI (blue). C. Number of neurites crossing the circle (**p

    Journal: bioRxiv

    Article Title: Quantitative profiling of axonal guidance proteins during the differentiation of human neurospheres

    doi: 10.1101/2021.02.21.432144

    Figure Lengend Snippet: Neurite outgrowth is regulated by cAMP signaling. A. Plated neurospheres were treated with 10 μM forskolin for 24 h. The number of neurites crossing a circular mask around the neurosphere was used for quantification. B. Micrographs of control and 10 μM forskolin-treated neurospheres immunostained for beta III tubulin (green). Nuclei counterstained with DAPI (blue). C. Number of neurites crossing the circle (**p

    Article Snippet: 10-day old neurospheres were treated with 10 μM forskolin (Alomone Labs) 24 hours after plating and analyzed 24 h after treatment.

    Techniques:

    At low depolarisation K v 7 channel inhibition reduces the response to forskolin. Strips pre-constricted with 20 mM KPSS were treated with either 0.1 μM isoprenaline (A) or 1 μM forskolin (B). Pre-treatment with K v 7 channel modulators revealed a reduction in response to isoprenaline and forskolin when blocking K v 7 channels by XE991 (10 μM) whereas retigabine (10μM) did not affect the response to isoprenaline. Pre-treatment with vehicle control (0.1% DMSO). ** P

    Journal: PLoS ONE

    Article Title: Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction

    doi: 10.1371/journal.pone.0117350

    Figure Lengend Snippet: At low depolarisation K v 7 channel inhibition reduces the response to forskolin. Strips pre-constricted with 20 mM KPSS were treated with either 0.1 μM isoprenaline (A) or 1 μM forskolin (B). Pre-treatment with K v 7 channel modulators revealed a reduction in response to isoprenaline and forskolin when blocking K v 7 channels by XE991 (10 μM) whereas retigabine (10μM) did not affect the response to isoprenaline. Pre-treatment with vehicle control (0.1% DMSO). ** P

    Article Snippet: Organ bath studies Retigabine and forskolin were purchased from Alamone Labs (Jerusalem, Israel) whereas XE991 was kindly provided by NeuroSearch (Ballerup, Denmark), and ML213 and ML277 were kindly provided by Vanderbilt University (Nashville, TN, USA (MLCPN probes)).

    Techniques: Inhibition, Blocking Assay

    Modulation of K v 7 channels does not affect Gs mediated relaxation at 1 μM carbachol-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 1 μM carbachol. * P

    Journal: PLoS ONE

    Article Title: Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction

    doi: 10.1371/journal.pone.0117350

    Figure Lengend Snippet: Modulation of K v 7 channels does not affect Gs mediated relaxation at 1 μM carbachol-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 1 μM carbachol. * P

    Article Snippet: Organ bath studies Retigabine and forskolin were purchased from Alamone Labs (Jerusalem, Israel) whereas XE991 was kindly provided by NeuroSearch (Ballerup, Denmark), and ML213 and ML277 were kindly provided by Vanderbilt University (Nashville, TN, USA (MLCPN probes)).

    Techniques: Produced, Incubation

    Modulation of K v 7 channels does not affect Gs mediated relaxation at 40 mM KPSS-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 40 mM KPSS. Neither of the K v 7 channel modulators affected the response to isoprenaline or forskolin.

    Journal: PLoS ONE

    Article Title: Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction

    doi: 10.1371/journal.pone.0117350

    Figure Lengend Snippet: Modulation of K v 7 channels does not affect Gs mediated relaxation at 40 mM KPSS-induced contractility. Cumulative concentrations-responses for isoprenaline (A and B) and forskolin (C) were produced with pre-incubation of 10 μM XE991 (A and C), 10 μM retigabine (B) or vehicle control (0.1% DMSO). Initial tone was achieved by pre-constriction with 40 mM KPSS. Neither of the K v 7 channel modulators affected the response to isoprenaline or forskolin.

    Article Snippet: Organ bath studies Retigabine and forskolin were purchased from Alamone Labs (Jerusalem, Israel) whereas XE991 was kindly provided by NeuroSearch (Ballerup, Denmark), and ML213 and ML277 were kindly provided by Vanderbilt University (Nashville, TN, USA (MLCPN probes)).

    Techniques: Produced, Incubation

    Effect of isoprenaline and forskolin in human DSM at 1 μM carbachol- and 40 mM KPSS-induced contractions. Cumulative concentration-responses were induced with isoprenaline and forskolin. Initial tone was achieved by pre-constriction with 1 μM carbachol (A and B) and 40 mM KPSS (C and D) bladder strips. * P

    Journal: PLoS ONE

    Article Title: Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction

    doi: 10.1371/journal.pone.0117350

    Figure Lengend Snippet: Effect of isoprenaline and forskolin in human DSM at 1 μM carbachol- and 40 mM KPSS-induced contractions. Cumulative concentration-responses were induced with isoprenaline and forskolin. Initial tone was achieved by pre-constriction with 1 μM carbachol (A and B) and 40 mM KPSS (C and D) bladder strips. * P

    Article Snippet: Organ bath studies Retigabine and forskolin were purchased from Alamone Labs (Jerusalem, Israel) whereas XE991 was kindly provided by NeuroSearch (Ballerup, Denmark), and ML213 and ML277 were kindly provided by Vanderbilt University (Nashville, TN, USA (MLCPN probes)).

    Techniques: Concentration Assay

    CREB phosphorylation mediates AKT2-regulated induction of PEPCK. ( A ) CREB phosphorylation induced by PTEN loss is regulated by AKT2. Liver lysates from mice with different genotypes were analysis for p-CREB. PM, Pten deleted; DM, Pten and Akt2 deleted; AM, Akt2 deleted. Two biological replicas are included for each genotype group. Protein levels of p-AKT, PTEN and p-GSK3 are analyzed and showed up-regulation of AKT and GSK-3 with PTEN loss and inhibition when AKT2 is lost. Phosphorylation of CREB is only observed when PTEN is lost. AKT2 loss together with PTEN blocked this phosphorylation in the DM livers. ( B ) Akt2 +/+ and Akt2 − / − cell were treated with forskolin (100 μM) for 24 h to induce cAMP and CREB phosphorylation or vehicle as controls. Phosphorylation of CREB is detected and showed to be lower when AKT2 is lost. When forskolin is added to induce cAMP, protein expression of p-CREB is evaluated in both Akt2 +/+ and Akt2 − / − cells even though its levels remains lower in the Akt2 − / − cells. Vinculin is probed as loading control. ( C ) Introduction of CREB-133A and wtCREB to Akt2 +/+ and Akt2 −/− hepatocytes. Expression of PEPCK is analyzed using quantitative PCR analysis. Left panel, CREB-S133A is introduced to Akt2 +/+ cells. Middle panel, wtCREB is introduced to Akt2 −/− cells; Right panel, wtCREB is introduced to Akt2 +/+ cells. n = 3. Data presented as mean ± SEM. * P ≤ 0.05. Different from vector transfected cells of the same genotype. Experiments repeated at least three times.

    Journal: The Biochemical journal

    Article Title: Regulation of basal expression of hepatic PEPCK and G6Pase by AKT2

    doi: 10.1042/BCJ20190570

    Figure Lengend Snippet: CREB phosphorylation mediates AKT2-regulated induction of PEPCK. ( A ) CREB phosphorylation induced by PTEN loss is regulated by AKT2. Liver lysates from mice with different genotypes were analysis for p-CREB. PM, Pten deleted; DM, Pten and Akt2 deleted; AM, Akt2 deleted. Two biological replicas are included for each genotype group. Protein levels of p-AKT, PTEN and p-GSK3 are analyzed and showed up-regulation of AKT and GSK-3 with PTEN loss and inhibition when AKT2 is lost. Phosphorylation of CREB is only observed when PTEN is lost. AKT2 loss together with PTEN blocked this phosphorylation in the DM livers. ( B ) Akt2 +/+ and Akt2 − / − cell were treated with forskolin (100 μM) for 24 h to induce cAMP and CREB phosphorylation or vehicle as controls. Phosphorylation of CREB is detected and showed to be lower when AKT2 is lost. When forskolin is added to induce cAMP, protein expression of p-CREB is evaluated in both Akt2 +/+ and Akt2 − / − cells even though its levels remains lower in the Akt2 − / − cells. Vinculin is probed as loading control. ( C ) Introduction of CREB-133A and wtCREB to Akt2 +/+ and Akt2 −/− hepatocytes. Expression of PEPCK is analyzed using quantitative PCR analysis. Left panel, CREB-S133A is introduced to Akt2 +/+ cells. Middle panel, wtCREB is introduced to Akt2 −/− cells; Right panel, wtCREB is introduced to Akt2 +/+ cells. n = 3. Data presented as mean ± SEM. * P ≤ 0.05. Different from vector transfected cells of the same genotype. Experiments repeated at least three times.

    Article Snippet: The medium was replaced with fresh medium with 100 μM Forskolin (Alomone Labs Cat# F-500) or vehicle and incubated for 24 h before harvesting.

    Techniques: Mouse Assay, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Transfection