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human elisa kit  (Boster Bio)


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    Boster Bio human elisa kit
    Human Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human elisa kit/product/Boster Bio
    Average 94 stars, based on 40 article reviews
    human elisa kit - by Bioz Stars, 2026-02
    94/100 stars

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    Effect of MDA-7/IL-24 on the expression of the IL-1β and the IL-6. Expression levels were estimated by real-time quantitative PCR (IL-1β and IL-6 genes) and <t>ELISA</t> (IL-6) in normal LX-2 cells, 48 h (plasmids) or 24 h (protein) after the transfection with pcDNA 3.1, PMDA-7, PMDA-7-RGD, and treatment with IL-24 protein (100 ng/ml). ( A ) IL-1βmRNA expression levels in LX-2 cells. ( B ) The effect of MDA-7/IL-24 on the IL-6 mRNA expression levels. ( C ) An increase in the amount of IL-6 protein by ELISA at 48 h following administration of MDA-7/IL-24 was observed. Data (mean ± standard error of the mean) are shown. The analysis of ELISA and reverse transcription-quantitative polymerase chain reaction demonstrated that MDA-7/IL-24 increased IL-1β and IL-6 expression. The cell group treated with leptin significantly differed from the control group in all the genes studied. *P < 0.05, **P < 0.01 vs. control.
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    Effect of MDA-7/IL-24 on the expression of the IL-1β and the IL-6. Expression levels were estimated by real-time quantitative PCR (IL-1β and IL-6 genes) and ELISA (IL-6) in normal LX-2 cells, 48 h (plasmids) or 24 h (protein) after the transfection with pcDNA 3.1, PMDA-7, PMDA-7-RGD, and treatment with IL-24 protein (100 ng/ml). ( A ) IL-1βmRNA expression levels in LX-2 cells. ( B ) The effect of MDA-7/IL-24 on the IL-6 mRNA expression levels. ( C ) An increase in the amount of IL-6 protein by ELISA at 48 h following administration of MDA-7/IL-24 was observed. Data (mean ± standard error of the mean) are shown. The analysis of ELISA and reverse transcription-quantitative polymerase chain reaction demonstrated that MDA-7/IL-24 increased IL-1β and IL-6 expression. The cell group treated with leptin significantly differed from the control group in all the genes studied. *P < 0.05, **P < 0.01 vs. control.

    Journal: Scientific Reports

    Article Title: Pro-inflammatory effects of endogenous and recombinant MDA-7/IL-24 with RGD peptide on human hepatic stellate cells

    doi: 10.1038/s41598-025-06850-9

    Figure Lengend Snippet: Effect of MDA-7/IL-24 on the expression of the IL-1β and the IL-6. Expression levels were estimated by real-time quantitative PCR (IL-1β and IL-6 genes) and ELISA (IL-6) in normal LX-2 cells, 48 h (plasmids) or 24 h (protein) after the transfection with pcDNA 3.1, PMDA-7, PMDA-7-RGD, and treatment with IL-24 protein (100 ng/ml). ( A ) IL-1βmRNA expression levels in LX-2 cells. ( B ) The effect of MDA-7/IL-24 on the IL-6 mRNA expression levels. ( C ) An increase in the amount of IL-6 protein by ELISA at 48 h following administration of MDA-7/IL-24 was observed. Data (mean ± standard error of the mean) are shown. The analysis of ELISA and reverse transcription-quantitative polymerase chain reaction demonstrated that MDA-7/IL-24 increased IL-1β and IL-6 expression. The cell group treated with leptin significantly differed from the control group in all the genes studied. *P < 0.05, **P < 0.01 vs. control.

    Article Snippet: Nearly 48 h after LX-2 cell transfection and treatment with plasmids and IL-24 protein, respectively, the culture supernatants were collected for measurement of the released IL-6 by a human IL-6 ELISA Kit (Boster Biological Technology Co., Ltd., Pleasanton, CA, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection, Reverse Transcription, Control