Mmp 9 Elisa, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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1) Product Images from "Filifactor alocis modulates human neutrophil antimicrobial functional responses"
Article Title: Filifactor alocis modulates human neutrophil antimicrobial functional responses
Journal: Cellular microbiology
Figure Legend Snippet: Opsonized F. alocis induces exocytosis of three of the four neutrophil granule subtypes Neutrophils were unchallenged (basal), challenged with fMLF (5 min), or challenged with live or heat killed (HK) opsonized F. alocis (MOI 10, 30 min). A-B . Secretory vesicle and specific granule exocytosis was determined via flow cytometry by measuring the increase in plasma membrane expression of CD35 or CD66b respectively. Data are expressed as the mean ± SEM of the mean channel of fluorescence (mcf) from 6 independent experiments. In A , ns: non-significant compared to basal. C . Gelatinase granule exocytosis was measured by ELISA as levels of matrix metalloproteinase 9 (MMP-9) present in the supernatants collected from the different experimental conditions. Data are expressed as mean ± SEM of MMP-9 release in ng/4 × 10 6 cells, from 5 independent experiments. D. Gelatin zymography gels were used to determine the activity of MMP-9 in the supernatants from the same experimental conditions described above. The protein standard is shown on the left side, and the different MMP-9 isoforms are also indicated. E. Quantification of the densitometry values of the gel degradation corresponding to the 92 kDa band was analyzed by ImageJ software, from the gels of 4 independent experiments. F. Azurophil granule exocytosis was determined by the increase in plasma membrane expression of the CD63 marker by flow cytometry. Data are expressed as the mean ± SEM of the mean channel of fluorescence (mcf) from 5 independent experiments. *p
Techniques Used: Flow Cytometry, Cytometry, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Zymography, Activity Assay, Software, Marker
2) Product Images from "Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b"
Article Title: Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b
Figure Legend Snippet: P85α deletion converts fibroblasts into activated myofibroblasts exhibiting features of CAFs. ( a ) Western blot analysis of p85α, phospho-Akt, Akt and α-smooth muscle actin (α-SMA) expression in WT and p85α −/− fibroblasts. p-Akt, Phospho-Akt (Ser473); T-Akt, Akt (pan). ( b ) Immunofluorescence analysis of α-SMA expression in WT and p85α −/− fibroblasts. Scale bar, 50μm. ( c ) The area of the contracted gels induced by WT or p85α −/− fibroblasts. The appearance of the contracted gels that have four replications at 72 h is also shown. ( d ) The morphology of WT or p85α −/− fibroblasts in a dish or collagen lattice described in ( c ). ( e ) Immunofluorescence, western blot and fluorescence-activated cell sorting (FACS) analysis of CD44 expression in WT and p85α −/− fibroblasts. Scale bar, 20 μm. ( f ) Reverse transcription–PCR analysis of TGF-β family proteins and SDF-1. MMP2 and MMP9 expression in WT and p85α −/− fibroblasts. ( g ) Quantitative PCR analysis of TGF-β1, SDF-1, MMP2 and MMP9 expression in WT and p85α −/− fibroblasts. Data are the mean±s.e.m. ( h ) Enzyme-linked immunosorbent assay (ELISA) analysis of TGF-β1, SDF-1, MMP2 and MMP9 expression in conditioned medium of WT and p85α −/− fibroblasts. Data are the mean±s.e.m. ** P- value⩽0.01. ( i ) ELISA analysis of TGF-β1, SDF-1, MMP2 and MMP9 expression in p85α −/− fibroblast-conditioned medium of when treated with or without the PI3K inhibitor LY294002 (50 μ M ) for 24 h and the DMSO solution as a control. Con, p85α −/− fibroblasts treated with DMSO, LY, p85α −/− fibroblasts treated with LY294002. Data are the mean±s.e.m. ** P- value⩽0.01.
Techniques Used: Western Blot, Expressing, Immunofluorescence, Fluorescence, FACS, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay