mouse igf 1 elisa kit picokine  (Boster Bio)


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    Name:
    Mouse IGF 1 ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK0378
    Price:
    369.0
    Category:
    ELISA Kits
    Reactivity:
    Mouse
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: E.coli; Immunogen sequence: G49-A118
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    Structured Review

    Boster Bio mouse igf 1 elisa kit picokine
    Mouse IGF 1 ELISA Kit PicoKine

    https://www.bioz.com/result/mouse igf 1 elisa kit picokine/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igf 1 elisa kit picokine - by Bioz Stars, 2021-06
    94/100 stars

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    1) Product Images from "Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality"

    Article Title: Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22010451

    Western blot analysis of IGF-1R, Akt and Nrf2 activation in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A , B ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt, phosphorylated Nrf2 (p.Nrf2, Ser40 residue), β–Tubulin and β–Actin in heart protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. ( C , D ) Quantification of the IGF-1R and Nrf2 activation. The Y axis in graphs represents the ratios of the respective measured pixel intensities of Western blot bands. Total number of analyzed samples from both CD2F1 and C3H strains: sham— n = 6; decedent— n = 6; survivor n = 3. Data analyzed by Student’s t -test and are presented as mean ± SEM, * p
    Figure Legend Snippet: Western blot analysis of IGF-1R, Akt and Nrf2 activation in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A , B ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt, phosphorylated Nrf2 (p.Nrf2, Ser40 residue), β–Tubulin and β–Actin in heart protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. ( C , D ) Quantification of the IGF-1R and Nrf2 activation. The Y axis in graphs represents the ratios of the respective measured pixel intensities of Western blot bands. Total number of analyzed samples from both CD2F1 and C3H strains: sham— n = 6; decedent— n = 6; survivor n = 3. Data analyzed by Student’s t -test and are presented as mean ± SEM, * p

    Techniques Used: Western Blot, Activation Assay, Irradiation, Mouse Assay

    Serum IGF-1 and Nitric Oxide (NO) levels in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) ELISA analysis of serum IGF-1 levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 7 for sham animals of both strains, n = 8 for decedent animals of both strains, n = 3 for survivors of both strains. ( B ) Serum nitric oxide levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 8 for sham animals of both strains, n = 8 for CD2F1 decedents, n = 10 for C3H/HeN decedents, n = 3 for survivors of both strains. Data analyzed by student’s t -test. Results presented as mean ± SEM, *** p
    Figure Legend Snippet: Serum IGF-1 and Nitric Oxide (NO) levels in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) ELISA analysis of serum IGF-1 levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 7 for sham animals of both strains, n = 8 for decedent animals of both strains, n = 3 for survivors of both strains. ( B ) Serum nitric oxide levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 8 for sham animals of both strains, n = 8 for CD2F1 decedents, n = 10 for C3H/HeN decedents, n = 3 for survivors of both strains. Data analyzed by student’s t -test. Results presented as mean ± SEM, *** p

    Techniques Used: Irradiation, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Western blot analysis of IGF-1R, Akt and Nrf2 in lung and kidney samples from sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt and ( C ) phosphorylated Nrf2 (p.Nrf2, Ser40) in lung protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. β–Actin used as loading control. ( B , D ) Quantification of IGF-1R and Nrf2 activation in the lung samples. IGF-1R activation is determined by calculating the ratio of the pixel intensity of p.IGF-1R to total IGF-1R levels. Nrf2 activation is determined by calculating the ratio of pixel intensity of the p.Nrf2 to respective β–Actin band. ( E ) Western blot analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R and total Akt in kidney protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. GAPDH is used as loading control. Total number of analyzed samples for both strains: n = 6 for sham, n = 6 for decedents, n = 3 for survivors. Results analyzed by Student’s t -test; presented as mean ± SEM, * p
    Figure Legend Snippet: Western blot analysis of IGF-1R, Akt and Nrf2 in lung and kidney samples from sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt and ( C ) phosphorylated Nrf2 (p.Nrf2, Ser40) in lung protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. β–Actin used as loading control. ( B , D ) Quantification of IGF-1R and Nrf2 activation in the lung samples. IGF-1R activation is determined by calculating the ratio of the pixel intensity of p.IGF-1R to total IGF-1R levels. Nrf2 activation is determined by calculating the ratio of pixel intensity of the p.Nrf2 to respective β–Actin band. ( E ) Western blot analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R and total Akt in kidney protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. GAPDH is used as loading control. Total number of analyzed samples for both strains: n = 6 for sham, n = 6 for decedents, n = 3 for survivors. Results analyzed by Student’s t -test; presented as mean ± SEM, * p

    Techniques Used: Western Blot, Irradiation, Mouse Assay, Activation Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality
    Article Snippet: For each group of animals the protein lysates from the tissues of at least 6 random animals (for sham and irradiated decedents) and 3 individual irradiated survivor animals were analyzed three times or more by Western blot technique. .. ELISA, Peroxide, ATP and Nitric Oxide MeasurementsFrozen serum samples were thawed on ice and subsequently diluted 1:100 times to measure the concentration of IGF-1 using the mouse IGF-1 ELISA Kit PicoKine™ (Boster Bio, Pleasanton, CA, USA) by following the vendor instructions. .. For tissue peroxide measurements, 25 mg of frozen heart powder was homogenized in 300 µL of lysis buffer containing 0.1 M KCl and 0.1 M Na2 HPO4 ·7H2 O.

    Concentration Assay:

    Article Title: Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality
    Article Snippet: For each group of animals the protein lysates from the tissues of at least 6 random animals (for sham and irradiated decedents) and 3 individual irradiated survivor animals were analyzed three times or more by Western blot technique. .. ELISA, Peroxide, ATP and Nitric Oxide MeasurementsFrozen serum samples were thawed on ice and subsequently diluted 1:100 times to measure the concentration of IGF-1 using the mouse IGF-1 ELISA Kit PicoKine™ (Boster Bio, Pleasanton, CA, USA) by following the vendor instructions. .. For tissue peroxide measurements, 25 mg of frozen heart powder was homogenized in 300 µL of lysis buffer containing 0.1 M KCl and 0.1 M Na2 HPO4 ·7H2 O.

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    Boster Bio mouse igf 1 elisa kit picokine
    Western blot analysis of IGF-1R, Akt and Nrf2 activation in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A , B ) Analysis of phosphorylated <t>IGF-1</t> receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt, phosphorylated Nrf2 (p.Nrf2, Ser40 residue), β–Tubulin and β–Actin in heart protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. ( C , D ) Quantification of the IGF-1R and Nrf2 activation. The Y axis in graphs represents the ratios of the respective measured pixel intensities of Western blot bands. Total number of analyzed samples from both CD2F1 and C3H strains: sham— n = 6; decedent— n = 6; survivor n = 3. Data analyzed by Student’s t -test and are presented as mean ± SEM, * p
    Mouse Igf 1 Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igf 1 elisa kit picokine/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igf 1 elisa kit picokine - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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    Western blot analysis of IGF-1R, Akt and Nrf2 activation in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A , B ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt, phosphorylated Nrf2 (p.Nrf2, Ser40 residue), β–Tubulin and β–Actin in heart protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. ( C , D ) Quantification of the IGF-1R and Nrf2 activation. The Y axis in graphs represents the ratios of the respective measured pixel intensities of Western blot bands. Total number of analyzed samples from both CD2F1 and C3H strains: sham— n = 6; decedent— n = 6; survivor n = 3. Data analyzed by Student’s t -test and are presented as mean ± SEM, * p

    Journal: International Journal of Molecular Sciences

    Article Title: Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality

    doi: 10.3390/ijms22010451

    Figure Lengend Snippet: Western blot analysis of IGF-1R, Akt and Nrf2 activation in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A , B ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt, phosphorylated Nrf2 (p.Nrf2, Ser40 residue), β–Tubulin and β–Actin in heart protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. ( C , D ) Quantification of the IGF-1R and Nrf2 activation. The Y axis in graphs represents the ratios of the respective measured pixel intensities of Western blot bands. Total number of analyzed samples from both CD2F1 and C3H strains: sham— n = 6; decedent— n = 6; survivor n = 3. Data analyzed by Student’s t -test and are presented as mean ± SEM, * p

    Article Snippet: ELISA, Peroxide, ATP and Nitric Oxide MeasurementsFrozen serum samples were thawed on ice and subsequently diluted 1:100 times to measure the concentration of IGF-1 using the mouse IGF-1 ELISA Kit PicoKine™ (Boster Bio, Pleasanton, CA, USA) by following the vendor instructions.

    Techniques: Western Blot, Activation Assay, Irradiation, Mouse Assay

    Serum IGF-1 and Nitric Oxide (NO) levels in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) ELISA analysis of serum IGF-1 levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 7 for sham animals of both strains, n = 8 for decedent animals of both strains, n = 3 for survivors of both strains. ( B ) Serum nitric oxide levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 8 for sham animals of both strains, n = 8 for CD2F1 decedents, n = 10 for C3H/HeN decedents, n = 3 for survivors of both strains. Data analyzed by student’s t -test. Results presented as mean ± SEM, *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality

    doi: 10.3390/ijms22010451

    Figure Lengend Snippet: Serum IGF-1 and Nitric Oxide (NO) levels in sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) ELISA analysis of serum IGF-1 levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 7 for sham animals of both strains, n = 8 for decedent animals of both strains, n = 3 for survivors of both strains. ( B ) Serum nitric oxide levels in sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. n = 8 for sham animals of both strains, n = 8 for CD2F1 decedents, n = 10 for C3H/HeN decedents, n = 3 for survivors of both strains. Data analyzed by student’s t -test. Results presented as mean ± SEM, *** p

    Article Snippet: ELISA, Peroxide, ATP and Nitric Oxide MeasurementsFrozen serum samples were thawed on ice and subsequently diluted 1:100 times to measure the concentration of IGF-1 using the mouse IGF-1 ELISA Kit PicoKine™ (Boster Bio, Pleasanton, CA, USA) by following the vendor instructions.

    Techniques: Irradiation, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Western blot analysis of IGF-1R, Akt and Nrf2 in lung and kidney samples from sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt and ( C ) phosphorylated Nrf2 (p.Nrf2, Ser40) in lung protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. β–Actin used as loading control. ( B , D ) Quantification of IGF-1R and Nrf2 activation in the lung samples. IGF-1R activation is determined by calculating the ratio of the pixel intensity of p.IGF-1R to total IGF-1R levels. Nrf2 activation is determined by calculating the ratio of pixel intensity of the p.Nrf2 to respective β–Actin band. ( E ) Western blot analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R and total Akt in kidney protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. GAPDH is used as loading control. Total number of analyzed samples for both strains: n = 6 for sham, n = 6 for decedents, n = 3 for survivors. Results analyzed by Student’s t -test; presented as mean ± SEM, * p

    Journal: International Journal of Molecular Sciences

    Article Title: Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality

    doi: 10.3390/ijms22010451

    Figure Lengend Snippet: Western blot analysis of IGF-1R, Akt and Nrf2 in lung and kidney samples from sham and LD 70/30 irradiated CD2F1 and C3H/HeN adult male mice. ( A ) Analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R, phosphorylated Akt (p.Akt, Ser473), total Akt and ( C ) phosphorylated Nrf2 (p.Nrf2, Ser40) in lung protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. β–Actin used as loading control. ( B , D ) Quantification of IGF-1R and Nrf2 activation in the lung samples. IGF-1R activation is determined by calculating the ratio of the pixel intensity of p.IGF-1R to total IGF-1R levels. Nrf2 activation is determined by calculating the ratio of pixel intensity of the p.Nrf2 to respective β–Actin band. ( E ) Western blot analysis of phosphorylated IGF-1 receptor (p.IGF1R, Tyr1135/1136), total IGF-1R and total Akt in kidney protein extracts from sham, irradiated decedent (Decd.) and irradiated survivor (Surv.) mice. GAPDH is used as loading control. Total number of analyzed samples for both strains: n = 6 for sham, n = 6 for decedents, n = 3 for survivors. Results analyzed by Student’s t -test; presented as mean ± SEM, * p

    Article Snippet: ELISA, Peroxide, ATP and Nitric Oxide MeasurementsFrozen serum samples were thawed on ice and subsequently diluted 1:100 times to measure the concentration of IGF-1 using the mouse IGF-1 ELISA Kit PicoKine™ (Boster Bio, Pleasanton, CA, USA) by following the vendor instructions.

    Techniques: Western Blot, Irradiation, Mouse Assay, Activation Assay