cd320 expression  (Boster Bio)


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    Name:
    Human CD320 8D6A TCblR ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK1255
    Price:
    369.0
    Category:
    ELISA Kits
    Reactivity:
    Human
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: NSO; Immunogen sequence: S36-Y229
    Buy from Supplier


    Structured Review

    Boster Bio cd320 expression
    Human CD320 8D6A TCblR ELISA Kit PicoKine

    https://www.bioz.com/result/cd320 expression/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd320 expression - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells"

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

    Journal: eLife

    doi: 10.7554/eLife.56059

    Structural alignments of the LDLR domains of human CD320. ( A ) The structure alignments of human CD320 LDLR1 (residues 54–89) (light blue) and LDLR2 (residues 132–166) (violet), and corin LDLR8 module (residues 655–689) (cyan) with the calcium ions (spheres) and side chains and disulfide bonds (sticks). ( B ) The DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules are enlarged from ( A ). ( C ) The local alignment of the DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules.
    Figure Legend Snippet: Structural alignments of the LDLR domains of human CD320. ( A ) The structure alignments of human CD320 LDLR1 (residues 54–89) (light blue) and LDLR2 (residues 132–166) (violet), and corin LDLR8 module (residues 655–689) (cyan) with the calcium ions (spheres) and side chains and disulfide bonds (sticks). ( B ) The DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules are enlarged from ( A ). ( C ) The local alignment of the DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules.

    Techniques Used:

    Effects of Rab11b knockdown on corin and CD320 expression in MDCK cells. MDCK cells were transfected with non-targeting (shNC) or two sets of RAB11B -targeting (shRab11b1 and shRab11b2) shRNAs. Quantitative RT-PCR ( A ) and western blotting ( B ) were used to examine Rab11b mRNA and protein expression, respectively, in the shRNA-transfected MDCK cells. ( C ) Apical corin (top panels) and CD320 (lower panels) expression in the shRNA-transfected MDCK cells was examined by immunostaining and confocal microscopy with X-Y and X-Z views indicated. ( D ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Key Resources Table. Information on genes, cell lines, plasmids and antibodies. Source data for Figure 8—figure supplement 2A . Source data for Figure 8—figure supplement 2D .
    Figure Legend Snippet: Effects of Rab11b knockdown on corin and CD320 expression in MDCK cells. MDCK cells were transfected with non-targeting (shNC) or two sets of RAB11B -targeting (shRab11b1 and shRab11b2) shRNAs. Quantitative RT-PCR ( A ) and western blotting ( B ) were used to examine Rab11b mRNA and protein expression, respectively, in the shRNA-transfected MDCK cells. ( C ) Apical corin (top panels) and CD320 (lower panels) expression in the shRNA-transfected MDCK cells was examined by immunostaining and confocal microscopy with X-Y and X-Z views indicated. ( D ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Key Resources Table. Information on genes, cell lines, plasmids and antibodies. Source data for Figure 8—figure supplement 2A . Source data for Figure 8—figure supplement 2D .

    Techniques Used: Expressing, Transfection, Quantitative RT-PCR, Western Blot, shRNA, Immunostaining, Confocal Microscopy

    Effects of Rab11a inhibition on apical corin and CD320 expression. ( A ) Corin WT co-expressed with Rab11a, Rab11b, or dominant-negative (DN) Rab11a or Rab11b mutants in MDCK cells was analyzed by immunostaining and confocal microscopy. Quantitative data of the ratio of F BL /F Total from four experiments are shown. ( B ) Western blotting of Rab11a protein in MDCK cells transfected with non-targeting (shNC) and two sets of RAB11A -targeting (shRab11a1 and shRab11a2) shRNAs. GAPDH was used as a control. ( C ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Source data for Figure 8—figure supplement 1A . Source data for Figure 8—figure supplement 1C .
    Figure Legend Snippet: Effects of Rab11a inhibition on apical corin and CD320 expression. ( A ) Corin WT co-expressed with Rab11a, Rab11b, or dominant-negative (DN) Rab11a or Rab11b mutants in MDCK cells was analyzed by immunostaining and confocal microscopy. Quantitative data of the ratio of F BL /F Total from four experiments are shown. ( B ) Western blotting of Rab11a protein in MDCK cells transfected with non-targeting (shNC) and two sets of RAB11A -targeting (shRab11a1 and shRab11a2) shRNAs. GAPDH was used as a control. ( C ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Source data for Figure 8—figure supplement 1A . Source data for Figure 8—figure supplement 1C .

    Techniques Used: Inhibition, Expressing, Dominant Negative Mutation, Immunostaining, Confocal Microscopy, Western Blot, Transfection, shRNA

    Quantitative analysis of CD320 and corin expression in MDCK cells. ( A and B ) CD320 WT and mutant expression in MDCK cells was examined by immunostaining and confocal microscopy. Fluorescent signals along the X-Z axis were analyzed with Image J software to calculate the ratio of F BL /F Total . The data are mean ± SEM (n = 5). Source data for Figure 7—figure supplement 1A . Source data for Figure 7—figure supplement 1B .
    Figure Legend Snippet: Quantitative analysis of CD320 and corin expression in MDCK cells. ( A and B ) CD320 WT and mutant expression in MDCK cells was examined by immunostaining and confocal microscopy. Fluorescent signals along the X-Z axis were analyzed with Image J software to calculate the ratio of F BL /F Total . The data are mean ± SEM (n = 5). Source data for Figure 7—figure supplement 1A . Source data for Figure 7—figure supplement 1B .

    Techniques Used: Expressing, Mutagenesis, Immunostaining, Confocal Microscopy, Software

    Related Articles

    Immunostaining:

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells
    Article Snippet: .. Immunostaining in tissue sectionsTo examine corin and CD320 expression and subcellular localization in renal tubular epithelial cells, kidney tissues were fixed in 4% (v/v) formaldehyde, embedded in paraffin, cut in 3 μm sections, and mounted on chrome alum gelatin-coated slides (Aladdin, p1650). .. After dewaxing, sections were boiled in sodium citrate buffer (0.01 mM, pH 6.0) for 1 min for antigen retrieval, and soaked in a methanol solution containing 3% (v/v) hydrogen peroxide for 5 min at room temperature to block endogenous peroxidase activity.

    Expressing:

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells
    Article Snippet: .. Immunostaining in tissue sectionsTo examine corin and CD320 expression and subcellular localization in renal tubular epithelial cells, kidney tissues were fixed in 4% (v/v) formaldehyde, embedded in paraffin, cut in 3 μm sections, and mounted on chrome alum gelatin-coated slides (Aladdin, p1650). .. After dewaxing, sections were boiled in sodium citrate buffer (0.01 mM, pH 6.0) for 1 min for antigen retrieval, and soaked in a methanol solution containing 3% (v/v) hydrogen peroxide for 5 min at room temperature to block endogenous peroxidase activity.

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    Boster Bio cd320 expression
    Structural alignments of the LDLR domains of human <t>CD320.</t> ( A ) The structure alignments of human CD320 LDLR1 (residues 54–89) (light blue) and LDLR2 (residues 132–166) (violet), and corin LDLR8 module (residues 655–689) (cyan) with the calcium ions (spheres) and side chains and disulfide bonds (sticks). ( B ) The DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules are enlarged from ( A ). ( C ) The local alignment of the DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules.
    Cd320 Expression, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd320 expression/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd320 expression - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Structural alignments of the LDLR domains of human CD320. ( A ) The structure alignments of human CD320 LDLR1 (residues 54–89) (light blue) and LDLR2 (residues 132–166) (violet), and corin LDLR8 module (residues 655–689) (cyan) with the calcium ions (spheres) and side chains and disulfide bonds (sticks). ( B ) The DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules are enlarged from ( A ). ( C ) The local alignment of the DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules.

    Journal: eLife

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

    doi: 10.7554/eLife.56059

    Figure Lengend Snippet: Structural alignments of the LDLR domains of human CD320. ( A ) The structure alignments of human CD320 LDLR1 (residues 54–89) (light blue) and LDLR2 (residues 132–166) (violet), and corin LDLR8 module (residues 655–689) (cyan) with the calcium ions (spheres) and side chains and disulfide bonds (sticks). ( B ) The DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules are enlarged from ( A ). ( C ) The local alignment of the DSSDE motifs in CD320 LDLR2 and corin LDLR8 modules.

    Article Snippet: Immunostaining in tissue sectionsTo examine corin and CD320 expression and subcellular localization in renal tubular epithelial cells, kidney tissues were fixed in 4% (v/v) formaldehyde, embedded in paraffin, cut in 3 μm sections, and mounted on chrome alum gelatin-coated slides (Aladdin, p1650).

    Techniques:

    Effects of Rab11b knockdown on corin and CD320 expression in MDCK cells. MDCK cells were transfected with non-targeting (shNC) or two sets of RAB11B -targeting (shRab11b1 and shRab11b2) shRNAs. Quantitative RT-PCR ( A ) and western blotting ( B ) were used to examine Rab11b mRNA and protein expression, respectively, in the shRNA-transfected MDCK cells. ( C ) Apical corin (top panels) and CD320 (lower panels) expression in the shRNA-transfected MDCK cells was examined by immunostaining and confocal microscopy with X-Y and X-Z views indicated. ( D ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Key Resources Table. Information on genes, cell lines, plasmids and antibodies. Source data for Figure 8—figure supplement 2A . Source data for Figure 8—figure supplement 2D .

    Journal: eLife

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

    doi: 10.7554/eLife.56059

    Figure Lengend Snippet: Effects of Rab11b knockdown on corin and CD320 expression in MDCK cells. MDCK cells were transfected with non-targeting (shNC) or two sets of RAB11B -targeting (shRab11b1 and shRab11b2) shRNAs. Quantitative RT-PCR ( A ) and western blotting ( B ) were used to examine Rab11b mRNA and protein expression, respectively, in the shRNA-transfected MDCK cells. ( C ) Apical corin (top panels) and CD320 (lower panels) expression in the shRNA-transfected MDCK cells was examined by immunostaining and confocal microscopy with X-Y and X-Z views indicated. ( D ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Key Resources Table. Information on genes, cell lines, plasmids and antibodies. Source data for Figure 8—figure supplement 2A . Source data for Figure 8—figure supplement 2D .

    Article Snippet: Immunostaining in tissue sectionsTo examine corin and CD320 expression and subcellular localization in renal tubular epithelial cells, kidney tissues were fixed in 4% (v/v) formaldehyde, embedded in paraffin, cut in 3 μm sections, and mounted on chrome alum gelatin-coated slides (Aladdin, p1650).

    Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, shRNA, Immunostaining, Confocal Microscopy

    Effects of Rab11a inhibition on apical corin and CD320 expression. ( A ) Corin WT co-expressed with Rab11a, Rab11b, or dominant-negative (DN) Rab11a or Rab11b mutants in MDCK cells was analyzed by immunostaining and confocal microscopy. Quantitative data of the ratio of F BL /F Total from four experiments are shown. ( B ) Western blotting of Rab11a protein in MDCK cells transfected with non-targeting (shNC) and two sets of RAB11A -targeting (shRab11a1 and shRab11a2) shRNAs. GAPDH was used as a control. ( C ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Source data for Figure 8—figure supplement 1A . Source data for Figure 8—figure supplement 1C .

    Journal: eLife

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

    doi: 10.7554/eLife.56059

    Figure Lengend Snippet: Effects of Rab11a inhibition on apical corin and CD320 expression. ( A ) Corin WT co-expressed with Rab11a, Rab11b, or dominant-negative (DN) Rab11a or Rab11b mutants in MDCK cells was analyzed by immunostaining and confocal microscopy. Quantitative data of the ratio of F BL /F Total from four experiments are shown. ( B ) Western blotting of Rab11a protein in MDCK cells transfected with non-targeting (shNC) and two sets of RAB11A -targeting (shRab11a1 and shRab11a2) shRNAs. GAPDH was used as a control. ( C ) Quantitative data of the ratio of F BL /F Total for corin and CD320 expression on apical and basolateral membranes in shRNA-transfected MDCK cells from four experiments. Statistical analysis was done with ANOVA. n.s., not significant vs . respective controls in each experiment. Source data for Figure 8—figure supplement 1A . Source data for Figure 8—figure supplement 1C .

    Article Snippet: Immunostaining in tissue sectionsTo examine corin and CD320 expression and subcellular localization in renal tubular epithelial cells, kidney tissues were fixed in 4% (v/v) formaldehyde, embedded in paraffin, cut in 3 μm sections, and mounted on chrome alum gelatin-coated slides (Aladdin, p1650).

    Techniques: Inhibition, Expressing, Dominant Negative Mutation, Immunostaining, Confocal Microscopy, Western Blot, Transfection, shRNA

    Quantitative analysis of CD320 and corin expression in MDCK cells. ( A and B ) CD320 WT and mutant expression in MDCK cells was examined by immunostaining and confocal microscopy. Fluorescent signals along the X-Z axis were analyzed with Image J software to calculate the ratio of F BL /F Total . The data are mean ± SEM (n = 5). Source data for Figure 7—figure supplement 1A . Source data for Figure 7—figure supplement 1B .

    Journal: eLife

    Article Title: A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

    doi: 10.7554/eLife.56059

    Figure Lengend Snippet: Quantitative analysis of CD320 and corin expression in MDCK cells. ( A and B ) CD320 WT and mutant expression in MDCK cells was examined by immunostaining and confocal microscopy. Fluorescent signals along the X-Z axis were analyzed with Image J software to calculate the ratio of F BL /F Total . The data are mean ± SEM (n = 5). Source data for Figure 7—figure supplement 1A . Source data for Figure 7—figure supplement 1B .

    Article Snippet: Immunostaining in tissue sectionsTo examine corin and CD320 expression and subcellular localization in renal tubular epithelial cells, kidney tissues were fixed in 4% (v/v) formaldehyde, embedded in paraffin, cut in 3 μm sections, and mounted on chrome alum gelatin-coated slides (Aladdin, p1650).

    Techniques: Expressing, Mutagenesis, Immunostaining, Confocal Microscopy, Software