compound fpl64176  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Alomone Labs compound fpl64176
    BayK 8644 and FPL 64176 increased Ca 2+  spark frequency in permeabilized myocytes A (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of BayK 8644 (BayK, 1 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Numerical data of Ca 2+  release events (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of BayK 8644 (red).  B (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of FPL 64176 (FPL, 5 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Average data of Ca 2+  sparks (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of FPL (red). * Statistically different at P
    Compound Fpl64176, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/compound fpl64176/product/Alomone Labs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    compound fpl64176 - by Bioz Stars, 2022-12
    93/100 stars

    Images

    1) Product Images from "Ca2+ entry-independent effects of L-type Ca2+ channel modulators on Ca2+ sparks in ventricular myocytes"

    Article Title: Ca2+ entry-independent effects of L-type Ca2+ channel modulators on Ca2+ sparks in ventricular myocytes

    Journal: American journal of physiology. Cell physiology

    doi: 10.1152/ajpcell.00437.2006

    BayK 8644 and FPL 64176 increased Ca 2+  spark frequency in permeabilized myocytes A (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of BayK 8644 (BayK, 1 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Numerical data of Ca 2+  release events (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of BayK 8644 (red).  B (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of FPL 64176 (FPL, 5 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Average data of Ca 2+  sparks (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of FPL (red). * Statistically different at P
    Figure Legend Snippet: BayK 8644 and FPL 64176 increased Ca 2+ spark frequency in permeabilized myocytes A (a) Confocal linescan images of Ca 2+ sparks in control conditions and in the presence of BayK 8644 (BayK, 1 μM). Bottom traces are localΔF/F 0 profiles of Ca 2+ release events in the region marked by the boxes. (b, c, d, e) Numerical data of Ca 2+ release events (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of BayK 8644 (red). B (a) Confocal linescan images of Ca 2+ sparks in control conditions and in the presence of FPL 64176 (FPL, 5 μM). Bottom traces are localΔF/F 0 profiles of Ca 2+ release events in the region marked by the boxes. (b, c, d, e) Average data of Ca 2+ sparks (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of FPL (red). * Statistically different at P

    Techniques Used:

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Alomone Labs compound fpl64176
    BayK 8644 and FPL 64176 increased Ca 2+  spark frequency in permeabilized myocytes A (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of BayK 8644 (BayK, 1 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Numerical data of Ca 2+  release events (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of BayK 8644 (red).  B (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of FPL 64176 (FPL, 5 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Average data of Ca 2+  sparks (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of FPL (red). * Statistically different at P
    Compound Fpl64176, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/compound fpl64176/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    compound fpl64176 - by Bioz Stars, 2022-12
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs anti kv1 5 antibody
    Mutant <t>KCNA5</t> is located in perinuclear packets and not on the cell surface of transfected HEK-293 cells and human (h)PASMC. A : HEK-293 cells transfected with WT KCNA5 ( a and c ) or G182R ( b and d ) were stained with anti-KCNA5 antibody (Ab-KCNA5, red) and
    Anti Kv1 5 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 5 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 5 antibody - by Bioz Stars, 2022-12
    93/100 stars
      Buy from Supplier

    Image Search Results


    BayK 8644 and FPL 64176 increased Ca 2+  spark frequency in permeabilized myocytes A (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of BayK 8644 (BayK, 1 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Numerical data of Ca 2+  release events (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of BayK 8644 (red).  B (a)  Confocal linescan images of Ca 2+  sparks in control conditions and in the presence of FPL 64176 (FPL, 5 μM). Bottom traces are localΔF/F 0  profiles of Ca 2+  release events in the region marked by the boxes.  (b, c, d, e)  Average data of Ca 2+  sparks (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of FPL (red). * Statistically different at P

    Journal: American journal of physiology. Cell physiology

    Article Title: Ca2+ entry-independent effects of L-type Ca2+ channel modulators on Ca2+ sparks in ventricular myocytes

    doi: 10.1152/ajpcell.00437.2006

    Figure Lengend Snippet: BayK 8644 and FPL 64176 increased Ca 2+ spark frequency in permeabilized myocytes A (a) Confocal linescan images of Ca 2+ sparks in control conditions and in the presence of BayK 8644 (BayK, 1 μM). Bottom traces are localΔF/F 0 profiles of Ca 2+ release events in the region marked by the boxes. (b, c, d, e) Numerical data of Ca 2+ release events (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of BayK 8644 (red). B (a) Confocal linescan images of Ca 2+ sparks in control conditions and in the presence of FPL 64176 (FPL, 5 μM). Bottom traces are localΔF/F 0 profiles of Ca 2+ release events in the region marked by the boxes. (b, c, d, e) Average data of Ca 2+ sparks (frequency, amplitude, duration and width respectively) under control conditions (black) and in the presence of FPL (red). * Statistically different at P

    Article Snippet: Nifedipine, nimodipine, FPL-64176 and Bay-K8644 were from Alomone Lab, or Sigma Chemical Company (St. Louis, MO, USA).

    Techniques:

    Mutant KCNA5 is located in perinuclear packets and not on the cell surface of transfected HEK-293 cells and human (h)PASMC. A : HEK-293 cells transfected with WT KCNA5 ( a and c ) or G182R ( b and d ) were stained with anti-KCNA5 antibody (Ab-KCNA5, red) and

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Mutant KCNA5 is located in perinuclear packets and not on the cell surface of transfected HEK-293 cells and human (h)PASMC. A : HEK-293 cells transfected with WT KCNA5 ( a and c ) or G182R ( b and d ) were stained with anti-KCNA5 antibody (Ab-KCNA5, red) and

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Mutagenesis, Transfection, Staining

    G182R and E211D mutations cause incomplete processing of KCNA5 in HEK-293 cells. HEK-293 cells were transfected with WT KCNA5, G182R, E211D, or G182R/E211D and subjected to standard immunoblot procedures. A : representative immunoblot from cells transfected

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: G182R and E211D mutations cause incomplete processing of KCNA5 in HEK-293 cells. HEK-293 cells were transfected with WT KCNA5, G182R, E211D, or G182R/E211D and subjected to standard immunoblot procedures. A : representative immunoblot from cells transfected

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Transfection

    Mutant KCNA5 forms functional homotetrameric channels. A : COS-1, HEK-293, and human pulmonary artery smooth muscle cells (PASMC) were transiently transfected with either empty vector [green fluorescent protein (GFP)] or WT KCNA5 (WT) as indicated. Whole

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Mutant KCNA5 forms functional homotetrameric channels. A : COS-1, HEK-293, and human pulmonary artery smooth muscle cells (PASMC) were transiently transfected with either empty vector [green fluorescent protein (GFP)] or WT KCNA5 (WT) as indicated. Whole

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Mutagenesis, Functional Assay, Transfection, Plasmid Preparation

    Mutations in KCNA5 at G182 and E211 do not affect the pharmacological effect of 4-aminopyridine (4-AP). A : a standard I-V pulse protocol was delivered to HEK-293 cells transiently transfected with the indicated vector [WT KCNA5 ( a ), G182R ( b ), E211D (

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Mutations in KCNA5 at G182 and E211 do not affect the pharmacological effect of 4-aminopyridine (4-AP). A : a standard I-V pulse protocol was delivered to HEK-293 cells transiently transfected with the indicated vector [WT KCNA5 ( a ), G182R ( b ), E211D (

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Transfection, Plasmid Preparation

    Decreased G182R expression in COS-1 cells cannot be rescued by overexpression of K V β subunits. A , a : HEK-293 cells were transfected with WT KCNA5 or K V β1.3-HA alone or cotransfected with WT KCNA5, G182R, E211D, or G182R/E211D and K V β1.3-HA.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Decreased G182R expression in COS-1 cells cannot be rescued by overexpression of K V β subunits. A , a : HEK-293 cells were transfected with WT KCNA5 or K V β1.3-HA alone or cotransfected with WT KCNA5, G182R, E211D, or G182R/E211D and K V β1.3-HA.

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Expressing, Over Expression, Transfection

    Two nonsynonymous mutations identified in the KCNA5 gene from idiopathic pulmonary arterial hypertension (IPAH) patients localize to the NH 2 -terminal tetramerization domain (T1 domain). A , left : schematic diagram of voltage-gated K + (K V ) channel subunit

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Two nonsynonymous mutations identified in the KCNA5 gene from idiopathic pulmonary arterial hypertension (IPAH) patients localize to the NH 2 -terminal tetramerization domain (T1 domain). A , left : schematic diagram of voltage-gated K + (K V ) channel subunit

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques:

    Cotransfection of K V β subunits affects KCNA5 channel kinetics. HEK-293 cells were transfected with WT KCNA5 alone (KCNA5) or in the presence of K V β1.3-hemagglutinin (HA) (KCNA5/K V β1.3). A and B : representative current recordings

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Cotransfection of K V β subunits affects KCNA5 channel kinetics. HEK-293 cells were transfected with WT KCNA5 alone (KCNA5) or in the presence of K V β1.3-hemagglutinin (HA) (KCNA5/K V β1.3). A and B : representative current recordings

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Cotransfection, Transfection

    Voltage-dependent inactivation is accelerated in the G182R mutant KCNA5 channel. A standard 2-pulse inactivation protocol was used to determine channel availability after a 10-s prepulse in HEK-293 cells transiently transfected with WT KCNA5, G182R, E211D,

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: Voltage-dependent inactivation is accelerated in the G182R mutant KCNA5 channel. A standard 2-pulse inactivation protocol was used to determine channel availability after a 10-s prepulse in HEK-293 cells transiently transfected with WT KCNA5, G182R, E211D,

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Mutagenesis, Transfection

    G182R protein expression is significantly decreased in COS-1 cells. A : COS-1 cells were transiently transfected with water (Mock), WT-KCNA5, G182R, E211D, or G182R/E211D. Representative images are shown at ×40 magnification. B : transfected cells

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns

    doi: 10.1152/ajpcell.00464.2009

    Figure Lengend Snippet: G182R protein expression is significantly decreased in COS-1 cells. A : COS-1 cells were transiently transfected with water (Mock), WT-KCNA5, G182R, E211D, or G182R/E211D. Representative images are shown at ×40 magnification. B : transfected cells

    Article Snippet: The rest of the lysate was incubated with 1:160 anti-KV1.5 antibody (Alomone) for 45 min on ice.

    Techniques: Expressing, Transfection