hepatocyte growth factor hgf elisa kit  (Boster Bio)


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    Name:
    Rat HGF ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK1301
    Price:
    369.0
    Category:
    ELISA Kits
    Reactivity:
    Rat
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: NSO; Immunogen sequence: Q33-R495(alpha) & V496-L728(beta)
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    Structured Review

    Boster Bio hepatocyte growth factor hgf elisa kit
    Rat HGF ELISA Kit PicoKine

    https://www.bioz.com/result/hepatocyte growth factor hgf elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf elisa kit - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing"

    Article Title: c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing

    Journal: Cell Transplantation

    doi: 10.1177/0963689721989605

    The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, HGF, and bFGF level in the conditioned media were assessed by ELISA assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P
    Figure Legend Snippet: The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, HGF, and bFGF level in the conditioned media were assessed by ELISA assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P

    Techniques Used: Migration, Activity Assay, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Staining

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing
    Article Snippet: Enzyme Linked Immunosorbent Assay (ELISA) AnalysisThe different groups of BM-MSCs 24 h conditioned culture medium was collected and then centrifuged at 1,000 rpm/min for 5 min. .. The supernatant was absorbed and the amount of angiogenic factors was analyzed by vascular endothelial growth factor A (VEGFA) ELISA kit (BOSTER Biological Technology, Wuhan, China), basic fibroblast growth factor (bFGF) ELISA kit (Cusabio Biotech, Wuhan, China), and hepatocyte growth factor (HGF) ELISA kit (BOSTER Biological Technology, Wuhan, China) according to the manufacturer’s instructions. .. Cell Counting Kit-8 AssayThe Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotechnology, Shanghai, China) was used to detect the cellular proliferation of BM-MSCs from different groups according to the manufacturer’s protocols.

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  • 86
    Boster Bio rat hgf elisa kit picokine
    Effects of OA on the expression of <t>HGF</t> in A7r5 cells. (A) <t>ELISA</t> results at 24 h identified that low concentrations of OA (50 µmol/l) upregulated the level of HGF, but HGF was downregulated at high concentrations (800 µmol/l) of OA. (B) Western blot analysis at 24 h demonstrated that low concentrations of OA (50 µmol/l) increased the expression of HGF, but high concentrations of OA (800 µmol/l) downregulated this expression. (C) Western blot analysis showed that the increased HGF expression at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). (D) Reverse transcription-quantitative PCR analysis at 24 h indicated that the increased expression of HGF at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). Data are presented as the mean ± SD (n=3). **P
    Rat Hgf Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat hgf elisa kit picokine/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat hgf elisa kit picokine - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    94
    Boster Bio hepatocyte growth factor hgf elisa kit
    The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, <t>HGF,</t> and bFGF level in the conditioned media were assessed by <t>ELISA</t> assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P
    Hepatocyte Growth Factor Hgf Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf elisa kit - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of OA on the expression of HGF in A7r5 cells. (A) ELISA results at 24 h identified that low concentrations of OA (50 µmol/l) upregulated the level of HGF, but HGF was downregulated at high concentrations (800 µmol/l) of OA. (B) Western blot analysis at 24 h demonstrated that low concentrations of OA (50 µmol/l) increased the expression of HGF, but high concentrations of OA (800 µmol/l) downregulated this expression. (C) Western blot analysis showed that the increased HGF expression at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). (D) Reverse transcription-quantitative PCR analysis at 24 h indicated that the increased expression of HGF at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). Data are presented as the mean ± SD (n=3). **P

    Journal: Molecular Medicine Reports

    Article Title: Oleic acid induces A7r5 cell proliferation and migration associated with increased expression of HGF and p-p38

    doi: 10.3892/mmr.2021.12123

    Figure Lengend Snippet: Effects of OA on the expression of HGF in A7r5 cells. (A) ELISA results at 24 h identified that low concentrations of OA (50 µmol/l) upregulated the level of HGF, but HGF was downregulated at high concentrations (800 µmol/l) of OA. (B) Western blot analysis at 24 h demonstrated that low concentrations of OA (50 µmol/l) increased the expression of HGF, but high concentrations of OA (800 µmol/l) downregulated this expression. (C) Western blot analysis showed that the increased HGF expression at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). (D) Reverse transcription-quantitative PCR analysis at 24 h indicated that the increased expression of HGF at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). Data are presented as the mean ± SD (n=3). **P

    Article Snippet: Commercial HGF ELISA kit (cat. no. EK1301) was purchased from Boster Biological Technology.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Real-time Polymerase Chain Reaction

    The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, HGF, and bFGF level in the conditioned media were assessed by ELISA assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P

    Journal: Cell Transplantation

    Article Title: c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing

    doi: 10.1177/0963689721989605

    Figure Lengend Snippet: The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, HGF, and bFGF level in the conditioned media were assessed by ELISA assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P

    Article Snippet: The supernatant was absorbed and the amount of angiogenic factors was analyzed by vascular endothelial growth factor A (VEGFA) ELISA kit (BOSTER Biological Technology, Wuhan, China), basic fibroblast growth factor (bFGF) ELISA kit (Cusabio Biotech, Wuhan, China), and hepatocyte growth factor (HGF) ELISA kit (BOSTER Biological Technology, Wuhan, China) according to the manufacturer’s instructions.

    Techniques: Migration, Activity Assay, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Staining