mouse sdc3 elisa kit picokine (Boster Bio)
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Mouse Sdc3 Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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1) Product Images from "Syndecan-3 as a Novel Biomarker in Alzheimer’s Disease"
Article Title: Syndecan-3 as a Novel Biomarker in Alzheimer’s Disease
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms23063407
Figure Legend Snippet: SDC3 expression of TNF-α-treated SH-SY5Y and hCMEC/D3 cells. Cells were incubated with or without (i.e., controls) 5 ng/mL TNF-α for 7 days. After incubation, the cells were treated with APC-labeled SDC3 antibody, and SDC3 was measured with imaging flow cytometry. ( A ) Representative flow cytometry histograms showing the SDC3 expression of SH-SY5Y and hCMEC/D3 cells. ( B ) Brightfield (BF) and fluorescent cellular images of SH-SY5Y and hCMEC/D3 cells treated with APC-labeled SDC3 antibody. Scale bar = 20 μm. ( C ) Detected fluorescence intensities were normalized control cells untreated with TNF-α. The bars represent the mean + SEM of ten independent experiments. Statistical significance vs. controls was assessed with ANOVA. *** p < 0.001.
Techniques Used: Expressing, Incubation, Labeling, Imaging, Flow Cytometry, Fluorescence, Control
Figure Legend Snippet: APPSWE-Tau mice exhibits increased TNF-α concentrations in the brain and the blood. ( A , B ) TNF-α concentrations of brain extracts ( A ) and whole blood ( B ) of APPSWE-Tau mice, along with representative WT controls, were measured with a mouse SDC3 ELISA kit. Each group contained 8 animals. The bars represent the mean + SEM. Statistical significance vs. WT was assessed with ANOVA. ** p < 0.01. ( C ) Linear regression between the TNF-α content of blood and brain.
Techniques Used: Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: APPSWE-Tau mice exhibits increased SDC3 concentrations in the brain and the liver. ( A , B ) SDC3 concentrations of the brain ( A ) and liver ( C ) extracts of APPSWE-Tau mice and representative WT controls were measured with ELISA. Each group contained 8 animals. The bars represent the mean + SEM. Statistical significance vs. WT was assessed with ANOVA. * p < 0.05. ( C , D ) Linear regression between the TNF-α and SDC3 concentrations in the brain ( C ) and liver ( D ).
Techniques Used: Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: PBECs of APPSWE-Tau mice exhibits increased SDC3 expression. Isolated PBECs were treated with PECAM-1 and SDC3 antibodies, and SDC3 expression of PECAM-1 positive cells was analyzed with imaging flow cytometry. ( A ) Representative histogram showing the SDC3 expression of PBECs isolated from APPSWE-Tau and WT mice. ( B ) BF and fluorescent cellular images of PECAM-1 and SDC3 antibody-treated PBECs, isolated from APPSWE-Tau and WT mice. Each group contained eight animals. SDC3 expression of each sample was measured twice. ( C ) Detected fluorescence intensities were normalized to WT. The bars represent the mean + SEM. Statistical significance vs. WT was assessed with ANOVA. * p < 0.05. ( D ) Linear regression between in PBECs’ relative SDC3 expression and blood TNF-α concentrations.
Techniques Used: Expressing, Isolation, Imaging, Flow Cytometry, Fluorescence
Figure Legend Snippet: Monocytes isolated from APPSWE-Tau mice exhibits increased SDC3 expression. Isolated monocytes were treated with CD11b and SDC3 antibodies, and SDC3 expression of CD11b positive cells was analyzed with imaging flow cytometry. ( A ) Representative histogram showing the SDC3 expression of monocytes isolated from APPSWE-Tau and WT mice. ( B ) BF and fluorescent cellular images of CD11b and SDC3 antibody-treated monocytes, isolated from APPSWE-Tau and WT mice. Each group contained eight animals. SDC3 expression of each sample was measured twice. ( C ) Detected fluorescence intensities were normalized to WT. The bars represent the mean + SEM. Statistical significance vs. WT was assessed with ANOVA. *** p < 0.001. ( D ) Linear regression between the SDC3 expression of monocytes and Aβ plaque loads.
Techniques Used: Isolation, Expressing, Imaging, Flow Cytometry, Fluorescence