rat il 18 ek0592  (Boster Bio)


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    Boster Bio rat il 18 ek0592
    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma <t>IL-18</t> levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
    Rat Il 18 Ek0592, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93/100 stars

    Images

    1) Product Images from "Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs"

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    Journal: Respiratory Research

    doi: 10.1186/s12931-022-02279-0

    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
    Figure Legend Snippet: Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Techniques Used: Expressing, Two Tailed Test

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia
    Figure Legend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Techniques Used: Enzyme-linked Immunosorbent Assay

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)
    Figure Legend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Techniques Used: Enzyme-linked Immunosorbent Assay

    DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia
    Figure Legend Snippet: DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay

    rat il 18 ek0592  (Boster Bio)


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    Structured Review

    Boster Bio rat il 18 ek0592
    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma <t>IL-18</t> levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
    Rat Il 18 Ek0592, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat il 18 ek0592/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat il 18 ek0592 - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs"

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    Journal: Respiratory Research

    doi: 10.1186/s12931-022-02279-0

    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
    Figure Legend Snippet: Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Techniques Used: Expressing, Two Tailed Test

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia
    Figure Legend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Techniques Used: Enzyme-linked Immunosorbent Assay

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)
    Figure Legend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Techniques Used: Enzyme-linked Immunosorbent Assay

    DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia
    Figure Legend Snippet: DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay

    mouse il 18 ek0433  (Boster Bio)


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    Structured Review

    Boster Bio mouse il 18 ek0433
    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma <t>IL-18</t> levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
    Mouse Il 18 Ek0433, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 18 ek0433/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 18 ek0433 - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs"

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    Journal: Respiratory Research

    doi: 10.1186/s12931-022-02279-0

    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
    Figure Legend Snippet: Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Techniques Used: Expressing, Two Tailed Test

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia
    Figure Legend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Techniques Used: Enzyme-linked Immunosorbent Assay

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)
    Figure Legend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Techniques Used: Enzyme-linked Immunosorbent Assay

    DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia
    Figure Legend Snippet: DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay

    il 18 elisa kit  (Boster Bio)


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    Boster Bio il 18 elisa kit
    Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) <t>IL-18</t> uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).
    Il 18 Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 18 elisa kit - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period"

    Article Title: Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2017/3647080

    Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) IL-18 uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).
    Figure Legend Snippet: Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) IL-18 uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).

    Techniques Used: Expressing, In Vitro

    elisa kits  (Boster Bio)


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    Boster Bio elisa kits
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    immunosorbent assay elisa kits  (Boster Bio)


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    Boster Bio immunosorbent assay elisa kits
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    rat elisa kit  (Boster Bio)


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    Boster Bio rat elisa kit
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    elisa kit  (Boster Bio)


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    rat il 18 elisa kit  (Boster Bio)


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    Boster Bio rat il 18 elisa kit
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    elisa kit  (Boster Bio)


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    Boster Bio elisa kit
    a Comparison of expression and subcellular distribution of caspase-1 by double staining of caspase-1 (green) and CD31 (red) in atherosclerotic lesions of ApoE −/− mice among various groups: ND, ND + Ni (nicotine), HFD, or HFD + Ni. Note the presence of caspase-1 in endothelial cells as indicated by the co-localization of caspase-1 and CD31 (an endothelial marker). Magnification: ×200. b Identification of endothelial cell death by co-localized staining of CD31 (red) and TUNEL (green). The nuclei were stained blue with DAPI. Scale bar = 100 μm. Magnification: ×200. c Increases in the protein levels of caspase-1 by nicotine in the HFD group, as revealed by western blot analysis. d – h Increases in the expression of pyroptosis-related genes (NLRP3, ASC, <t>caspase-1,</t> <t>IL-1β,</t> and IL-18) at both protein and mRNA levels by nicotine in intimal samples of ApoE −/− mice of the HFD group. n = 6 mice for each group. The data are presented as the mean ± SEM., * P < 0.05. i – j Elevation of mean serum concentrations of IL-1β and IL-18 by nicotine in HFD-fed mice, as determined by <t>ELISA</t> assay. The data are shown as mean ± SEM. n = 6 mice in each group. * P < 0.05
    Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis"

    Article Title: Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-017-0257-3

    a Comparison of expression and subcellular distribution of caspase-1 by double staining of caspase-1 (green) and CD31 (red) in atherosclerotic lesions of ApoE −/− mice among various groups: ND, ND + Ni (nicotine), HFD, or HFD + Ni. Note the presence of caspase-1 in endothelial cells as indicated by the co-localization of caspase-1 and CD31 (an endothelial marker). Magnification: ×200. b Identification of endothelial cell death by co-localized staining of CD31 (red) and TUNEL (green). The nuclei were stained blue with DAPI. Scale bar = 100 μm. Magnification: ×200. c Increases in the protein levels of caspase-1 by nicotine in the HFD group, as revealed by western blot analysis. d – h Increases in the expression of pyroptosis-related genes (NLRP3, ASC, caspase-1, IL-1β, and IL-18) at both protein and mRNA levels by nicotine in intimal samples of ApoE −/− mice of the HFD group. n = 6 mice for each group. The data are presented as the mean ± SEM., * P < 0.05. i – j Elevation of mean serum concentrations of IL-1β and IL-18 by nicotine in HFD-fed mice, as determined by ELISA assay. The data are shown as mean ± SEM. n = 6 mice in each group. * P < 0.05
    Figure Legend Snippet: a Comparison of expression and subcellular distribution of caspase-1 by double staining of caspase-1 (green) and CD31 (red) in atherosclerotic lesions of ApoE −/− mice among various groups: ND, ND + Ni (nicotine), HFD, or HFD + Ni. Note the presence of caspase-1 in endothelial cells as indicated by the co-localization of caspase-1 and CD31 (an endothelial marker). Magnification: ×200. b Identification of endothelial cell death by co-localized staining of CD31 (red) and TUNEL (green). The nuclei were stained blue with DAPI. Scale bar = 100 μm. Magnification: ×200. c Increases in the protein levels of caspase-1 by nicotine in the HFD group, as revealed by western blot analysis. d – h Increases in the expression of pyroptosis-related genes (NLRP3, ASC, caspase-1, IL-1β, and IL-18) at both protein and mRNA levels by nicotine in intimal samples of ApoE −/− mice of the HFD group. n = 6 mice for each group. The data are presented as the mean ± SEM., * P < 0.05. i – j Elevation of mean serum concentrations of IL-1β and IL-18 by nicotine in HFD-fed mice, as determined by ELISA assay. The data are shown as mean ± SEM. n = 6 mice in each group. * P < 0.05

    Techniques Used: Expressing, Double Staining, Marker, Staining, TUNEL Assay, Western Blot, Enzyme-linked Immunosorbent Assay

    rat il 18 elisa kit  (Boster Bio)


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    Boster Bio rat il 18 elisa kit
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    • rat il 18 ek0592  (Boster Bio)
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    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma <t>IL-18</t> levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
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    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma <t>IL-18</t> levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control
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    Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) <t>IL-18</t> uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).
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    Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) <t>IL-18</t> uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).
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    Image Search Results


    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Expressing, Two Tailed Test

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Enzyme-linked Immunosorbent Assay

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Enzyme-linked Immunosorbent Assay

    DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Staining, Enzyme-linked Immunosorbent Assay

    Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: Patients with pulmonary hypertension (PH) have high expression levels of canonical caspase-1-dependent pyroptosis genes. A The gene expression levels of NLRP3 , IL18 and CASP1 in the lung tissue of patients with PH (n = 15) and control subjects (n = 11). The database is GSE113439. B The gene expression levels of ASC and GSDMD in the lung tissue of patients with PH (n = 18) and control subjects (n = 13). The database is GSE15197. C An unnormalized UMAP plot of GSE113439 and GSE15197. D A normalized UMAP plot of the GSE113439 and GSE15197. E An unnormalized expression density plot of GSE113439 and GSE15197. F A normalization expression density plot for GSE113439 and GSE15197. G Normalized expression distribution plots for GSE113439 and GSE15197. H GSEA of the pyroptosis gene set. Normalized enrichment score (NES) = 1.5578, nominal P value = 0.0301. I Plasma IL-1β levels in patients with PH increase. J Plasma IL-18 levels in patients with PH increase. Values are the mean ± S.E.M. and were analysed by the unpaired two-tailed Student’s t test. *P < 0.05 vs. the normal control

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Expressing, Two Tailed Test

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D , E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Enzyme-linked Immunosorbent Assay

    DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of hypoxia-induced PH rats. A Representative WB images and analysis of caspase-1 and CI. caspase-1. B Representative WB images and analysis of IL-18. C Representative WB images and analysis of IL-1β. D Representative WB images and analysis of GSDMD and N-GSDMD. E Representative WB images and analysis of NLRP3 and ASC. F , G ELISA detection of inflammatory factors (IL-18 and IL-1β). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia (n = 5 each)

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Enzyme-linked Immunosorbent Assay

    DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Journal: Respiratory Research

    Article Title: Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs

    doi: 10.1186/s12931-022-02279-0

    Figure Lengend Snippet: DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F , G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H , I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and # P < 0.05 vs. normoxia

    Article Snippet: ELISA kits for mouse IL-1β (RK00006), human IL-1β (RK00001), rat IL-1β (RK00009) and human IL-18 (RK00176) were obtained from ABclonal (Wuhan, China), and mouse IL-18 (EK0433) and rat IL-18 (EK0592) were obtained from BOSTER (Wuhan, China).

    Techniques: Staining, Enzyme-linked Immunosorbent Assay

    Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) IL-18 uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period

    doi: 10.1155/2017/3647080

    Figure Lengend Snippet: Expression of the levels of (a) VEGF, (b) sFlt-1, (c) IL-15, and (d) IL-18 uDCs secreting in vitro. ∗ or ∗∗ represents that there is significant difference between M and N groups ( P < 0.05 or P < 0.01). # or ## represents that there is significant difference compared with M group ( P < 0.05 or P < 0.01).

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) plates, cell strainers (aperture 70 μ m, nylon), antibody-coating buffer (Cat. number AR1106-4), VEGF (Cat. number EK0540, Lot. number 2541253523), ELISA kit, and IL-18 ELISA kit (Cat. number EK0592, Lot. number 2971251822) were purchased from Wuhan Boster Biological Engineering Co., Ltd., China.

    Techniques: Expressing, In Vitro