rat mmp 9 elisa kit picokine  (Boster Bio)


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    Boster Bio rat mmp 9 elisa kit picokine
    Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and <t>MMP-9</t> in serum. ( C ) MTT was conducted to test cell viability. *** P
    Rat Mmp 9 Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat mmp 9 elisa kit picokine/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat mmp 9 elisa kit picokine - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1"

    Article Title: Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.928480

    Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9 in serum. ( C ) MTT was conducted to test cell viability. *** P
    Figure Legend Snippet: Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9 in serum. ( C ) MTT was conducted to test cell viability. *** P

    Techniques Used: Multiple Displacement Amplification, Activity Assay, Transfection, Enzyme-linked Immunosorbent Assay, MTT Assay

    Induction of inflammatory factor secretion and oxidative stress by thrombin in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in the H/R model treated with thrombin, the H/R model treated with dabigatran, and the H/R model treated with a combination of thrombin and dabigatran, as well as the controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9. ( C ) MTT was conducted to test cell viability. *** P
    Figure Legend Snippet: Induction of inflammatory factor secretion and oxidative stress by thrombin in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in the H/R model treated with thrombin, the H/R model treated with dabigatran, and the H/R model treated with a combination of thrombin and dabigatran, as well as the controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9. ( C ) MTT was conducted to test cell viability. *** P

    Techniques Used: Multiple Displacement Amplification, Activity Assay, Enzyme-linked Immunosorbent Assay, MTT Assay

    2) Product Images from "Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes"

    Article Title: Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes

    Journal: Cells

    doi: 10.3390/cells7080096

    Effects of 2-chloroethanol (2-CE) on matrix metalloproteinases-9 (MMP-9) expression in astrocytes. ( A , B ) Astrocytes were treated with 30 mM 2-CE for the indicated times; ( C – E ) Astrocytes were treated with 0, 7.5, 15, and 30 mM 2-CE for 12 h. ( A , C ) MMP-9 protein levels in astrocytes measured by Western blotting; ( B , D ) MMP-9 protein levels in the culture media measured by ELISA; ( E ) MMP-9 mRNA levels in astrocytes analyzed by real-time (RT)-PCR. ( n = 4; mean ± SD; * p
    Figure Legend Snippet: Effects of 2-chloroethanol (2-CE) on matrix metalloproteinases-9 (MMP-9) expression in astrocytes. ( A , B ) Astrocytes were treated with 30 mM 2-CE for the indicated times; ( C – E ) Astrocytes were treated with 0, 7.5, 15, and 30 mM 2-CE for 12 h. ( A , C ) MMP-9 protein levels in astrocytes measured by Western blotting; ( B , D ) MMP-9 protein levels in the culture media measured by ELISA; ( E ) MMP-9 mRNA levels in astrocytes analyzed by real-time (RT)-PCR. ( n = 4; mean ± SD; * p

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

    3) Product Images from "microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury, et al. microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury"

    Article Title: microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury, et al. microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury

    Journal: Journal of Neurochemistry

    doi: 10.1111/jnc.14963

    miRNA‐9‐5p mimic inhibited NF‐κB/MMP‐9 pathway. (a) The immunoblotting and quantitative data of (b) NF‐κB and (C)MMP‐9 in BMECs treated with GANT61. (** p
    Figure Legend Snippet: miRNA‐9‐5p mimic inhibited NF‐κB/MMP‐9 pathway. (a) The immunoblotting and quantitative data of (b) NF‐κB and (C)MMP‐9 in BMECs treated with GANT61. (** p

    Techniques Used:

    Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation in vitro. (a) The immunoblotting and quantitative data of (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase‐3 acquired from BMECs. The level of (e) IL‐1β, (f) IL‐6, and (g) TNF‐α and (h) MMP‐9 was detected by ELISA in cell medium ( n = 4/group). (*** p
    Figure Legend Snippet: Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation in vitro. (a) The immunoblotting and quantitative data of (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase‐3 acquired from BMECs. The level of (e) IL‐1β, (f) IL‐6, and (g) TNF‐α and (h) MMP‐9 was detected by ELISA in cell medium ( n = 4/group). (*** p

    Techniques Used: Over Expression, In Vitro, Enzyme-linked Immunosorbent Assay

    Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation after TBI. (a) The immunoblotting and quantitative data of apoptosis‐related molecules (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase 3 acquired from the traumatic foci at 3‐day post‐CCI ( n = 5/group). The level of (e) MMP‐9 and inflammatory cytokines including (f) IL‐1β, (g) IL‐6, and (h) TNF‐α was detected by ELISA at 3‐day post‐CCI ( n = 5/group). (*** p
    Figure Legend Snippet: Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation after TBI. (a) The immunoblotting and quantitative data of apoptosis‐related molecules (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase 3 acquired from the traumatic foci at 3‐day post‐CCI ( n = 5/group). The level of (e) MMP‐9 and inflammatory cytokines including (f) IL‐1β, (g) IL‐6, and (h) TNF‐α was detected by ELISA at 3‐day post‐CCI ( n = 5/group). (*** p

    Techniques Used: Over Expression, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer"

    Article Title: Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer

    Journal: Oncology Letters

    doi: 10.3892/ol.2020.11508

    Effects of the combination of Pem and 125 I on the proliferation and mobility in NSCLC cells. (A) The mRNA expression of PD1L1 in four cell lines examined using reverse transcription-quantitative PCR. (B) The PD1L1 mRNA and protein levels in fresh human NSCLC tissues. (C) H460 (left) and A549 (right) cells were treated with DMSO, and Pem and 125 I, singularly or in combination for 24 h and the cell growth was measured using MTT assay. Amount of MMP2 (left) and MMP9 (right) released after (D) H460 and (E) A549 cells were incubated with DMSO, and Pem and 125 I, singularly or in combination for 8 h was determined using ELISA. Pem, pembroliumab; NSCLC, non-small cell lung cancer; PD1L1, programmed cell death 1 ligand 1; cont, control; MMP; matrix metalloproteinase; I, iodine.
    Figure Legend Snippet: Effects of the combination of Pem and 125 I on the proliferation and mobility in NSCLC cells. (A) The mRNA expression of PD1L1 in four cell lines examined using reverse transcription-quantitative PCR. (B) The PD1L1 mRNA and protein levels in fresh human NSCLC tissues. (C) H460 (left) and A549 (right) cells were treated with DMSO, and Pem and 125 I, singularly or in combination for 24 h and the cell growth was measured using MTT assay. Amount of MMP2 (left) and MMP9 (right) released after (D) H460 and (E) A549 cells were incubated with DMSO, and Pem and 125 I, singularly or in combination for 8 h was determined using ELISA. Pem, pembroliumab; NSCLC, non-small cell lung cancer; PD1L1, programmed cell death 1 ligand 1; cont, control; MMP; matrix metalloproteinase; I, iodine.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, MTT Assay, Incubation, Enzyme-linked Immunosorbent Assay

    5) Product Images from "Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes"

    Article Title: Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes

    Journal: Cells

    doi: 10.3390/cells7080096

    Effects of 2-chloroethanol (2-CE) on matrix metalloproteinases-9 (MMP-9) expression in astrocytes. ( A , B ) Astrocytes were treated with 30 mM 2-CE for the indicated times; ( C – E ) Astrocytes were treated with 0, 7.5, 15, and 30 mM 2-CE for 12 h. ( A , C ) MMP-9 protein levels in astrocytes measured by Western blotting; ( B , D ) MMP-9 protein levels in the culture media measured by ELISA; ( E ) MMP-9 mRNA levels in astrocytes analyzed by real-time (RT)-PCR. ( n = 4; mean ± SD; * p
    Figure Legend Snippet: Effects of 2-chloroethanol (2-CE) on matrix metalloproteinases-9 (MMP-9) expression in astrocytes. ( A , B ) Astrocytes were treated with 30 mM 2-CE for the indicated times; ( C – E ) Astrocytes were treated with 0, 7.5, 15, and 30 mM 2-CE for 12 h. ( A , C ) MMP-9 protein levels in astrocytes measured by Western blotting; ( B , D ) MMP-9 protein levels in the culture media measured by ELISA; ( E ) MMP-9 mRNA levels in astrocytes analyzed by real-time (RT)-PCR. ( n = 4; mean ± SD; * p

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

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    Boster Bio rat mmp 9 elisa kit picokine
    Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and <t>MMP-9</t> in serum. ( C ) MTT was conducted to test cell viability. *** P
    Rat Mmp 9 Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat mmp 9 elisa kit picokine/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat mmp 9 elisa kit picokine - by Bioz Stars, 2022-05
    93/100 stars
      Buy from Supplier

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    Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9 in serum. ( C ) MTT was conducted to test cell viability. *** P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1

    doi: 10.12659/MSM.928480

    Figure Lengend Snippet: Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9 in serum. ( C ) MTT was conducted to test cell viability. *** P

    Article Snippet: The ELISA kits used were as follows: Rat IL-1 beta ELISA Kit (ab255730), Rat IL-6 ELISA Kit (ab234570), Rat TNF alpha ELISA Kit (ab236712), Rat ICAM 1 ELISA Kit (ab100763) (all purchased from Abcam), and Rat MMP9 ELISA Kit (EK1463, Boster Biological Technology Co., Ltd).

    Techniques: Multiple Displacement Amplification, Activity Assay, Transfection, Enzyme-linked Immunosorbent Assay, MTT Assay

    Induction of inflammatory factor secretion and oxidative stress by thrombin in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in the H/R model treated with thrombin, the H/R model treated with dabigatran, and the H/R model treated with a combination of thrombin and dabigatran, as well as the controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9. ( C ) MTT was conducted to test cell viability. *** P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1

    doi: 10.12659/MSM.928480

    Figure Lengend Snippet: Induction of inflammatory factor secretion and oxidative stress by thrombin in H/R-injured cardiomyocytes. ( A ) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in the H/R model treated with thrombin, the H/R model treated with dabigatran, and the H/R model treated with a combination of thrombin and dabigatran, as well as the controls. ( B ) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9. ( C ) MTT was conducted to test cell viability. *** P

    Article Snippet: The ELISA kits used were as follows: Rat IL-1 beta ELISA Kit (ab255730), Rat IL-6 ELISA Kit (ab234570), Rat TNF alpha ELISA Kit (ab236712), Rat ICAM 1 ELISA Kit (ab100763) (all purchased from Abcam), and Rat MMP9 ELISA Kit (EK1463, Boster Biological Technology Co., Ltd).

    Techniques: Multiple Displacement Amplification, Activity Assay, Enzyme-linked Immunosorbent Assay, MTT Assay

    Effects of 2-chloroethanol (2-CE) on matrix metalloproteinases-9 (MMP-9) expression in astrocytes. ( A , B ) Astrocytes were treated with 30 mM 2-CE for the indicated times; ( C – E ) Astrocytes were treated with 0, 7.5, 15, and 30 mM 2-CE for 12 h. ( A , C ) MMP-9 protein levels in astrocytes measured by Western blotting; ( B , D ) MMP-9 protein levels in the culture media measured by ELISA; ( E ) MMP-9 mRNA levels in astrocytes analyzed by real-time (RT)-PCR. ( n = 4; mean ± SD; * p

    Journal: Cells

    Article Title: Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes

    doi: 10.3390/cells7080096

    Figure Lengend Snippet: Effects of 2-chloroethanol (2-CE) on matrix metalloproteinases-9 (MMP-9) expression in astrocytes. ( A , B ) Astrocytes were treated with 30 mM 2-CE for the indicated times; ( C – E ) Astrocytes were treated with 0, 7.5, 15, and 30 mM 2-CE for 12 h. ( A , C ) MMP-9 protein levels in astrocytes measured by Western blotting; ( B , D ) MMP-9 protein levels in the culture media measured by ELISA; ( E ) MMP-9 mRNA levels in astrocytes analyzed by real-time (RT)-PCR. ( n = 4; mean ± SD; * p

    Article Snippet: Secreted MMP-9 levels in the supernatants were measured using the rat total MMP-9 ELISA Kit (Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) according to the manufacturer’s instructions.

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

    miRNA‐9‐5p mimic inhibited NF‐κB/MMP‐9 pathway. (a) The immunoblotting and quantitative data of (b) NF‐κB and (C)MMP‐9 in BMECs treated with GANT61. (** p

    Journal: Journal of Neurochemistry

    Article Title: microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury, et al. microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury

    doi: 10.1111/jnc.14963

    Figure Lengend Snippet: miRNA‐9‐5p mimic inhibited NF‐κB/MMP‐9 pathway. (a) The immunoblotting and quantitative data of (b) NF‐κB and (C)MMP‐9 in BMECs treated with GANT61. (** p

    Article Snippet: 2.17 Enzyme‐linked immunosorbent assayThe contents of IL‐1β (RD, Cat# PRLB00), IL‐6 (RD, Cat# PR6000B), TNF‐α (RD, Cat# PRTA00), and MMP‐9 (BOSTER, Cat# EK1463) in different cell culture supernatants were tested by enzyme‐linked immunosorbent assay (ELISA) according to the manufacturer's instructions.

    Techniques:

    Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation in vitro. (a) The immunoblotting and quantitative data of (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase‐3 acquired from BMECs. The level of (e) IL‐1β, (f) IL‐6, and (g) TNF‐α and (h) MMP‐9 was detected by ELISA in cell medium ( n = 4/group). (*** p

    Journal: Journal of Neurochemistry

    Article Title: microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury, et al. microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury

    doi: 10.1111/jnc.14963

    Figure Lengend Snippet: Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation in vitro. (a) The immunoblotting and quantitative data of (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase‐3 acquired from BMECs. The level of (e) IL‐1β, (f) IL‐6, and (g) TNF‐α and (h) MMP‐9 was detected by ELISA in cell medium ( n = 4/group). (*** p

    Article Snippet: 2.17 Enzyme‐linked immunosorbent assayThe contents of IL‐1β (RD, Cat# PRLB00), IL‐6 (RD, Cat# PR6000B), TNF‐α (RD, Cat# PRTA00), and MMP‐9 (BOSTER, Cat# EK1463) in different cell culture supernatants were tested by enzyme‐linked immunosorbent assay (ELISA) according to the manufacturer's instructions.

    Techniques: Over Expression, In Vitro, Enzyme-linked Immunosorbent Assay

    Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation after TBI. (a) The immunoblotting and quantitative data of apoptosis‐related molecules (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase 3 acquired from the traumatic foci at 3‐day post‐CCI ( n = 5/group). The level of (e) MMP‐9 and inflammatory cytokines including (f) IL‐1β, (g) IL‐6, and (h) TNF‐α was detected by ELISA at 3‐day post‐CCI ( n = 5/group). (*** p

    Journal: Journal of Neurochemistry

    Article Title: microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury, et al. microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury

    doi: 10.1111/jnc.14963

    Figure Lengend Snippet: Over‐expression of miRNA‐9‐5p inhibited cellular apoptosis and inflammation after TBI. (a) The immunoblotting and quantitative data of apoptosis‐related molecules (b) Bcl‐2, (c) Bax, and (d) cleaved Caspase 3 acquired from the traumatic foci at 3‐day post‐CCI ( n = 5/group). The level of (e) MMP‐9 and inflammatory cytokines including (f) IL‐1β, (g) IL‐6, and (h) TNF‐α was detected by ELISA at 3‐day post‐CCI ( n = 5/group). (*** p

    Article Snippet: 2.17 Enzyme‐linked immunosorbent assayThe contents of IL‐1β (RD, Cat# PRLB00), IL‐6 (RD, Cat# PR6000B), TNF‐α (RD, Cat# PRTA00), and MMP‐9 (BOSTER, Cat# EK1463) in different cell culture supernatants were tested by enzyme‐linked immunosorbent assay (ELISA) according to the manufacturer's instructions.

    Techniques: Over Expression, Enzyme-linked Immunosorbent Assay

    Effects of the combination of Pem and 125 I on the proliferation and mobility in NSCLC cells. (A) The mRNA expression of PD1L1 in four cell lines examined using reverse transcription-quantitative PCR. (B) The PD1L1 mRNA and protein levels in fresh human NSCLC tissues. (C) H460 (left) and A549 (right) cells were treated with DMSO, and Pem and 125 I, singularly or in combination for 24 h and the cell growth was measured using MTT assay. Amount of MMP2 (left) and MMP9 (right) released after (D) H460 and (E) A549 cells were incubated with DMSO, and Pem and 125 I, singularly or in combination for 8 h was determined using ELISA. Pem, pembroliumab; NSCLC, non-small cell lung cancer; PD1L1, programmed cell death 1 ligand 1; cont, control; MMP; matrix metalloproteinase; I, iodine.

    Journal: Oncology Letters

    Article Title: Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer

    doi: 10.3892/ol.2020.11508

    Figure Lengend Snippet: Effects of the combination of Pem and 125 I on the proliferation and mobility in NSCLC cells. (A) The mRNA expression of PD1L1 in four cell lines examined using reverse transcription-quantitative PCR. (B) The PD1L1 mRNA and protein levels in fresh human NSCLC tissues. (C) H460 (left) and A549 (right) cells were treated with DMSO, and Pem and 125 I, singularly or in combination for 24 h and the cell growth was measured using MTT assay. Amount of MMP2 (left) and MMP9 (right) released after (D) H460 and (E) A549 cells were incubated with DMSO, and Pem and 125 I, singularly or in combination for 8 h was determined using ELISA. Pem, pembroliumab; NSCLC, non-small cell lung cancer; PD1L1, programmed cell death 1 ligand 1; cont, control; MMP; matrix metalloproteinase; I, iodine.

    Article Snippet: ELISA was performed using the matrix metalloproteinase (MMP)2 and MMP9 ELISA kits (Boster Biology Technnology; cat. nos.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, MTT Assay, Incubation, Enzyme-linked Immunosorbent Assay