Journal: The Journal of Neuroscience
Article Title: Development of Survival Responsiveness to Brain-Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5, But Not to Nerve Growth Factor, in Cultured Motoneurons from Chick Embryo Spinal Cord
Figure Lengend Snippet: The survival response of MTNs to NTs varies with time in culture. MTNs were purified from E5.5 chick embryos and cultured in the presence of MEX (300 μg/ml) for 24 hr ( black bars ) or 72 hr ( white bars ), whereupon cultures were rinsed three times
Article Snippet: Tyrosine autophosphorylation of Trks, during binding of the specific human recombinant NTs (Alomone Laboratories, Jerusalem, Israel) or after MEX stimulation was monitored by immunoblotting with the anti-phosphotyrosine monoclonal antibody 4G10 (kindly provided by Dr. David Kaplan, Montreal Neurological Institute, Montreal, Canada, or obtained from UBI, Lake Placid, NY).
Techniques: Purification, Cell Culture