collagenase  (Worthington Biochemical)


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    • 99
    Name:
    Collagenase Type 3
    Description:
    Lower in secondary proteolytic contaminant activities but with typical collagenase activity Suggested for mammary primary cell isolation A dialyzed lyophilized powder
    Catalog Number:
    LS004180
    Price:
    35
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
    		Buy from Supplier

    Structured Review

    Worthington Biochemical collagenase
    Lower in secondary proteolytic contaminant activities but with typical collagenase activity Suggested for mammary primary cell isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Ly6C- Monocytes Regulate Parasite-Induced Liver Inflammation by Inducing the Differentiation of Pathogenic Ly6C+ Monocytes into Macrophages
    Article Snippet: At day 4 post infection, mice were bled, parasites were purified by diethyl-aminoethyl-cellulose chromatography and used to infect experimental female, age-matched (8–14 weeks) mice (2000 parasites/mouse, ip). .. Isolation of liver non-parenchymal immune cells and adoptive transfer of monocytes Animals were euthanized (CO2 ) and livers were perfused through the portal vein with 10 ml of 120 U/ml collagenase type III (Worthington Biochemical Corporation) in HBSS (Gibco). .. Then, liver was disrupted mechanically and incubated in 10 ml of a 100 U/ml collagenase III solution in HBSS (25 min, 37°C).

    Adoptive Transfer Assay:

    Article Title: Ly6C- Monocytes Regulate Parasite-Induced Liver Inflammation by Inducing the Differentiation of Pathogenic Ly6C+ Monocytes into Macrophages
    Article Snippet: At day 4 post infection, mice were bled, parasites were purified by diethyl-aminoethyl-cellulose chromatography and used to infect experimental female, age-matched (8–14 weeks) mice (2000 parasites/mouse, ip). .. Isolation of liver non-parenchymal immune cells and adoptive transfer of monocytes Animals were euthanized (CO2 ) and livers were perfused through the portal vein with 10 ml of 120 U/ml collagenase type III (Worthington Biochemical Corporation) in HBSS (Gibco). .. Then, liver was disrupted mechanically and incubated in 10 ml of a 100 U/ml collagenase III solution in HBSS (25 min, 37°C).

    Protease Inhibitor:

    Article Title: A rigorous method to enrich for exosomes from brain tissue
    Article Snippet: .. Reagents and chemicals Hibernate®-E Medium and Dulbecco’s phosphate-buffered saline (PBS) were from ThermoFisher, PhosSTOP (PS) & Complete Protease Inhibitor (including EDTA) (PI) and D2 O were from SigmaAldrich, collagenase type 3 (Worthington #CLS-3) and RNase A from Qiagen. .. Chemicals for proteomics were from SigmaAldrich.

    Flow Cytometry:

    Article Title: Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Article Snippet: Senescence associated β-galactosidase staining Ten micrometres of frozen sections of OCT-embedded tumours were stained for the senescence marker SA β-Gal according to the manufacturer's instructions (Cell Signaling) for B16F10 tumours the staining was performed with dodecanoylaminofluorescein di-β-D -galactopyranoside (C12FDG; Life Technologies) at 33 μM during 2 h at 37 °C at pH 6.0. .. Flow cytometry analysis Tumours were digested with 0.1% collagenase type III (Worthington) and 1 U ml−1 DNAse I (Promega) and incubated at 37 °C for 1 h. One hundred and six tumour cells were incubated with Fc-Block (BD Biosciences, #553141 at 1/100) before labelling with fluorochrome-conjugated antibodies. .. The following fluorochrome-conjugated antibodies were used: anti-NK1.1 (BD Biosciences, #557391 at 1/50); anti-CD335 (NKp46, eBioscience, #25–3351 at 1/50), anti-CD45 (eBioscience, #45–0451 at 1/50), anti-CD4 (Biolegend, #100408, at 1/50), anti-CD8 (Biolegend, #100723 at 1/50) and anti-F4/80 (Biolegend, #123118 at 1/50).

    Cytometry:

    Article Title: Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Article Snippet: Senescence associated β-galactosidase staining Ten micrometres of frozen sections of OCT-embedded tumours were stained for the senescence marker SA β-Gal according to the manufacturer's instructions (Cell Signaling) for B16F10 tumours the staining was performed with dodecanoylaminofluorescein di-β-D -galactopyranoside (C12FDG; Life Technologies) at 33 μM during 2 h at 37 °C at pH 6.0. .. Flow cytometry analysis Tumours were digested with 0.1% collagenase type III (Worthington) and 1 U ml−1 DNAse I (Promega) and incubated at 37 °C for 1 h. One hundred and six tumour cells were incubated with Fc-Block (BD Biosciences, #553141 at 1/100) before labelling with fluorochrome-conjugated antibodies. .. The following fluorochrome-conjugated antibodies were used: anti-NK1.1 (BD Biosciences, #557391 at 1/50); anti-CD335 (NKp46, eBioscience, #25–3351 at 1/50), anti-CD45 (eBioscience, #45–0451 at 1/50), anti-CD4 (Biolegend, #100408, at 1/50), anti-CD8 (Biolegend, #100723 at 1/50) and anti-F4/80 (Biolegend, #123118 at 1/50).

    Incubation:

    Article Title: Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Article Snippet: Senescence associated β-galactosidase staining Ten micrometres of frozen sections of OCT-embedded tumours were stained for the senescence marker SA β-Gal according to the manufacturer's instructions (Cell Signaling) for B16F10 tumours the staining was performed with dodecanoylaminofluorescein di-β-D -galactopyranoside (C12FDG; Life Technologies) at 33 μM during 2 h at 37 °C at pH 6.0. .. Flow cytometry analysis Tumours were digested with 0.1% collagenase type III (Worthington) and 1 U ml−1 DNAse I (Promega) and incubated at 37 °C for 1 h. One hundred and six tumour cells were incubated with Fc-Block (BD Biosciences, #553141 at 1/100) before labelling with fluorochrome-conjugated antibodies. .. The following fluorochrome-conjugated antibodies were used: anti-NK1.1 (BD Biosciences, #557391 at 1/50); anti-CD335 (NKp46, eBioscience, #25–3351 at 1/50), anti-CD45 (eBioscience, #45–0451 at 1/50), anti-CD4 (Biolegend, #100408, at 1/50), anti-CD8 (Biolegend, #100723 at 1/50) and anti-F4/80 (Biolegend, #123118 at 1/50).

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    • collagenase  (Worthington Biochemical)
    99
    Worthington Biochemical collagenase
    Collagenase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2021-04
    99/100 stars
    		  Buy from Supplier

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