il 10  (Boster Bio)


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    Name:
    Rat IL 10 ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK0418
    Price:
    369.0
    Category:
    ELISA Kits
    Reactivity:
    Rat
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: E.coli; Immunogen sequence: S19-N178
    Buy from Supplier


    Structured Review

    Boster Bio il 10
    Rat IL 10 ELISA Kit PicoKine

    https://www.bioz.com/result/il 10/product/Boster Bio
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 10 - by Bioz Stars, 2021-06
    96/100 stars

    Images

    1) Product Images from "Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation"

    Article Title: Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation

    Journal: The Korean Journal of Pain

    doi: 10.3344/kjp.2021.34.1.47

    Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase IL-10 expression of rats with nucleus pulposus (NP) (** P
    Figure Legend Snippet: Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase IL-10 expression of rats with nucleus pulposus (NP) (** P

    Techniques Used: Expressing

    2) Product Images from "Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg"

    Article Title: Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2020/5375981

    The concentrations of cytokines in rats. (a) The concentration of IL-6, n = 8. (b) The concentration of IL-17, n = 8. (c) The concentration of TGF- β 1, n = 8. (d) The concentration of IL-10, n = 8. Data are expressed as the means ± SD values. ∗ P
    Figure Legend Snippet: The concentrations of cytokines in rats. (a) The concentration of IL-6, n = 8. (b) The concentration of IL-17, n = 8. (c) The concentration of TGF- β 1, n = 8. (d) The concentration of IL-10, n = 8. Data are expressed as the means ± SD values. ∗ P

    Techniques Used: Concentration Assay

    3) Product Images from "Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats"

    Article Title: Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S106912

    NiONPs induce caspase-1-dependent cytokine secretion in RAW264.7 cells. Notes: ( A – C ) The pro-caspase-1 and pro-IL-1β levels in cell lysates (input) and processed caspase-1 and IL-1β levels in culture SNs after treatment with different doses (0 µg/cm 2 , 1 µg/cm 2 , 2 µg/cm 2 , and 4 µg/cm 2 ) of NiONPs were analyzed by Western blotting. ( D ) Caspase-1 activity in RAW264.7 cells. ( E ) Detection of IL-1β expression in the SN by ELISA. * P
    Figure Legend Snippet: NiONPs induce caspase-1-dependent cytokine secretion in RAW264.7 cells. Notes: ( A – C ) The pro-caspase-1 and pro-IL-1β levels in cell lysates (input) and processed caspase-1 and IL-1β levels in culture SNs after treatment with different doses (0 µg/cm 2 , 1 µg/cm 2 , 2 µg/cm 2 , and 4 µg/cm 2 ) of NiONPs were analyzed by Western blotting. ( D ) Caspase-1 activity in RAW264.7 cells. ( E ) Detection of IL-1β expression in the SN by ELISA. * P

    Techniques Used: Western Blot, Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats"

    Article Title: Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2021.e06474

    Effect of DM and MLT on the serum levels of pro-inflammatory mediators. Tumor necrosis factor alpha (TNF-α) (a), interleukin 6 (IL-6) (b), interleukin 1 beta (IL-1β) (c) and anti-inflammatory cytokine interleukin 10 (IL-10) (d) of rats in the different groups. Values are expressed as means ± SEM; (n = 5). (∗, ∗∗ and ∗∗∗ indicate statistical significance at P
    Figure Legend Snippet: Effect of DM and MLT on the serum levels of pro-inflammatory mediators. Tumor necrosis factor alpha (TNF-α) (a), interleukin 6 (IL-6) (b), interleukin 1 beta (IL-1β) (c) and anti-inflammatory cytokine interleukin 10 (IL-10) (d) of rats in the different groups. Values are expressed as means ± SEM; (n = 5). (∗, ∗∗ and ∗∗∗ indicate statistical significance at P

    Techniques Used:

    5) Product Images from "Role of CCR7 on dendritic cell-mediated immune tolerance in the airways of allergy-induced asthmatic rats"

    Article Title: Role of CCR7 on dendritic cell-mediated immune tolerance in the airways of allergy-induced asthmatic rats

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2019.10694

    Protein expressions of (A) IFN-γ and IL-4, (B) IL-12 and IL-10, (C) TGF-β and (D) IgE in serum. Protein expression levels of various cytokines were assessed by ELISA. **P
    Figure Legend Snippet: Protein expressions of (A) IFN-γ and IL-4, (B) IL-12 and IL-10, (C) TGF-β and (D) IgE in serum. Protein expression levels of various cytokines were assessed by ELISA. **P

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    Protein expression levels of (A) IFN-γ and IL-4, (B) IL-12 and IL-10, (C) TGF-β and (D) IgE in bronchoalveolar lavage fluid. Protein expression levels of various cytokines were assessed by ELISA. **P
    Figure Legend Snippet: Protein expression levels of (A) IFN-γ and IL-4, (B) IL-12 and IL-10, (C) TGF-β and (D) IgE in bronchoalveolar lavage fluid. Protein expression levels of various cytokines were assessed by ELISA. **P

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    6) Product Images from "Cyanidin-3-O-β-glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF-β1/p38 MAPK/CREB signaling pathway"

    Article Title: Cyanidin-3-O-β-glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF-β1/p38 MAPK/CREB signaling pathway

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2021.11977

    Effect of Cy-3-g on inflammatory responses and oxidative stress in rats with pulmonary artery hypertension. Rats were randomly assigned into four groups (n=15): Control group, model group (MCT), Cy-3-g-L group and Cy-3-g-H group. On the first day of the experiments, the rats in the MCT, Cy-3-g-L and Cy-3-g-H groups were injected with MCT (60 mg/kg). On days 2–28, the rats in the Cy-3-g-L and Cy-3-g-H groups received a gavage of 200 and 400 mg/kg body weight, respectively. (A) mRNA level of IL-6, TNF-α and IL-10 in the lung tissue of rats. (B) ELISA analysis of protein levels of IL-6, TNF-α and IL-10 in the plasma of rats. Changes in (C) SOD activity and (D) MAD content in the plasma of rats. (E) mRNA and (F) protein expression of vWF, ICAM-1 and VCAM-1 in the lung tissue of rats. Data are shown as the mean ± SD. *P
    Figure Legend Snippet: Effect of Cy-3-g on inflammatory responses and oxidative stress in rats with pulmonary artery hypertension. Rats were randomly assigned into four groups (n=15): Control group, model group (MCT), Cy-3-g-L group and Cy-3-g-H group. On the first day of the experiments, the rats in the MCT, Cy-3-g-L and Cy-3-g-H groups were injected with MCT (60 mg/kg). On days 2–28, the rats in the Cy-3-g-L and Cy-3-g-H groups received a gavage of 200 and 400 mg/kg body weight, respectively. (A) mRNA level of IL-6, TNF-α and IL-10 in the lung tissue of rats. (B) ELISA analysis of protein levels of IL-6, TNF-α and IL-10 in the plasma of rats. Changes in (C) SOD activity and (D) MAD content in the plasma of rats. (E) mRNA and (F) protein expression of vWF, ICAM-1 and VCAM-1 in the lung tissue of rats. Data are shown as the mean ± SD. *P

    Techniques Used: Injection, Enzyme-linked Immunosorbent Assay, Activity Assay, Expressing

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Role of CCR7 on dendritic cell-mediated immune tolerance in the airways of allergy-induced asthmatic rats
    Article Snippet: ELISAAfter the animal model was successfully established, blood (4 ml) was extracted from the vena cava, anticoagulated with heparin and centrifuged (500 × g for 10 min at room temperature), and serum was collected. .. ELISA kits were used for measuring IFN-γ (cat. no. EK0374), IL-4 (cat. no. EK0406), IL-12 (cat. no. A01152-2), IL-10 (cat. no. EK0418), TGF-β (cat. no. EK0514; all Boster Biological Technology) and IgE (cat. no. E-EL-R0517c; Elabscience Biotechnology, Inc.) concentrations in serum and BALF supernatant, according to the manufacturer's protocol. ..

    Article Title: Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation
    Article Snippet: The stained sections were examined with a fluorescence microscope (Leica Biosystems), and images were captured with a charge coupled device (CCD) spot camera (Leica Biosystems). .. Enzyme linked immunosorbent assay (ELISA) The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology). .. Enzyme linked immunosorbent assay (ELISA) The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology).

    Article Title: Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
    Article Snippet: Cytokine assaysThe release of cytokines into the cell culture supernatants and BALF were detected by enzyme-linked immunosorbent assay (ELISA). .. The IL-1β, TNF-α, and IL-18 levels were detected using a mouse IL-1β ELISA Kit (R & D Systems, Inc., Minneapolis, MN, USA), rat IL-1β ELISA Kit (Boster, Wuhan, People’s Republic of China), rat IL-18 ELISA Kit (Boster), and mouse TNF-α ELISA Kit (BioLegend, San Diego, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantification Total RNA was extracted from the cultured cells and lung tissues after NiONP exposure using Trizol reagent (TaKaRa, Shiga, Japan), according to the manufacturer’s instructions. cDNA was then obtained using a Reverse Transcription Kit (TaKaRa).

    Article Title: Cyanidin-3-O-β-glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF-β1/p38 MAPK/CREB signaling pathway
    Article Snippet: .. ELISA determination of IL-6, TNF-α and IL-10According to the manufacturer's protocols, rat ELISA kits of IL-6 (cat. no. EK0412), TNF-α (cat. no. EK0526) and IL-10 (cat. no. EK0418; all purchased from Boster Biological Technology), standards and samples (tissue homogenate) were added to the plate (100 µl/well), and a blank was set as the control. .. The plate was covered with a membrane and incubated at 37°C for 1 h. After discarding the liquid, buffer A (100 µl/well) was added to the plate, and incubated at 37°C for 1 h. Next, the plate was washed 3 times with PBS with 1% Tween-20 and then incubated with buffer B (100 µl/well) at 37°C for 30 min. After rinsing 5 times, the plate was incubated with the chromogenic substrate 3,3′,5,5′-Tetramethylbenzidine for 10 min in the dark.

    Article Title: Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor 4/nuclear factor-κB signaling pathway
    Article Snippet: .. Serum was used to measure IL-10 (EK0418), IL-1β (EK0393), IL-6 (EK0412) and TNF-α (EK0526) levels using ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China) according to manufacturer's protocol. .. Following reperfusion and 2 weeks of Pter treatment, renal tissue samples were harvested and homogenized in RIPA buffer (Beyotime Institute of Biotechnology) containing 1% protease inhibitor cocktail.

    Article Title: Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg
    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) The amounts of cytokines TGF-β 1, IL-6, IL-10, and IL-17 in the collected sera were measured using sandwich ELISA. .. A rat TGF-β 1, IL-6, IL-10 ELISA kit (BOSTER) and rat IL-17A ELISA kit (DAKEWE) were used according to the manufacturer's instructions to determine the TGF-β1, IL-6, IL-10, and IL-17 content in the serum. .. The absorbance at 450 nm was measured by using an ELISA microplate reader.

    Centrifugation:

    Article Title: Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation
    Article Snippet: The stained sections were examined with a fluorescence microscope (Leica Biosystems), and images were captured with a charge coupled device (CCD) spot camera (Leica Biosystems). .. Enzyme linked immunosorbent assay (ELISA) The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology). .. Enzyme linked immunosorbent assay (ELISA) The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology).

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  • 96
    Boster Bio il 10
    Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase <t>IL-10</t> expression of rats with nucleus pulposus (NP) (** P
    Il 10, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 10/product/Boster Bio
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 10 - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase IL-10 expression of rats with nucleus pulposus (NP) (** P

    Journal: The Korean Journal of Pain

    Article Title: Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation

    doi: 10.3344/kjp.2021.34.1.47

    Figure Lengend Snippet: Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase IL-10 expression of rats with nucleus pulposus (NP) (** P

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology).

    Techniques: Expressing

    The concentrations of cytokines in rats. (a) The concentration of IL-6, n = 8. (b) The concentration of IL-17, n = 8. (c) The concentration of TGF- β 1, n = 8. (d) The concentration of IL-10, n = 8. Data are expressed as the means ± SD values. ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg

    doi: 10.1155/2020/5375981

    Figure Lengend Snippet: The concentrations of cytokines in rats. (a) The concentration of IL-6, n = 8. (b) The concentration of IL-17, n = 8. (c) The concentration of TGF- β 1, n = 8. (d) The concentration of IL-10, n = 8. Data are expressed as the means ± SD values. ∗ P

    Article Snippet: A rat TGF-β 1, IL-6, IL-10 ELISA kit (BOSTER) and rat IL-17A ELISA kit (DAKEWE) were used according to the manufacturer's instructions to determine the TGF-β1, IL-6, IL-10, and IL-17 content in the serum.

    Techniques: Concentration Assay

    NiONPs induce caspase-1-dependent cytokine secretion in RAW264.7 cells. Notes: ( A – C ) The pro-caspase-1 and pro-IL-1β levels in cell lysates (input) and processed caspase-1 and IL-1β levels in culture SNs after treatment with different doses (0 µg/cm 2 , 1 µg/cm 2 , 2 µg/cm 2 , and 4 µg/cm 2 ) of NiONPs were analyzed by Western blotting. ( D ) Caspase-1 activity in RAW264.7 cells. ( E ) Detection of IL-1β expression in the SN by ELISA. * P

    Journal: International Journal of Nanomedicine

    Article Title: Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

    doi: 10.2147/IJN.S106912

    Figure Lengend Snippet: NiONPs induce caspase-1-dependent cytokine secretion in RAW264.7 cells. Notes: ( A – C ) The pro-caspase-1 and pro-IL-1β levels in cell lysates (input) and processed caspase-1 and IL-1β levels in culture SNs after treatment with different doses (0 µg/cm 2 , 1 µg/cm 2 , 2 µg/cm 2 , and 4 µg/cm 2 ) of NiONPs were analyzed by Western blotting. ( D ) Caspase-1 activity in RAW264.7 cells. ( E ) Detection of IL-1β expression in the SN by ELISA. * P

    Article Snippet: The IL-1β, TNF-α, and IL-18 levels were detected using a mouse IL-1β ELISA Kit (R & D Systems, Inc., Minneapolis, MN, USA), rat IL-1β ELISA Kit (Boster, Wuhan, People’s Republic of China), rat IL-18 ELISA Kit (Boster), and mouse TNF-α ELISA Kit (BioLegend, San Diego, CA, USA), following the manufacturer’s instructions.

    Techniques: Western Blot, Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay

    Effect of DM and MLT on the serum levels of pro-inflammatory mediators. Tumor necrosis factor alpha (TNF-α) (a), interleukin 6 (IL-6) (b), interleukin 1 beta (IL-1β) (c) and anti-inflammatory cytokine interleukin 10 (IL-10) (d) of rats in the different groups. Values are expressed as means ± SEM; (n = 5). (∗, ∗∗ and ∗∗∗ indicate statistical significance at P

    Journal: Heliyon

    Article Title: Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

    doi: 10.1016/j.heliyon.2021.e06474

    Figure Lengend Snippet: Effect of DM and MLT on the serum levels of pro-inflammatory mediators. Tumor necrosis factor alpha (TNF-α) (a), interleukin 6 (IL-6) (b), interleukin 1 beta (IL-1β) (c) and anti-inflammatory cytokine interleukin 10 (IL-10) (d) of rats in the different groups. Values are expressed as means ± SEM; (n = 5). (∗, ∗∗ and ∗∗∗ indicate statistical significance at P

    Article Snippet: Low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C) levels were determined using Friedewald's & 3) [ ]. (2) LDL-C = TC − (HDL-C + VLDL-C) (3) VLDL-C = TG/5Interleukin 6 (IL-6; Catalog #: EK0412) with a detection range (62.5pg/ml-4000 pg/ml), interleukin 1 beta (IL-1b; Catalog #: EK0393) with a detection range (31.2pg/ml-2000 pg/ml), interleukin 10 (IL-10; Catalog #: EK0418) with a detection range (31.2pg/ml-2000 pg/ml), tumor necrosis factor alpha (TNF-α; Catalog #: RTFI01177) with a detection range (15.625–1000 pg/ml) and troponin T (Catalog #: KT-30669) with a detection range (0.078–5 ng/ml) in serum were assessed using kits provided by BosterBio (California, USA), ElisaGenie (London, UK) and Kamiya Biomedical (Seattle, USA), respectively.

    Techniques: