collagenase (Worthington Biochemical)

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Worthington Biochemical collagenase
Collagenase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase/product/Worthington Biochemical
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99

collagenase - by Bioz Stars, 2021-10

94/100 stars

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Article Title: Enrichment of neutrophils and monocytes from the liver following either oral or intravenous Listeria monocytogenes infection
Article Snippet: Suspend 250 U/mL type IV collagenase (Worthington Biochemical cat. no. ) in HBSS with Ca++ and Mg++ (Life Tech. cat no. 14025) and filter sterilize.

Article Title: Enrichment of neutrophils and monocytes from the liver following either oral or intravenous Listeria monocytogenes infection
Article Snippet: 100 U/mL type IV collagenase (Worthington Biochemical cat. no. ).

FACS:

Article Title: Normalization of non‐canonical Wnt signalings does not compromise blood‐brain barrier protection conferred by upregulating endothelial Wnt/β‐catenin signaling following ischemic stroke
Article Snippet: .. 2.8 FACS sorting of brain ECs Brains of adult mice were minced and disaggregated in Type IV collagenase (400 U/ml, Worthington), Dispase (1.2 U/ml, Worthington), and DNase I (32 U/ml, Worthington) in PBS with Ca2+ and Mg2+ (GIBCO) at 37°C for 60 min, with gentle trituration every 10 min. After that, the disaggregated cells were washed with cold PBS and filtered through a 40‐μm mesh. ..

Mouse Assay:

Incubation:

Article Title: Loss of Aryl Hydrocarbon Receptor Favors K-RasG12D-Driven Non-Small Cell Lung Cancer
Article Snippet: .. Lung pieces were then dissociated into single cell suspensions by incubation in PBS-containing dispase (0.6 U/mL) (Roche, Basel, Switzerland), type-IV collagenase (60 U/mL) (Worthington, NJ, USA) and 0.0001% DNase solution (Roche) for 1 h at 37 °C with gentle shaking. ..

Isolation:

Article Title: mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation
Article Snippet: .. Isolated muscles were digested with 400 U/ml Type I collagenase (Worthington) in DMEM at 37 °C, till single fibers dissociated. ..